• Title/Summary/Keyword: Protein levels

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배합사료의 단백질 및 지질 함량이 종어(Leiocassis longirostris Gunther) 치어의 성장과 체성분에 미치는 효과 (Effect of Dietary Protein and Lipid Levels on the Growth and Body Composition of Juvenile Long Snout Bullhead Leiocassis longirostris Gunther)

  • 임상구;한형균;방인철;최진;이상민
    • 한국수산과학회지
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    • 제46권4호
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    • pp.377-383
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    • 2013
  • We ran a feeding trial to determine optimal dietary protein and lipid levels for growth of juvenile long snout bullhead Leiocassis longirostris Gunther. Eight experimental diets (P20L7, P20L14, P30L7, P30L14, P40L7, P40L14, P50L7 and P50L14) were formulated to contain 20%, 30%, 40% or 50% protein combined with either 7% or 14% lipid. Three replicate groups of fish (mean mass: 3.9 g/fish) were fed one of the experimental diets ad libitum for 8 weeks. Survival of fish fed the P20L14 diet was lower than that of fish fed the P40L14, P50L7 and P50L14 diets. Growth of fish fed diets containing 7% lipid increased with increasing protein level (up to 50% protein); growth of fish fed diets containing 14% lipid increased with increasing protein level (up to 30% protein). The feed efficiency of fish fed a diet with 50% protein and 7% lipid was higher than that of other groups. Whole body moisture and lipid contents were affected by dietary lipid level but not by dietary protein level. The crude lipid contents of fish fed 14% lipid diets were higher than those fed 7% lipid diets across all protein levels (other than the 50% level). Thus, under our experimental conditions, an increase in dietary protein level improved growth and feed efficiency of fish; a diet containing 50% protein with 7% lipid was optimal for growth and effective feed utilization in juvenile long snout bullhead.

한국 성인의 단백질 섭취량 추이 및 적절성 평가: 2010 ~ 2019년 국민건강영양조사 자료를 활용하여 (Trends in Dietary Protein Intake and Its Adequacy among Korean Adults: Data from the 2010 ~ 2019 Korea National Health and Nutrition Examination Survey (KNHANES))

  • 함현지;하경호
    • 대한지역사회영양학회지
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    • 제27권1호
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    • pp.47-60
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    • 2022
  • Objectives: This study aimed to evaluate dietary protein intake and its adequacy among Korean adults during recent 10 years. Methods: Based on the 2010 ~ 2019 Korea National Health and Nutrition Examination Survey (KNHANES) data, a total of 51,296 adults aged 19 years old or more who participated in a one-day 24-hr dietary recall were included. Dietary protein intake was estimated as percentages of total energy (% of energy) and grams per body weight (g/kg/day) and compared with the 2020 Dietary Reference Intakes for Koreans to evaluate the adequacy of protein intake. In addition, proportions of people whose protein intakes were less than the estimated average requirement (EAR) and above the upper limit of the acceptable macronutrient distribution range (AMDR) (> 20% of energy) were calculated according to sociodemographic characteristics. Results: Protein intake was increased from 14.7% of energy in 2010 to 15.6% of energy in 2019 among Korean adults. However, there was no increase in protein intake relative to the recommended nutrient intake (% RNI) during the recent 10 years. Protein intake relative to the RNI was decreased from 130.2% in 2010 to 121.1% in 2019 (P for trend < 0.0001) among total participants, and a significant decreasing trend was observed in all age groups except for over 65 years old. However, protein intake relative to the RNI was lowest in the elderly (98.6%). Proportions of low protein intake (< EAR) and high protein intake (> AMDR) increased in the past 10 years (P for trend < 0.0001 for all), and these were associated with socioeconomic statuses, such as education and household income levels. Conclusions: These findings suggest that protein adequacy in Korean adults has not been improved over the past decade compared with recommended levels. Nutritional education and intervention programs should consider different intake levels according to sociodemographic characteristics.

