• 약학회지
• /
• 제36권2호
• /
• pp.115-119
• /
• 1992

The possibility as an anti-inflammatory drug of Prunus davidiana which have been used in Korean folk medicine for treatment of neuritis and rheumatism was investigated on carrageenin-induced paw edema in rats. The MeOH extract exhibited potent inhibitory effect on carrageenin-edema rats when topically applicated. On the other hand, it did not show any effect when orally tested. It also had no influences on isolated ileum of guinea pig and anti-platelet aggregating activities. Thus, it appears from the present findings that the MeOH extract of P. davidiana may be utilizable only for the external treatment of inflammed sites. This study was also conducted to further isolation of flavonoids from the EtOAc soluble fraction and characterized as persiconin and isosakuranin by spectrometric analysis.

• 생약학회지
• /
• 제24권4호
• /
• pp.299-303
• /
• 1993

The antioxidant activity of methanol extracts of plants and marine algae was tested by using 1,1-diphenyl-2-picrylhydrazyl(DPPH). Five plant extracts(Prunus davidiana, Eriobotrya japonica, Artemisia iwayomogi, Stirodella tolyrrhiza and Ulmus davidiana) and two algae (Ecklonia stolonifera and Symphycoladia latiuscula) were found to be the most effective in DPPH radical scavenging activity. The methanol extract obtained from the stems of Prunus davidiana was fractionated with several solvents. The ethylacetate soluble fraction exhibiting the strongest antioxidant activity was further purified by repeated silica gel and Sephadex LH-20 column chromatography. Antioxidant flavonoids and flavonoid glycosides were isolated and the most active ones was identified as (+)-catechin by MS, $^1H-NMR$ and $^{13}C-NMR$. Its antioxidant activity was higher than that oil vitamin C.

• Archives of Pharmacal Research
• /
• 제26권10호
• /
• pp.809-815
• /
• 2003

Since reactive oxygen species (ROS) and hydroxyl radicals ($^-OH$) play an important role in the pathogenesis of many human degenerative diseases, much attention has focused on the development of safe and effective antioxidants. Preliminary experiments have revealed that the methanol (MeOH) extract of the stem of Prunus davidiana exerts inhibitory/scavenging activities on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals, total ROS and peroxynitrites ($ONOO^-$). In the present study, the antioxidant activities of this MeOH extract and the organic solvent-soluble fractions, dichloromethane (CH$_2$Cl$_2$), ethyl acetate (EtOAc), and n-butanol (n-BuOH), and the water layer of P. davidiana stem were evaluated for the potential to inhibit $^-OH$ and total ROS generation in kidney homogenates using 2',7'-dichlorodihydrofluorescein diacetate (DCHF-DA), and for the potential to scavenge authentic $ONOO^-$. We also evaluated the inhibitory activity of seven flavonoids isolated from P. davidiana stem, kaempferol, kaempferol 7-Ο-$\beta$-D-glucoside, (+)-catechin, dihydrokaempferol, hesperetin 5-Ο-$\beta$-D-glucoside, naringenin and its 7-Ο-$\beta$-D-glucoside, on the total ROS, $^-OH$ and $ONOO^-$ systems. For the further elucidation of the structure-inhibitory activity relationship of flavonoids on total ROS and 'OH generation, we measured the antioxidant activity of sixteen flavonoids available, including three active flavonoids isolated from P. davidiana, on the total ROS and 'OH systems. We found that the inhibitory activity on total ROS generation increases in strength with more numerous hydroxyl groups on their structures. Also, the presence of an ortho-hydroxyl group, whether on the Aring or S-ring, and a 3-hydroxyl group on the C-ring increased the inhibitory activity on both total ROS and $^-OH$ generation.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
• /
• pp.200.3-200.3
• /
• 2003

Reactive oxygen species(ROS) are produced at a high rate continuously as a by-product of aerobic metabolism. Several lines of evidence provided that ROS appears to cause to develop aging and various diseases. High level of hyaluronic acid with decreased molecular weight has been detected in patients with inflammatory diseases including rheumatoid arthritis. Hyaluronidase is an endohexosaminidase that initiates the degradation of hyaluronic acid with high molecular weight. Prunus persica Batsch var. davidiana Maximowicz has been known as a korean folk medicine for treatment of neuritis and rheumatism. (omitted)

