• Nutrition Research and Practice
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• v.8 no.2
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• pp.192-197
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• 2014

BACKGROUND/OBJECTIVES: The body composition changes in aging increased the risk of metabolic disorder. Recent dietary studies have increasingly focused on the correlations between dietary patterns and chronic diseases to overcome the limitations of traditional single-nutrient studies because nutrients in food have complex relations that interact. SUBJECTS/METHODS: This study was conducted to classify a dietary pattern among Korean elderly using cluster analysis and to explore the relationships between dietary patterns and body composition changes in Korean elderly aged 65 years or older. The study subjects (n=1,435) were individuals who participated in the Korean National Health Examination and Nutrition Survey (KNHANES) in 2011. RESULTS: There were three dietary patterns derived by cluster analysis in this study: 'Traditional Korean' (37.49% of total population), 'Meat and Alcohol' (19.65%) and 'Westernized Korean' (42.86%). The (1) 'Traditional Korean' pattern was characterized by high consumptions of white rice and low protein, low fat, and low milk products, while (2) 'Westernized Korean' pattern ate a Korean-style diet base with various foods such as noodles, bread, eggs and milk, (3) 'Meat and Alcohol' pattern had high consumptions of meat and alcohol. In body composition changes, compared with the 'Traditional Korean' pattern, the 'Meat & alcohol' pattern was associated with a 50% increased risk of having elevated BMI ($kg/m^2$), 'Westernized Korean' pattern was associated with a 74% increased abnormality of ASM/Wt (kg) by logistics analysis. Most of the Korean adult population continues to follow ether a traditional Korean having beneficial effects for successful aging. However, the 'Traditional Korean' pattern showed low protein intake (0.7 g/kg), calcium intake, and vitamin D intake as well as low of appendicular skeletal muscle mass (ASM (kg)) among 3 groups. CONCLUSIONS: Considering the low ASM, consumption of protein, calcium and vitamin D should be increased for Korean elderly health body composition.

• PNF and Movement
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• v.19 no.3
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• pp.311-319
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• 2021

Purpose: The study examined the effects of the proprioceptive neuromuscular facilitation pattern exercise and protein intake on balance, gait ability, and lower extremity muscular strength for sarcopenia in the elderly. Methods: A total of 30 elderly people (65~74years) with sarcopenia were recruited and randomized to Group I (n=10), Group II (n=10), and Group III (n=10). Their balance ability was measured using the functional reach test. Group I performed the proprioceptive neuromuscular facilitation pattern exercise and protein intake. Group II performed the proprioceptive neuromuscular facilitation pattern exercise. Group III performed protein intake. Their gait ability was measured using the Timed Up and Go test. Lower extremity muscular strength was measured using the Five Times Sit to Stand test. Results: As a result of comparison within groups, Group I and Group II showed a significant difference in balance, gait, and lower extremity muscular strength after the experiment (p<0.05), and Group I showed a more effectively significant difference than either Group II or Group III in balance, gait, and lower extremity muscular strength before and after the experiment (p<0.05). Conclusion: This study showed that the proprioceptive neuromuscular facilitation patterns exercise and protein intake was effective in balance, gait ability, and lower extremity muscular strength for sarcopenia in the elderly.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1460-1468
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• 2003

An experiment was conducted to quantify the flow of soluble non-ammonia nitrogen (SNAN) in the liquid phase of ruminal (RD) and omasal digesta (OD), and to investigate diurnal pattern in SNAN flow in OD. Five ruminally cannulated Finnish-Ayrshire dairy cows in a $5{\times}5$ Latin square design consumed a basal diet of grass silage and barley grain, and that supplemented with four protein feeds (kg/d DM basis) as follows: skimmed milk powder (2.1), wet distiller' solubles (3.0), untreated rapeseed meal (2.1) and treated rapeseed meal (2.1). Ruminal digesta was sampled using a vacuum pump, whereas OD was collected using an omasal sampling system at 1.0 h interval during a 12 h feeding cycle. Both RD and OD were acidified, centrifuged to remove microbes and precipitated with trichloroacetic acid followed by centrifugation. The SNAN fractions (free amino acid (AA), peptide and soluble protein) in RD and OD were assessed using ninhydrin assay. Free AA, peptide and soluble protein averaged 60.0, 89.4 and 2.1 g/d, respectively, for RD, and 81.8, 121.5 and 2.5 g/d, respectively, for OD. Although free AA flow was relatively high, mean peptide flow was quantitatively the most important fraction of SNAN, indicating that degradation of peptide to AA rather than hydrolysis of soluble protein to peptide or deamination may be the most limiting step in rumen proteolysis. Diurnal pattern in flow of peptide including free AA in OD during a 12 h feeding cycle peaked 1 h post-feeding, decreased by 3 h post-feeding and was relatively constant thereafter. Protein supplementation showed higher flow of peptide including free AA immediately after feeding compared with no supplemented diet. There were no differences among protein supplements in diurnal pattern in flow of peptide including free AA in OD.