단백질과 지방의 수준을 달리한 식이로 사육한 흰쥐의 성장과 면역반응에 관한 연구 (The Effect of Dietary Protein and Fat Levels on the Growth and Immune Response in Rats)

  • 이현옥
    • Journal of Nutrition and Health
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    • 제21권1호
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    • pp.36-46
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    • 1988
  • 단백질의 수준과 종류, 지방의 수준에 따라서 나타나는 실험동물의 성장, 장기와 지방조직을 관찰하였으며 mitogen에 의한 lympocyte의 생산능력 및 PFC에 의한 면역능력을 관찰한 결과는 다음과 같았다. 1) 실험1의 결과, 체중증가량과 장기 무게는 지방수준에 관계없이 6%의 casein을 섭취한 군이 15%나 30% casein군에 비하여 낮았다. 그러나 15%군과 30%군 사이에는 유의적인 차이가 없었다. 2) 전체 실험을 통하여 본 연구에서는 단백질의 종류나 수준에 따른 면역능력의 차이는 나타나지 않았다. 그러나 단백질의 수준이나 질에 관계없이 고지방식이(30%)를 섭취하였을 때가 저지방식이를 섭취하였을 때에 비하여 면역능력이 낮은 경향을 보였다.

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산란계 사료의 에너지 및 단백질 수준이 하절기에 있어서 갈색산란계의 생산성에 미치는 영향 (Effects of Dietary Energy and Protein Levels on the Performance of Brown Layer in Summer)

  • 이승우;이상진;김영일;오세정
    • 한국가금학회지
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    • 제14권2호
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    • pp.125-135
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    • 1987
  • 본 시험은 산란계사료의 에너지 및 단백질 수준이 하절기에 있어서 갈색 산란계의 생산성에 미치는 영향을 구명하기 위하여 대사에너지 3수준(2,500, 2700 및 2900Kcal/kg)과 단백질 3수준(13, 15 및 17%)을 조합한 9개처리에 24주령된 Warren 산란계 720수를 공시하여 1983년 6월 3일부터 동년 8월 25일까지 12주간에 걸쳐 시험을 실시한 바 그 결과는 다음 요약과 같다. 1. 산란율은 에너지 수준이 증가할수록 점차 저하되었고 단백질 수준이 증가할수록 점차 증가하였다. 2. 란중은 에너지 수준에 따라서 큰 차이가 없었으나 단백질 수준이 증가할수록 증가하였다. 3. 사료섭취량은 에너지 수준이 증가할수록 점차 감소하였으나, 단백질 수준에 따라서는 차이가 없었다. 4. 사료요구율은 단백질 수준이 증가할수록 점차 개선되었다. 5. 폐사율은 사료중의 에너지 및 단백질 수준에 영향을 받지 않았다. 6. 1일수당 에너지 섭취량은 사료중의 에너지 수준이 증가할수록 점차 증가하였다. 7. 산란 kg당 대사에너지 요구량은 에너지 수준이 증가할수록 점차 증가하였으며 단백질 수준이 증가할수록 감소하였다. 8. 산란kg당 단백질 요구량은 에너지 및 단백질수준이 증가할수록 점차 증가하였다. 9. 산란kg당 사료비는 에너지 수준이 증가할수록 점차 증가하였고 단백질 수준이 증가할수록 점차 감소하였다.

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Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition

  • Zhou, Jia;Yue, Shuangming;Xue, Benchu;Wang, Zhisheng;Wang, Lizhi;Peng, Quanhui;Xue, Bai
    • Journal of Animal Science and Technology
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    • 제63권5호
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    • pp.1126-1141
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    • 2021
  • Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42℃ for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 ㎍/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 ㎍/mL (MET70, 70 ㎍/mL of Met), 20 ㎍/mL (MET80, 80 ㎍/mL of Met), and 30 ㎍/mL (MET90,90 ㎍/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 ㎍/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 ㎍/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 ㎍/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells.

EFFECT OF DIETARY PROTEIN AND ENERGY LEVELS ON THE PERFORMANCES OF STARCROSS LAYERS

  • Salah Uddin, M.;Tareque, A.M.M.;Howlider, M.A.R.;Jasimuddin Khan, M.;Ahmed, S.
    • Asian-Australasian Journal of Animal Sciences
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    • 제5권4호
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    • pp.723-731
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    • 1992
  • Two similar experiments were designed to evaluate the interaction of dietary CP and ME levels on the production performance parameters at the age between 155 and 300 days in Starcross layers. In both experiments, the feed intake and mortality decreased but the egg weight, body weight gain and feed conversion efficiency increased as the dietary CP and ME levels increased. The CP intake was highest CP and lowest ME levels. With the increasing CP and decreasing ME levels, the ME intake decreased significantly (Experiments 1 & 2). The $CP{\times}ME$ interactions were significant only on mean egg weight and egg production in Experiment 2. In both the experiments, the CP levels were positively correlated with CP intake, egg weight, body weight gain and egg production and negative correlation with feed intake, mortality percentage and ME intake. The ME levels showed negative correlation with feed intake, protein intake, mortality percentages and positive correlation with all other parameters in both experiments. The highest values were noted for all the parameters (except mortality percentages) in Experiment 1 than that recorded in Experiment 2.

