This experiment was carried out to determine the forage production and soil characteristics in mixtures of barley and annual legumes at riverbed. The dry matter (DM) and total digestible nutrients (TDN) in barley and hairy vetch mixture were higher compared to other mixtures. Barley, single cropping was the highest in DM content. Comparing different mixtures, barley and crimson clover mixture was higher than other mixtures in DM contest. The crude protein (CP) content of barely and hairy vetch mixture was highest, but ether extract (EE) and TDN contents were lower than other mixtures. Barely and hairy vetch mixture significantly removed organic matter and phosphorus from riverbed through forage production (P<0.05). The total nitrogen (TN) of barley and hairy vetch mixture was highest because of high nitrogen fixation in riverbed soil. Single barley was highest in organic matter (OM) of soil. Comparing different mixtures, barley and crimson clover mixture was higher than other mixtures in OM contest. The available P2O5 of soil in barley and crimson clover mixture and barley and rape mixture were higher than others, and that of barley and hairy vetch was lower than other mixtures. The barely and hairy vetch mixture was highest in cation exchange capacity (CEC) content. Therefore barley and hairy vetch mixture is more suitable in forage mixtures because of high forage production and protein, and reduction of soil phosphorus in riverbed.
Ahn, Byung Hun;Moon, Dong Ju;Yoo, Kye Sang;Ho, Chia Chi
Korean Chemical Engineering Research
/
v.43
no.2
/
pp.236-242
/
2005
Membrane fouling by protein mixtures during microfiltration has been investigated for binary mixtures of bovine serum albumin (BSA), casein, lysozyme, pepsin, and ovalbumin. Filtration experiments were carried out using $0.2{\mu}m$ polycarbonate track-etched (PCTE) membrane in a stirred cell under constant transmembrane pressure (14 kPa) and concentration of hydrogen ion (pH=11) to study the effect of mixture composition on filtrate flux decline. Flux decline data were analyzed using a pore blockage-cake formation model developed recently. It was found that the model is in a good agreement with the experimental data. Fouling parameters such as the rate of pore blockage(${\alpha}$), the initial resistance of the protein deposit ($R_{po}$) and the increasing rate of the protein layer resistance(${\beta}$) were used to evaluate the rate of filtrate flow by membrane fouling in the binary mixture system. Generally, the trend of ${\alpha}$ is comparable with that of filtrate flux decline. It was also found that fast flux decreasing was observed over the binary mixture containing casein. The result is due to high value of the initial resistance of the protein deposit ($R_{po}$) over casein.
Journal of The Korean Society of Grassland and Forage Science
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v.14
no.4
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pp.264-270
/
1994
This experiment was undertaken to determine the effect of pasture ages on the total production and seasonal yield trends, botanical composition and forage quality of pasture mixtures. This experiment was conducted at the Livestock Experiment Station, RDA, Suweon. The results obtained were as follows : 1. Total dry matterDM) yield of pasture mixtures was the highest in the first year, and thereafter no significant reduction in the 2nd through the 4th year, but decreased by 30% in the 5th year. 2. The 34.4% of total DM yield was produced at the first cut, and decreased from then on. Total DM yield of pasture mixtures varied by year and climatic facton. In 1992, Total DM and TDN yields of pasture mixtures were 13.4 and 8.5 IvlT/ha, respectively. 3. The content of crude protein(CP), in virro DM digestibility(IVDMD) and TDN of pasture mixtures were 12-14, 70-80, and 62-65%, respectively. The highest forage quality was obtained at the 4th cutting. 4. The botanical compositions of legumes and weeds were increased as the year of establishment advanced, and highly increased at the 3rd cut after summer growth. 5. Based on the results of this study it appears that economic forage production could be expended to the 4th year with proper management, and the special renovation techniques may be needed for maintenance of pasture yield from the 5th year.
