• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.1
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• pp.1-7
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• 1993

In this study, the food intake, feces and urine of the 7 high school boys were collected and the intake and excretion of protein and calcium were measured. The boys were 16 to 18 years old and the measurement was conducted for four weeks during which they maintained their normal living pattern and body weight. Each boy's daily intake and excretion of protein and calcium were measured and digestibility and balance were also studied. 1)Daily mean intake, fecal loss and apparent digestibility of protein of each boy were 73, 9$\pm$1.9g, 10.9$\pm$0.4g and 84.9$\pm$0.5%, respectively. The urinary loss of nitrogen was 8.76$\pm$0.22g and showed the positive balance of 1.33$\pm$0.28g. 2) Daily mean intake, fecal loss and apparent digestibility of calcium of each boy were 515.3$\pm$19.3mg, 221.6$\pm$14.1mg and 57.1 $\pm$2.7% respectively. The urinary loss of calcium was 102.6$\pm$8.4mg and showed the positive balance of 192.4$\pm$19.5mg.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.10
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• pp.1433-1438
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• 2002

The study was carried out to evaluate leucaena seeds as a protein replacement of mustard seed cake (MSC) in the concentrate mixture of growing lambs. Fifteen owing male lambs (Local${\times}$Corridale) with an average body weight of 16.3 kg were allocated into three dietary treatments (T1, T2, and T3) with five animals in each group. Animals were offered dry mixed grass, berseem hay and concentrate mixture to meet their nutrient requirements. In concentrate mixture of T1, (Control) MSC was used as protein source, while in T2 and T3 groups, 25 and 50% of MSC was replaced by leucaena leucocephala seeds. On completion of three months (90 days) of feeding, a digestion cum-metabolism trial was conducted to determine DMI, nutrient utilization, and nitrogen balance. Changes in body weight were recorded at 15 day internals and eating patterns were recorded for 3 consecutive days at the end of the feeding trial. MSC had higher CP contents than leucaena seeds (27.0%). Mimosine contents in leucaena seeds were 1.1 compared to 0.2 and 0.4% in concentrate mixture of T2 and T3 group, respectively. Dry matter intake varied non-significantly ($79.3{\pm}1.2$ to $83.4{\pm}1.3g/kg$ $w^{0.75}$) across the dietary treatments. Digestibility of DM and cell wall polysaccharides (NDF, ADF. Cellulose and hemicellulose) were comparable, however CP digestibility was relatively lower in leucaena luecocephala seeds based groups (T2 $45.5{\pm}1.7$ and T3 $46.7{\pm}3.5$) compared to MSC supplemented group (T1 $47.7{\pm}0.9%$). The growth rate of lambs was non-significantly higher in T1 ($79.2{\pm}5.4$) compared to T2 ($73.8{\pm}8.8$) and T3 ($73.9{\pm}7.0$), respectively. The animals were in positive nitrogen balance and N-balance varied from 1.8 to 2.9 g/d across treatment groups. The eating rate (% of total offered) of concentrate up-to 15 min was relatively higher in T1 (82.4) than T2 (74.2) and T3 (77.8%). However no effect of leucaena seeds was recorded on total DMI of animals. The results of the study revealed that the inclusion of up to 50% leucaena seeds, as protein source in concentrate mixture of lambs had no adverse effect on DMI, nutrient utilization, eating patterns, nitrogen balance and growth performance of lambs.

• Journal of Animal Science and Technology
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• v.48 no.4
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• pp.533-540
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• 2006

This experiment was conducted to determine crude protein requirements for maintenance (CPm) of growing Hanwoo steers. Eighteen Hanwoo steers (173.7kg) were allocated randomly to diets with three levels of crude protein in the concentrate by replacing ground corn with corn gluten meal: 0% (LCP), 7.2% (MCP) and 14.2% (HCP), respectively. Hanwoo steers in each treatment were fed diets containing rice straw (20%) and concentrates (80%). CP intake was 5.5, 7.8 and 10.7g/BW0.75 for LCP, MCP and HCP, respectively. Protein balance was －0.05, 2.36 and 4.07g/BW0.75 for LCP, MCP and HCP, respectively. Intercept of the regression equation between CP intake and retained CP indicated that CPm was 5.56g/BW0.75. The estimate of CPm adopted by Korean Feeding Standard (2002) was almost 20% higher than that adopted by Japanese Feeding Standard for Beef Cattle (2000); this would need to be verified with more feeding trial data set including protein requirements for growth.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.8
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• pp.1167-1173
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• 2009

