• International Journal of Industrial Entomology and Biomaterials
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• 제44권2호
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• pp.44-54
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• 2022

The food source of white-spotted flower chafer, Protaetia brevitarsis is important to economical performance. The P. brevitarsis larva were feed by mulberry and oak fermented sawdust. The effect of additional additives, Korean black raspberry (called bokbunja) marc and blueberry marc to fermented sawdust feed on the development and oviposition characteristics were investigated. The development periods of P. brevitarsis was decreased from 165.7 d to 40.0 d (mulberry sawdust) and 169.3 d to 39.0 d (oak sawdust), but the survival rate of larva was not affected by the addition of Korean black raspberry marc to fermented sawdust. However, blueberry marc addition to the fermented sawdust did not significant effect on the development period and survival rate of P. brevitarsis. The percentage of larva weight over 2.5 g was tend to increase by addition of Korean black raspberry marc and/or blueberry marc. The number of oviposition was also tend to increase by adding additional feed to sawdust but there was no significant different.

• 한국응용곤충학회지
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• 제45권2호
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• pp.139-143
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• 2006

흰점박이꽃무지(Protaetia brevitarsis) 비상활동은 6월 중순부터 9월 상순이며, 장수풍뎅이 (Allomyrina dichotoma) 비상활동은 6월 중순부터 8월 하순까지이다. 두 종의 비상활동은 모두 강수에 영향을 받아 강수량과 강수일수가 적은 해에는 비상활동이 활발하였다. 흰점박이꽃무지 3령충은 온도에 따른 분포깊이에 영향이 없었으나, 장수풍뎅이 3령 유충은 온도에 민감하게 반응하여 고온에서는 대부분의 개체들이 표면에 분포하였다. 흰점박이꽃무지 3령충은 과습한 상태가 분포깊이에 크게 영향을 주지 않았으나, 장수풍뎅이 유충은 과습한 상태에는 대부분이 표면 근처에 분포한다. 흰점박이꽃무지 유충은 죽은 개체의 속을 파먹는 것을 볼 수 있으며 돼지고기, 닭고기, 생고등어, 식빵, 사과, 배 등을 투여한 결과, 흰점박이꽃무지는 모두 잘 섭식하나 장수풍뎅이는 빵만을 섭식하였다. 두 종은 유사한 환경에서 서식하나 식이는 상당히 큰 차이를 나타내었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.108-114
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• 2022

Protaetia brevitarsis seulensis larva is well-known as an edible insect. The present study aimed to explore the immune-enhancing effect of 30% ethanol extract of Protaetia brevitarsis seulensis larvae (PBE) in RAW264.7 macrophage cells. PBE were not cytotoxic to RAW264.7 cells and nitric oxide production increased on PBE treatment in a dose-dependent manner. Furthermore, PBE significantly promoted the expression of immune-related mediators (Inos and COX-2) and cytokines (TNF-α, IL-6, and IL-1β) and the phosphorylation of MAPKs (ERK, p38, and JNK). Taken together, the immune-enhancing effects of 30% ethanol extract of PBE in vitro were identified. These findings can be used as data for the development of edible insect-based functional foods that improve immune function.

• 한국환경과학회지
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• 제31권11호
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• pp.993-997
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• 2022

This study was conducted to evaluate the structure and composition (i.e., pH, moisture, total-N, pathogens, and volatile fatty acids) of broiler and duck manure treated with larvae of three insect larvae, namely, Tenebrio molitor, Protaetia brevitarsis seulensis, and Ptecticus tenebrifer. Hatched Tenebrio molitor (n=300), Protaetia brevitarsis seulensis (n=60), and Ptecticus tenebrifer (n=300) were used in this study; specially, the larvae were divided into six treatments with three replicates. The treatments were as follows: T1: 110 g broiler manure + Tenebrio molitor larvae (n=50), T2: 110 g duck manure + Tenebrio molitor larvae (n=50), T3: 125 g broiler manure + Protaetia brevitarsis seulensis larvae (n=10), T4: 125 g duck manure + Protaetia brevitarsis seulensis larvae (n=10), T5: 105 g broiler manure + Ptecticus tenebrifer larvae (n=50), and T6: 105 g duck manure + Ptecticus tenebrifer larvae (n=50). For all the larval treatments, the following results were observed: The moisture content of the duck manure treat with three insect larvae was higher than that of the broiler manure (p<0.05), whereas broiler manure had a higher pH (p<0.05). In addition, the total nitrogen content of broiler manure was higher than that of duck manure (p<0.05). However, the insect larvae did not significantly affect pathogens (E.coli and Salmonella) and the volatile fatty acids (p>0.05). In conclusion, the use of the three insect larvae to create organic nitrogen compost using poultry manure is feasible.