• Journal of Applied Biological Chemistry
• /
• 제64권2호
• /
• pp.133-140
• /
• 2021

본 연구에서는 개복숭아꽃과 흰색, 분홍색 매화 꽃의 항균 활성과 항산화, 미백 효과에 대해 알아보고자 하였다. 먼저, 3종의 꽃추출물들은 4종의 균주 중에 S. aureus에 대해 우수한 항균 활성을 나타냈다. 흰매화 클로로포름층은 0.5 mg/mL 농도에서 S. aureus에 대해 84%의 항균 활성을 보였고 개복숭아꽃과 분홍매화 클로로포름층도 0.5 mg/mL 농도에서 각각 49, 30%의 항균 효과를 나타냈다. 3종의 꽃추출물들은 P. aeruginosa에 대해서도 항균 효과를 보였다. 개복숭아꽃 클로로포름층과 분홍매화 클로로포름층은 0.5 mg/mL 농도에서 각각 36, 30%의 항균 활성을 보였다. 이 추출물들은 S. epidermidis와 E. coli에 대해서는 항균 활성을 보이지 않았다. 3종의 꽃추출물들은 HaCaT cell의 생존에 크게 영향을 미치지 않았다. 분홍매화 증류수층은 20, 40 ㎍/mL 농도에서 항산화 효과를 보였다. 분홍매화 에틸아세테이트층도 40 ㎍/mL에서 항산화 효과를 보였는데 이는 글루타치온 보다 우수하였다. 꽃추출물들은 B16F10 cell의 생존율에는 영향을 미치지 않았다. 꽃추출물들의 미백 효과는 개복숭아꽃 클로로포름층 40 ㎍/mL에서 18%가 나타났다. 이러한 결과들을 바탕으로 3종의 꽃추출물들은 항균 활성과 항산화, 미백 효과가 있는 원료로 판단할 수 있었다.

• 한국약용작물학회지
• /
• 제12권4호
• /
• pp.289-294
• /
• 2004

인류의 수명이 연장되고 노령화 사회로 발전됨에 따라 다양한 성인병이 야기되고 있고, 이들 성인병의 원인이 활성산소에 의한 것으로 밝혀짐에 따라 다양한 성인병을 예방 치료할 수 있는 안전하고 우수한 항산화 및 항염증 물질을 천연물로부터 개발하기 위한 연구의 일환으로 자생식물의 하나인 야생복숭아 가지에 대하여 그들의 MeOH 엑스를 필두로 한 분획물 엑스를 대상으로 항산화 및 항염증 효과를 조사하였다. DPPH 법을 사용하여 야생복숭아 가지의 분획물 엑스에 대한 1차 라디칼 소거작용 검색 시험을 한 결과 야생복숭아 가지 의 n-BuOH 엑스와 EtOAc 엑스가 가장 강한 항산화 활성을 나타내었다. 1차 실험에서 얻어진 결과를 토대로 야생복숭아에 대하여 보다 상세한 항산화 효과를 검토하기 위하여 Ferric-Thiocyanate 법을 이용한 지질 과산화 억제작용 시험한 결과 야생복숭아 가지 의 EtOAc 엑스가 천연 항산화제인 tocopherol 및 합성 항산화제인 BHA 보다 우수한 탁월한 항산화 효과를 나타내었고, Rancimat법에 의한 지질 산패 억제 실험을 실시한 결과 또한 야생복숭아 가지의 EtOAc 엑스가 tocopherol보다 우수하고 BHA보다는 약간 우수한 강력한 지질 산패 억제 효과를 보였다. Hyaluronidase 저해에 의한 항염증 효과 시험에서는 항산화 실험에서 전반적으로 우수한 항산화 효과를 보인 야생복숭아 가지의 EtOAc 엑스가 ketoprofen보다는 약하나 ibuprofen과 indomethacin보다는 우수한 항염증 효과를 나타내었다. 이상의 실험 결과는 야생복숭아의 항산화 및 항염증 효과의 활성 주성분 분획은 EtOAc 엑스임을 시사하였다.

