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A Study of Ni-resistant bacteria isolated from gingival crevicular fluid on the patients wearing Ni-Cr alloy prosthesis (In terms of molecular biological aspects) (니켈-크롬 합금 보철물 주위 치은열구 내에서 발견된 니켈 내성 균주에 관한 분자생물학적 연구)

  • Chae, Young-Ah;Woo, Yi-Hyung;Choi, Boo-Byung;Choi, Dae-Gyun;Lee, Sung-Bok;Kwon, Kung-Rock
    • The Journal of Korean Academy of Prosthodontics
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    • v.37 no.6
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    • pp.741-755
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    • 1999
  • As a material of metal-ceramic prosthesis, nickel as a form of Ni-Cr alloy has been used for many dental prostheses in many cases. However, several problems in use of the alloy have been revealed (ex : tissue stimulation, skin allergy, hypersensitivity, cytotoxicity and carcinogenecity). Little is known about nickel with respect to the relationship between Ni-prosthesis and gaining of Ni-resistance in oral microorganisms. The present study was undertaken to check wheather use of Ni-prosthesis leads to occurrence of Ni-resistant microorganisms. So this study may suggest the possible relationships between the oral microorganisms and nickel-resistance in oral environment. Bacteria were isolated from the gingival crevicular fluid on the pateints wearing Ni-Cr prosthesis. The isolated bacteria were tested for their Ni-resistance in nickel containing media at different concentration from 3mM to 110mM. E. coli HB101 was used as control. The Ni-resistant bacteria were isolated and biochemically identified. The Ni-resistant bacteria were tested several bio-chemical, molecular-biological tests. Performed tests were ; measuring the growth curve, antibiotic test, growth ability test in liquid media, isolation of the chromosome and plasmid, digestion of DNA by restriction enzyme, electrophoresis of chromosome and plasmid DNA, identification of Ni-resistant genes by the DNA hybridization. The results were as follows: 1) The bacteria isolated from gingival crevicular fluid on the patients wearing Ni-Cr alloy pros-thesis showed nickel-resistance. 2) The isolated microorganisms grew at nickel containing media of high concentrations (60mM-110mM). 3) Based on the biochemical tests, the isolated microorganisms were identified as Enterococcus faecalis(13 cases), Klebsiella pneumoniae(1 case) and Enterobacter gergeviae(1 case). 4) Enterococcus faecalis expressed not only nickel resistance but also the multi-drug resistance to several antibiotics ; chloramphenicol, kanamicin, streptomycin, lincomycin, clindamycin. However, all strain showed the sensitivity against the tetracycline. 5) DNA hybridization result suggest that there is no homology between the previousely known gene of nickel resistance in Klebsiella pneumoniae and chromosomal DNA of Enterococcus faecalis.

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Market oriented Typology and Development Directions of Conventional Markets (재래시장의 시장 지향적 분류에 따른 발전방향)

  • Kim, Ju-Yeong;Park, Jeong-Eun
    • Journal of Distribution Research
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    • v.16 no.5
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    • pp.63-84
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    • 2011
  • There are about 1,500 small or large traditional markets in Korea. Those conventional markets have been struggling for survival in rapidly changing market environment with highly intensified competition by accelerating the entrance of the Large-scale Discount Store and Super Supermarket which are managed and supported by Large companies like Samsung and Lotte. The loosing power of conventional markets have threatened the local merchants' survival and have influenced local economics falling down. To create better value for the customers who have more preference for department store and discount stores than conventional markets, conventional markets should have market orientation, which has three sub dimensions; customer orientation, competitor orientation and inter-functional coordinations. First objective of study is to find the appropriate roles of conventional markets in local areas. Second is to find out the market orientation functions for conventional markets to be survival in local markets in the long terms. Finally, this study will suggest some policies for the appropriate types of successful conventional markets considering various local situations. To achieve those objectives, we did the following procedure. First, we review the previous reports and studies of policies for conventional markets and local economy development, and propose some pros and cons of the previous policies. Secondly, we develop a market orientation framework of conventional markets, in which we suggest the developmental types and functions of market-orientation of conventional markets in accordance with the characteristics of local market and economic conditions of local areas. Then we formulate and discuss the study's contributions, managerial implications, and directions for future policy development. Especially for the policy development, we suggest the efficiency and economical effects of policy, human being development and management system, and the role of government and protection of local merchants in the development process.

