• Journal of Yeungnam Medical Science
• /
• 제23권1호
• /
• pp.62-70
• /
• 2006

전립선비대증은 노인 남성에서 흔히 유발되는 질환이며, 노화가 진행될 수록 빈도가 높아지는 특징을 가진다. 이 질환의 원인은 전립선 기질세포의 과도한 증식으로 유발된다고 알려져 있지만 그 자세한 기전에 대해서는 잘 알려져 있지 않다. 전립선비대증에서 progesterone 수용체 양성 세포가 다른 전립선 종양에 비해서 많고, progesterone은 testosterone에서 DHT로 전환되는 것을 감소시키는 역할을 가진다고 알려졌다. 또한 남성 전립선 평활근의 과증식에 의한 질환이므로 평활근 세포의 증식과 관련성이 있다고 보고된 COX-2의 전립선비대증에 대한 영향에 대한 연구가 필요하다. 전립선 기질세포에 progesterone을 3일간 투여하여 배양한 경우 기질세포 증식은 차이가 없었다. Progesterone을 단독 또는 DHT와 같이 투여한 기질세포에서 남성호르몬 수용체 mRNA 발현은 비처리군과 비교하여 유의한 차이가 없었다. 또한 progesterone과 DHT 동시 투여에 의한 COX-2 mRNA 발현에도 차이가 없었다. 그러나 progesterone에 의한 남성호르몬 수용체와 COX-2 단백 발현에서는 대조군과 비교하여 유의하게 감소시켰다. 이상의 결과는 progesterone은 남성호르몬 수용체에 대해 전사 후 반응 (post-transcriptional response)에 효과를 나타내어 남성호르몬 수용체 발현을 감소시키는 작용은 가지며, COX-2 발현 억제효과를 나타내므로 전립선비대증의 치료에 이용될 수 있을 것으로 사료된다.

• Clinical and Experimental Reproductive Medicine
• /
• 제19권1호
• /
• pp.9-14
• /
• 1992

Platelet activating factor(PAF) has been reported to play a significant role in ovulation, establishment and maintnance of early pregnancy. The object of this study was to investigate the influence of PAF on progesterone secretion in rabbit by measurement of pheripheral blood concentration of progesterone. PAF had no effect on progesterone secretion and did not induce decidual reaction in nonovulatory rabbit. But 8th day of hCG induced pesudopregnant rabbit, PAF significantly increase progesterone secretion. Progesterone level was significantly increased at 0.5 and 4 hours after treatment with $10^{-8}$ M PAF on days 2, 4, 6, 8 of gestation as compared than those treated with normal saline. When PAF was injected 2 days after coitus, progesterone levels on days 4, 6, 10, 14 of gestation was significantly increased than those with saline injected group. These results suggest that PAF increase progesterone secretion from the hCG-primed ovary and during pregnancy in rabbit.

• 대한수의학회지
• /
• 제31권2호
• /
• pp.223-228
• /
• 1991

Most progesterone enzyme immunoassays(EIA) are used liquid-phase double-antibody or single-antibody seperation. These methods consume considerable time and reagents because of the requirements for several washing and centrifugation steps involving the reactants. Because of these several problems, we were prompted to develop an effective enzyme-linked immunosorbent assay(ELISA) system that would be equal or superior to RIA for assay of progesterone. The results were obtained as follows. 1. Cross reaction of the progesterone antiserum with other steroids determined was shown with progesterone(100%), $11{\alpha}$-deoxycorti-costerone(2.271%), but the other steroids were shown below 0.9%. 2. Standard curve for progesterone ELISA was shown available difference according to progesterone concentration from 0 to 1,000pg/ml. 3. The lower limit of sensitivity was 0.2pg/well 4. Progesterone concentration was 1.6ng/ml for before parturition, and that was below 0.5ng/ml for after parturition. This development enzyme-linked immunosorbent assay for progesterone can be detected pregnancy diagnosis in cattle, and also applicable 10 research on physiological function including such as reproductive disorders.