• 한국조리학회지
• /
• 제23권8호
• /
• pp.163-172
• /
• 2017

In order to investigate the quality characteristics of Prunus persica Batsch var. davidiana Max. preserved in sugar, the quality characteristics were examined by dividing them into four groups: PS-A (P. persica preserved in sugar mixed with sucrose and oligosaccharides at a ratio of 100:0, w/w), PS-B (P. persica preserved in sugar mixed with sucrose and oligosaccharides at a ratio of 60:40, w/w), PS-C (P. persica preserved in sugar mixed with sucrose and oligosaccharides at a ratio of 40:60, w/w) and PS-D (P. persica preserved in sugar mixed with sucrose and oligosaccharides at a ratio of 0:100, w/w). The results were as follows: pH levels and soluble solid contents of PS-D sample were lower than those of the others, however total acidity value were higher. In case of Hunter's value, the 'L', 'a' and 'b' values of all samples were affected by addition of oligosaccharides. No great difference occurred among samples in reducing sugar contents. Organic acid content was significantly decreased with the lower addition of sucrose. The predominating organic acid components analyzed in all samples were lactic acid 156.15 g/100 g, tartaric acid 72.75 g/100 g and citric acid 32.90 g/100 g. The highest contents of total phenol and flavonoid, and DPPH and ABTS radical scavenging activity were $10.79{\pm}0.55mg\;GAE/mL$, $8.71{\pm}0.06mg\;CE/mL$ and 71.8%, 71.0% for PS-D, respectively. The results of this study indicated the use of oligosaccharides could increase the quality and antioxidant potentials of P. persica preserved in sugar.

• 한국식품영양학회지
• /
• 제28권4호
• /
• pp.643-649
• /
• 2015

This study was performed to determine the optimal month of harvest between May and July for wild peach (Prunus davidiana) by comparison of functional materials. The general composition, amino acid and vitamin (A, C, and E) content, and antioxidant activity were determined. With respect to the general composition of wild peach according to the month of harvest, wild peach harvested in May (HMP) and July (HJP) contained mostly carbohydrate, the contents of which amounted to 77.37 g/100 g and 77.92 g/100 g, respectively. The total amino acid content of HMP and HJP were 675.29 mg% and 573.25 mg%, respectively. Regarding the vitamin contents, vitamin A (retinol), E (${\alpha}-tocopherol$) and C in HMP were higher than those in HJP. The total polyphenol content of HMP (218.37 mg TAE/g) was higher than that of HJP (71.45 mg TAE/g). The total flavonoid content of HMP (64.95 mg RE/g) was also higher than that of HJP (32.67 mg RE/g). Moreover, the DPPH and ABTS radical scavenging activities ($IC_{50}$) were higher in HMP ($137.18{\mu}g/mL$ and $157.15{\mu}g/mL$) than those in HJP ($160.10 {\mu}g/mL$ and $186.01 {\mu}g/mL$), meaning that the former produces better antioxidant effects. It can be concluded that wild peach harvested in May contained higher functional materials such as vitamins and amino acids and had a higher antioxidant effect, than wild peach harvested in july.

