• Bulletin of the Korean Chemical Society
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• 제24권7호
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• pp.943-947
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• 2003

We have studied the migration behavior of single-stranded DNA using capillary gel electrophoresis under various conditions. It was found that optimum electric fields should be less than 150 V/cm for the good tradeoff between the separation time and the resolution. It seems that the gel matrix with the combination of different polymer average molecular weights is important to extend the maximum readable DNA bases. The total gel concentration less than 3.1% in the mixed gel system showed good separation efficiency up to 600 bases. The best result was obtained with the poy(ethylene)oxide (PEO) gel concentration of 1.2% of Mr 8,000,000 and 1.8% of Mr 600,000. We observed that the capillary length between 50 cm to 100 cm (effective length) should be employed for the optimization between the total DNA migration time and the maximum readable length. A trizma base-boric acid-ethlyenediaminetetraacetic acid (EDTA) (TBE) buffer was commonly used for DNA sequencing, but we found that 3-[tris(hydroxymethyl)methyl amino]-1-propane sulfonic acid (TAPS) buffer worked as well for the single-stranded DNA separation. Especially, TAPS buffer showed a good resolution for very short DNA bases (1 to 30 bases).

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2006년도 춘계학술발표대회
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• pp.669-672
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• 2006

고온가스냉각 원자로에서는 고온 안정성 및 핵분열생성물 차단 성능이 우수한 TRISO(tri-tsotropic) 핵연료를 사용하고 있다. TRISO 핵연료 입자는 직경이 약 1 mm인 구 형태로 입자의 중심에는 직경 $0.5{\mu}m$의 핵연료 커널(kernel)이 포함되며 커널 외곽을 코팅 층이 에워싸고 있다. 이 코팅 층은 완충(buffer) PyC(pyrolytic carbon) 층, 내부 PyC 층, SiC 층, 그리고 외부 PyC 층으로 구성되어 있다. 각 코팅 층의 두께는 수십${\sim}$백 ${\mu}m$ 범위이며, 본 연구에서는 각 코팅 층의 두께를 비파괴적으로 측정하기 위하여 마이크로포커스 X-선 발생장치와 고해상도 X-선 평판(flat panel) 검출기로 구성된 정밀한 X-선 래디오그래피 장치를 구성하고, $UO_2$ 핵물질 대신에 $ZrO_2$를 커널로 사용한 모의 TRISO 핵연료 입자에 대한 래디오그래피 영상을 획득한 후 디지털 영상처리기술을 이용하여 코팅 층 사이의 경계선이 구분 가능하도록 영상을 개선하고 디지털 영상처리 알고리즘을 개발하여 코팅 층의 두께를 측정하였다.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2005년도 추계학술발표대회 및 정기총회
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• pp.713-716
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• 2005

차세대 원자로로 부각되고 있는 고온가스냉각 원자로에서는 고온 안정성 및 핵분열생성물 차단 성능이 우수한 TRISO(Tri-Isotropic) 핵연료를 사용하고 있다. TRISO 핵연료 입자는 직경이 약 1mm인 구 형태로 입자의 중심에는 직경 $0.35^{\sim}0.6\;{\mu}m$의 핵연료 입자가 포함되며 입자 외곽을 코팅 층이 에워 싸고 있다. 이 코팅층은 완충(buffer) PyC 층, 내부 PyC 층, 외부 PyC 층으로 구성되어 있다. 각 코팅 층의 두께를 수십$^{\sim}$백 ${\mu}m$ 범위이며 사양으로 정해져 있어 핵연료 입자 제조 후 사양을 만족하는지를 검사해야 한다. 본 연구에서는 TRISO 핵연료 입자 정보를 컴퓨터로 생성하고 가상의 X-선 래디오그래피 방법을 이용하여 투시 영상을 구성한 후 Filtered Backprojection 기법을 이용하여 단면 영상을 재구성하고 이 단면 영상을 이용하여 코팅 층의 두께를 정밀하게 측정하기 위한 모의 실험을 수행하였다. 경계선이 불명확한 투시영상이 아닌 경계선이 명확한 재구성 단면 영상을 이용하여 코팅 층의 두께를 약 2.3% 이내의 오차율로 정밀하게 측정하였다.

