• 제목/요약/키워드: Protein Content

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Preparation of Accelerated Salt-fermented Anchovy Sauce Added with Shrimp Byproducts (새우가공부산물을 이용한 속성 멸치액젓의 제조)

  • Kim, Hye-Suk;Yang, Soo-Kyeong;Park, Chan-Ho;Han, Byung-Wook;Kang, Kyung-Tae;Ji, Seung-Gil;Sye, Youn-Eon;Heu, Min-Soo;Kim, Jin-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.8
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    • pp.1265-1273
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    • 2005
  • The purpose of this study was to prepare accelerated salt-fermented anchovy sauce using a shrimp processing byproducts (head, shell and tail) as a fermenting accelerator, and to investigate its physicochemical and enzymatic properties. Four types of sauces were prepared with 0, 10, 20, and 30$\%$ (w/w) addition of shrimp byproduct and fermented at 24$\pm$2$^{\circ}C$ for 360 days. During fermentation, all four type sauces decreased moisture content (67.5$\%$68.0$\%$ to 64.0$\∼$64.8$\%$) and pH (5.52$\∼$7.10 to 5.03$\∼$6.58), but showed increase in their crude protein (7.0$\∼$8.2 to 10.8$\%$) and volatile basic nitrogen contents (40$\∼$75 to 180$\∼$200 mg/100 g of sauce). The ratio of amino nitrogen to total nitrogen contents of control (0$\%$) and sauce with 10$\%$ shrimp byproducts (10$\%$ sauce) were maximized at 270 days, whereas 20$ \% $ and 30$\%$ added sauces were at 180 days. Endoprotease and exoprotease activities of anchovy sauces added with 20$\%$ and 30$\%$ of shrimp byproducts tend to be higher than those of control (0$\%$) and 10$\%$ addition. Proteolytic activities of sauces at pH 9 were about 2 times higher than those at pH 6. Amidolytic activities for LeuPNA decreased remarkably during fermentation, and control (0$\%$) almost lost their activity at 180 days, while additional sauces were relatively stable. These suggest that alkaline pretense of anchovy and shrimp byproducts as a endoprotease mainly contributed to the fermentation of salt-fermented sauces. The protein molecular weight distribution of sauces indicated 2 groups of peaks (peak 1,>70,000 da and peak 2, 3,000$\∼$29,000 da). As the fermentation proceeded, peak 1 tended to decrease in all of sauces, but peak 2 increased rapidly from 30 to 270 days. Optimum fermentation periods of control and 10$\%$ sauces were 270 days and those of 20$\%$ and 30$\%$ sauce were 180 days. The results suggest that shrimp byproduct can be used as accelerator of salt-fermented sauce.

Chemical Compositions of Fermented Codonopsis lanceolata (발효더덕의 화학성분)

  • Park, Sung-Jin;Seong, Dong-Ho;Park, Dong-Sik;Kim, Seung-Seop;Gou, Jing-Yu;Ahn, Ju-Hee;Yoon, Won-Byung;Lee, Hyeon-Yong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.3
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    • pp.396-400
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    • 2009
  • Dodok (Codonopsis lanceolata Bench. et Hook) root contains abundant pharmaceutical substances and is widely used as a food and a medicinal herb. To identify the major components, fermented Codonopsis lanceolata was analyzed for its chemical compositions prior to their pharmaceutical substances, which were used as the fundamental data. The contents of carbohydrate, crude protein, crude lipid and ash are 79.3%, 13.0%, 2.40% and 5.3%, respectively. The calories of fermented Codonopsis lanceolata was 390.5 kcal. Total dietary fiber was 47.4% of total carbohydrates. The protein was composed of 18 different amino acids. The contents of essential and non-essential amino acids were 8,118.18 mg and 10,913.42 mg. The K was the largest mineral followed by P, Ca and Mg, which means fermented Codonopsis lanceolata is alkali material. The contents of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids were 37.76%, 3.84%, and 35.64%, respectively. Therefore, the amount of the total unsaturated fatty acid was higher than that of any other plant. The content of crude saponin in fermented Codonopsis lanceolata was 60.1 mg/g. It is expected that a follow up study on fermented Codonopsis lanceolata through development and evaluation of processed foods for their functional properties would provide useful information as a source of medicinal foods.

