• 제목/요약/키워드: Protein-fiber

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Interactions between Chicken Salt-soluble Meat Proteins and Makgeolli Lees Fiber in Heat-induced Gels

  • Choi, Yun-Sang;Park, Kwoan-Sik;Kim, Hack-Youn;Kim, Hyun-Wook;Song, Dong-Heon;Chung, Hai-Jung;Lee, Ju-Woon;Kim, Cheon-Jei
    • 한국축산식품학회지
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    • 제31권6호
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    • pp.817-826
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    • 2011
  • The technological effects of Makgeolli lees fiber (0, 0.5, 1.0, 2.0, and 4.0%) on chicken salt-soluble breast meat proteins in a model system on proximate composition, physicochemical properties, and textural properties were investigated. Makgeolli lees fiber was obtained from Makgeolli brew processing, and the by-products showed good dietary fiber. The moisture and ash contents, water holding capacity, redness, yellowness, hardness, and apparent viscosity of chicken salt-soluble meat protein heat-induced gel systems with Makgeolli lees fiber were all higher than the control without Makgeolli lees fiber. However, protein solubility and electrophoretic patterns did not differ among the control and treatments with Makgeolli lees fiber samples. The chicken salt-soluble protein heat-induced gel systems incorporating Makgeolli lees fiber had improved water holding capacity, textural properties, and viscosity due to Makgeolli lees fiber addition. These results suggest that the addition of 4.0% Makgeolli lees fiber to gel is helpful to improve the physical properties of heat-induced gels.

재조합 가금 아데노바이러스 Fiber 2 단백질을 이용한 특이 난황 항체 생산 (Production of Specific Egg Yolk Antibodies in Chicken against Recombinant Fowl Adenovirus Fiber 2 Protein)

  • 정경민;이성;김정우
    • 한국가금학회지
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    • 제41권1호
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    • pp.15-20
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    • 2014
  • 본 연구는 가금에서 호흡기 질환, 심낭수종, 봉입체 간염, 산란저하증 등을 유발하는 가금 아데노바이러스의 fiber 2 유전자에 대한 특이 난황 항체를 개발하고자 실험을 실시하였다. Fiber 2 유전자를 클로닝한 뒤, 대장균 발현 시스템을 이용하여 약 22 kDa의 재조합 fiber 2 단백질을 생산하였다. 이를 산란계에 3주 간격으로 총 4회 면역하여 혈청 및 난황 내 항체가를 측정한 결과, 면역 후 12주경에 항체가가 최고치에 달하였으며, 산란계로부터 획득한 난황 항체를 이용한 Western blot analysis 결과, 가금 아데노바이러스 내 fiber 2 단백과 특이적으로 반응한다는 것을 규명하였다. 결론적으로, 가금 아데노바이러스 fiber 2에 특이적인 난황 항체 생산에 성공하였으며, 이러한 특이적인 난황 항체는 가금 아데노바이러스로 인한 질병의 예방 및 치료에 활용 가능할 것으로 사료된다.

일부 여대생의 식이섬유 섭취와 철분대사에 관한 연구 (A Study on the Dietary Fiber Intake and Iron Metabolism in Korean Female College Students)

  • 승정자
    • Journal of Nutrition and Health
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    • 제30권2호
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    • pp.147-154
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    • 1997
  • This study was conducted to evaluate dietary fiber intake, iron status, and their correlations in 50 female college students living in Seoul. The nutritional status was analyzed using 3-day dietary record, duplicated diet collection, and venous blood sampling. The mean values for age, height, weight, BMI, and blood pressure of the subjects were 23.2 years, 160.2cm, 53.9kg, 21.0kg/$m^2$, and 110.1/68.4mmHg, respectively. Daily intakes of enery, protein, fiber, crude fiber, iron, and heme iron were 1635.5㎉, 54.3g, 22.5g, 6.8g, 16.2mg, and 0.2mg, respectively. Fiver intake was positively correlate with energy, protein, carbohydrate, vitamin C, iron, and crude fiber intakes. Also, iron intake was positively correlated with energy, protein, lipid, carbohydrate, and vitamin C intakes. There was a significant correlation between heme iron and MFP(meat, fish, poultry) intakes. To exame the iron balance, iron intake and excretion were measured. Iron intake and excretions through urine and feces were 19.5mg, 8.5mg, and 1.6mg, respectively. Based on these iron retention and iron apparent absorbability were calculated as 9.4mg and 52.4%, respectively. There was no significant relationship between dietary fiber intake and iron status. However, there were significant positive correlation between fiber intake and urinary iron excretion.