• Journal of the Korean Institute of Landscape Architecture International Edition
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• 제2호
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• pp.120-129
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• 2004

Urban stream restoration projects can improve water quality, wildlife habitats, urban landscape, outdoor recreation spaces, and urban microclimate. The objectives of this research were to investigate temperature cooling effect of urban streams by using satellite imagery, to evaluate environmental variables related to stream cooling effect, and to estimate the cooling effect of the Cheonggye stream restoration project of Seoul, Korea. Findings of this research can be summarized as follows. First, a method of estimating temperature distribution around urban streams by using satellite imagery was developed. Scatter plots of distance from stream edges and average temperature obtained through multiple buffering were used for the estimation. Second, urban temperature cooling effect of streams was estimated by comparing background temperature and temperature of each buffer zone. Third, environmental factors affecting stream cooling effect were also identified. Fourth, the temperature cooling effect of the restoration project was estimated based on three scenarios. An estimated cooling effect based on the average cooling effect of existing tributaries showed the most significant effect; $2.0^{\circ}C$ lower than the present level at the edge of the renovated stream. It was estimated that the temperature of the same area would be $1.4^{\circ}C$ cooler than the present level if the cooling effect of the Yangjaechun was used as the bench mark But the effect would be $1.2^{\circ}C$ lower than the present level if environmental variables related to the temperature cooling effect of urban streams were used as the bench mark.

• 한국터널지하공간학회 논문집
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• 제14권6호
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• pp.575-593
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• 2012

본 연구는 국제공동연구인 SKB Task 8에 대한 간단한 소개와 더불어, Task 8a 모델문제를 중심으로 유한차분과 유한체적법의 복합적 해석코드(integrated finite difference method)인 TOUGH2를 이용하여 벤토나이트 완충재와 현장암반의 수리학적 거동특성을 살펴보았다. 이를 위해 벤토나이트-암반의 상호작용을 중심으로 현장암반의 절리유무에 따른 완충재 내의 압력분포 변화 및 재포화 특성을 분석하였고, 벤토나이트 고유 특성인 높은 모세관 압력과 현장암반 내 절리영향을 TOUGH2 코드를 통해 성공적으로 구현하였다. 연구결과, 현장암반 내 절리를 고려함으로써 완충재의 포화속도는 약 2.5~12배 가량 차이가 발생하였으며, 무결암 및 절리암반 모두 완충재의 하단부가 상단부에 비해 포화속도가 상대적으로 빠르게 진행되었음을 확인할 수 있었다. 본 연구결과는 고준위폐기물 처분공 내의 완충재 설계와 관련하여, 완충재 포화 시기예측 및 최적 소요두께 결정 등에 중요한 자료로 활용될 수 있을 것으로 예상된다.

• 한국항공우주학회지
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• 제46권6호
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• pp.513-518
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• 2018

KUH-1에 적용되는 질소충전트레일러는 항공기의 주/전륜 완충기, 테일범퍼완충기, 브레이크 축압기, 타이어 등에 질소를 충전하는 장비이다. 명칭에서 알 수 있는 것과 같이 질소충전트레일러는 질소 충전과 더불어 이동성을 가지고 있는 장비이다. 기존 미국의 Tronair사에서 독점 공급하여 국내에서는 수입에 의존하던 품목이며, 본 국산화 개발품은 도입품의 성능을 만족할 뿐만 아니라 운용 시 도입품보다 용이하도록 설계를 추진하였다. 제15-4차 민관공동투자사업으로 선정되어 개발이 진행되었으며, 민관투자기업인 한국항공우주산업과 국방기술품질원의 개발관리 지원하에 개발요구도 분석 및 시험평가가 수행되었으며, 2017년 5월 군사용 적합 판정 결과 승인을 받았다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권2호
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• pp.165-171
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• 2003

Sixty semen ejaculates collected at weekly interval from four Murrah Buffalo bulls over a period of seven months (Nov.1999 to May 2000) were used in the present study. Three buffer medium (sodium citrate, TES and Tris) were used for soaking of sephadex. Three grades of sephadex (G-15,G-100, and G-200) were used for preparation of columns. Columns of three different height (one, two and three cm) were used for separation of semen. Twenty semen ejaculates were used in each project. In the first experiment each semen ejaculates was divided into four parts. One part was kept as control and other three parts were passed thought one cm column of sephadex G-15 prepared in three different buffers. There was significant (p<0.05) increase in percent progressive sperm motility and percent live spermatozoa and decrease in percent abnormal spermatozoa and percent spermatozoa with damaged acrosome as well as sperm numbers after filtration through all the three columns. Sperm quality obtained in the filtrate of column prepared in Tris buffer was better in comparison to other two buffers. So the Tris buffer was used in the second trial. Twenty semen ejaculates were used in this experiment. Each semen ejaculate was divided into four parts. One part was kept as control (non-filtered) and other three parts were passed through columns of different grade of sephadex (G-15, G-100 and G-200). Progressive sperm motility and live sperm percentage improved significantly while decline in percent abnormal spermatozoa and percent spermatozoa with damaged acrosome and sperm concentration was observed after filtration through all the columns as compared to control (non-filtered) semen. Since post filtration quality of semen was better in the sephadex G-100 column, therefore it was selected for the next experiment. In third experiment, Tris buffer and sephadex G-100 were used for preparing columns of different height (one, two and three cm) and twenty semen ejaculates were filtered. The quality characteristics of semen (percent progressive sperm motility, percent live spermatozoa and sperm concentration) after filtration through one cm column were significantly (p<0.05) higher than after filtration through columns of two and three cm height. However non -significant (p>0.05) difference due to height of columns was observed for percent abnormal and percent damaged acrosome but 1 cm column comparatively gave better result than of 2 and 3 cm column height.