Effect of Cooking Methods with Various Heating Apparatus on the Quality Characteristics of Pork (가열기구에 따른 조리방법이 돼지고기의 품질특성에 미치는 영향)

  • Jeon, Ki-Hong;Kwon, Ki-Hyun;Kim, Eun-Mi;Kim, Young-Boong;Choi, Yun-Sang;Sohn, Dong-In;Choi, Jin-Young
    • Culinary science and hospitality research
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    • v.21 no.1
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    • pp.1-14
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    • 2015
  • Pork belly(PB) and pork shoulder(PS) parts were tested to find out chemical and physical characteristics and sensory evaluation with various cooking methods such as pan heating, boiling, grilling, steaming, charcoal heating, pan heating and double-layer pan filled with phase change material (PCM) cooking. The moisture contents of steamed PB and PS had higher results of 60.2% and 67.2% than other treatments. The highest results of crude fat contents in PB treatments was charcoal roasting as 33.2% (p<0.05) while grilling resulted the highest at 16.0% in the PS (p<0.05). In the crude protein contents, boiling treatment resulted the lowest at 15.4% while steaming was the highest at 18.9% in PB. Also, crude protein content of grilling treatment was 25.2%, a result significantly higher than in other cooking methods in PS. Heating loss, which has a close relationship with water holding capacity, showed the highest result in the charcoal treatment at 40.18% and 39.68% each in the both of PS and PS. In the result of shear force, the lowest result was oven treatment at $2.76kg/cm^2$ in PB (p<0.05) and double-layer pan heating at $3.67kg/cm^2$ in PS (p<0.05). L value in the color test of boiling treatment showed the highest result of 65.16 and 59.72 in the PB and PS respectively (p<0.05), however it scored the lowest of 2.32 in b value in PB (p<0.05). In the 9 point-scale sensory evaluation, grilling treatment showed the highest result of 7.56 in the overall palatability of PB (p<0.05). However, PS in the pan heating which scored 7.22 was the best result while having the lowest score of 5.88 in the boiling treatment (p<0.05).

Effects of Harvest Stage on Agronomic Characteristics, Yield and Feed Value of Silage Corn in the Newly Reclaimed Hilly Land (산지 신개간 토양에서 사료용 옥수수 수확시기가 생육특성, 생산성 및 사료가치에 미치는 영향)

  • Do, Gu-Ho;Kim, Eun-Joong;Lee, Sang-Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.3
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    • pp.253-264
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    • 2012
  • This study was carried out to investigate growth characteristics, yield, chemical compositions and nutrients yield of corn hybrids for silage in the newly reclaimed hilly land. The experimental design was arranged in a randomized block design with three replications. The seeding time was at May 6. The harvest time of four treatments was milk stage (97 days), dough stage (105 days), yellow stage (112 days) and late yellow stage (119 days after seeding). Plant height, ear height, leaf numbers and ear length were highest in yellow stage (p<0.05, 0.01), but dead leaf, stem hardness and sugar degree (Brix) were higher in late yellow than other treatments. Leaf width, tip filling degree and fresh yield were not significantly different. Dry matter yield increased as the maturity stage progressed (p<0.01). Crude protein and crude fat were not significantly different. NDF and ADF decreased as the maturity stage progressed (p<0.01). Ca content was the highest at milk stage (p<0.05), Fe and P were the highest at dough stage (p<0.05, 0.01). However another minerals were not significantly different. Essential amino acid (EAA), nonessential amino acid (NEAA) and total amino acid were highest at yellow stage, but no significant differences were found among the treatments. Total free sugar contents were higher in the order of Milk > dough > yellow > late yellow stage, but no significant differences were found among the treatments. Crude protein yield was the highest at yellow stage, but crude fat yield, amino acid yield and TDN yield were highest at late yellow stage (p<0.01). Total mineral yield showed no significant difference. Based on the above results, yellow and late yellow stage compared to other maturity stage have been shown to increase dry matter yield and nutrients yield, when silage corn grow cultivate in the newly reclaimed hilly land.

