Browse > Article

Proteome Analysis of Pigs Fed with Tissue Culture Medium Waste after Harvest of Korean Wild Ginseng  

Seol, Jae-Won (College of Veterinary Medicine, Chonbuk National University)
Chae, Joon-Seok (College of Veterinary Medicine, Seoul National University)
Kang, Hyung-Sub (College of Veterinary Medicine, Chonbuk National University)
Kang, Chun-Seong (E&T Co. Ltd.)
Ihn, Dong-Chul (College of Veterinary Medicine, Chonbuk National University)
Park, Sang-Youel (College of Veterinary Medicine, Chonbuk National University)
Publication Information
Journal of Veterinary Clinics / v.28, no.1, 2011 , pp. 75-80 More about this Journal
Abstract
Proteomics is a useful approach to know protein expression, post-translational modification and protein function. We investigated the protein expression pattern and identity in pigs fed with the tissue culture medium waste after harvest of Korean wild ginseng (TCM-KWG) (Panax ginseng). Two groups (n = 30/group) of pigs were administered with 0 (control) and 16 ml/L (treatment) TCM-KWG through drinking water. After 4 weeks, we examined the protein expression pattern of longissimus dorsi muscle by Two-dimensional electrophoresis analysis. TCM-KWG treatment significantly increased two spot's density, and markedly reduced one spot's density in the muscles. We identified 3 proteins (heat shock protein 90-alpha, myosin binding protein and cofilin 2) by the ESI-MS/MS (Q-TOF2, Micromass). These results demonstrate that TCM-KWG treatment may play a protection role against physiological stress in pigs, like as increased heat shock protein 90-alpha.
Keywords
Gingseng; TCM-KWG; Proteomics; Heat shock protein; Cofilin;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Grover AK, Samson SE. Peroxide resistance of ER Ca2+ pump in endothelium: implications to coronary artery function. Am J Physiol 1997; 273: C1250-1258.
2 Hedman K, Pastan I, Willingham MC. The organelles of the trans domain of the cell. Ultrastructural localization of sialoglycoconjugates using Limax flavus agglutinin. J Histochem Cytochem 1986; 34: 1069-1077.   DOI   ScienceOn
3 Gillis CN. Panax ginseng pharmacology: a nitric oxide link? Biochem Pharmacol 1997; 54: 1-8.   DOI   ScienceOn
4 Santos WR, de Lima VM, de Souza EP, Bernardo RR, Palatnik M, Palatnik de Sousa CB. Saponins, IL12 and BCG adjuvant in the FML-vaccine formulation against murine visceral leishmaniasis. Vaccine 2002; 21: 30-43.   DOI   ScienceOn
5 Gosse B, Gnabre J, Bates RB, Dicus CW, Nakkiew P, Huang RC. Antiviral saponins from Tieghemella heckelii. J Nat Prod 2002; 65: 1942-1944.   DOI   ScienceOn
6 Yan JX, Harry RA, Wait R, Welson SY, Emery PW, Preedy VR, Dunn MJ. Separation and identification of rat skeletal muscle proteins using two-dimensional gel electrophoresis and mass spectrometry. Proteomics 2001; 1: 424-434.   DOI   ScienceOn
7 Yun TK. Experimental and epidemiological evidence on nonorgan specific cancer preventive effect of Korean ginseng and identification of active compounds. Mutat Res 2003; 523-524: 63-74.
8 Wilkins MR, Sanchez JC, Gooley AA, Appel RD, Humphery- Smith I, Hochstrasser DF, Williams KL. Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it. Biotechnol Genet Eng Rev 1996; 13: 19-50.   DOI   ScienceOn
9 Yan JX, Tonella L, Sanchez JC, Wilkins MR, Packer NH, Gooley AA, Hochstrasser DF, Williams KL. The Dictyostelium discoideum proteome--the SWISS-2DPAGE database of the multicellular aggregate (slug). Electrophoresis 1997; 18: 491-497.   DOI   ScienceOn
10 Wasinger VC, Cordwell SJ, Cerpa-Poljak A, Yan JX, Gooley AA, Wilkins MR, Duncan MW, Harris R, Williams KL, Humphery-Smith I. Progress with gene-product mapping of the Mollicutes: Mycoplasma genitalium. Electrophoresis 1995; 16: 1090-1094.   DOI   ScienceOn
11 Scaglione F, Ferrara F, Dugnani S, Falchi M, Santoro G, Fraschini F. Immunomodulatory effects of two extracts of Panax ginseng C.A. Meyer. Drugs Exp Clin Res 1990; 16: 537-542.
12 Smirnov IP, Zhu X, Taylor T, Huang Y, Ross P, Papayanopoulos IA, Martin SA, Pappin DJ. Suppression of alpha-cyano-4- hydroxycinnamic acid matrix clusters and reduction of chemical noise in MALDI-TOF mass spectrometry. Anal Chem 2004; 76: 2958-2965.   DOI   ScienceOn
13 Hu S, Concha C, Lin F, Persson Waller K. Adjuvant effect of ginseng extracts on the immune responses to immunisation against Staphylococcus aureus in dairy cattle. Vet Immunol Immunopathol 2003; 91: 29-37.   DOI   ScienceOn
14 Rabilloud T. Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains. Proteomics 2002; 2: 3-10.   DOI
15 Rivera E, Hu S, Concha C. Ginseng and aluminium hydroxide act synergistically as vaccine adjuvants. Vaccine 2003; 21: 1149-1157.   DOI   ScienceOn
16 Kim CS, Park JB, Kim KJ, Chang SJ, Ryoo SW, Jeon BH. Effect of Korea red ginseng on cerebral blood flow and superoxide production. Acta Pharmacol Sin 2002; 23: 1152-1156.
17 Kim HE, Oh JH, Lee SK, Oh YJ. Ginsenoside RH-2 induces apoptotic cell death in rat C6 glioma via a reactive oxygenand caspase-dependent but Bcl-X(L)-independent pathway. Life Sci 1999; 65: PL33-40.   DOI   ScienceOn
18 Bae GS, Nam KP, Kim HS, Lee SG, Choi HS, Min WK, Joo W, Maeng, WJ, Chang MB. Effects of the artificial culture medium of wild ginsengs on rumen fermentation characteristics in vitro. J Anim Sci & Technol(Kor) 2003; 5: 987-996.
19 Blackstock WP, Weir MP. Proteomics: quantitative and physical mapping of cellular proteins. Trends Biotechnol 1999; 17: 121-127.   DOI   ScienceOn
20 Neuhoff V, Arold N, Taube D, Ehrhardt W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 1988; 9: 255-262.   DOI   ScienceOn
21 De Combarieu E, Fuzzati N, Lovati M, Mercalli E. Furostanol saponins from Tribulus terrestris. Fitoterapia. 2003; 74: 583-591.   DOI   ScienceOn
22 Bucci LR. Selected herbals and human exercise performance. Am J Clin Nutr 2000; 72: 624S-636S.