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식이섬유 수준이 유색육용계의 육질에 미치는 영향 (Effect of Dietary Fiber Level on Meat Quality in Colored Broiler)

  • 김미숙;문윤희;임사비나;김대진
    • 생명과학회지
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    • 제7권4호
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    • pp.329-335
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    • 1997
  • This study was conducted to investigate the effect of dietary fiber(DF) levels on the meat quality in colored broiler. Colored broiler were fed on containing corn-soy basal diet(DF 5%) and high level(DF 6,7 and 8%) of dietary fiber diets for 7 weeks. Dietary fiber level of diet was make up by adding some alffalfa meal. Colored broiler meats were stored at 3$\circ$ for 24hr after skaughter, and used to analyze physico-chemical properties. Proximate component, pH, shear force value, myofibril fragmentation index, water holding capacity, cooking loss, protein extractability, fatty acid composition, Hunter's L, a value and palatability of cooked meat were not significantly affected by dietary fiber levels, whereas the Hunter's value of meat was significantly affected bty dietary fiber levels for the final period of feeding. Crude protein content, myofibril fragmentation index, water holding capacity, protein extractability and Hunter's b value of breast meat's were higher than thigh meat's, but crude fat content, pH, shear force value, cooking loss, palmitoleic acid, linolenic acid, and Hunter's a value were lower, regardless of dietary fiber level.

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Effects of Maternal Nutrition during Pregnancy on the Body Weight, Muscle Fiber Number, Carcass Traits, and Pork Quality Traits of Offspring

  • Choe, J.H.;Choi, Y.M.;Ryu, Y.C.;Lee, S.H.;Kim, B.C.
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권7호
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    • pp.965-971
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    • 2010
  • The purpose of the current study was to examine the influence of different maternal nutrition treatments during pregnancy on body weight, muscle fiber number, carcass traits, and pork quality traits of offspring. A total of 18 crossbred sows (Landrace${\times}$Yorkshire${\times}$Duroc) were randomly assigned to one of three nutritional treatment groups; control, high energy, and high protein. The control group was fed a standard diet, the high energy group was fed a diet that contained 30% increased metabolizable energy, and the high protein group was fed a diet that contained 30% increased limiting amino acids compared to the control. The sows in each group were fed equal quantities of each diet (1.9 kg/d) for the entire gestational period. A total of 36 piglets from each sow were used to evaluate changes in body weight, muscle fiber number in the longissimus dorsi muscle at birth, carcass traits, and pork quality traits. Birth weight of offspring born to sows in the high energy diet group was significantly higher compared to the high protein diet group (p<0.05). However, body weight of offspring after birth was not significantly different between the groups. Muscle fiber number for the longissimus dorsi muscle at birth was not significantly different between the groups. In addition, there were no significant differences in carcass traits or pork quality traits between offspring born to sows in the control group and those born to sows that received high energy or high protein diets during pregnancy. Based on these results, it appears that maternal nutrition treatment during pregnancy, regardless of whether it is with high energy or high protein diets, does not have a significant effect on body weight, muscle fiber number at birth, carcass traits, or pork quality traits.

쑥갓과 머위의 잎과 중기의 구조탄수화물의 변화 (The Changes in Structural Carbohydrate on Crown Daisy and Butterbur)

  • 김대진;윤수현;조영수;최미애
    • 생명과학회지
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    • 제9권5호
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    • pp.497-503
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    • 1999
  • Change on the structural carbohydrate(several fiberous components) was determined by vegetables(crown daisy and butterbur)-cultivated in Ulsan, Kyungnam, Korea-as its stage of maturity developed. Samples were separated into leaf and stem, which were dried at 7$0^{\circ}C$ for 24hr, and ground to pass a 1mm screen. They were subjected to moisture, crude protein, crude fat and several dietary fiber-DF(dietary fiber, include unavaible components), NDF(neutral detergent fiber), ADF(acid detergent fiber), lignin, hemicellulose, cellulose and protein corrected NDF(c-NDF), IDF(indigestible fiber, include lignin, hemicellulose and cellulose). In general, structural carbohytrate(several dietary fiber) of vegetable was affected by the growth stage. In case of crown daisy and butterbur, dietary fiber in leaf was higher than DF in stem.