Manufacture of Cutting-Gouda Cheese using Lactobacillus rhamnosus_p1 and the Physicochemical Properties of Gouda Cheese during Ripening Periods (Lactobacillus rhamnosus_p1을 이용한 절단형 고다치즈 제조방법 및 숙성 중 품질특성)

  • Park, Jong-Hyuk;Jung, Hoo-Kil;Moon, Hye-Jung;Oh, Jeon-Hui;Lee, Joo-Hee;Kim, Myung-Kon;Na, Sang-Eon;Kim, Youn-Jeong;Hwang, Young-Tae
    • Journal of Dairy Science and Biotechnology
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    • v.32 no.2
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    • pp.131-139
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    • 2014
  • The aim of this study was to manufacture Cutting-Gouda cheese and to investigate the change in physicochemical properties of Cutting-Gouda cheese made with Lactobacillus rhamnosus_p1. Lactic acid bacteria were isolated from Gouda cheese ripened for more than 1 year. They were identified as 2 strains of L. rhamnosus, Lactobacillus casei, Lactobacillus curvatus, and Staphylococcus saprophyticus by 16S rDNA sequencing and named L. rhamnosus_p1, L. casei_p2, L. curvatus_p3, L. rhamnosus_p4 and S. saprophyticus_p5. The proteolytic activities of isolated strains against casein were measured using prepared skim milk agar plates. L. rhamnosus_p1 showed the highest proteolytic activity. Cutting-Gouda cheese was made with L. rhamnosus_p1, and its physicochemical properties (moisture, protein, fat, ash and free amino acid content) were measured during ripening periods. Because of the modified atmosphere packaging ($N_2{^-}$), there was no change in moisture, protein, fat, and ash in the experimental group. The total amount of free amino acids in the control and experimental group gradually increased during ripening periods. The sensory evaluation showed that the experimental group was preferable to the control group. This result suggests that L. rhamnosus_p1 has potential to be developed as a new starter for Gouda cheese.

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Effect of Variety and Harvest Date on the Forage Production and Quality in Winter Rye (호밀의 품종 및 수확시기가 사초생산성 및 품질에 미치는 영향)

  • Lee, B.S.;Kim, J.D.;Kwon, C.H.;Chung, K.W.
    • Journal of Animal Science and Technology
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    • v.46 no.2
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    • pp.227-234
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    • 2004
  • This experiment was conducted at Cheonan Yonam Experimental Livestock Farm in 2001-2002 to detennine the effect of harvest date(20 April, 26 April and 4 May) on forage yield and quality of rye varieties of three different maturing groups(early maturing variety = ‘Koolgrazer’ midseason maturing variety = ‘Horni122’ and late maturing variety = ‘Danko’). The heading date of Kooigrazer, Hornil22 and Danko were 17, 22 and 29 April, respectively. Dry matter percentage increased from 15.6 to 21.6% as the harvest date was delayed from April 20 to May 4. Among the rye varieties tested, dry matter percentage of Koolgrazer, Hornil22 and Danko were 20.9, 18.8 and 16.3%, respectively. Dry matter yield increased from 1l.2 to 13.9 ton/ha as the harvest date was delayed, but no significant difference among rye varieties. As the harvest date was delayed, total digestible nutrients(TDN) yield also increased significantly from 7.4 to 8.4 ton/ha However no significant difference was found among rye varieties. Crude protein(CP) percentage decreased from 20.3 to 17.1% as the harvest date was delayed, and CP percentage of late maturing variety, Danko, was significantly higher than that of the other varieties. In content of fiber component(NDF, ADF, ADL, hemicellulose and cellulose) of rye, the late harvest date(4 May) showed the highest among harvest dates. From comparisoo within rye varieties tested, Kooigrazer, a early maturing variety had higher than a rnidseason and late maturing varieties, Hornill22 and Danko. The cellulase digestible of organic matter of dry matter(CDOMD) decreased as the harvest date was delayed. Among the rye varieties tested, the CDOMD of a early maturing variety, Koolgrazer was the lowest. Our study differences of winter rye in forage yield and quality resulting from variety maturity and harvest stage. A early maturing variety, Kooigrazer should be harvested between 24 and 28 April, Hamill 22(midseason maturing variety) harvested between 29 April and 3 May, Danko(late maturing variety) harvested between 4 and 8 May for maximum forage yield and optimum quality.

