• Journal of Animal Science and Technology
• /
• 제62권4호
• /
• pp.533-542
• /
• 2020

The objective of this study was to observe the interactions between salt-soluble proteins extracted from beef and elephant foot yam (Amorphophallus campanulatus) flour in heat-induced gel matrix development. The effect of salt concentration; 0.5%, 1.0%, 1.5%, and 2.0% in weight/weight basis (w/w), during protein extraction on pH, salt-soluble protein concentration and myofibril fractions of beef extract was determined firstly, and no significant effect was found. The beef salt-soluble proteins extracted using salt solution at different concentrations were then added with elephant foot yam flour at 5%, 10%, and 15% w/w, gelatinized at 90℃ for 20 min, and cooled down at 4℃ for 12 h. The interactions between beef salt-soluble proteins and elephant foot yam flour resulted in an improved gel strength (p < 0.01) and the addition level of elephant foot yam flour affected the pH, instrumental color, moisture, crude protein, and ash content significantly. The addition of elephant foot yam flour also reduced the size of the pores in the gel matrix as shown by scanning electron microscope (SEM) photographs. These suggest that elephant foot yam flour well interacts with beef salt-soluble proteins to form gel matrix.

• 한국동물학회지
• /
• 제23권1호
• /
• pp.1-12
• /
• 1980

큐티클 形成 및 硬化過程中 血蛋白質의 變化와 起源을 규명하고자 acrylamide gel electrophoresis와 immunodiffusion 方法을 使用하였다. Acrylamide gel electrophoresis에서 적어도 19개의 protein band가 혈림프에서 發見되었으며 脂肪體에서는 13개의 band가 確認되었는데 이들은 대체적으로 일정한 pattern을 유지하였다. 또한 혈림프와 脂肪體의 一般的인 protein band의 pattern은 $3\\sim4$개의 강하게 染色된 band와 몇 개의 가는 band가 gel의 상단에 存在하는 것이 특징이었고 적어도 5개 이상의 haemolymph protein band가 　期初에 걸쳐 일정하게 나타났다. Immunodiffusion test에서는 $8\\sim9$개의 血蛋白質에 　期初에서 나타났는데 그중 2개의 血蛋白質은 　期前에 나타났으며 다른 두 血蛋白質도 　化직 후 脂肪體에서 나타남으로써 脂肪體가 이들 血蛋白質의 起源임을 암시하였다.

• Journal of Ginseng Research
• /
• 제18권2호
• /
• pp.108-112
• /
• 1994

Rats (Sprague Dawley, male, 200 g) were fed with 15% corn oil containing a large quantity of 18 2 (linoleic acid) for 3 weeks, and were followed by feeding the petroleum ether extracts from Korean red ginseng for 3 weeks. cGMP was produced more in platelets prepared from both 15% corn oil and petroleum ether extracts-fed group than in platelets only 15% corn oil-fed group, indicating that the production of cGMP is increased by feeding the petroleum ether extracts. When this platelet was stimulated by phorbol-12-myristate-13-acetate (PMA), the level of cGMP was decreased. However, the platelets in medium containing protein fraction (200 $\mu\textrm{g}$/ml) was stimulated by PMA, the production of cGMP inhibited by PMA was increased by 3 times or more. These results suggest that both the protein fraction and the petroleum ether extracts from Korean red ginseng are synergistic in the productiorl of cGMP, and they may have the antiplatelet effects.

• 한국식품영양과학회지
• /
• 제17권1호
• /
• pp.43-49
• /
• 1988

Peach seed four 의 단백질에 관한 연구를 한 결과는 다음과 같다. 일반성분중 조단백질의 함량은 20.38%였다. 염용해성 단백질의 추출율은 약 70%였으며 단백질 정제 결과 주된 분획의 수득율은 51%였다. 전기영동 결과 11개의 band가 확인 되었다. 분자량은 $14,000{\sim}110,000$사이인 것으로 나타났다. peach seed four 와 peach seed 분리물의 amino acid 분석결과 arginine, asparic acid, glutamic acid, glycine, leucine 의 함량이 높은 것으로 나타났다. Nitrogen solubility 는 pH가 5.5일때 가장 낮게 나타났다.

• Applied Biological Chemistry
• /
• 제12권
• /
• pp.13-17
• /
• 1969

한국산(韓國産) 벼 20품종(品種)(주(主)로 도입(導入) 품종(品種)) 즉 수륙도(水陸稻) 찹쌀 및 멥쌀 각(各) 5품종(品種)씩에 대하여 그 조단백질량(粗蛋白質量)과 각획분(各劃分) 단백질의 량(量)을 분석 검토하였다. 멥쌀보다 찹쌀 그리고 수도(水稻)보다 육도(陸稻)가 평균으로 단백질함량이 많았으며 oryzenin 과 albumin의 각(各) 함량비(含量比)도 같은 경향을 보였다.