• Journal of Microbiology and Biotechnology
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• v.22 no.8
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• pp.1107-1112
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• 2012

Outbreaks of foodborne diseases associated with Vibrio species such as V. parahaemolyticus, V. vulnificus, and V. cholerae frequently occur in countries having a dietary habit of raw seafood consumption. For rapid identification of different Vibrio species involved in foodborne diseases, whole-cell protein pattern analysis for 13 type strains of 12 Vibrio species was performed using SDS-PAGE analysis. Pathogenic Vibrio species such as V. parahaemolyticus, V. vulnificus, V. cholerae, V. alginolyticus, V. fluvialis, and V. mimicus were included in the 12 Vibrio species used in this study. Each of the 12 Vibrio species showed clearly specific band patterns of its own. Two different strains of V. parahaemolyticus showed two different SDS-PAGE whole-cell protein patterns, giving the possibility of categorizing isolated strains in the same V. parahaemolyticus species into two subgroups. The 36 Vibrio isolates collected from sushi restaurants in Busan were all identified as V. parahaemolyticus by comparing their protein patterns with those of Vibrio type strains. The identified isolates were categorized into two different subgroups of V. parahaemolyticus. The whole-cell protein pattern analysis by SDS-PAGE can be used as a specific, rapid, and simple identification method for Vibrio spp. involved in foodborne diseases at the subspecies level.

• Journal of Nutrition and Health
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• v.12 no.4
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• pp.11-19
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• 1979

A numerical analysis of essential amino acid pattern of protein in daily Korean foods was conducted through computer programming based on the food intake data from 'Reports of national nutritional survey (1969, $1973{\sim}1979)'$ and food supply data from 'Food balance sheet $(1970{\sim}1977)'$. It was noted that the amounts of daily intake and supply of animal protein were gradually increased since 1973. In 1977, animal protein intake was 17.5g in total protein intake of 68g per person per day. Intake and supply of total essential amino acids were $20{\sim}28g$ per person per day in the both of urban and rural areas, and greater consumption of leucine, phenylalanine+tyrosine, isoleusine, lysine and valine was observed. Amino acids scores of consumed protein based on FAO provisional amino acid scoring pattern(1973) were around $75{\sim}90$ showing the scores of 77.9 in 1969 and 89.5 in 1977, and also lysine(1969, $1973{\sim}1976$) and threonine(1977) were considered as first limiting amino acid. On the other hand the scores of supply Protein $(1970{\sim}1977)$ rated in $82{\sim}88$, lysine$(1970{\sim}1971)$ or threonine $(1972{\sim}1977)$ was found as first limiting amino acid. Generally a protein quality of Korean daily food was improved since 1973 and a difference between urban and rural areas in terms of the nutritive quality of protein was considerably reduced in 1977.

