• Bulletin of the Korean Chemical Society
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• 제34권10호
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• pp.2905-2908
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• 2013

Fluorescent and colorimetric recognition properties of 2-(4-N-phenyl-3-thiosemicarbazone)-8-hydroxyquinoline (1) in buffered aqueous solution (1% DMSO, HEPES 20 mM, pH = 7.4) have been examined. Sensor 1 displays highly selective and sensitive recognition to $Cu^{2+}$ with fluorescence "ON-OFF" performance. The in situ formed 1-$Cu^{2+}$ complex exhibits an excellent selectivity toward sulfide ions with fluorescence "OFF-ON" behavior via $Cu^{2+}$ displacement approach. Thus, relay recognition of $Cu^{2+}$ and sulfide by a known molecule 1 has been achieved.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2011년도 추계학술논문집 1부
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• pp.295-298
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• 2011

인간의 능력 중 가장 주목할 만한 것은 언어를 습득하고 그것을 이용하여 서로 의사소통을 할 수 있다는 것이다. 모든 언어에는 그 언어만이 가지는 특수성뿐만 아니라 공통적으로 존재하는 보편적인 특성이 있다. 이것 외에도 언어 위에 입혀지는 사람의 목소리는 의사소통을 하는데 있어 상대의 심리를 파악하는 중요한 단서가 된다. 특히, 언어는 습득되어져야 활용되고 그 습득되어지는 환경에 영향을 받으며 이러한 환경에 따라 사람의 목소리, 억양 등이 변화하게 되는 것이다. 따라서 본 논문에서는 음성신호 분석 기법을 적용하여 장르별 영화시청에 따른 시각적, 청각적 요인이 목소리에 미치는 영향을 분석하는 연구를 수행하였다. 이를 위해 장르별 영화를 시청한 후 성대 진동 및 음성에너지의 크기 변화를 측정하여 감정변화를 분석하는 실험을 수행하였다.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2010년도 춘계학술발표대회
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• pp.494-497
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• 2010

본 논문에서는 $C_2H_5OH$를 주성분으로 하고 있는 알코올량에 따른 음성학적 분석 요소값의 변화를 측정하였다. 이를 위해 알코올 섭취량의 변화를 측정하고 다양한 음성 분석기법을 적용한 실험 과정을 수행하였다. 따라서 본 연구의 결과를 토대로 다양한 환경에 용이하게 쓰일 수 있도록 알코올 섭취와 관련된 분야에 적용 가능한 실험 절차를 수행하였다. 따라서 본 논문에서는 음성 분석 요소의 Pitch값과 Shimmer 및 Jitter에 대해 분석을 수행하였으며 실험 결과를 통해 알코올 섭취량이 음성에 미치는 영향을 분석하였다. 최종적으로 실험에 의해 제안한 방법의 유용성을 입증하였다.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2010년도 춘계학술발표대회
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• pp.490-493
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• 2010

오늘날 사회생활을 하는데 있어 대인관계는 원만한 조직의 운영에 필수적인 요소가 되고 있다. 그러나, 상대방의 성격을 잘 알지 못하거나 잘못 파악하여 실수하는 경우가 종종 일어난다. 이러한 문제를 해결하기 위해 본 논문에서는 영상공학적 기술을 이용하여 손톱 모양 및 크기에 따라 개인마다 보유하고 있는 성격의 특징을 연구하였다. 이를 위해 입력 영상에서 이진화 작업을 통해 1차 영상을 수집하고 필터링 및 스캐닝 작업을 통해 최종적으로 손톱의 영역을 추출하고 크기를 측정하는 방법을 사용하였다. 이를 기반으로 결과 영상 및 손톱의 수치값을 측정하고 손톱의 크기를 비교, 분석하였다. 결과적으로 실험값을 통해 손톱의 형태 및 크기를 비교, 분석하였으며 손톱의 크기에 따른 개인의 성격에 대해 분석하였다.

• Parasites, Hosts and Diseases
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• 제62권1호
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• pp.75-84
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• 2024

Ancylostoma ceylanicum is a zoonotic soil-derived nematode that parasitizes the intestines of humans and animals (dogs and cats), leading to malnutrition and iron-deficiency anemia. Helminth parasites secrete calreticulin (CRT), which regulates or blocks the host's immune response. However, no data on A. ceylanicum calreticulin (Ace-CRT) are available. We investigated the biological function of recombinant Ace-CRT (rAce-CRT). rAce-CRT showed reliable antigenicity and stimulated the proliferation of mouse splenocytes and canine peripheral blood mononuclear cells. Quantitative reverse-transcription PCR assays revealed that rAce-CRT primarily promoted the expression of T helper 2 cytokines, particularly IL-13, in canine peripheral blood lymphocytes. rAce-CRT inhibited complement-mediated sheep erythrocyte hemolysis in vitro. Our findings indicate that Ace-CRT plays an immunomodulatory role and may be a promising candidate molecule for a hookworm vaccine.