The objectives of this study were to determine the interaction between porcine myofibrillar proteins and various gelatins (bovine hide, porcine skin, fish skin, and duck skin gelatins) and their impacts on gel properties of porcine myofibrillar proteins. Porcine myofibrillar protein was isolated from pork loin muscle (M. longissimus dorsi thoracis et lumborum). Control was prepared with only myofibrillar protein (60 mg/mL), and gelatin treatments were formulated with myofibrillar protein and each gelatin (9:1) at the same protein concentration. The myofibrillar protein-gelatin mixtures were heated from $10^{\circ}C$ to $75^{\circ}C$ ($2^{\circ}C/min$). Little to no impacts of gelatin addition on pH value and color characteristics of heat-induced myofibrillar protein gels were observed (p>0.05). The addition of gelatin slightly decreased cooking yield of heat-induced myofibrillar protein gels, but the gels showed lower centrifugal weight loss compared to control (p<0.05). The addition of gelatin significantly decreased hardness, cohesiveness, gumminess, and chewiness of heat-induced myofibrillar gels. Further, sodium dodecyl poly-acrylamide gel electrophoresis (SDS-PAGE) showed no interaction between myofibrillar proteins and gelatin under non-thermal conditions. Only a slight change in the endothermic peak (probably myosin) of myofibrillar protein-gelatin mixtures was found. The results of this study show that the addition of gelatin attenuated the water-holding capacity and textural properties of heat-induced myofibrillar protein gel. Thus, it could be suggested that well-known positive impacts of gelatin on quality characteristics of processed meat products may be largely affected by the functional properties of gelatin per se, rather than its interaction with myofibrillar proteins.
The aim of this study was to evaluate the chemical composition, in vitro DM degradability, ME and OMD of alfalfa-maize silage mixtures in comparison to pure maize and alfalfa silages, and to test the existence of associative effects of ensiling alfalfa forage with whole-crop maize using the in vitro gas production technique. Ensiling alfalfa with whole-crop maize had a significant (p<0.001) effect on chemical composition, pH, in vitro DM degradability, OMD and estimated ME values of mixtures. DM content of the resultant silages significantly increased with increasing proportion of whole-crop maize in the mixtures, whereas the pH value, crude protein (CP), acid detergent fibre (ADF) and ash contents of mixtures decreased due to the dilution effect of whole-crop maize which was low in CP, ADF and ash. The pH values of all alfalfa-maize silage mixtures were at the desired level for quality silage. Gas production of alfalfa-maize silage mixtures at all incubation times except 12 h increased with increasing proportion of whole-crop maize. When alfalfa was mixed with whole-crop maize in the ratio 40:60, ME and OMD values were significantly (p<0.001) higher than other silages. Maximum gas production ($A_{gas}$) ranged from 65.7 to 78.1 with alfalfa silage showing the lowest maximum gas production. The results obtained in this study clearly showed that maximum gas production increased with increased percentage of whole-crop maize in the silage mixtures (r = 0.940, p<0.001). It was concluded that ensiling alfalfa with whole-crop maize improved the pH, OMD and ME values. However, trials with animals are required to see how these differences in silage mixtures affect animal performance.
Journal of The Korean Society of Grassland and Forage Science
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v.34
no.1
/
pp.9-14
/
2014
This experiment was carried out to order to determine the forage production and soil characteristics in mixtures of Italian ryegrass (IRG) and annual legumes at the riverbed. The IRG and hairy vetch mixture had the highest in fresh, dry matter (DM) as well as total digestible nutrients (TDN) yields among the forage mixtures. Further IRG and hairy vetch mixture had the highest in DM content. The crude protein (CP) content of IRG and hairy vetch mixture was the highest, Whereas non-fiber carbohydrate (NFC) and TDN contents were lower compared to other mixtures. The total nitrogen (TN) of IRG and hairy vetch mixture was the highest due to high nitrogen fixation in the riverbed soil. The IRG and rape mixture was the highest in organic matter (OM) of soil. Moreover, the available $P_2O_5$ of soil in IRG and crimson clover mixtures was higher compared to other mixtures. Further, the IRG and hairy vetch mixture was the highest in cation exchange capacity (CEC) content. Therefore IRG and hairy vetch mixture is more suitable in forage mixtures due to high forage production and protein as well as high soil CEC content at the riverbed.
In this study, food protein hydrolysates were prepared from six types of food protein: purified meat protein, whole egg protein, casein, isolated soy protein, concentrated rice protein, and gluten. Food proteins were hydrolyzed with pepsin and ethanol (80%)-soluble fractions of pepsin hydrolysates were employed for analysis. The products were colorless and odorless powders with low fat content and good solubility. The MW (molecular weight) of the protein hydrolysates was confirmed to be $200{\sim}1,800$ via gel filtration. Free amino acid contents accounted for less than 5% of the samples. The results of our amino acid analysis revealed that all food protein hydrolysates preserved their original amino acid compositions and nutritional values of their source proteins with highly pure oligopeptide mixtures. These results show that the food protein hydrolysates prepared in these investigations should prove excellent dietary nitrogen sources for a variety of applications.