• 약학회지
• /
• 제55권3호
• /
• pp.203-212
• /
• 2011

Adipocytes have been known to secrete a number of important proteins called adipokines with roles in energy metabolism, reproduction, cardiovascular function and immunity. In this study we have attempted to identify intensively secretory proteins from 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into mature adipocytes and then the cells were left in serum-free medium. The supernatant was filtrated and dialyzed. Lyophilized secretome was fractionated by two different methods, 1-D SDS PAGE and RP-FPLC. The tryptic peptides from the gel slices and the FPLC fractions were analyzed by nanoLC/ESI-MS/MS. We identified a total of 303 identical proteins from two methods, 251 proteins from 1-D gel and 184 proteins from RP-FPLC. 86 of them were listed as a secretory protein Finally, we identified many known or unknown secreted proteins existed in the low level including adiponectin, angiotensinogen, bone morphogenetic protein-1 (BMP-1), macrophage migration inhibitory factor (MIF), insulin like growth factor-II (IGF-II), interleukin-6 (IL-6), follistatin-related protein-1, minecan, and resistin. The existence of some of secreted proteins has been confirmed in RNA level. This proteomic experiment is useful for the intensive screening of secretory proteins in many kinds of other cells.

• 약학회지
• /
• 제42권5호
• /
• pp.487-493
• /
• 1998

In the previous study we described the antitumor effect of GLB, a protein-polysaccharide fraction separated from the growing tips of Ganoderma lucidum, against sarcoma 18 0 solid tumor in ICR mice. In this study, we separated an acidic protein-polysaccharide fraction, GLB-A, and a basic protein-polyaccharide fraction, GLB-B, from GLB by differential precipitation, and elucidated their antitumor and immunomodulatory activities. When ip injected at the dose of 50mg/kg/day into the ICR mice, GLB-A and GLB-B inhibited the growth of ip implantated sarcoma 180 cells by 32.4% and 21.0%, respectively. Of these, GLB-A increased the % lymphoblast in the spleen of the tumor-bearing and the normal mice by 20.9% and 123.0%, and the CD4/CD8 ratio by 73.3% and 22.4%, respectively. GLB-A also increased the expression of CD25 (IL-2 receptor alpha ch0ain) in normal mice by 82.0%. These results strongly suggest that GLB-A is a promising candidate for antitumor immunomodulatory medicine.

• BMB Reports
• /
• 제29권6호
• /
• pp.535-539
• /
• 1996

The activation of phospholipase D (PLD) catalyzes hydrolysis of phosphatidylcholine (PC) to phosphatidic acid (PA) and choline in plants as well as animals. To determine the presence of PLD in oat cells, we prepared inside-out plasma membrane and cytosolic fractions from oat tissues. PLD activities in both cytosol and plasma membrane were detected by ion chromatography method. The activity of PLD in plasma membrane was dependent upon $Ca^{2+}$ concentration and was heat stable. To investigate whether G-protein couples to PLD, the effects of $GTP{\gamma}S$ and $GDP{\beta}S$ on the PLD activity were measured. PLD activity was dramatically increased 300~400% in the presence of 50 ${\mu}M$ $GTP{\gamma}S$ but not in the presence of 50 ${\mu}M$ $GDP{\beta}S$. These results indicate that G-protein may be involved in regulation of PLD activity. To identify whether PLD is regulated by red light receptor, phytochrome, we irradiated red, far-red, or red/far-red/red light on oat protoplasts. PLD activity has increased 5-fold and 3-fold by treatment with red light and red/far-red/red light, respectively. In contrast, irradiation with far-red light had little or no effect on PLD activity. These results suggest that phytochrome regulates PLD activity through activation of G-protein in oat cells.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2000년도 NEW STRATEGY FOR DRUG DEVELOPMENT IN POST-GENOMIC ERA(대한약학회)
• /
• pp.180.3-181
• /
• 2000

• 한국식품과학회지
• /
• 제25권4호
• /
• pp.360-365
• /
• 1993

Phytate 함량이 다른 두 분리 대두단백(HSPI; high-phytate soy protein isolate, LSPI; low-phytate soy protein isolate)을 제조하여 pH 및 phytate 첨가량이 그들의 용해도와 소화율에 미치는 영향을 알아보았고 분리 대두단백을 용해도에 따라 분별한 후 그들의 특성을 전기영동을 통하여 알아보았다. LSPI와 HSPI 모두 분자량이 $13.6{\sim}81.1kDa$에 이르는 단백질 성분으로 이루어져 있으며 산성 pH에서 $18.0{\sim}35.0kDa$의 단백질은 phytate와 잘 결합하지 않는 특성을 가졌다. LSPI를 용해도에 다라 분별하였을 때 gliadin을 구성하는 단백질은 pH2에서 phytate와 쉽게 결합하지 않았다. 대두 단백질은 pepsin 소화율은 phytate 첨가량이 증가할수록 크게 저하되었다.