• 한국가구학회지
• /
• 제12권1호
• /
• pp.11-20
• /
• 2001

To investigate weathering and surface characteristics of coating film on lumbers coated with Hwangchil(varnish of Dendropanax morbifera), we carried out to investigate the resistance against discoloration, value of crack and surface characteristics of coating films on seven domestic lumbers and those of four introduced tropical lumbers. There was investigated to evaluate the properties of coating films by the varnish of Dendropanax morbifera. There was insignificant correlations between the hardness and moisture contents of each lumber treated by the varnish. There was no significant correlations between the thickness of coating film and the hardness, either. Although we investigated 11 wood species, we found only three species as resistance wood species against discoloration; Diospysos spp., Abies holophylla and Ulmus davidiana. The crack of coaing films was the largest on Zelkova serrata followed by Juglans sinensis, Cercidiphum japonicum, Tectona grands, Ulmus davidiana, Guibourtia tessmannii, Diospysos spp. and Dalbergia latifolia. However, there was no crack of coating films on the lumbers of Juniperus rigida, Prunus serrulata var. spontanea and Abies holophylla. The crack of coating film showed positive correlations with the thickness of coating film and hardness. The surface characteristics of the coating films evaluated on the basis of $R_{a}$ values indicated the lumbers of Ulmus davidiana($0.14{\;}{\mu}m$), Juniperus rigida($0.1 5{\;}{\mu}m$), Guibourtia tessmannii($0.19{\;}{\mu}m$), Dalvergia latifolia($0.20{\;}{\mu}m$) and Diospysos spp.($0.20{\;}{\mu}m$), Cercidiphum japonicum($0.24{\;}{\mu}m$) as good species while there was insignificant differences between softwoods and hardwoods. Tectona grands, Prunus serrulata var. spontanea and Abies holophylla showed rough surface with $1.53~2.63{\;}{\mu}m$ of $R_a$ values. The four kinds of rough indices($R_a,{\;}R_z,{\;}R_{max}{\;}and{\;}R_q$) showed very low correlations with moisture contents with the correlation coefficients. The $R_a{\;}and{\;}R_{max}$ also showed slight relationship with hardness.

• 생명과학회지
• /
• 제16권7호
• /
• pp.1071-1079
• /
• 2006

야생 돌복숭아(Prunus persica Batsch var. davidiana Max.)의 생리활성 추출물질이 선천성 고혈압 흰쥐(SHR)의 지질대사 이상 및 고혈압 예방과 개선효과 등에 생리생화학적 효능이 있을 것으로 추정되어 Wistar 계 수컷 SHR을 사용하여, 기본식이와 물만을 급여한 대조군인 Control군과 SHR에 돌복숭아 5.0g% 추출액을 급여한 군(5g% Ex.군) 및 SHR에 돌복숭아 10.0g% 추출액을 섭취시킨 10g% Ex.군을 33일간 실험 사육하여 혈청 지질성분 및 혈압 저하 효과를 생리적 측면에서 검토하기 위하여 본 실험을 수행하였다. 혈청 중의 총 콜레스테롤 농도, 중성지방, LDL-콜레스테롤, 유리 콜레스테롤 및 동맥경화지수 등에서 돌복숭아 생리활성물질 5.0g%, 10.0g% 추출액을 섭취시킴으로서 유의적인 농도 등의 감소 효과를 보였으며, HDL-콜레스테롤 농도 및 총 콜레스테롤에 대한 HDL-콜레스테롤 비는 상승되는 것으로 나타났다. 한편, 간장 및 뇌 중의 총 콜레스테롤, 중성지방 농도는 돌복숭아 5.0g%, 10.0g% 추출액을 섭취시킨 군에서 감소되는 것으로 나타났다. 반면, 혈청 apolipoprotein(Apo) A-I, Apo A-II 농도 등은 돌복숭아 5.0g%, 10.0g% 추출액을 섭취시킨 군에서 유의적으로 증가되는 것으로 나타났다. 또한 Apo C-II, Apo C-III, Apo E 및 Apo A-I에 대한 Apo B의 농도 비는 감소되었으며, 혈압의 변화는 SHR에 돌복숭아 5.0g% 및 10.0g% 추출액을 섭취시킴으로서 혈압이 저하됨을 관찰할 수가 있었다. 돌복숭아 5.0g% 농도(5g% Ex.군)와 10.0g% 농도(10g% Ex.군)의 추출액 섭취에 따른 두 군간에 각종 지질성분과 apolipoprotein 분획 농도 및 혈압 변화의 비교는 유의적인 차이는 없었다 따라서 야생 돌복숭아 중의 생리활성물질 추출액 섭취가 선천성 고혈압 흰쥐에 대한 혈청 지질개선 및 심장순환기계 질환, 고혈압의 예방과 치료개선에 효과가 있을 것으로 생각된다.