• 비파괴검사학회지
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• 제26권2호
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• pp.69-76
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• 2006

차세대 원자로로 부각되고 있는 고온가스로에서는 윈자로에서는 고온 안정성 및 핵분열생성물 차단 성능이 우수한 TRISO(tri-isotropic) 핵연료를 사용하고 있다. TRISO 핵연료 입자는 직경이 약 1mm인 구 형태로 입자의 중심에는 직경 0.5mm의 핵연료 커널(kernel)이 포함되며 커널 외곽을 코팅 층이 에워싸고 있다. 이 코팅 층은 완충(buffer) PyC(pyrolytic carbon)층, 내부 PyC층, SiC층, 그리고 외부 PyC층으로 구성되어 있다. 각 코팅 층의 두께는 수십-백${\mu}m$ 범위이고 사양으로 정해져 있으며, 본 연구에서는 각 코팅 층의 두께를 비파괴적으로 측정하기 위하여 마이크로포커스 X-선 발생장치와 고해상도 X-선 평판(flat panel) 검출기초 구성된 정밀한 X-선 래디오그래피 장치를 개발하였다. 개발된 마이크로 X-선 래디오그래피 장치를 이용하여 $UO_2$ 핵물질 $ZrO_2$를 커널로 사용한 모의 TRISO 핵연료 입사에 대한 투과 영상을 획득한 후 디지털 영상처리 기술을 이용하여 코팅 층 사이의 경계선이 구분 가능하도록 영상을 개선하고 디지털 영상처리 알고리듬을 개발하여 코팅 층의 두께를 파동으로 측정하였다.

• 한국환경복원기술학회지
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• 제19권2호
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• pp.109-119
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• 2016

In South Korea the need of sustainably managing development on forest lands has required to develop a new approach to estimating environmental impacts on forest surrounding development sites in a scientific manner. As for forest-related development, two types of development were selected: golf courses and industrial complexes. Using Fragstats 4.2, the fragmentation effects and patterns of each type by forest area within project sites and buffer zones ranging from the outside of project sites up to 2,000 meters were analyzed. As a result, golf courses were strongly related to a group of fragmentation indexes: CA, NP, PD, TE, LSI, TCA, NDCA and CONNECT, whereas industrial complexes were associated with CA, NP, PD, TE, LSI and CONNECT. Among them, NP, LSI, TCA and NDCA of golf courses were considered as representative indexes reflecting the average impact ranges of each sub-group by forest area, and focussing on the size of core areas. In the case of industrial complexes, PD, TE and LSI were the representatives, vulnerable to the composition of given landscape. For two case studies, one for golf courses and the other for industrial complexes, they showed there existed a difference between the average of a group and the individual results. Therefore, to minimize the variations in impact range within a group, it is needed to analyze more individual cases. This study proved there was a distinction between project types in terms of the range of environmental impact. To effectively and comprehensively manage forest development, further research on analyzing other development types related to forests with more cases is needed.

• 농촌계획
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• 제28권1호
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• pp.17-26
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• 2022

It is necessary to prepare a stable production base in advance for a change in the global grain market, and it is required to prepare comprehensive countermeasures such as securing technical skills and cultivation technology. Therefore, Korea, which relies on imports of major grains other than rice, could be exposed to a food crisis at any time unless the self-sufficiency rate of grains is improved. In order to respond to this new food crisis, it is necessary to find ways to efficiently utilize rice fields to increase the domestic grain self-sufficiency rate. From this point of view, interest and demand for the generalization of farmland that can be used as paddy fields and returned to paddy fields are increasing, and related research is also being continuously performed. In order to select a multipurpose farmland project site, this study extracted farmland containing 10% or more purchased and stockpiled farmland through spatial analysis (buffer, dissolve, intersect, etc.), and finally presented areas subject to multipurpose farmland projects. The target site for the multipurpose farmland project was finally selected by integrating data onto a point-by-point basis so that the current status of farmland purchased and stockpiled, Farm Manager Registration Records, and the Korean Soil Information System data (drainage classes, surface soil texture, field-suitability classification, etc.) can be used in combination. There are 175 areas where the multipurpose farmland is possible. Incheon 2, Gyeongbuk 40, Gangwon 2, Chungbuk 7, Chungnam 48, Jeonbuk 34, Jeonnam 19, Gyeongbuk 15, Gyeongnam 8. Chungcheongnam-do has the most target site for the multipurpose farmland project, and Gangwon-do is the least. It is expected to contribute to new commercialization and business expansion by deriving business areas by identifying the scale of the farmland multipurpose farmland project using Farm Manger Registration Records and spatial data.