This study was conducted to evaluate the effects of providing fermented soy protein to weaned pigs on nitrogen balance and apparent fecal and apparent ileal digestibility (AID) of AA. Four weaned ((Yorkshire${\times}$Landrace)${\times}$Duroc) barrows (BW = 6.58${\pm}$0.98 kg), surgically fitted with a simple T-cannula approximately 15 cm prior to the ileo-cecal junction, were fed four diets according to 4${\times}$4 Latin square design. Diets were a basal diet supplemented with one of the following: 3% SDPP (spray dried plasma protein), 5% RBP (soy protein fermented by Lactobacillus spp.), 5% PSP (soy protein fermented by Aspergillus oryzae and Bacillus subtilis), and 2.5% RPP (2.5% RBP+2.5% PSP). No differences were observed in DM and N intakes among treatments. However, the level of urine excretion was greater in the RPP group than in the PSP group. Additionally, fecal DM excretion, fecal N concentration and fecal N excretion were increased in the RBP, PSP and RPP groups when compared with the SDPP group (p<0.05). Furthermore, total excretion was increased in the RPP group when compared with the PSP group (p<0.05). In addition, N absorption and the N absorption ratio were higher in the SDPP group than in the RPP group (p<0.05). Moreover, the DM and N digestibilities were lower in the RBP, PSP and RPP groups than in the SDPP group (p<0.05), and the ash and energy digestibilities were higher in the SDPP and RBP groups than in the PSP and RPP groups (p<0.05). However, no significant differences were observed in the DM, N, Ash, Ca, P or ileal digestibilities among treatments, although the energy digestibility was higher in the SDPP group than the RBP group (p<0.05). In addition, the apparent ileal digestibilities of essential amino acids (Arg, His, Iso, Leu, Lys, Phe, Thr, and Val) were significantly higher in the SDPP group than in the other groups (p<0.05), and the levels of Ala, Cys, Glu and Try were greater in the SDPP treatment group than the RBP, PSP and RPP groups (p<0.05). Additionally, the levels of Asp, Gly and Ser were higher in the SDPP group than the PSP and RPP groups, and the level of Pro was higher in the SDPP group than the RPP group (p<0.05). Finally, total non-essential amino acid and total amino acid digestibility were higher in the SDPP group than in the other treatments (p<0.05). Taken together, the results of this study indicate that animal protein is more bioavailable than plant protein. However, the N absorption ratio and ileal digestibility were found to be similar in the SDPP and RBP groups.

• Korean Journal of Agricultural Science
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• v.51 no.2
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• pp.193-203
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• 2024

This study examined the effect of a dietary protein level and age on growth performance, total excreta, and nitrogen balance in laying hen during the growing period for 18 weeks. A total of 96 Hy-Line Brown laying hens were divided into two treatments according to the experimental diets, with each group consisting of six replicates. The experimental diets included: 1) an adequate metabolizable energy and crude protein diet formulated to meet the age and breed specification for Hy-Line Brown containing 21, 18, 16, and 14% dietary CP during 0 to 6, 6 to 12, 12 to 15, 15 to 18 weeks, respectively (CON); 2) 2% CP deficiency from CON diet containing 19, 16, 14, 12% dietary CP during 0 to 6, 6 to 12, 12 to 15, 15 to 18 weeks, respectively (LP). Growth performance, total excreta, and nitrogen balance were investigated for 18 weeks every 3 weeks, and two-way ANOVA was used for statistical analysis of data. The LP group had lower total excreta and N intake than CON group in the whole period of the experiment (p < 0.001). Moreover, birds in the LP group showed lower N excretion than the CON group (p < 0.05). However, the CON group exhibited higher BW, ADG than the LP group (p < 0.001). Application of low-protein diet to laying hens during the growing period reduced N excretion, which contribute to the sustainable development of the laying hen industry by minimizing environmental pollution.

• Proceedings of the EASDL Conference
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• 2005.04a
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• pp.21-34
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• 2005

Increase in daily protein consumption per capita from 1975(85.1 g) to 2001(88.4 g) was 3.3 g. This trend was relatively slower than the case of Japan where daily protein consumption was 84.7 g in 1975 and 90.3 g in 2001. Animal-related protein in 2003 was 45.7 g in which 61% was originated from meat, milk and egg whereas 39% was composed of fish and its relevance. The trend of protein consumption fairly come up with the ideal ratio of 5:5 between animal-originated protein and plant-originated protein, following the base case of Japan. The effect of animal protein on human health can vary depending on one's viewpoint and its controversy is still a subject of debate. For reason, two faces of positive and negative effects on human health coexists. However, there is no doubt that positive effect is far more than negative one. It is not important whether or not animal protein is more beneficial for human health. However, it is more important how human balance between two proteins.