• International Journal of Industrial Entomology and Biomaterials
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• 제39권2호
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• pp.60-66
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• 2019

This study was carried out to investigate the development and oviposition characteristics of Protaetia brevitarsis fed mulberry fermented sawdust added with mulberry cake. The results of rearing of P. brevitarsis larvae on oak fermented sawdust and mulberry fermented sawdust added with different ratio of mulberry cake, the development periods of larvae feed sawdust with mulberry cake were about 41.6~48.5 days and shortened by 117 or more than that without treatment. The development periods were shorter as the ratio of mulberry cake added increased and there was no difference according to the kind of sawdust. But the weight of the larvae raised in mulberry fermented sawdust added with mulberry cake was heavier than that of the larvae raised in oak fermented sawdust added with mulberry cake. When mass rearing P. brevitarsis larvae in oak fermented sawdust, the addition of 5% mulberry cake was appropriate. Also, the average number of laying eggs of female who fed diet with mulberry cake was 12.3% higher than that of untreated.

• International Journal of Industrial Entomology and Biomaterials
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• 제37권2호
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• pp.90-94
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• 2018

In the present study, we investigated the effects of temperature and photoperiod on oviposition of Protaetia brevitarsis. The effects of long- and short-day cycles on oviposition and egg hatching of P. brevitarsis were investigated at different temperatures. Three male-female pairs were confined to oviposition chambers maintained at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, and $35^{\circ}C$, with 16L:8D and 8L:16D photoperiod. Oviposition was observed at all temperatures. The total number of eggs laid per female was between 46.8 and 110.8, and the optimal temperature for oviposition and fertility was between $20^{\circ}C$ and $30^{\circ}C$. Furthermore, it was difficult for the eggs to hatch at $35^{\circ}C$. Fewer eggs were laid under short photoperiod than under long photoperiod at all temperatures. Hatching success was 93.5% at $20^{\circ}C$, 90.9% at $25^{\circ}C$, 71.5% at $30^{\circ}C$ and 37.3% at $35^{\circ}C$ under long-day(16L:8D) condition and Temperature had a strong effect on the time to hatching. Neither oviposition nor subsequent egg hatching was influenced by photoperiod and temperature. The information obtained will be useful for mass rearing P. brevitarsis.

• 한국환경과학회지
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• 제29권9호
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• pp.935-938
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• 2020

This study investigated the oviposition characteristics of Protaetia brevitarsis seulensis after emergence as adults from the larva collected from three regions in Gangwon-do, Chungcheongnam-do, and Jeju-do. The laying egg method was identified with zone breeding, and the average and cumulative number of eggs laid were measured once a week for seven weeks. The average number of eggs laid peaked until 4 weeks, and subsequently decreased. The source areas with respect to average number and cumulative number of eggs laid were in the order of Jeju > Chungnam > Gangwon. In conclusion, this result suggests a method for continuously improving management and economic feasibility of insect farming by securing genetic diversity, and raising the most productive breeds to select individuals form regions associated with high numbers of laid eggs.

• Animal cells and systems
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• 제3권1호
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• pp.47-52
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• 1999

Change of proteins was confirmed during low temperature acclimation of overwintering larva, and some biochemical characteristics of the induced antifreeze protein-1 (AFP-1) were investigated in Protaetia brevitarsis. As the freezing point depression by the action of induced AFPs, a considerable thermal hysteresis was observed in the haemolymph and in partially purified proteins. AFP-1 was purified from the cold acclimation larvae by ammonium sulfate precipitation ion exchange chromatography, gel permeation chromatography, and electroelution. The purified AFP-1 was determined to be a glycoprotein (approximately 320 kDa, pl 5.8) composed of a single type of subunit (80 kDa). The high contents of hydrophilic amino acids (Asp, Glu, Lys, Asn, Gln, Arg, Ser, Thr) were also confirmed, showing similarity with antifreeze proteins from other insects.

• 약학회지
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• 제50권4호
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• pp.287-292
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• 2006

Protective effects of the water extract of Protaetia brevitarsis larva against $CCl_4-induced$ toxicity were investigated in primary cultures of adult rat hepatocytes. The extract used in these studies contained several minerals, fatty acids and amino acids. Treatment of hepatocyte cultures with the extract provided a significant protection from the increased LDH activity induced by $CCl_4$. The results demonstrated that the extract may have the protective effect against $CCl_4-induced$ toxicity in hepatocyte cultures.

• International Journal of Industrial Entomology and Biomaterials
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• 제46권2호
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• pp.55-59
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• 2023

One of the necessary conditions for the mass production of the edible insect, the white-spotted flower chafer Protaetia brevitarsis seulensis (Kolbe), is to breed a strain with excellent egg-laying ability. To identify external morphological traits related to egg-laying ability, we investigated the effects of the weight, length, and width of female adults on egg production. Correlation and multiple regression analyses revealed a positive correlation between the weight of female adults and egg production. This study suggests that selection of heavier females is a good strategy for breeding strains with superior egg-laying characteristics. The results of this study will serve as important foundational data for future breeding of superior strains.