• Asian-Australasian Journal of Animal Sciences
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• v.18 no.7
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• pp.933-941
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• 2005

To develop a functional cDNA chip, specific genes expressed in the tissues of swine Kagoshima Berkshire were screened. A total of 4,434 ESTs were obtained by constructing a cDNA library from total RNA isolated from the muscle and fat tissues, affirming their functions by investigating similarity of nucleotide sequences with the database at the NCBI. Among them, 1,230 ESTs were confirmed as novel genes, which, to date, have not been identified. Attaching the genes to a cDNA microarray slide revealed expression patterns of genes in muscle and fat according to the growth stages of swine. As specific genes expressed in the muscle tissues of swine with body weight of 30 kg, 60 genes including actin, myosin, tropomysin, transfer RNA-trp synthetase, Kel-like protein 23, KIAA0182 and COI, Foocen-m, etc were obtained. In addition, 18 novel genes were obtained. As specific genes expressed in fat tissues of swine with body weight of 30 kg, 47 genes including annexin II, Collagen, Fibronectin, Pleckstrin homology domain, serine protease, etc were obtained. 21 novel genes were also obtained. The genes specifically expressed in the muscle and fat tissues of swine affect contraction and relaxation of the muscle and the fat. However, studies on the expression mechanisms of the genes are insufficient. To reveal species of structural genes in swine muscle and fat tissue, interrelation studies in expression and function of genes by using the cDNA chip should be conducted.

• Korean Journal of Veterinary Service
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• v.34 no.1
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• pp.1-4
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• 2011

A surface plasmon resonance imaging (SPRI) assay was developed for measuring porcine circovirus type 2 (PCV2) antibody using a recombinant capsid protein as an antigen. The diagnostic potential of SPRI for detecting antibodies to the PCV2 capsid protein was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 70 pig serum samples taken from 6 pig farms. There was a strong positive correlation between the SPRI and ELISA (n = 70, r = 0.911, P<0.01). Therefore, this recombinant capsid protein can be used as an antigen for serological studies, and the SPRI, a label-free and high-throughput method, is expected to be a valuable tool in the serodiagnosis of PCV2 infection.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.473-473
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• 2005

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.467-467
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• 2005

• Proceedings of the Korean Society of Toxicology Conference
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• 2005.05a
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• pp.196-196
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• 2005

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2004.06a
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• pp.196-200
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• 2004

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.118.1-118.1
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• 2007

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• Proceedings of the KIEE Conference
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• 2003.07c
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• pp.1944-1946
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• 2003

질병의 발현과 직접적인 관련이 있는 단백질의 검출, 정량화하는 분석 장비를 소형화 할 수 있는 방법을 소개하고 그 가능성을 실험적으로 확인하였다. 단백질 검출을 위한 형광측정방법에서 가장 큰 문제인 여기광과의 혼재로 인한 신호 대 잡음 비를 해결하기 위해 마이크로 프리즘을 이용한 여기 방식을 고안하고 설계, 시뮬레이션 하였으며, 선행 실험을 통해 프리즘의 이용한 형광검출방법이 신호 대 잡음 비의 향상과 분석 시스템의 소형화에 효과적임을 확인하였다.

• Proceedings of the PSK Conference
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• 2002.04a
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• pp.156.1-156.1
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• 2002

• Journal of Life Science
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• v.17 no.2 s.82
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• pp.235-240
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• 2007

AvrRpt2 protein triggers hypersensitive response (HR) and strong disease resistance when it is translocated from a bacterial pathogen Pseudomonas sp. to host plant cells containing a cognate RPS2 resistance protein through Type III Secretion System (TTSS). However, AvrRpt2 protein can function as the effector that suppresses a basal defense and enhances the disease symptom when functional RPS2 resistance protein is absent in the infected plant cells. Using Affymetrix Arabidopsis DNA chip, we found that many genes were specifically regulated by AvrRpt2 protein in the rps2 Arabidopsis mutant. Here, we showed that expression of AtERF11 that is known as a member of B1a subcluster of AP2/ERF transcription factor family was down regulated specifically by AvrRpt2. To determine its function in plant resistance, we also generated the Arabidopsis thaliana transgenic plants constitutively overexpressing AtERF11 under CaMV 355 promoter, which conferred an enhanced resistance against a bacterial pathogen, Pseudomonas syringae pv. tomato DC3000. Thus, these results collectively suggest that AtERF11 plays a role as a positive regulator for disease resistance against biotrophic bacterial pathogen in plant.