• Asian-Australasian Journal of Animal Sciences
• /
• 제11권3호
• /
• pp.300-306
• /
• 1998

An experiment was conducted to investigate correlations of litter size and average serum progesterone concentrations during pregnancy with mammary gland growth and development at parturition. Twenty ewes (5, 9, 4, and 2 ewes carrying 0, 1, 2, and 3 lambs, respectively) were used to measure weekly serum progesterone concentration during pregnancy. At parturition, the experimental ewes were slaughtered for determination of mammary gland growth and development at parturition (mammary dry fat-free tissue [DFFT], DNA, RNA, collagen, protein, and glycogen). Correlation of mammary DFFT with litter size and averages serum progesterone concentrations were 0.75 and 0.72, respectively. Litter size or maternal serum progesterone concentrations did not correlate with the mammary DNA concentration. However, litter size or maternal serum progesterone concentrations positively correlated (p < 0.01) with the mammary RNA and protein concentrations, but negatively correlated with the mammary collagen (p < 0.01) and. glycogen (p < 0.05) concentrations. Litter size or maternal serum progesterone positively correlated (p < 0.01) with the total mammary DNA, RNA, collagen, protein and glycogen contents. These results implied that the increased concentrations of progesterone with the increased litter size during pregnancy improved mammary gland growth and development at parturition.

• 대한수의학회지
• /
• 제34권4호
• /
• pp.867-872
• /
• 1994

Progesterone catabolizing enzyme, the enzyme $20{\alpha}$-hydroxysteroid dehydrgenase($20{\alpha}$-HSD) is pivotal in the regulation of ovarian function in rodents, which catabolizes progesterone into biologically inactive $20{\alpha}$-hydroxypregn-4-en-3-one($20{\alpha}$-OHP). In this study was carried out the influence of $20{\alpha}$-HSD activity of ovarian function, we investigated changes in ovarian cytosol $20{\alpha}$-HSD activity and serume progesterone concentration during the estrous cycles and pregnancy in rat. During the estrous cycles, the $20{\alpha}$-HSD activities were highest on the progestrous, but serum progesterone concentration was lowest on this phase. During the pregnancy, the $20{\alpha}$-HSD activities were relatively higher early pregnancy(day-1-3 gestation) and late pregnancy(day 21 to parturition), serum progesterone concentration was maintained significantly high to day 19 of gestation. The $20{\alpha}$-HSD activities were lower during the middle pregnancy. From these results, ovarian $20{\alpha}$-HSD activities may possibly act as physiologically very important in the control and maintenance of estrous cycles in rat.

• 대한수의학회지
• /
• 제34권1호
• /
• pp.173-180
• /
• 1994

Milk progesterone concentrations were measured in 111 dairy cows for confirming the estrus observation and for the early pregnancy diagnosis. Of the 56 cows inseminated, 52 cows(92.8%) were an ovulatory estrus, 2 cows(3.6%) were an unovulatory estrus, and 2 cows(3.6%) were the error of estrus observation, respectively. Milk progesterone concentrations at 21 and 24 days after artificial insemination were significantly higher in 23 pregnant cows compared with those in 5 non-pregnant cows(P<0.05). The accuracy rate for early pregnancy diagnosis in 27 cows achieved when the discriminatory concentration at 21 days after artificial insemination was placed at 2.0 ng/ml skim milk, was 91.3% for positive diagnosis and 100% for negative diagnosis, respectively. These results indicated that milk progesterone determination at 0, 6 and 21 days after artificial insemination can be utilized for confirming the estrus observation and for early pregnancy diagnosis. In conclusion, milk progesterone determination is useful diagnostic tool for monitoring the reproductive performance.

• Clinical and Experimental Reproductive Medicine
• /
• 제1권1호
• /
• pp.49-54
• /
• 1974

In the present studies, effect of progesterone on the germinal vesicle break-down of the mouse oocytes cultured in the micro tube was investigated. The results obtained are as follows: As dose of progesterone in the medium rose, accordingly the break-down of the germinal vesicle was suppressed. It was found that $ED_{50}$ was 15.7 ${\mu}g$/ml, and $ED_{90}$ 60.7 ${\mu}g$/ml of progesterone. The dose suppressing the oocyte maturation was apparently higher than that on the rabbit or on the mouse embryonal development. The inhibiting effect of progesterone on the GVBD was reversible. The germinal vesicle of the oocytes were broken down immediately in the medium upon removal of the hormone. Progesterone stops meiosis at any stage upon administration, while dbe AMP or theophylline supresses only the break-down of the nuclear membrane. Recovering of the meiotic division of the oocytes once exposed to progesterone was delayed a little. The inhibiting action of progesterone was not altered by adding more pyruvate or in the presence of higher concentration of the mineral ions in the culture medium.

