• Journal of Nutrition and Health
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• 제57권2호
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• pp.261-274
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• 2024

Purpose: Recently, high-protein diets have become highly popular, and the market for protein products has steadily increased in Korea together with the development of various types of such products. However, there is limited information on the consumption of protein supplements (PS) or protein-fortified foods (PF). Thus, this study aimed to evaluate the use of PS/PF among young adults in Jeju. Methods: A total of 350 adults (140 men and 210 women) aged 19-39 years voluntarily participated in this study from June 2022 to May 2023. PS/PF use was measured using a questionnaire. Dietary intake was assessed using a 24-hour dietary recall. Results: Approximately 31.4% of the participants (n = 110) had consumed PS/PF for more than 2 weeks during the past year and 71.8% of them (n = 79) were still consuming these products (PS/PF consumers). The PS/PF consumers tended to be male and physically active (p < 0.05 for all). The most frequent reason for PS/PF use was muscle gain (59.5%), followed by protein supplementation (19.0%) and body fat loss (13.9%), and the most frequent type of PS/PF consumed was powders (70.6%), followed by drinks (17.7%) and bars (8.8%). The PS/PF consumers tended to consume a high-protein low-carbohydrate diet compared to the non-consumers. The prevalence of consuming dietary protein less than the estimated average requirement (EAR) was significantly lower in PS/PF consumers (13.9%) compared to non-consumers (25.4%; p = 0.0316). Conclusion: These findings indicate that the necessity of protein supplementation should be determined based on the current dietary protein intake and individual requirements. The study also provides the basic information for establishing guidelines for appropriate protein intake.

• 식품과학과 산업
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• 제52권4호
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• pp.387-400
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• 2019

Fat replacers are divided into three categories. These include carbohydrate-based, protein-based and fat-based replacers. Carbohydrate-based replacers occupy half of the fat replacers market. The main ingredients of carbohydrate-based are gums, starch, modified starch, cellulose and fiber. The functional properties of fat replacers are to retain moisture, to retard staling, to provide mouthfeel and texture, to emulsify, to stabilize emulsion, and to reduce fat. Using these functionalities, fat replacers are used in various foods such as baked goods, salad dressing, sauces, meat products, dairy products, frying foods, bakery, and confectionery. Success factors of fat replacers in the market are sensory equivalent, texture and safety as food ingredients.

• Journal of Pharmaceutical Investigation
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• 제21권1호
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• pp.43-50
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• 1991

The hepatic uptake of an anionic fluorescence probe, l-anilino-8-naphthalene sulfonate (ANS) was characterized using isolated rat hepatocytes. The initial uptake rate of ANS by isolated hepatocytes was determined. The uptake process of ANS was fitted well to the Michaelis-Menten equation with a saturable component. The $V_{max}$ and $K_m$ values were $2.9{\pm}0.1\;nmol/min/mg$ protein and $29.1{\pm}3.2\;{\mu}M$, respectively. The uptake clearance $(CL_{up})$ based on the ratio of $V_{max}$ to $K_m$ was 11.7 ml/min/g liver, revealing the good coincidence with that assessed from the analysis of the plasma disappearance curve in previous report. Furthermore, the effect of serum protein on the hepatic uptake of ANS into isolated hepatocytes was investigated. The permeability clearances $(PS_{inf})$ of ANS uptake were much higher than those predicted based on the unbound fractions in the presence of serum. These suggested that the hepatic uptake of extensively serum protein-bound ANS is mediated not only by the unbound form of ligand but also by the serum protein-mediated uptake mechanism.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.620-627
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• 2004

On the foundation of a database of genome sequences and protein analyses, the ability to clone a gene based on a peptide analysis is becoming more feasible and effective for identifying a specific gene and its protein product of interest. As such, the current study conducted a protein analysis using 2-D PAGE followed by MALDI- TOF and ESI-MS to identify a highly expressed gene product of C. parasitica. A distinctive and highly expressed protein spot with a molecular size of 47.2 kDa was randomly selected and MALDI-TOF MS analysis was conducted. A homology search indicated that the protein appeared to be a fungal enolase (enol). Meanwhile, multiple alignments of fungal enolases revealed a conserved amino acid sequence, from which degenerated primers were designed. A screening of the genomic $\lambda$ library of C. parasitica, using the PCR amplicon as a probe, was conducted to obtain the full-length gene, while RT-PCR was performed for the cDNA. The E. coli-expressed eno 1 exhibited enolase enzymatic activity, indicating that the cloned gene encoded the C. parasitica enolase. Moreover, ESI-MS of two of the separated peptides resolved from the protein spot on 2-D PAGE revealed sequences identical to the deduced sequences, suggesting that the cloned gene indeed encoded the resolved protein spot. Northern blot analysis indicated a consistent accumulation of an eno1 transcript during the cultivation.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2007년도 추계 종합학술대회 논문집
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• pp.12-16
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• 2007