• Asian-Australasian Journal of Animal Sciences
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• 제32권7호
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• pp.922-929
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• 2019

Objective: Mutations in low-density lipoprotein receptor (LDLR), which encodes a critical protein for cholesterol homeostasis and lipid metabolism in mammals, are involved in cardiometabolic diseases, such as familial hypercholesterolemia in pigs. Whereas microRNAs (miRNAs) can control LDLR regulation, their involvement in circulating cholesterol and lipid levels with respect to cardiometabolic diseases in pigs is unclear. We aimed to identify and analyze LDLR as a potential target gene of SSC-miR-20a. Methods: Bioinformatic analysis predicted that porcine LDLR is a target of SSC-miR-20a. Wild-type and mutant LDLR 3'-untranslated region (UTR) fragments were generated by polymerase chain reaction (PCR) and cloned into the pGL3-Control vector to construct pGL3 Control LDLR wild-3'-UTR and pGL3 Control LDLR mutant-3'-UTR recombinant plasmids, respectively. An miR-20a expression plasmid was constructed by inserting the porcine premiR-20a-coding sequence between the HindIII and BamHI sites in pMR-mCherry, and constructs were confirmed by sequencing. HEK293T cells were co-transfected with the miR-20a expression or pMR-mCherry control plasmids and constructs harboring the corresponding 3'-UTR, and relative luciferase activity was determined. The relative expression levels of miR-20a and LDLR mRNA and their correlation in terms of expression levels in porcine liver tissue were analyzed using reverse-transcription quantitative PCR. Results: Gel electrophoresis and sequencing showed that target gene fragments were successfully cloned, and the three recombinant vectors were successfully constructed. Compared to pMR-mCherry, the miR-20a expression vector significantly inhibited wild-type LDLR3'-UTR-driven (p<0.01), but not mutant LDLR-3'-UTR-driven (p>0.05), luciferase reporter activity. Further, miR-20a and LDLR were expressed at relatively high levels in porcine liver tissues. Pearson correlation analysis revealed that porcine liver miR-20a and LDLR levels were significantly negatively correlated (r = -0.656, p<0.05). Conclusion: LDLR is a potential target of miR-20a, which might directly bind the LDLR 3'-UTR to post-transcriptionally inhibit expression. These results have implications in understanding the pathogenesis and progression of porcine cardiovascular diseases.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2013년도 춘계학술발표대회
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• pp.782-784
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• 2013

스마트 자동차, 선박 및 공장 자동화와 같은 산업 분야에서 이더넷 기반 산업 네트워크의 연구가 추진되고 있다. IEEE802.1AVB와 같은 실시간 이더넷 프로토콜을 살펴보고 산업용 스위치드 이더넷 기반 통신 프로세서에서 우선 순위 트래픽을 처리하는 큐 모델링과 성능 분석을 수행한다.

• Journal of Ginseng Research
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• 제47권2호
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• pp.173-182
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• 2023

Cancer is a global public health issue that becomes the second primary cause of death globally. Considering the side effects of radio- or chemo-therapy, natural phytochemicals are promising alternatives for therapeutic interventions to alleviate the side effects and complications. Ginsenoside Rh2 (GRh2) is the main phytochemical extracted from Panax ginseng C.A. Meyer with anticancer activity. GRh2 could induce apoptosis and autophagy of cancer cells and inhibit proliferation, metastasis, invasion, and angiogenesis in vitro and in vivo. In addition, GRh2 could be used as an adjuvant to chemotherapeutics to enhance the anticancer effect and reverse the adverse effects. Here we summarized the understanding of the molecular mechanisms underlying the anticancer effects of GRh2 and proposed future directions to promote the development and application of GRh2.

• Asian-Australasian Journal of Animal Sciences
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• 제15권10호
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• pp.1391-1394
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• 2002

The polymorphism of insulin-like growth factor-I (IGF-I) in 13 pig breeds (total n=559) was detected by PCR-Hha I- RFLP, and allele A (151 bp and 28 bp) or allele B (116 bp, 35 bp and 28 bp) were observed. In these pig breeds, it was found that European pig breeds carried high frequencies of allele B, while Chinese native pig breeds carried high frequencies of allele A. Meanwhile the role of porcine IGF-I was investigated in 117 Nanchang White pigs and 360 Large Yorkshire pigs. Eight traits about growth and carcass were recorded for analyzing the associations between IGF-I gene polymorphism and performance quantitative traits. In the Nanchang White pigs, those with AA genotype generally had higher birth weight than those with AB genotype (p<0.05), but all these genotypes had no significant effect on the other traits which had been analyzed. In Large Yorkshire pigs, those with BB genotype had higher 2 months and 6 months body weight than those with AA genotype (p<0.05), and had a thicker hind-back-fat thickness and mid-back-thickness than those with AB and BB genotypes (p<0.05). And those with BB genotype were the thinnest in Large Yorkshire. Furthermore, pigs with AA genotype had a lower lean percentage than those with AB and BB genotypes (p<0.01), and the lean percentage of those with BB genotype was the highest. Based on these results, it is possible to make the IGF-I gene locus into the application of marker-assisted selection programmes.

• Asian Pacific Journal of Cancer Prevention
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• 제13권10호
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• pp.5161-5165
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• 2012

Background: There are few molecular markers known to predict cholangiocarcinoma (CCA) prognosis. Smad7 has a certain relationship with epithelial-mesenchymal transition (EMT), but its relevance to CCA in unclear. Therefore expression and clinical significance of Smad7 in CCA was the focus of this study. Methods: Expression of Smad7, E-cadherin and vimentin was assessed in 41 patients with CCA by immunohistochemistry and analyzed for associations with clinical parameters. Results: Smad7 and vimentin expression in the CCA tissue was dramatically higher than that in adjacent tissues. In addition, Smad7, vimentin and E-cadherin expression was significantly associated with CCA lymph node metastasis and perineural invasion($P{\leq}0.05$), but not other factors, such as gender, age, tumor location, tumor type and tumor differentiation degree (P>0.05). The overall survival and relapse-free survival rate was significantly higher in patients with negative Smad7 expression than those with positive Smad7 expression. Conclusion: EMT phenomena may occur in the process of CCA invasion and metastasis. Smad7, which was highly expressed in CCA, may be considered to be one feedback regulator in late stages and could have potential as a prognostic indicator for clinical assessment.