Effects of Dietary Probiotic Complex on Growth Performance, Blood Immunological Parameters and Fecal Malodor Gas Emission in Growing Pigs (복합 생균제 첨가가 육성돈의 생산성, 면역관련 혈액학적 지표 및 분내 유해가스 발생에 미치는 영향)

  • Jang, H.D.;Kim, H.J.;Cho, J.H.;Chen, Y.G.;Yoo, J.S.;Kim, I.H.
    • Journal of Animal Science and Technology
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    • v.49 no.4
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    • pp.501-508
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    • 2007
  • This study was conducted to evaluate effect of probiotic complex(Lactobacillus acidophilus, Bacillus subtilis and Aspergillus oryzae) on growth performance, blood immunological parameters and fecal malodor gas emissions in growing pigs. Forty-eight pigs[(Landrace × Yorkshire) × Duroc, 25.31±1.29kg average initial body weight] were used in 35d growth trial. Dietary treatments included CON(basal diet), PC1(basal diet + 0.1% probiotic complex) and PC2(basal diet + 0.2% probiotic complex). From d 0 to 20, ADFI was significantly increased in PC1 and PC2 compared to CON(Linear effect, P=0.013). From d 21 to 35, ADFI was increased in PC1 compared to CON(Quadratic effect, P=0.024). For the whole period, ADFI was increased PC2 and PC1 compared to CON(Linear effect, P=0.009, Quadratic effect, P=0.004). For the whole period, ADG was increased in PC1 compared to CON(Quadratic effect, P=0.017). G/F was not affected by treatments. Dry matter digestibility in PC2 was higher than PC1 and CON(Linear effect, P=0.001). Nitrogen digestibility was significantly higher in PC2 and PC1 than CON(Linear effect, P=0.005). In blood immunological parameters, Total protein, IgG, red blood cell(RBC) and white blood cell(WBC) were increased in PC2 compared to PC1 and CON(Linear effect, P<0.001, Quadratic effect, P<0.001). In fecal malodor gas emission, ammonia and acetic acid were significantly reduced in PC2 compared to CON(Linear effect, P<0.02). Hydrogen sulfide was significantly reduced in PC2 compared to CON(Linear effect, P=0.0002, Quadratic effect, P=0.018). However, total mercaptans was not affected by treatments. Water content of feces was not significantly different among the treatments. In conclusion, 0.2 % probiotic complex improved ADFI, apparent dry matter and nitrogen digestibility, Total protein, IgG, RBC and WBC. Also, it decreased ammonia, acetic acid and hydrogen sulfide emissions in growing pigs.

Changes in Components and Peptides during Fermentation of Cheonggookjang (청국장 발효시의 성분 변화 및 펩티드의 생성)

  • Ann, Yong-Geun
    • The Korean Journal of Food And Nutrition
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    • v.24 no.1
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    • pp.124-131
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    • 2011
  • We analyzed content and peptides in order to investigate the productivity from Cheonggookjang(fast-fermented soybean paste), fermenting it for 180 hours at $40^{\circ}C$. Results showed that pH was 7.07 at the start and became 7.41 in 24 hours, it eventually increased to 8.63 after 180 hours. Acidity was 0.2 in 12 hours, 0.5 in 12 hours, and then remained on 0.1 thereafter. Total sugar was 1.54 mg/$m\ell$ at the start, but it gradually decreased to 0.76 mg/$m\ell$ after the lapse of 48 hours, and 1.0 mg/$m\ell$ in 120 hours, and finally 0.8 mg/$m\ell$ in 180 hours. Reducing sugar was 0.14 mg/$m\ell$ at the start, and 0.88 mg/$m\ell$ after the lapse of 24 hours, 0.64 mg/$m\ell$ in 48 hours, 0.26 mg/$m\ell$ in 72 hours, and showed no definite change untill 180 hours. The amount of free amino acid was $0.19\;{\mu}M/\ell$ at the start, and $4.88\;{\mu}M/\ell$ after the lapse of 72 hours, $4.5\;{\mu}M/\ell$ in 120 hours, and then it rapidly decreased to $0.23\;{\mu}M/\ell$ after180 hours. Absorbance of soluble protein and peptide at 280 nm was 12.4 in 48 hours, 31.12 in 120 hours, and 31.12 in 180 hours. HPLC revealed that in the fermentation process, large molecular proteins are hydrolyzed into small peptides and amino acids, and after the lapse of 48 hours the pattern became almost the same. The protease activity of Cheonggookjang was 0.011 unit/$m\ell$ after the lapse of 36 hours and then it decreased. The result shows as Cheonggookjang started its deamination of amino acid in 100 hours, it is desirable to produce peptide within 100 hours of its fermentation.