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근위축 발생전의 지구력 운동이 쥐의 위축뒷다리근의 질량, 근원섬유 단백질 함량 및 근섬유 단면적에 미치는 영향 (Effect of Endurance Exercise Prior to Occurrence of Muscle Atrophy on the Mass, Myofibrillar Protein Content and Fiber Crossectional Area of Atrophied Hindlimb Muscles of Rats)

  • 최명애
    • 대한간호학회지
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    • 제27권1호
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    • pp.96-108
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    • 1997
  • The purpose of this study was to determine the effect of endurance training prior to occurrence of muscle atrophy on the mass, myofibrillar protein content and fiber crossectional area of atrophied hindlimb muscles of rats. Adult female Wistar rats were trained prior to occurrence of muscle atrophy induced by hindlimb suspension. Training began on the 1st day for 10min /day at 15m /min on a 0% grade, training exercise increased daily in time and intensity so that by the 4th week rats were running 60min /day, at 34m /min on a i3.5% grade. Wet weight and relative weight of soleus, plantaris and gastrocnemius muscle decreased significantly after seven days of hindlimb suspension. Wet weight and relative weight of soleus tended to increase and that of plantaris and gastrocnemius tended to decrease in the exercise group as compared to the control group. Myofibrillar protein content of soleus and gastrocnemius tended to increase and that of plantaris tended to decrease in the endurance trained group as compared to the control group. Fiber crossectional area of Type I, II fiber in soleus and plantaris muscle tended to increase in the exercise group as compared to the control group. Wet weight and relative weight of soleus. plantaris and gastrocnemius decreased significantly, myofibrillar protein content of soleus, plantaris and gastrocnemius increased in hindlimb suspended rats following endurance training as compared to the control group. There was no change in fiber type percentage and crossectional area of type I and II fiber in soleus muscle and that of type I and IIfiber in plantaris muscle decreased in the hindlimb suspended rats following endurance training as compared to the control group. Wet weight and relative weight of soleus and plantaris tended to increase, that of gastrocnemius increased significantly, myofibrillar protein content of soleus and plantaris muscle increased significantly and that of gastrocnemius tended to increase in the hindlimb suspended rats following endurance training as compared to sedentary rats following endurance training. Crossectional area of type I fiber of soleus muscle tended to increase. that of type I fiber of plantaris muscle increased significantly and that of type II fiber tended to increase in hindlimb suspended rats following endurance training as compared to sedentary rats following endurance training. The results suggest that endurance training prior to occurrence of muscle atrophy can attenuate the decrease of mass, myofibrillar protein content and fiber crossectional area induced by hindlimb suspension.

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Quality properties of various dietary fibers as isolated soy protein (ISP) replacements in pork emulsion systems

  • Park, Sin-Young;Oh, Tae-Seok;Kim, Gye-Woong;Kim, Hack-Youn
    • Journal of Animal Science and Technology
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    • 제62권1호
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    • pp.94-102
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    • 2020
  • This study aimed to investigate the possibility of replacing the isolated soy protein (ISP) as a binding agent for wheat, oat, and bamboo shoot dietary fibers. Dietary fibers and ISP were added to manufacturing process of pork emulsion, respectively, for investigate quality properties. Moisture contents of pork emulsion added wheat fiber-treated group was significantly higher than ISP-treated group (p < 0.05), and protein contents of dietary fiber-treated group were significantly lower than ISP-treated group (p < 0.05). Raw pork emulsion CIE a value of oat, bamboo shoot fiber-treated group were significantly lower than ISP-treated group (p < 0.05). After cooking pork emulsion CIE L value of dietary fiber-treated group were significantly higher than ISP-treated group (p < 0.05). Raw pork emulsion water holding capacity (WHC) of wheat, oat fiber-treated group were significantly higher than ISP-treated group (p < 0.05), and cooked pork emulsion WHC of wheat, bamboo shoot fiber-treated group were higher than ISP-treated group (p < 0.05). Cooking loss of ISP-treated group was significantly higher than dietary fiber-treated group (p < 0.05), and viscosity of ISP-treated group was lower than dietary fiber-treated group. Hardness of ISP-treated group was significantly lower than dietary fiber-treated group (p < 0.05); however, cohesiveness of ISP-treated group was significantly higher than dietary fiber-treated group (p < 0.05). In conclusion, dietary fiber added as binding agent to manufacturing process of pork emulsion was suitable to replacing ISP.

