• 동아시아식생활학회지
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• 제20권5호
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• pp.751-758
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• 2010

Prunus mume is well known contain many functional materials that play beneficial roles in the human body. Studies have found that many organic acids and polyphenol compounds exist in Prunus mume. In this study, content of total polyphenols and flavonoids, antioxidative activity and cytotoxicity of ethanol extract from Prunus mume were measured Contents of total polyphenolic and total flavonoid compounds in ethanol extract from Prunus mume were 16.92 mg/g and 59.95 mg/g, respectively. The $IC_{50}$ of ethanol extract from Prunus mume were $237.72 {\mu}g$/assay and $239.58 {\mu}g$/assay by DPPH and ABTS radical cation scavenging test, respectively. Additionally, ferric ion reducing antioxidant power (FRAP) value of ethanol extract from Prunus mume was 0.94 mM ($FeSO_4$ eq.) by $800 {\mu}g$/assay. Cytotoxicity of Prunus mume against five kinds of cancer cell lines increased as the extract concentration increased Especially, cytotoxicity of the ethanol extract against A-549 (lung cancer line) was higher than that against any other cancer cell line by both MTT and SRB assay. These results show that ethanol extract of Prunus mume has considerably high antioxidative and cytotoxic activities.

• Journal of Nutrition and Health
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• 제35권9호
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• pp.926-931
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• 2002

The antimicrobial properties of extracts of Prunus mume were tested on Salmonella paratyphimurium and S. typhimurium. First, the Prumus mume was extracted with methanol at several temperatures, and then fractionation of the methanol extracts from Prunus mume was carried out by using petroleum ether, chloroform, ethyl acetate or butanol. Secondly, absorption column chromatoraphy (using a Diaion HP 20) was conducted to eliminate some water soluble materials that might inhibit the antimicrobial activity of some extracts. The antimicrobial activitry of each of the Prunus mume extracts was determined using a paper disc method against several food-borne pathogens, The growth inhibition curve was determine using butanol extracts of Prunus mume against Salmonella typhimurium. The extraction temperature did not have any significant effect on the yield of the extract or on the level of antimicrobial activity. The butanol extract of Prunus mume showed strong antimicrobial activity against Salmonella paratyphimurium and S. typhimurium; a 1,000 ppm of butanol extract of Prunus mume retared the growth of S. typhimurium up to 36 hours. (Korean J Nutrition 35(9) : 926~931, 2002)

• 한국치위생학회지
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• 제14권1호
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• pp.109-115
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• 2014

Objectives : Prunus mume has been used for the folk medicine from old times. The purpose of the study was to investigate the antimicrobial activity of prunus mume extract to various oral microbes. Methods : This study was carried out to examine the antimicrobial effect of Prunus mume extract against oral microbes. Data were collected using a Dentocult SM Strip mutans and Dentocult LB Strip mutans from April 5 to May 4, 2013. A total of 36 experimental and 32 control group were selected for this study. Results : The MIC of Prunus mume extract was tested for 0.39% in S. mutans, S. salivarius and S. auerus, 0.78% in S. mitis, S. equi and E. coli. In vivo, experimental group showed significantly the lower Streptococcus mutans levels by the use of the gum contained Prunus mume extract from 15 days compared with control group(p=0.012). The reduction was more significant in Lactobacilli level of the experimental group than the control group(p=0.022). Conclusions : These findings suggest that the oral products containing Prunus mume extracts is effective in preventing oral diseases.

• 동아시아식생활학회지
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• 제12권3호
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• pp.241-248
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• 2002

The dried Prunus mume, an alkaline food abundant in organic acids (citric acid, malic acid and tartaric acid), has been largely used in both folklore remedies and Chinese herbal medicine for a long time. This study was performed to investigate the antimicrobial activity of the dried Prunus mume. The fractionation of the methanol extracts from Prunus mume was conducted using petroleum ether, chloroform, ethyl acetate and butanol, respectively. The antimicrobial activity of the Prunus mume extracts was then determined against food-borne pathogens using a paper disc method. The ethyl acetate extract showed the strongest antimicrobial activity against the eigth food-born pathogens used in this present study. Diaion HP 20 column chromatography was performed to remove some sugars that might inhibit the antimicrobial activity of Prunus mume. The strongest antimicrobial activity of ethyl acetate fraction of Prunus mume was shown against Staphylococus aureus. The growth inhibition curve was determined using ethyl acetate extracts of Prunus mume against Staphylococus aureus, which showed the growth inhibition up to 72 hours at 1,000 ppm concentration.