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Development of Physician Coaching Model for Improvement of Patient-Doctor Communication (환자-의사 커뮤니케이션 개선을 위한 의사코칭 모델 개발)

  • Na, Hyun Sook;Kwon, Young Dae;Noh, Jin-Won
    • The Journal of the Korea Contents Association
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    • v.13 no.2
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    • pp.331-340
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    • 2013
  • Recently there is a heated debate going on regarding the patient-doctor communication in the medical schools and medical service sector. Patient-doctor communication is an interactive communication made during the consultation session which is known to bring positive effect to both the patient and the doctor. Through this research, a doctor coaching model was developed by combining a method that would help the patient and doctor communicate better by increasing the doctor's communication skill and a coaching mechanism. Through the research, the doctor coaching model consists of 5 levels. First is the 'relationship creation' which would cause the doctor's interest and expectations toward coaching mechanism. Second is 'recognition of change' and this would cause to understand the problem and pros of the doctor's communication with the patient and set a direction regarding the coaching. Third is 'understanding the perspective' and this would lead the doctor to think from the patient's perspective. Fourth is 'increasing problem solving and communication skills' and this would set specific terms as to how the doctor can improve his communication skills. Fifth is 'goal setting and support' where goal regarding the improvements can be set and agreement regarding the ways to maintain and strengthen the advantage can be made. The developed doctor coaching model is most meaningful in a way that it has first adapted a coaching mechanism to improve patient-doctor communication. Also in cases where such will be utilized in the future medical service sector, it is expected to affect greatly the doctor's communication skill and patient sympathizing skills. Hereby it will contribute in increasing the patient's treatment satisfaction.

A comparative study of bite force associated with remaining bone level in anterior and premolar teeth at periodontal maintenance phase (유지관리기 치주환자의 전치 및 소구치에서 잔존 치조골량에 따른 교합력의 비교 연구)

  • Song, Gyu-Won;Yim, Sung-Bin;Chung, Chin-Hyung
    • Journal of Periodontal and Implant Science
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    • v.32 no.3
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    • pp.643-653
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    • 2002
  • Periodontal supporting tissue goes through destruction by chronic inflammatory periodontal disease as two aspect. One is qualitive aspect such as alteration of periodontium, the other is quantitative aspect such as alveolar bone loss. According to many authors, PDL is responsible for biting force, and there are two mean. for measuring PDL's function - mobility test and biting force test. This study was conducted to compare the biting force with remaining bone level, that is, quantitative aspect of periodontium, in anterior and premolar teeth at periodontal maintenance phase. 17 patients on periodontal maintenance phase during 6 months at minimum were selected for this study. For the same condition, 4 anterior, canine, premolar teeth were tested by MPM-3000 bite-force register at the same time(a.m.l0-12), the same position, the same posture, by the same examiner. Patients of TMI), ill-fitting pros thesis, general disease, malocclusion and the teeth of TFO, absence of opposing teeth, malposition were excluded. Remaining bone level was measured on the panorama X-ray film through 5 level from 1mm below CEJ to root apex. Teeth were examined twice, and bigger one was selected. If the values showed large difference. examinatin was re-done and the mean was selected. The results were as follows ; 1. In the 4 anterior group, as the remaining bone is decreased, the average of maximal biting force is decreased. Especially, at 3/5 bone level, maximal biting force is decreased significantly(p<0.01). 2. In the canine group, as the remaining bone is decreased, the average of maximal biting force is decreased. Especially, at 2/5 bone level, maximal biting force is decreased significantly(p<0.01). 3. In the premolar group, as the remaining bone is decreased, the average of maximal biting force is decreased. Especially, at 3/5 bone level, maximal biting force is decreased significantly(p<0.05). From the results of this study, clinicians could utilize these efficiently when they have to determine the proper restorative materials, time for tooth extraction, treatment plan, prognosis.

