• 한국인터넷방송통신학회논문지
• /
• 제15권6호
• /
• pp.123-128
• /
• 2015

최근 IoT(Internet of Things)에서 다양한 사물을 인터넷에 연결하여 정보를 교환하는 통신 프로토콜에 대한 관심이 집중되고 있다. 현재 IETF(Internet Engineering Task Force) 표준화 기구에서는 제한된 환경에서 센서나 구동체와 같은 사물 간의 통신을 지원하는 CoAP(Constrained Application Protocol) 프로토콜을 표준으로 채택하고 있다. 향후 IoT 환경에서 서버뿐만 아니라 스마트 폰에서 센서로 부터 데이터를 수집하거나, 구동체에게 명령을 전달하고, 사물을 관리하는 역할을 담당할 것으로 예상된다. 본 논문에서는 IETF CoAP 을 기반으로 스마트 폰과 IoT 노드사이의 연결 구성을 설계하고, 스마트 폰에서 데이터 정제 기능을 갖는 미들웨어를 설계하고 구현한다. 제안된 데이터 정제 기법을 이용하여 송수신 과정에서 발생하는 오류 데이터와 중복 데이터를 확인하고 제거함으로써 제한된 외부 환경에서 신뢰성 있는 정보를 전달하는 데 기여할 것이다.

• 한국자기공명학회논문지
• /
• 제26권1호
• /
• pp.1-9
• /
• 2022

There are two distinct approaches to improving the quality of protein NMR structures during refinement: all-atom force fields and accumulated knowledge-assisted methods that include Rosetta. Mao et al. reported that, for 40 proteins, Rosetta increased the accuracies of their NMR-determined structures with respect to the X-ray crystal structures (Mao et al., J. Am. Chem. Soc. 136, 1893 (2014)). In this study, we calculated 32 structures of those studied by Mao et al. using all-atom force field and implicit solvent model, and we compared the results with those obtained from Rosetta. For a single protein, using only the experimental NOE-derived distances and backbone torsion angle restraints, 20 of the lowest energy structures were extracted as an ensemble from 100 generated structures. Restrained simulated annealing by molecular dynamics simulation searched conformational spaces with a total time step of 1-ns. The use of GPU-accelerated AMBER code allowed the calculations to be completed in hours using a single GPU computer-even for proteins larger than 20 kDa. Remarkably, statistical analyses indicated that the structures determined in this way showed overall higher accuracies to their X-ray structures compared to those refined by Rosetta (p-value < 0.01). Our data demonstrate the capability of sophisticated atomistic force fields in refining NMR structures, particularly when they are coupled with the latest GPU-based calculations. The straightforwardness of the protocol allows its use to be extended to all NMR structures.

• BMB Reports
• /
• 제46권7호
• /
• pp.335-337
• /
• 2013

Scientists planning research that involves the use of animals are required by international and/or national law to examine the possibilities for the implementation of Replacement, Reduction and/or Refinement (the Three Rs principles of Russell and Burch) in experiments for research, testing, and education. There are two Korean laws legislating humane use of animals and ethical review prior to animal experiments. This report reviews current practice of the literature search by the researchers and protocol review by the Institutional Animal Care and Use Committees on the Replacement, Reduction and Refinement alternatives in Korea. The promotion and protection of the laboratory animals are one of the core competencies of investigators exploring the ethical conduct of research and good science.

• Journal of Communications and Networks
• /
• 제7권1호
• /
• pp.45-53
• /
• 2005

In this paper, we present a cross-layer analysis of wireless TCP systems over correlated channels. The effects of error correlation on the behavior of link retransmission strategy and the end-to-end throughput of TCP layer are investigated. Based on the cross-layer analysis, an efficient refinement of link layer protocol is proposed by consciously utilizing the information of channel correlations, which leads to the performance improvement of wireless TCP systems.

• 한국통신학회논문지
• /
• 제27권4A호
• /
• pp.310-315
• /
• 2002

본 논문은 객관적 음질 평가법으로 웨이블렛 변환을 이용한 향상된 바크 코히어런스 함수 (Wavelet based Bark Coherence Function : WBCF)를 제안한다. 바크 코히어런스 함수 (Bark Coherence Function : BCF)는 심리 음향 영역에서 코히어런스 함수를 정의함으로서 음성 통신 시스템의 아날로그 부분에 의하여 발생할 수 있는 선형 왜곡에 강한 객관적 음질 평가법이다. VoIP (Voice over Internet Protocol)와 같은 패킷 기반의 음성 전달 시스템은 가변 지연등이 발생 될 수 있는데, 이것은 원음과 왜곡음의 정확한 시간축 정렬을 불가능하게 하여 기존의 객관적 음질 평가법의 성능을 저하시킨다. 제안된 WBCF는 고주파 영역에서 시간 분해능이 높으며, 저주파 영역에서 주파수 분해능이 높은 웨이블렛 변환을 사용한 후 BCF를 계산하여 VoIP 시스템에서의 객관적 음질을 평가한다. 주/객관적 음질 평가 실험을 통하여 WBCF가 ITU-T 권고안인 Perceptual Speech Quality Measure (PSQM)에 비하여 높은 성능을 가짐을 확인하였다.