Evaluation of physicochemical and textural properties of myofibrillar protein gels and low-fat model sausage containing various levels of curdlan

  • Lee, Chang Hoon;Chin, Koo Bok
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권1호
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    • pp.144-151
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    • 2019
  • Objective: Curdlan has been widely used as a gelling agent in various food systems. This study was performed to evaluate the rheological properties of pork myofibrillar protein (MP) with different levels of curdlan (0.5% to 1.5%) and its application to low-fat model sausages (LFS). Methods: MP mixtures were prepared with 0.5%, 1.0%, and 1.5% of curdlan. Cooking loss (%), gel strength (gf), shear stress (Pa), and scanning electron microscopy were measured. Physicochemical and textural properties of LFS containing different levels of curdlan were measured. Results: The shear stress of MP mixtures increased with increasing levels of curdlan. MP gels with increased levels of curdlan decreased cooking loss and increased gel strength (p<0.05). The MPs with 1.0% and 1.5% of curdlan were observed more compact three-dimensional structure than those with 0.5% curdlan. Increased curdlan level in LFS affected redness ($a^{\star}$) and yellowness ($b^{\star}$) values. Although expressible moisture of LFS did not differ among curdlan levels, LFSs with various levels of curdlan decreased cooking loss as compared to control sausages. Hardness values (2,251 to 2,311 gf) of LFS with 0.5% and 1.0% curdlan was increased and differ from those (1,901 gf) of control sausages. Conclusion: The addition of 1.0% curdlan improved the functional and textural properties of LFS.

Dietary Exposure to Transgenic Rice Expressing the Spider Silk Protein Fibroin Reduces Blood Glucose Levels in Diabetic Mice: The Potential Role of Insulin Receptor Substrate-1 Phosphorylation in Adipocytes

  • Park, Ji-Eun;Jeong, Yeon Jae;Park, Joon Beom;Kim, Hye Young;Yoo, Young Hyun;Lee, Kwang Sik;Yang, Won Tae;Kim, Doh Hoon;Kim, Jong-Min
    • 한국발생생물학회지:발생과생식
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    • 제23권3호
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    • pp.223-229
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    • 2019
  • Type 2 diabetes mellitus (T2DM) is characterized by insulin resistance (IR). T2DM is correlated with obesity and most T2DM medications have been developed for enhancing insulin sensitivity. Silk protein fibroin (SPF) from spiders has been suggested as an attractive biomaterial for medical purposes. We generated transgenic rice (TR) expressing SPF and fed it to diabetic $BKS.Cg-m+/+Lepr^{db}$ mice to monitor the changes in blood glucose levels and adipose tissue proteins associated with energy metabolism and insulin signaling. In the present study, the adipocyte size in abdominal fat in TR-SPF-fed mice was remarkably smaller than that of the control. Whereas the adenosine monophosphate-activated protein kinase (AMPK)-activated protein kinase and insulin receptor substrate 1 (IRS1) protein levels were increased in abdominal adipose tissues after TR-SPF feeding, levels of six-transmembrane protein of prostate 2 (STAMP2) proteins decreased. Phosphorylation of AMPK at threonine 172 and IRS1 at serine 307 and tyrosine 632 were both increased in adipose tissues from TR-SPF-fed mice. Increased expression and phosphorylation of IRS1 at both serine 307 and tyrosine 632 in adipose tissues indicated that adipocytes obtained from abdominal fat in TR-SPF-fed mice were more susceptible to insulin signaling than that of the control. STAMP2 protein levels decreased in adipose tissues from TR-SPF-fed mice, indicating that STAMP2 proteins were reducing adipocytes that were undergoing lipolysis. Taken together, this study showed that TR-SPF was effective in reducing blood glucose levels in diabetic mice and that concurrent lipolysis in abdominal adipocytes was associated with alterations of AMPK, IRS1, and STAMP2. Increased IRS1 expression and its phosphorylation by TR-SFP were considered to be particularly important in the induction of lipolysis in adipocytes, as well as in reducing blood glucose levels in this animal model.