단백질의 기능은 그 기능을 발휘하는 세포내의 위치와 밀접한 연관이 있다. 따라서 새로운 단백질의 서열이 밝혀지면 이 단백질의 세포내 위치를 규명하는 것은 생물학적으로 매우 중요한 일이다. 이 논문에서는 단백질의 n-그램과 kNN (k-Nearest Neighbor) 분류기를 이용한 새로운 세포내 위치예측 방법을 다룬다. 이 방법은 입력 단백질 서열과 가장 유사한 가중치를 가지는 k개의 단백질이 가지는 세포내 위치 정보들을 취합하여 입력 단백질의 세포내 위치를 추정한다. 단백질간의 유사도 가중치는 두 단백질서열의 5-그램 자질의 유사도를 비교하여 계산된다. 단백질의 세포내 위치예측 정확도를 검증하기 위해 SWISS-PROT 단백질 데이터베이스로 부터 세포내 위치가 알려진 51,885개의 서열을 추출하여 대용량 테스트 컬렉션을 구축하였으며, 다른 연구자들이 제공하는 또 하나의 소용량 테스트 컬렉션을 실험에 사용하였다. 이 논문에서 사용한 예측방법은 대용량 테스트컬렉션에 대해 약 93%의 정확도를 보여주었으며, 소용량 데스트컬렉션을 이용하여 이전 실험과 비교하였을 때도 이 방법이 다른 시스템에 비해 성능이 우월함을 알 수 있었다.

• 정보처리학회논문지D
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• 제11D권7호
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• pp.1517-1526
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• 2004

공공 데이터베이스의 이용 가능한 단백질 데이터의 양적 증가와 함께 포스트지놈 시대가 도래되면서, 단백질의 3차 구조에 대한 연구가 활발하게 진행되고 있다. 단백질의 구조를 효과적으로 파악하기 위해서 단백질의 3차 구조를 시각화할 필요가 있다. 최근 많은 시각화 도구들이 이미 그 구조가 알려진 단백질을 시각화하기 위해 Java 기술을 이용하여 개발되었다. 본 논문에서는 새로운 단백질 시각화 도구인 JProtein 시스템은 Java3D 기법을 이용하여 개발되었다. Java3D 3D 표현을 위한 프로그래밍 인터페이스를 제공하는 APIdl다. JProtein 시스템은 시각화된 아미노산 내의 원자들간의 각도 및 거리 정보를 제공하며, 단백질 분자구조에 대해 여러 가지 3차원 표현 모델을 지원한다. 특히, JProtein 시스템은 비동기식 스테레오 뷰와 함께 동기식 스테레오 뷰를 지원한다.

• Plant Biotechnology Reports
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• 제2권1호
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• pp.75-85
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• 2008

Prune dwarf virus (PDV) is an Ilarvirus systemically infecting almond trees and other Prunus species and spreading through pollen, among other means. We have studied strategies based on coat protein (cp) gene to block PDV replication in host plant cells. A Portuguese isolate of PDV was obtained from infected almond leaves and used to produce the cDNA of the cp gene. Various constructs were prepared based on this sequence, aiming for the transgenic expression of the original or modified PDV coat protein (cpPDVSense and cpPDVMutated) or for the expression of cpPDV RNA (cpPDVAntisense and cpPDVwithout start codon). All constructs were tested in a PDV host model, Nicotiana benthamiana, and extensive molecular characterization and controlled infections were performed on transformants and their progenies. Transgenic plants expressing the coat protein RNA were able to block the proliferation of a PDV isolate sharing only 91% homology with the isolate used for cpPDV cloning, as evaluated by DAS-ELISA on newly developed leaves. With cp expression, the blockage of PDV proliferation in newly developed leaves was only achieved with the construct cpPDV Mutated, where the coat protein has a substitution in the 14th aa residue, with arginine replaced by alanine. This result points to a possible role of the mutated amino acid in the virus ability to replicate and proliferate. This work reveals the possibility of achieving protection against PDV through either coat protein RNA or mutated cp sequence.