식이 섬유소가 어류단백 소화율에 미치는 영향 (Effect of Dietary Fiber on the In Vitro Digestibility of Fish Protein)

  • Ryu, Hong-Soo;Park, Nam-Eun;Lee, Kang-Ho
    • 한국식품영양과학회지
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    • 제21권3호
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    • pp.255-262
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    • 1992
  • 단백소화율에 미치는 식이 섬유소의 영향에 대하여 알아보기 위해, 채소류(상치, 깻잎, 고추. 다시마)로부터 추출한 식이 섬유소와 시판용 정제 식이 섬유소 (cellulose, pectin, sodium alginate, gum karaya)를 어류 단백질인 말쥐치 단백질(냉동건조육 및 myofibrils)에 첨가 반응시켜, 단백질 의 소화율에 어느 정도 영향을 미치는가에 대해 알아보았다. 각 시료의 중성세제 추출섬유소 (neutral detergent fiber) 함량은 24.21%(고추) 9.75%(다시마)의 범위였고, 산성세제 추출섬유소 (acid detergent fiber) 함량은 20.85%(고추) 11.97%(깻잎)의 범위였으며, 수용성 섬유소 함량은 13.79%(다시마) 4.41%(상치)의 범위였다. 말쥐치 단백질에 대한 식이 섬유소의 반응 비율을 1 : 1 (wt/wt)로 하고, 37$^{\circ}C$에서 2시간 동안 반응시켰을 때. 말쥐치 단백소화율은 정제 식이 섬유소 첨가의 경우, 1.52%(cellulose) 9.97%(pectin)가 감소되었고. 추출한 식이 섬유소 첨가의 경우, 5.15%(고추) 12.36%(다시마)가 감소되었다. 섬유소의 trypsin 활성저해능은 단백소화율이 감소함에 따라 증가하여, ANRC casein에 대한 soybean trypsin inhibitor 22mg/g (cellulose) 61.82mg/g(gum karaya), 49.75mg/g(고추) 171.52mg/g(상치)에 상응하는 것으로 나타났다. 정제 식이 섬유소에 의한 단백분해효소의 활성 변화는 sodium alginate를 제외하고는 거의 없어, 어류 단백소화율의 저하는 식이 섬유소가 단백질에 직접 결합하여 비소화성 물질을 형성한 결과가 주도하리라 생각되었다. 말쥐치 단백질과 섬유소를 반응시킨 것을 효소 가수분해시킨 후에 측정한 유리 필수 아미노산의 함량은 sodium alginate와 다시마 섬유소의 경우 현저하게 저하하였으며(75% 이상), isoleucine과 valine이 크게 영향을 받았다.

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저강도 격일 운동과 매일 운동이 쥐 뒷다리근에 미치는 근 비대 효과 비교 (Comparison of Hypertrophic Effects of Low-Intensity Exercise on Rat Hindlimb Muscles between Every Other Day Exercise and Everyday Exercise)

  • 최명애;고종진;곽현경;백지현;정진영;송연정;안경주
    • Journal of Korean Biological Nursing Science
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    • 제13권1호
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    • pp.1-7
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    • 2011
  • Purpose: The purpose of this study was to compare the hypertrophic effects of low-intensity exercise on weight, myofibrillar protein content and Type I, II fiber cross-sectional area of hindlimb muscles of rats between every other day exercise and every day exercise. Methods: Adult male Sprague-Dawley rats were assigned to 1 of 3 groups: control group (C, n=6), experimental group 1 (E1, n=7) and experimental group 2 (E2, n=7). Rats in E1 group had 7 sessions (every other day) and those in E2 group had 14 sessions (every day) of exercise in which they ran on a treadmill for 30 min/day at 10 m/min. Results: Muscle weight, cross-sectional area of type I fiber and myofibrillar protein content of soleus and myofibrillar protein content of plantaris in E1 group, and myofibrillar protein content of soleus and cross-sectional area of type I fiber of plantaris in E2 group were greater than those in C group. Cross-sectional area of type I fiber of soleus of E1 group was higher than E2 group while cross-sectional area of type I fiber of plantaris of E2 group was higher than E1 group. Conclusion: Hypertrophy of hindlimb muscles occurs from every other day exercise similar to every day exercise.