Multicast Routing Protocol for Multimedia Applications (멀티미디어 응용을 위한 멀티캐스트 라우팅 프로토콜)

  • Lee, Kwang-Il;Cho, Pyoung-Dong;Kim, Kyoung-Il;Kim, Sang-Ha
    • The Transactions of the Korea Information Processing Society
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    • v.5 no.5
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    • pp.1274-1284
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    • 1998
  • Multicasting plays an important role in providing multimedia services like audio and video conferencing and has been actively studied for many years. The study of multicasting has been mainly focused on finding a low-cost tree on the assumption that every edge of the tree has enough link capacity to be allocated to the amound of bandwidth required by a service. In some cases, however, a multicasting tree whose some edges do not have enough bandwidth to be allocated may exist. A typical example of this case is to multicast among the group users located in the different sub-networks in the interconnected networks composed of the high bandwidth networks such as B-ISDN and the low bandwidth networks such as PSTN and PSDN. This paper proposes a novel multicasting protocol for providing multimedia services in a wide area network interconnected by multiple heterogeneous networks. First, the paper discusses the inherent limitation of present multicasting protocols for the case. Second, se introduce a new concept, multiple QoS's for a service, which extends the implicit assumption that only one QoS is assigned to a service in order to overcome this limitation. Third, our multicasting protocol is designed based on the current Internet protocol stacks. Last, we perform simulations for possible scenarios to provide multimedia services in the network and analyze their pros and cons.

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A Critical Analysis on Korea's Tidelands Policy : From a Sustainable Development Point of View (한국의 갯벌정책에 대한 비판적 소고: 지속가능한 발전의 관점에서)

  • Moon, Seogwoong
    • Environmental and Resource Economics Review
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    • v.16 no.3
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    • pp.575-605
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    • 2007
  • Korea's tidelands policy IS examined and criticized in the paper. Korea's major tidelands in the west coast might disappear within a decade. The anachronistic development IS driven by the political interest group, forming coalition for reclamation and busily fulfilling its own profits under the facade of public good and regional development. But all regions of the world have been gradually scrapping the massive reclamation projects since the 1970's and active movements to reverse the reclamation and restore the nature are on the rise. In 1978, the U.S. Supreme Court decided to suspend the construction of the dam and to protect the snail darter despite that over 100 million dollars had been injected. This court ruling became famous and caused the American public to change their perception about the environment. In the Netherlands, following a prolonged discussion on pros and cons of reclamation, open type seawall was adopted to strike a balance among the available alternatives. Japan's Ministry of Environment was praised for forming the National Wetland Committee in order to designate 20 new Ramsar sites by 2008, with an objective to reach 33 designated Ramsar sites. Away from the large-scale reclamation projects, Korea has to move towards smaller-scale projects focusing on the knowledege intensive and circular economy society that are more in harmony with the environment.

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Usefulness estimating of Time of flight(TOF) during Carotid angio inspection including Aortic arch (Aortic arch를 포함한 Carotid angio 검사 시 Time of flight(TOF)의 유용성 평가)