• Korean journal of food and cookery science
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• v.10 no.2
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• pp.126-130
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• 1994

This study was to investigate the amount of protein and essential amino acid in 19 fast-foods. fast-food samples were freeze dried, then assayed for protein and eight essential amino acids by Kjeldahl and amino acid autoanalyzer method. A.S.(amino acid score) based on FAO/WHO(1973) provi-sional Pattern & C.S.(chemical score) based on whole e99 Pattern(1972) were calculated from the amount of essential amino acid of fast-foods. The 1st limiting amino acid of the tested fast-foods was found to be SAA based on both whole e99 Pattern& FAO/WHO Provisional Pattern.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.6
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• pp.564-570
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• 1994

This experiment was conducted to determine seed storage protein pattern and structural character of differed peanut cultivars during germination. Soluble protein content in both Namdae and Daekwang cultivars remarkably decreased in cotyledon site at 2 or 3 days after incubation(DAI) and in embryonic axis site at 1 or 2 DAI, showing 28∼29% in cotyledon site and 10% in embryonic axis site at 5 DAI. Protein subunits such as 66, 43, 40 and 35.5kD bands in the cotyledon site of Namdae and Daekwang cultivars disappeared, but 21.5-23kD band disappeared slightly, but low polypeptide band such as 14-16kD increased gradually, and the same trend has been obserbed in embryonic axis site during 2 DAI. The amount of new protein formed during germination period was highest in cotyledon site at 3 DAI, and in embryonic axis site at 2 DAI. 16kD bend detected in cotyledon site of Daekwang cultivar during germination.

• Journal of Veterinary Clinics
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• v.28 no.1
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• pp.75-80
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• 2011

Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in pigs fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n = 30/group) of pigs were administered with 0 (control) and 16 ml/L (treatment) TCM-KWG through drinking water. After 4 weeks, we examined the protein expression pattern of longissimus dorsi muscle by Two-dimensional electrophoresis analysis. TCM-KWG treatment significantly increased two spot's density, and markedly reduced one spot's density in the muscles. We identified 3 proteins (heat shock protein 90-alpha, myosin binding protein and cofilin 2) by the ESI-MS/MS (Q-TOF2, Micromass). These results demonstrate that TCM-KWG treatment may play a protection role against physiological stress in pigs, like as increased heat shock protein 90-alpha.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.1
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• pp.8-14
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• 1993

To investigate selection efficiency for protein content and hardness in a wheat breeding program, two crosses were made between soft wheat (SW) ‘Chokwang’ and hard wheat (HW) ‘Lancota’, and between HW ‘Suwon 210’ and SW ‘Atlas 66’. F2 progeny lines from the crosses were separated into 4 groups as HW + high protein (HP), HW + low protein (LP), SW + HP and SW + LP by measuring protein content and sedimentation value in F4 generation. The subsequent populations were tested for protein content, sedimentation value, mixogram pattern and bread quality. HP group selected from F4 again had a higher protein content in F5 generation than LP group, but there was no significant difference between two groups in F6 generation. In sedimentation value, the significant difference was recognizable only between the groups of HW + HP and. SW + LP. Plant based investigation in F6 revealed that the distribution pattern for protein content and sedimentation value shifted from the low to high by the four groups with the order of SW + LP, HW + LP, SW + HP, HW + HP. Such a trend was most noticeable in the progenies of the cross between ‘Suwon 210’ and ‘Atlas 66’. Mixogram pattern that shows flour characteristics differed greatly among the groups, indicating a high selection efficiency for the traits. Bread volume in F6 of HW + HP was found to be higher than that of SW + LP, only from the cross between ‘Suwon 210’ and ‘Atlas 66’.

• Applied Biological Chemistry
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• v.20 no.2
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• pp.182-187
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• 1977

This investigation was to determine the changes in the trypsin inhibitor activity(TIA) and electrophoresis patterns of the soybean cotyledon and axis during germination. The TIA of the cotyledon decreased slightly and that of the axis decreased rapidly to 50% activivity after 7 day germination. At the 2nd, 3rd and 4th day's germination the TIA of the defatted dry axis was higher than that of cotyledon. However, the TIA of the fresh cotyledon was lower than that of the axis, due to its higher moisture content. Results from the electrophoretic studies showed that band 1 (polymer, 15S etc.), 2(11S), and 3(7S) whichare the major reserve proteins of soybean were decreased consid erably in cotyledon and axis and the fragments with Rm values between 0.5 and 1.0 were increased and band 5 showed up during germination. The band 4 of the cotyledon and band 6 of axis were not changed during germination. Generally speaking, the TIA and thereserve protein decreased as germination proceed.