• 대한수의학회지
• /
• 제33권4호
• /
• pp.611-615
• /
• 1993

젖소에서 progesterone과 testosterone의 측정을 위하여 solid phase 효소면역분석법(酵素免疫分析法)를 개발하였다. 1차 항체로서 $11{\alpha}$-hemisuccinate-progesterone bovine serum albumin과 4-androsten-$17\;{\beta}$-ol-3-one-carboxymethyloxime bovine serum albumin에 대한 토끼 혈청(血淸)을 각각 항혈청(抗血淸)으로 사용하였고, 2차 항체로서 면약 IgG를 사용하였다. Conjugates로서는 각각 progesterone-$11\;{\alpha}$-hydroxy-hemisuccinate-horseradish peroxidase와 4-androsten-$17\;{\beta}$-ol-3-hemisuccinate-horseradish peroxidase를 사용하였다. Progesterone과 testosterone에 대한 최저측정치(最低測定値)는 각각 6.7 pg/well과 1.0 pg/well이었다.

• 한국동물생명공학회지
• /
• 제37권3호
• /
• pp.202-208
• /
• 2022

This study investigated the influence of sodium bicarbonate (NaHCO₃) and progesterone on acrosome reaction and proportion of polyunsaturated fatty acid (PUFA) composition boar sperm. The sperm were diluted with semen extender and incubated with NaHCO₃ and progesterone at 38℃, 5% CO₂ for 6 h. Plasma membrane integrity and acrosome reaction were analyzed using SYBR14/propidium iodide (PI) and FITC-PNA/PI doubling staining method, and proportion of PUFA was analyzed using gas chromatography. In results, Plasma membrane integrity was significantly decreased in 50 mM NaHCO₃ group and acrosome reaction was significantly increased by over the 100 mM NaHCO₃ group compared to control group (p < 0.05). In addition, progesterone significantly increased decreased plasma membrane integrity at 100 mM progesterone and acrosome reaction at over the 5.0 µM progesterone (p < 0.05), but there was no difference among the 5.0 to 100 µM groups. PUFAs were significantly decreased in 100 mM NaHCO₃ and 50 µM progesterone treatments compared to control group. In summary NaHCO₃ and progesterone induce acrosome reaction and reduce PUFA composition in boar sperm, therefore, the results maybe help to understand basically knowledge for the acrosome reaction and PUFA composition in boar sperm.

• 대한수의학회지
• /
• 제35권4호
• /
• pp.833-841
• /
• 1995

젖소 16두를 대상으로 발정주기중 황체의 크기와 progesterone 농도 사이의 상관관계를 알아보기 위하여 황체의 크기를 초음파 진단장치로 측정하였다. 검사우 16두중 9두는 낭종양 황체(corpus luteum with cavity) 나머지 7두는 정상 황체 (corpus luteum without cavity)를 가지고 있었다. Progesterone 농도측정을 위한 우유의 채취 및 초음파 검사는 배란일 부터 배란후 12일 까지는 매 2일 간격으로 그리고 14일 이후부터 다음 배란일 까지는 매일 실시하였다. 발정주기중 황체의 크기와 progesterone 농도는 낭종양 황체 및 정상 황체를 가지고 있는 소들 사이에서 유의성 있는 차이가 없었다. 황체의 크기와 progesterone 농도 사이의 상관관계는 황체발육기(Days 2~8)때 낭종양 황체를 가지고 있는 소가 0.71(p<0.0001), 정상 황체를 가지고 있는 소는 0.74 (p<0.0001) 이었고, 황체퇴행기 (Days -6~0) 때는 0.73(p<0.0001)과 0.76(p<0.0001) 이었으며 전체적으로는 0.70(p<0.0001) 이었다. 이처럼 발정주기중 황체의 크기와 progesterone 농도 사이에는 밀접한 상관관계가 있어서 초음파 진단장치에 의한 황체크기의 측정은 progesterone 농도를 추측할 수 있는 수단으로 이용될 수 있을 것으로 사료된다.