  • Yoo, Yeong-Jun
    • Korean Journal of Digital Imaging in Medicine
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    • v.15 no.1
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    • pp.1-7
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    • 2013
  • Purpose : The Carotid Angio inspection including Aortic arch applied to wide area is conducted as the Contrast Enhance MR Angiography(CEA) which is using a contrast medium. However it is a burden not only for someone such as infants, pregnant women and patients suffering from kidney failure but continuous use of contrast medium also can be a burden for patients who has been taken follow up inspection since diagnose lesion already. The purpose of this study is to estimate a usefulness of the Time of Flight (TOF) by comparing with CEA. Materials and methods : 10 patients with an average age of 58 (from 45 to 75) who had MRA inspection in our hospital were studied using 3.0 Tesla Aachieva (Philips, Netherland) MRI system and Sense Neuro-Vascular 16 Channels Coil. The same patient was inspected both TOF and CEA simultaneously. The TOF inspection included from Aortic arch to Willis Circle by connecting 3 TOF stacks and so did CEA inspection. The quantitative analysis was conducted through signal to noise ratio(SNR) and contrast to noise ratio(CNR) with soft tissue by setting up an area of interest on CCA bifurcatoin, ICA, ECA, MCA and VA concerning obtained image. In case of qualitative analysis, 3 radiological technologists and 1 radiologist evaluated 4 items (1: Visibility of the blood vessel, 2: Image distortion measure, 3: Overlapping measure with vein, 4: Peripheral blood vessel description measure) into five points scale (1: Very bad, 2: Bad, 3: Normal 4: Good, 5: Very good). Results : Results for the quantitative analysis was obtained by calculating the average of 5 ROIs in case of SNR and CNR separately. Results of SNR, TOF were generally measured higher than CEA (In case of TOF were 166.1, 205.2, 154.39, 172.23, 161.95, and CEA were 92.05, 95.43, 84.76, 73.69, 88.3). But according to the result of CNR, both TOF and CEA were measured similarly as 67.62, 106.71, 55.9, 73.74, 63.46 for TOF and 67.82, 71.19, 60.52, 49.45, 64.07 for CEA. Throughout every results of each ROI, SNR showed statistically meaningful consequence (0.050.05). In case of qualitative analysis the average of each evaluated item was 4.2points and 4.28points in the item1, 2.93points and 4.55points in the item2, 4.6points and 3.13points in the item3, 2.88points and 4.6points in the item4. According to the results TOF was measured higher in the item3 while in the item2 and item4 CEA was higher and in case of the item1, both CEA and TOF were similar. To sum up statistically meaningful results (p<0.05) were shown in the item2, item3 and item4 but not in the item1 (p>0.05). Conclusions : Both TOF and CEA are complementary because each inspection has pros and cons, but when inspect wide area including Aortic arch normally CEA is conducted. But TOF inspection also can be considered as alternative in terms of patients who has difficulty in the contrast medium such as infants, pregnant women and patients suffering from kidney failure and patients during follow up.

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OCCLUSAL FORCE AND ORAL TACTILE SENSIBILITY MEASURED IN PARTIALLY EDENTULOUS PATIENTS WITH BR NEMARK IMPLANTS AND NATURAL TEETH: A CLINICAL STUDY (기능 노출 기간에 따른 골 유착성 임플란트의 촉각 감지도와 최대 교합력의 변화)

  • Jung, Bock-Young;Jeon, Young-Sik;Han, Dong-Hoo
    • The Journal of Korean Academy of Prosthodontics
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    • v.37 no.1
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    • pp.23-41
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    • 1999
  • During the previous several decades the osseointegrated implants have been considered as the most ideal therapy for the fully edentulous or partially edentulous patients. Although the research on the biocompatibility of osseointegration, the oral tactile function, and the histo-neurologic study had been performed, the change of the oral tactile sensibility and maximal occlusal force according to the postinsertion time of implant prostheses has not been studied. The purpose of this study was to compare the oral tactile sensibility and maximal occlusal force of implant pros-theses to natural teeth according to the specific postinsertion time intervals. The fifty seven patients treated with $Br{\aa}nemark$ implants during the recent seven years were involved in this study. The oral tactile sensibility and maximal occlusal force were measured according to the postinsertion time (${\leqq}6\;months,\;{\leqq}12\;{\leqq}\;18\;months\;{\leqq}\;24\;months,\;and\;{\geqq}20\;months$) 1. The passive tactile threshold of implants was higher than 6-7 times when comparing to natural teeth(P<0.05) 2. The absolute pressure threshold in horizontal and vertical direction for the passive tactile sensibility had decreased significantly in the post insertion time 13-18 months group and had increased significantly in the post insertion time over 25 months group (P<0.05) 3. 50% limit thickness and 100% absolute thickness for the active tactile sensibility had decreased significantly in the postinsertion time 7-12 months group ($AT_{50}:P<0.005,\;AT_{100}:P<0.05$) 4. The maximal occlusal force of the implants prostheses had decreased when comparing with natural teeth in the postinsertion time 1-6 months group(P<0.05) and after that there was no significant difference between the implant prostheses and natural teeth(P<0.05) 5. Maximal occlusal force according to the postinsertion time had increased significantly in the 13-18 months group (P<0.05) after that there was no significant difference. 6. There was positive correlation between age and oral tactile sensibility tested in horizontal and vertical direction ($P_{H:r}=0.39,\;P_{v:r}=0.41$) and there was no correlation between age and maximal occlusal force, age and active tactile sensibility 7. According to the results or the questionnaire, 76.6% of the patients were satisfied with the function and 96.7% of the patients were satisfied with the sensibility. In conclusion oral tactile sensibility and maximal occlusal force of implants have changed with the postinsertion time. Oral tactile function of implants was lower than that of the natural teeth, but for the maximal occlusal force there was no difference between implants and natural teeth.