Studies on the Palatability and Texture of Korean Rice Cultivars for the Cooked-rice Processing (가공밥 제조를 위한 밥의 식미 및 식감특성 평가)

  • Sim, Eun-Yeong;Park, Hye Young;Kim, Mi-Jung;Lee, Choon-Ki;Jeon, Yong Hee;Oh, Sea Kwan;Won, Yong Jae;Lee, Jeong Heui;Ahn, Eok Keun;Woo, Koan Sik
    • The Korean Journal of Food And Nutrition
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    • v.30 no.5
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    • pp.880-888
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    • 2017
  • Palatability and texture analysis of nine Korean rice cultivars were investigated to select the most appropriate rice variety for the cooked-rice processing. The rice cultivars studied consisted of Samkwang, Seonpum, Wolbaek, Andabyeo, Dasan-1, Ilpum, Haiami, Jungsanggold and Chindle. They revealed the moisture, protein, amylose and starch contents of 10~12, 5~6, 12~19, and approximately 90%, respectively. Among the evaluated rice varieties, WB exhibited the lowest amylose content (12.7%) and Jungsanggold the next (17.2%). In the rapid-visco analyzer test, Jungsanggold, Chindle, Wolbaek and Seonpum revealed a low value of final and setback viscosities than other varieties. Using a toyo meter analyzer, Chindle, Haiami, Samkwang were selected as having high toyo palatability values, while Dasan-1, Wolbaek and Andabyeo revealed low values. Toughness and adhesiveness of all nine cooked rice varieties were highest in Jungsanggold, Chindle and Ilpum. Also, palatability of cooked rice was highest in the following order: Chindle (80.03) > Samkwang (76.21) > Jungsanggold (74.08). The results of this study suggest that Chindle may be effectively used to produce processed cooked rice.

Changes in Serum Lipid Components and Blood Glucose by Krill (Euphausia superba) Meal and NaF in Rats (크릴 분말 및 NaF 첨가 식이가 흰쥐의 혈청 지질성분 및 혈당 변동에 미치는 영향)

  • Jin, Dong-Hyeok;Oh, Da-Young;Lee, Young-Geun;Kang, Dong-Soo;Kim, Han-Soo
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.4
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    • pp.938-946
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    • 2017
  • This study was conducted to examine the changes in serum lipid components and blood glucose by krill (Euphausia superba) meal and sodium fluoride (NaF) in rats fed on experimental diets and administered orally to NaF 10 mg for 5 weeks. Body weight of rats decreased as the amount of krill meal diet increased, it was observed the basal diet plus NaF group (BF group) compared to the lower basal diet group (BD group). The serum concentrations of total cholesterol, LDL (low-density lipoprotein) cholesterol, free cholesterol, triglyceride (TG), phospholipid (PL) and blood glucose in serum were higher in the BF group than the 10% krill meal group plus NaF 10 mg (KF10 group) or BD group, the 20% krill meal plus NaF 10 mg group (KF20 group), the 30% krill meal plus NaF 10 mg group (KF30 group). Conversely depending on the content of krill meal for the HDL (high-density lipoprotein) cholesterol level, it showed higher results. The concentration of total protein was no significant difference among the groups (p<0.05). The results indicate that a krill meal diet effectively inhibited increases in lipid elevation and blood glucose level in the sera of rats.