Korea is one of the typical rice eating countries where availability of animal protein is limited even for the growing generation. Nutritive food products for infants and children are not available commercially in large scale at the present time, although a limited amount of expensive milk products are produced. The present study deals with a pioneering attempt on a new food mixture to meet this demand. Several food mixtures, possibly produced in the country, consisting of rice, soybean, FPC, vitamin, minerals and other food additives are developed in this work Sixty female and male rats aged $30{\sim}40$ days were divided into five groups, twelve rats each. The rats had been maintained with the six different diets, as follow. Formulation of proposed infant food mixturesComposition F-R-1 F-S-2 F-F-3 F-P-4 S Rice 100% 40% 37% 46% 70% Sugar - 12 13 10 70 Casein - - - - 20 Bean - 40 37 24 - Yeast - 3 2 3 - Mineral Vitamain - 2 4 2 4.3 F.P.C - 3 4 7 - Fat - - 4 8 4 Cod liver Oil - - - - 3 The findings of this study presented in FER, PER, final organ weights, body weights growth, percentage of nitrogen retention in the body. F-P-4 group tended to remain the heaviest and F-R-1 the lightest in the body weight among three groups throughout the experimental period. In terms of FER (Feed Efficiency Ratio) and PER (Prortein E. R.) value, F-P-4 group kept the highest record throughout. F-P-4 group showed the highest value of nitrogen retention in the body. In comparision between F-P-4 groups and casein 20% group (standard) in all respects of this experiment, F-P-4 group which is the most superior among experimental group, revealed statisfically no significant inferior than that of standard group. This fact could be interpreted that most limiting a. a., methionine in the soy protein produced little inferiority of the experimental groups in the study.
Processing and storage of the silage from agricultural by-products were studied in two experiments. The first experiment was conducted to investigate the chemical composition and degradability of various silages with varying ensiling times. The experiment was a 5$\times$3 factorial design, completely randomized, with factor A as the different formulated mixtures by varying level of urea addition (0, 0.5, 1.0, 1.5 and 2.0%) and factor B as the time of ensiling. Chemical composition changed little with time and varied only slightly with levels of urea in the mixtures. Dry matter (DM) degradability increased with increasing cassava levels while crude protein degradability and pH level increased with increasing urea addition. By using 'Flieg point', which relates to organic acid yields, there were no significant difference among ensiled mixtures and times of ensiling. Therefore it can be concluded that the 5th silage formulation is the most appropriate since its DM and crude protein (CP) degradability were highest. The second experiment was carried out to determine the quality of the 5th silage mixtures (from the previous trial) after being stored for up to 6 months. The experiment was a complete randomized design with samples taken at monthly intervals up to 6 months and subjected to laboratory and degradability analyses. The results showed no significant (p>0.05) difference in chemical composition except for increased neutral detergent fiber and acid detergent fiber percentage in association with increasing storage time. There were no significant (p>0.05) differences in 'Flieg point' among times of storage. In conclusion, this experiment showed that the silage from agricultural by-products can be stored for more than 6 months.
The purpose of this study was to improve the production of Mozzarella cheese analogues manufactured using mixtures of soy milk and concentrated raw milk by performing ultrafiltration (UF) and to assess the quality of these cheeses during a 30-day storage period at $4^{\circ}C$, relative to that of Mozzarella cheese manufactured with the traditional method. The solid consistency of Mozzarella cheese analogue prepared from milk mixtures was lower than that of cheese manufactured from raw milk or soy milk and increased during storage, which is considered to be the result of decreasing water levels, as well as with increasing soy milk concentrations. In the Mozzarella cheese analogue generated using the milk mixtures, the fat content decreased with increase in the soy milk concentration, while it decreased during the storage period. Lactose levels were lowest in cheese composed of soy milk or raw milk and processed by UF, and decreased during storage in cheese produced using milk mixtures. In milk mixtures containing soy milk, the protein concentration increased with increasing amounts of raw milk and did not change during the storage period. The water-soluble nitrogen compound level was similar between cheeses and increased only slightly during storage. The amount of non-protein nitrogen compounds was higher in the cheese analogue than in the control cheese and tended to increase during storage. Analysis of the physicochemical traits of the Mozzarella cheese analogue yielded the following results: During storage, titratable acidity levels increased while pH tended to decrease. After analysis using electropherograms, it was classified as ${\alpha}$-, ${\beta}$-, or ${\kappa}$-casein. The results of rheometry tests showed that in the Mozzarella cheese analogue prepared from milk mixtures, with raw milk concentrated by UF, increases in concentration rate lead to lowered hardness, elasticity, cohesiveness, and brittleness. When cheese was produced from milk mixtures and concentrated by UF, meltability increased as the concentration rate increased, although to an extent that was less than that observed for the control cheese, and tended to increase during storage. Sensory evaluation showed that the analogue cheese was much better than the control cheese in terms of formation, appearance, and flavor.
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