Voluntary exercise training improves body weight of leptin-deficient ob/ob mice by altering hepatic stearoyl-CoA desaturase 1 and deleted in breast cancer 1 protein levels

  • Lim, Wonchung;Hwang, Moon-Hyon;Kang, Chounghun;Kim, So Yeon;Cho, Hyeseong
    • 운동영양학회지
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    • 제25권4호
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    • pp.54-58
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    • 2021
  • [Purpose] Deleted in breast cancer 1 (DBC1) ablation causes obesity, and stearoyl-CoA desaturase 1 (SCD1) induces the biosynthesis of monounsaturated fatty acids. This study examined whether voluntary wheel running (VWR) alters SCD-1 and DBC1 protein levels in the liver of leptin-deficient ob/ob mice. [Methods] Twenty-five Ob/Ob mice were divided into two groups (ob/ob-Sed and ob/ob-Ex). The expression of DBC1 and SCD1 in the mouse liver was determined using western blotting. [Results] After 10 weeks, VWR significantly reduced body weight without affecting the fatty acid synthase and CD36 protein levels. The average daily running distance was 4.0±1.0 km/day. This improvement was associated with changes in the hepatic SCD1 and DBC1 levels. Hepatic SCD-1 protein levels increased significantly, and DBC1 protein levels decreased in ob/ob-Sed animals. On the other hand, VWR inhibited the obesity-induced increase in SCD1 expression and impaired the obesity-induced decrease in DBC1 expression in the liver of leptin-deficient ob/ob mice. [Conclusion] This is the first study showing that VWR has strong effects on hepatic SCD1 and DBC1 in ob/ob mice, and provides key insights into the effects of exercise on obesity.

EFFECTS OF DIETARY THYROXINE ON GROWTH PERFORMANCE AND CARCASS QUALITY OF BROILERS FED DIFFERENT LEVELS OF DIETARY PROTEIN

  • Kim, J.W.;Lee, C.H.;Han, I.K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제6권4호
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    • pp.527-539
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    • 1993
  • To investigate the effects of crude protein and thyroxine on growth performance, nutrient utilizability, carcass composition, the content of total fat and cholesterol in leg muscle, breast muscle and liver, and caloric efficiency in broiler chicks. The experiment involved 3 levels of dietary crude protein (1-3 weeks: 20, 23, 26%; 4-6 weeks: 17, 20, 23%) and 3 levels of thyroxine (0.0, 1.5, 3.0 mg/kg). In the starting period (1-3 weeks), body weight gain of chicks fed diets containing 26% crude protein and 1.5 mg/kg thyroxine was higher than any other groups, and among thyroxine levels, 3.0 mg/kg thyroxine groups were lower. The best feed efficiency was obtained at 26% crude protein with no thyroxine supplemented or 1.5 mg/kg thyroxine supplemented groups. In the finishing period (4-6 weeks) the highest body weight gain was obtained at 23% crude protein with no thyroxine supplemented group. Feed intake of 17% crude protein with 1.5 mg/kg thyroxine supplemented group was higher than those of the other groups. It was found that the utilizability of crude protein in the starting period, showed the best utilizability at 20% crude protein with 1.5 mg/kg thyroxine group. Increasing crude protein level from 17 to 23%, utilizability of crude fat was decreased. The carcass composition was significantly (p<0.05) influenced by crude protein and thyroxine. Increasing thyroxine level from 0.0 to 3.0 mg/kg, crude protein content was increased whereas, crude fat content was decreased. Chicks fed diet containing 1.5 mg/kg thyroxine showed the lowest total fat content in liver tissue. In breast muscle, it was significantly (p<0.05) affected by crude protein and thyroxine. Present data revealed that the cholesterol content was increased for the chicks fed 3.0 mg/kg thyroxine. It the caloric efficiency, chicks fed a diet containing 20% crude protein with no thyroxine supplementation showed the highest caloric efficiency and the lowest efficiency was from 23% crude protein group with 1.5 mg/kg thyroxine. From this study it may be concluded that crude fat content of carcass could be successfully reduced by dietary supplementation of thyroxine, whereas crude protein content was increased.