• 한국식품영양과학회지
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• 제33권10호
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• pp.1676-1684
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• 2004

어육, 닭 가슴살 및 돼지 후지 육을 산성 및 알칼리 용액으로 추출하여 등전점 부근에서 회수하고 중성 부근의 pH로 재조절하여 회수한 단백질의 가열 젤 물성과 이들의 혼합에 따른 가열 물성 값의 변화, 최적 물성과 최소비용을 제공하는 혼합 비율을 결정하였다. 갈고등어의 근원섬유단백질은 산 및 알칼리 처리에 의해 가열 젤을 형성하지 못하였으나, 산과 알칼리 처리후 근형질 단백질을 포함한 회수 단백질은 가열 젤을 형성하였다. pH 10.5에서 처리 후 회수한 단백질의 가열 젤의 파괴강도는 갈고등어가 가장 낮았고, 변형 값은 냉동 꼬마민어>닭 가슴살>돼지 후지 육>갈고등어의 순으로 높았으며, 백색도는 냉동 꼬마민어 회수 단백질이 가장 높았다. 갈고등어 회수 단백질의 첨가는 파괴강도, 변형 값, 백색도를 감소시키고 가격을 상승시키는 반면, 닭 가슴살 회수 단백질의 첨가는 파괴 강도와 백 색도를 다소 증가시키고 가격을 현저히 감소시켰다. 냉동 꼬마민어 회수 단백질인 경우, 파괴강도 110 g 이상, 변형 값 4.5 mm 이상 및 회수 단백질의 원료 단가 2000원 이하/kg을 만족하는 최적 혼합 비율은 냉동 꼬마민어 36∼50%, 닭 가슴살 34∼40%, 돼지 후지 육 14∼25%이었다 가열 젤의 구조는 냉동 꼬마민어 회수 단백질이 가장 치밀하였다. 냉동 꼬마민어 회수 단백질을 축으로 닭 가슴살, 돼지 후지 육 회수 단백질의 적절한 혼합 비율의 조절은 물성 값이 다양화한 연제품에 활용 가능할 것으로 판단된다.

• Clinical and Experimental Reproductive Medicine
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• 제26권2호
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• pp.225-230
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• 1999

In mouse, the heat shock protein 70-2 (hsp70-2) is found to have special function in spermatogenesis. Based on the observation, the hypothesis that human hspA2 (human gene; 98.2% amino acid homology with hsp70-2) might have important function in spermatogenesis in human testes was proposed. To test the hypothesis, we examined the expression of hspA2 in human tissues. Expression vector pDMC4 for expression of the human hspA2 protein using pTricHisB (invitrogen, USA) was constructed and the expressed hspA2 protein was cross-reacted with antiserum 2A raised against mouse hsp70-2 protein. Based on the cross-reactivity, we determined the expression level of hspA2 protein in human tissues by western blot analysis using the antiserum 2A. We demonstrated that antiserum 2A antibodies detected human hspA2 protein with specificity which was produced in the E.coli expression system. On Western blot analyses, significant hspA2 expression was observed in testes with normal spermatogenesis, whereas a low level of hspA2 was expressed in testis with Sertoli-cell only syndrome. Also, a small amount of hspA2 was detected in breast, stomach, prostate, colon, liver, ovary, and epididymis. These results demonstrate that the hspA2 protein is highly expressed in male specific germ cells, which in turn suggests that hspA2 protein might playa specific role during meiosis in human testes as suggested in the murine model. However, further studies should be attempted to determine the function of hspA2 protein in human spermatogenesis.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.47-47
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• 2005

There are many different surface technologies currently applied for preparation of protein chips. However, it requires innovative surface chemistry for capture proteins to be immobilized on chip surface keeping their conformation and activity intact and their orientation right, while they bind tightly and densely in a given array spot. Proteogen has developed 'ProteoChip BP' coated with novel proprietary linker molecules $(ProLinker^{TM})$ for efficient and robust immobilizations of capture proteins by improving surface properties of molecular captures. It was demonstrated that $ProLinker^{TM}$ gave the best surface performance in preparation of protein microarray chip base plates among others currently available on the market. In particular, the $ProLinker^{TM}-based$ surface chemistry has demonstrated to provide excellent performance in preparation of 'Antibody Chip' for analysis of biomarkers as well as proteome expression profiles. The linker molecule has also shown to be well applicable for development of biosensors and micro-beads as well as protein microarray and nano-array. ProteoChip BP can be used either for preparation of high-density array by using a microarrayer or for preparation of 'Well-on-a-Chip' with low density array, which is better applicable for quantitative analysis of biomarkers or protein-protein interactions. The biomarker assay can be performed either by direct or sandwich methods of fluorescence immunoassay. Application of ProteoChip BP has been well demonstrated by the extensive studies of 1) tumor-marker assays, 2) new drug screening by using 'Integrin Chip' and 3) protein expression profile analysis. Some of experimental results will be presented.