• 한국식품영양학회지
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• 제17권4호
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• pp.382-387
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• 2004

영양적, 기능적 면에서 우수성을 지닌 클로렐라를 소재로 한 음료와 여기에 매실을 첨가한 혼합음료 즉, 클로렐라 추출물 5%액과 10%액의 음료와 클로렐라 추출물 5%액에 매실액 0.2%와 0.4%액을 혼합한 음료 등 4종류를 제조하고, 그 특성과 안전성, 그리고 선호도 등을 조사하였다. 그 결과를 요약하면 다음과 같다. 영양적으로는 단백질, 당질, 무기질(Zn, Fe, Mn, Ca, Na, Mg, K, Cu) 등이 상당량 들어 있으며, 지질은 검출되지 않았는데, 이는 클로렐라와 매실에 지질함량이 적기 때문인 것으로 생각되며, 당질이 많이 검출된 것은 첨가물로 설탕을 넣었기 때문으로 생각된다. 색도 검사에서는 클로렐라 추출물 10%액이 5%액보다 명도, 적색도, 황색도 등이 모두 감소했으며, 클로렐라 추출물 5%액에 매실액 0.2%와 0.4%액을 혼합한 경우에는 0.4%액이 0.2%액에서 보다 명도, 적색도는 감소한 반면 황색도에서는 차이가 없었다. 안전성 확인을 위해 각 제품에 대하여 중금속 검사, 세균 및 대장균의 검사 등을 실시한 결과 모두 안전한 것으로 나타났다. 관능검사 결과에서는 색깔, 맛, 향, 선호도에서 클로렐라 추출물 5%액에 매실액 0.4%을 첨가한 음료가 가장 좋은 것으로 나타났으며, 다음으로는 클로렐라 추출물 5%액에 매실액 0.2%를 첨가한 것으로 나타났고, 가장 좋지 않은 것으로는 클로렐라 추출물 10%액인 것으로 나타났다.

• Plant Resources
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• 제8권3호
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• pp.188-193
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• 2005

In order to discern the possibility of functional food product or ingredient of a new medicine, the leaf parts and fruit parts of Prunus mume was partitioned with various solvents and their antioxidative activity was measured. When the antioxidative activity of MeOH extracts of leaf parts and fruit parts of Korea and China was compared, all of them showed the highest antioxidative activity in EtOAc fraction. In case of Korean Prunus mume leaf parts showed that quantity required for $RC_{50}$ to be $27.04{\mu}g$ in EtOAc fraction and in case of China Prunus mume leaf parts, it was $23.31\;{\mu}g$ which is similar to that of ${\alpha}$-tocopherol ($22.14\;{\mu}g$) and showed the highest activation. In case of Prunus mume fruit parts MeOH extract, Korean fruit showed $29.16\;{\mu}g$, and Chinese fruit showed $31.21\;{\mu}g$ in EtOAc fraction and thus Korean fruit extract showed a higher activity of antioxidant than the Chines fruit extract. When the antioxidative activity between the fruit parts and leaf parts of Prunus mume was compared, the leaf parts showed a higher antioxidative activity.

• 한국식품영양과학회지
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• 제19권1호
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• pp.21-26
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• 1990

The aim of this study is to investigate effects of Prunus mume extract on gastric secretion in rats and experimentally carbon tetrachloride-induced liver damage of rabbits. The groups of Prunus mume extract showed excellent increasing effect in total acidity and peptic activity and decreasing effect on gastric volume than the control group whereas clear differences were not found between sample adminstered group and control group in gastric pH. In liver function test Prunus mume extract exhibited rapid recuperation of liver function that is sGPT activity showed an apparant decreasing effect from the 6th day total cholesterol and alkaline phosphatase level from the 10th day respectively compared to the control group in carbon tetrachloride-intoxicated rabbits. It is, is suggested that Pruns mume extract can be used as the stomachics to promote the gastric digestion by increasing the peptic activity and health food to shorten the recovery time from liver diseases.