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The Survey of High School Students' Concern Levels on Decision-making Problems based on Biology (생물영역에서 고등학생의 의사결정 문제에 대한 관심도 조사)

  • Hong, Jung-Lim;Chang, Nam-Kee
    • Journal of The Korean Association For Science Education
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    • v.19 no.1
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    • pp.1-7
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    • 1999
  • The purpose of this study was to survey the high school students' concern levels on decision-making problems based on biology, This study is a exploratory research to develop teaching strategies and curriculum of the common science for the enhancement of the students' decision-making ability in problems involved biological knowledges. The survey subjects were 101 first graders of the high school in Seoul area. The survey instrument developed was 5-point scale of Likert type consisted of 24 question items. The survey results showed that the concern level on selection of method for learning was the highest. The concern levels on selection of method for sports or diet which are the individual faced problems were high, and concern levels on the problems social issued such as decisions about standard for noise, pros and cons of approval for brain death or artificial abortion were high, too. The students' concern levels on problems individual context were significantly higher than concern levels on problems social context (p<0.01). The males' concern levels were higher than females' on 'AIDS', 'incinerator for rubbish', 'atomic power plant', 'protection policy for decreasing species', 'standard for noise' (p<0.05), And the males' concern levels were higher than females' on domain of 'ecosystem and environment pollution'. But the females had higher concern levels than males on 'diet' and 'surrogate mother' (p<0.05). The analyzed results were discussed in respects of implication for teaching strategies and curriculum.

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Bioconversion Strategy in Conversion of Lignocellulosic Biomass upon Various Pretreatment Methods using Sulfuric Acid and Aqueous Ammonia (황산과 암모니아를 이용한 목질계 바이오매스의 전처리 공정에 따른 당화 및 발효공정 전략)

  • Cayetano, Roent Dune;Kim, Tae Hyun;Um, Byung-Hwan
    • Korean Chemical Engineering Research
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    • v.52 no.1
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    • pp.45-51
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    • 2014
  • This is to study the effects of various pretreatment methods of agricultural residue, corn stover, and to compare the feature and pros and cons of each method including dilute sulfuric acid (DSA), soaking in aqueous ammonia (SAA), and ammonia recycle percolation (ARP). In order to convert corn stover to ethanol, various pretreatments followed by simultaneous saccharification and co-fermentation (SSCF) were tested and evaluated in terms of ethanol yield. With 3%, w/w of glucan loading using ARP-, DSA-, and SAA-treated solids, SSCFs using recombinant E. coli strain (ATCC$^{(R)}$ 55124) with commercial enzymes (15 FPU of Spezyme CP/g-glucan and 30 CBU/g-glucan enzyme loading) were tested. In the SSCF tests, 87, 90, and 78% of theoretical maximum ethanol yield were observed using ARP-, DSA-, and SAA-treated solids, respectively, which were 69, 58, and 74% on the basis of total carbohydrates (glucan + xylan) in the untreated corn stover. Ethanol yield of SAA-treated solid was higher than those of ARP- and DSA-treated solids. In addition, SSCF test using treated solids plus pretreated hydrolysate indicated that the DSA-treated hydrolysate showed the strongest inhibition effect on the KO11 strain, whereas the ARP-treated hydrolysate was found to have the second strongest inhibition effect. Bioconversion scheme using SAA pretreatment and SSCF can make the downstream process simple, which is suggested to produce ethanol economically because utilization of hemicellulose in the hydrolysate is not necessary.