• Mycobiology
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• v.38 no.4
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• pp.302-309
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• 2010

Formic acid is a representative carboxylic acid that inhibits bacterial cell growth, and thus it is generally considered to constitute an obstacle to the reuse of renewable biomass. In this study, Saccharomyces cerevisiae was used to elucidate changes in protein levels in response to formic acid. Fifty-seven differentially expressed proteins in response to formic acid toxicity in S. cerevisiae were identified by 1D-PAGE and nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) analyses. Among the 28 proteins increased in expression, four were involved in the MAP kinase signal transduction pathway and one in the oxidative stress-induced pathway. A dramatic increase was observed in the number of ion transporters related to maintenance of acid-base balance. Regarding the 29 proteins decreased in expression, they were found to participate in transcription during cell division. Heat shock protein 70, glutathione reductase, and cytochrome c oxidase were measured by LC-MS/MS analysis. Taken together, the inhibitory action of formic acid on S. cerevisiae cells might disrupt the acidbase balance across the cell membrane and generate oxidative stress, leading to repressed cell division and death. S. cerevisiae also induced expression of ion transporters, which may be required to maintain the acid-base balance when yeast cells are exposed to high concentrations of formic acid in growth medium.

• The Korean Journal of Food And Nutrition
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• v.11 no.6
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• pp.647-686
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• 1998

The purpose of this study was to investigate selected nutrient intakes, dietary atherogenesity, energy balance and activity level of college students. One hundred and thirty onoe students (61 males and 70 females) made their three-day dietary records and one-day activity records along with one-day dietary records. BMI of the surveyed students was 22.33 for males and 20.28 for females. Average calorie intakes of male and female students was respectively 2166.20kcal and 1793.60kcal. Females had slightly higher fiber intakes than males in terms of fiber g/1000kcal. Carbohydrate, protein and fat ratios in energy composition of males and females were respectively 56.62 : 15.33 : 24.70 and 57.64 : 16.49 : 25.79. Average cholesterol intakes of male and female students were 295.66mg and 259mg respectively. The male showed higher saturated fat intake. and had higher atherogenic indices of diets such as cholesterol index(CI) and cholesterol-saturated fat index(CSI) than the female. Males spent an average of 1039.00 kcal/day compared to females of 687.93 kcal/day for all physical activities. Most students participated in light (100% of males and females) and moderate (33% of males and 3% of females) activities level. Few students spent time at severe and very severe activity level. Seventeen of the 131 students were in positive energy balance whereas 83% were in negative balance. Both genders with positive energy balance tended towards smaller weight and BMI, and larger food intakes than those with negative energy balance. Students with positive energy balance had higher saturated fat intakes and dietary atherogenic scores of CI and CSI than the counterparts with negative energy balance. Males with positive energy balance had decreased physical activity and daily energy expenditure whereas increased food and saturated fat intakes. Females with positive energy balance had singificantly increased food, saturated fat and sugar intakes.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.3
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• pp.274-276
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• 1997

The effects of cecectomy on nitrogen utilization and nitrogen excretion were examined in single comb white leghorn (SCWL) cockerels fed a 5% protein diet supplied with urea. The cecectomy tended to increase nitrogen balance and nitrogen utilization and significantly decreased uric acid excretion (p < 0.01). Urea and ammonia excretion tended to be about 60% increased and decreased by cecectomy in SCWL cockerels, respectively, but blood ammonia, urea and uric acid concentrations were not affected. The results are in good agreement with those obtained previously in cecum-ligated chickens. It is concluded that the improvement of nitrogen utilization and decreases in urinary uric acid excretion in cecectomized chickens do not result from the modification of cecal fermentation.

• Journal of Nutrition and Health
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• v.24 no.2
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• pp.124-131
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• 1991

This study was to examine the effects of dietary protein levels and the sources of protein(animal and plant) on calcium and phosphorus metabo lism in 10 healthy Korean female adults. The 26­d day study consisted of a 6-day adaptation period and lO-day moderate protein(109 N, 550mg Ca) a and IO-day high protein( 14g N, 570mg Ca) pe­r riod. During the experimental period, the subjects w were divided into two groups, either consuming a animal protein diet(75 % animal protein) or plant protein diet(75 % plant protein). Calcium(300 mg) was supplemented to two subjects of each d dict group for the last 4 days. Feces, urine and diet were analyzed nitrogen. calcium and phos­p phorus. The apparent absorption of calcium was significantly increased as the protein intake was inc­r reased from 60g to 90g in animal protein diet. A Average calcium absorption rate was 30% and 46 % from high and moderate levels of animal p protein diet Urinary calcium excretion was not affected by t the amount of the protein intake in both animal and plant protein diet. There was significantly higher urinary calcium excretion(I34mg) in high I level of animal protein diet than that( 83mg) in h high level of plant protein diet. Calcium balance was improved as the protein intake increased and c calcium was supplemented. Phosphorus absorp­t tion was more efficient in the high animal diet(77.81 %) than in the high plant diet(55-65%). The overall results indicate that an increase of protein and calcium supplement in moderate pro­ttein intake can improve calcium balance due to the increase of calcium absorption.