• 한국지역사회생활과학회지
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• 제15권4호
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• pp.3-10
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• 2004

본 연구에서는 매실농축액을 동치미에 첨가함으로써 동치미의 발효숙성에 미치는 영향을 조사하였다. 매실농축액을 0, 0.05, 0.1, 0.2 및 0.3% 첨가하여 제조한 동치미를 $10^{\circ}C$에서 30일간 숙성시키면서 이화학적 특성 및 미생물학적 특성을 조사하였다. pH는 발효가 진행되면서 감소하고, 발효 6~8일에 pH 3.8 - 4.2를 나타내었다. 산도의 변화는 매실농축액 첨가에 의해 감소하였다 산도의 증가 및 pH의 감소는 매실농축액 첨가에 비해 무첨가구에서 보다 빨리 나타났다. 총 비타민 C의 함량은 발효 10일까지 지속적 증가를 보이다가 점차 감소하였다. 환원당 함량은 발효 10일까지 증가를 보였으며 특히, 매실농축액 0.3% 첨가구가 가장 높았다. 동치미 발효 시 총균수와 젖산균의 경우는 매실농축액 첨가에 따라 생육이 억제되었다. 관능검사는 매실농축액 첨가구가 발효 10일에 좋은 향기와 전체적 기호를 보였다. 이상의 결과로 매실농축액이 동치미 내에 미생물들을 억제하여 동치미 발효숙성을 지연시키는 효과를 나타내고 있다.

• 한국식품저장유통학회지
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• 제11권1호
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• pp.71-78
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• 2004

매실 에탄올추출물이 알코올투여로 유발된 횐쥐의 간손상에 미치는 영향을 알아보기 위하여 실험동물을 정상군, 매실 에탄올추출물 200 mg/kg 투여군, 알코올투여군(35% ethanol 10 mL/kg, b.w./day), 알코올 및 매실 에탄올추출물 200 mg/kg 병합투여군과 알코올 및 매실 에탄올추출물 400 mg/kg 병합투여군의 5군으로 나누어 6주간 사육하였고, 체중증가율 및 식이효율, 혈청중 ALT와 AST활성, 간손상 억제 효과를 확인하기 위해 SOD, catalase, xanthi ne oxidase 및 GSH-Px활성을 측정하고, 지질과산화물인 thiobarbituric acid reactant(TBARS) 및 GSM함량에 미치는 영향을 측정하였다. 그 결과, 매실 에탄올추출물이 알코올투여로 증가된 유리기해독계 효소인 GSH-Px활성을 유의성 있게 감소시키고 비효소적 항산화작용을 나타내는 GSH함량을 증가시킴으로서 지질과산화물에 대한 방어력이 증강됨을 보였고, 알코올 투여로 상승된 ALT 및 AST활성을 유의성있게 감소시켜 매실 에탄올추출물이 에탄올에 의한 지방간 또는 손상된 간세포를 회복시키는 작용이 있는 것으로 추정되었다.

• 동아시아식생활학회지
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• 제9권2호
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• pp.214-222
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• 1999

Prunus mume has been used for the folk medicine by many old civilizations to treat food-borne diseases or enteric disorders. The purpose of this study was to investigate the antimicrobial activity of beverages containing Prunus mume extracts against Staphylococcus aureus, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Escherichia coli and Pseudomonas aeruginosa. Seven different types of Prunus mume extracts containing beverages have been prepared for the test in which minimum inhibitory concentration for each microorganism has been determined by serial dilution method using either TSA or TSB medium. Pseudomonas aeruginosa showed least resistance and 0.3g/$m\ell$ concentrations of 5% Prunus mume extracts containing beverage had antimicrobial property against the organism. Beverages containing more than 15% of Prunus mume extracts showed antimicrobial activity against all tested microorganisms at the MIC value of less than 0.25g/$m\ell$. The effect of Prunus mume on the growth of food-borne pathogens has been also studied using a spectrophotometer. In the growth assay, each of the Prunus mume extracts containing beverage was added to the medium at the concentration of 25% (v/v). Beverage containing 20% Prunus mume extracts showed inhibitory effect on the growth of all tested microorganisms.