• Biomolecules & Therapeutics
• /
• 제23권4호
• /
• pp.379-385
• /
• 2015

The eye irritation potential of drug candidates or pharmaceutical ingredients should be evaluated if there is a possibility of ocular exposure. Traditionally, the ocular irritation has been evaluated by the rabbit Draize test. However, rabbit eyes are more sensitive to irritants than human eyes, therefore substantial level of false positives are unavoidable. To resolve this species difference, several three-dimensional human corneal epithelial (HCE) models have been developed as alternative eye irritation test methods. Recently, we introduced a new HCE model, MCTT HCE$^{TM}$ which is reconstructed with non-transformed human corneal cells from limbal tissues. Here, we examined if MCTT HCE$^{TM}$ can be employed to evaluate eye irritation potential of solid substances. Through optimization of washing method and exposure time, treatment time was established as 10 min and washing procedure was set up as 4 times of washing with 10 mL of PBS and shaking in 30 mL of PBS in a beaker. With the established eye irritation test protocol, 11 solid substances (5 non-irritants, 6 irritants) were evaluated which demonstrated an excellent predictive capacity (100% accuracy, 100% specificity and 100% sensitivity). We also compared the performance of our test method with rabbit Draize test results and in vitro cytotoxicity test with 2D human corneal epithelial cell lines.

• BMB Reports
• /
• 제50권12호
• /
• pp.610-614
• /
• 2017

Scientists planning research that involves the use of animals are required to examine the possibilities for replacement, reduction, or refinement (the Three Rs), and their protocol must be reviewed by the Institutional Animal Care and Use Committee. Good scientific practice involving appropriate search techniques on the available Three Rs resources is essential for both ethical and scientific reasons. Appropriate experimental design and statistical analysis techniques are particularly necessary for research involving the use of animals, because this can improve animal welfare and scientific outcomes, as well as saving animal lives. There are a number of resources to help researchers improve their search techniques, experimental design strategies, and their reporting of research involving the use of animals. However, there is little specific information or resources on the Three Rs alternatives that is readily available in the Korean language. This paper outlines the common errors made by submitting researchers that have been repeatedly observed during the ethical review of experimental protocols over the last ten years, and provides information on the Korean resources available to promote good scientific practice. This could help to bridge the gap between Korean scientists and animal welfare advocates assisting scientists to improve ethical considerations and conduct responsible research.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권2호
• /
• pp.1101-1106
• /
• 2013

Background: Osteosarcoma is the most common primary bone tumor in childhood and adolescence. Carboplatin, a platinum-derived agent, is used as neoadjuvant chemotherapy for pediatric osteosarcoma because of its anti-tumor activity and had low toxicity as compared to cisplatin. Objective: To determine demographic data, prognostic factors and outcome of childhood osteosarcoma treated with a carboplatin-based chemotherapeutic protocol at Chiang Mai University. Method: A retrospective analysis was conducted on 34 osteosarcoma patients aged less than 18 years and treated between 2003 and 2011. Results: Overall limb-salvage and amputation rates were 23.5% and 70.6%, respectively. With the mean follow-up time of 29.5 months (1.5-108.9), the Kaplan-Meier analysis for 3-year disease-free survival (DFS) and 3-year overall survival (OS) were $20.2{\pm}7.7%$ and $47.1{\pm}9.5%$ respectively. Patients who had initial pulmonary metastasis were at significantly greater risk for developing recurrence (p=0.02, OR=7; 1.2-40.1) and had a tendency to have lower 3-year OS compared to those without initial pulmonary metastasis ($28.1{\pm}13%$, $63.1{\pm}12.3%$, respectively, p=0.202). On univariate analysis, age at diagnosis >14 years and patients who were declined surgery were significantly associated with lower 3-year OS (p=0.008 and <0.05, respectively). However, age at diagnosis, sex, tumor size and histological subtypes were not found to significantly affect recurrence or survival. Conclusions: In our study, the survival rate was far lower than those reported from developed countries. These might indicate the ineffectiveness of carboplatin in combination with doxorubicin as frontline treatment of pediatric osteosarcoma, especially in those with initial pulmonary metastasis. Refinement in risk and treatment stratification and dose intensification for pediatric osteosarcoma constitutes a future challenge to improve outcomes, especially in metastatic patients who may need a more intensive regimen.

• Archives of Plastic Surgery
• /
• 제38권5호
• /
• pp.642-648
• /
• 2011

Purpose: The recent advances in microsurgical techniques and their refinement over the past decade have greatly expanded the indications for digital replantations and have enabled us to salvage severed fingers more often. Many studies have reported greater than 80% viability rates in replantation surgery with functional results. However, replantation of multi-level amputations still remain a challenging problem and the decision of whether or not to replant an amputated part is difficult even for an experienced reconstructive surgeon because the ultimate functional result is unpredictable. Methods: Between January of 2002 and May of 2008, we treated 10 multi-level amputated digits of 7 patients. After brachial plexus block, meticulous replantation procedure was performed under microscopic magnification. Postoperatively, hand elevation, heat lamp, drug therapy and hyperbaric oxygen therapy were applied with careful observation of digital circulation. Early rehabilitation protocol was performed for functional improvement. Results: Among the 19 amputated segments of 10 digits, 16 segments survived completely without any complications. Overall survival rate was 84%. Complete necrosis of one finger tip segment and partial necrosis of two distal amputated segments developed and subsequent surgical interventions such as groin flap, local advancement flap and skin graft were performed. The overall result was functionally and aesthetically satisfactory. Conclusion: We experienced successful replantations of multi-level amputated digits. When we encounter a multi-level amputation, the key question is whether or not it is a contraindication to replantation. Despite the demand for skillful microsurgical technique and longer operative time, the authors' results prove it is worth attempting replantations in multi-level amputation because of the superiority in aesthetic and functional results.