• Journal of Aquaculture
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• v.2 no.2
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• pp.53-90
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• 1989

Feeding proper level of ration matchable with the appetite of fish will enhance production and also prevent waste of food and its consequence, side effects such as pollution of culture medium. To pursue this goal, elaborate studies on dissolved oxygen concentrations- as the major force in inducing appetite and the growth outcome are necessary. The growth of common carp of 67, 200, 400, 600, and 800 gram size groups was studied at oxygen concentrations ranging from 2.0 to 6 mg/$\iota$ in relation to rations from 1 to as many percent of the initial body weight as could be consumed under constant temperature of $25^{\circ}C$. The results from the experiments are summarized as followings; 1. Appetite: The smaller fish exhibited higher degree of appetite than the bigger ones at the same oxygen concentrations. The bigger the fish the less tolerant it was to the lower oxygen thersholds, and the degree of tolerence decreased as ration level increased. 2. Growth : Growth rate (percent per day) increased - unless consumption was suppressed by low oxygen levels- as the ration was increased to maximum. In case of 67 g fish, it reached the highest point of $5.05\%$ / day at $7\%$ ration under 5.0 mg/$\iota$ of oxygen. In case of 200 g fish, the maximum growth rate of $3.75\%$/day appeared at the maximum ration of $6\%$ under 5.5 mg/$\iota$ of oxygen. In 400 g fish, the highest growth of $3.37\%$/day occurred at the maximum ration of $5\%$ and 6.0 mg/$\iota$ of oxygen. In 600 g fish, the highest growth rate of $2.82\%$ /day was at the maximum ration of $4\%$ under 5.5 mg/$\iota$ oxygen. In case of 800g fish, the highest growth rate of $1.95\%$/day was at maximum tested ration of $3\%$ under 5.0 mg/$\iota$ oxygen. 3. Food Conversion Efficiency: Food conversion efficiency ($\%$ dry feed converted into the fish tissue) first increased as the ration was increased, reached maximum at certain food level, then started decreasing with further increase in the ration. The maximum conversion efficiency stood at higher feeding rate for the smaller fish than the larger ones. In case of 67 g fish, the maximum food conversion efficiency was at $4\%$ ration within 3.0-4.0 mg/$\iota$ oxygen. In 200g fish, the maximum efficiency was at $3\%$ ration within 4.0-4.5 mg/$\iota$ oxygen. In 400g fish, the maximum efficiency was at $2\%$ ration within 4.0 - 4.5 mg/$\iota$ oxygen. In 600 and 800g fish, the maximum conversion efficiency shifted to the lowest ration ($1\%$) and lower oxygen ranges. 4. Behaviour: The fish within uncomfortably low oxygen levels exhibited suppressed appetite and movements and were observed to pass feces quicker and in larger quantity than the ones in normal condition; in untolerably low oxygen the fish were lethargic, vomited, and had their normal skin color changed into pale yellow or grey patches. All these processes contributed to reducing food conversion efficiency. On the other hand, the fish within relatively higher oxygen concentrations exhibited higher degree of movement and their food conversion tended to be depressed when compared with sister groups under corresponding size and ration within relatively low oxyen level. 5. Suitability of Oxygen Ranges to Rations: The oxygen level of 2.0- 2.5 mg/$\iota$ was adequate to sustain appetite at $1\%$ ration in all size groups. As the ration was increased higher oxygen was required to sustain the fish appetite and metabolic activity, particularly in larger fish. In 67g fish, the $2\%$ ration was well supported by 2.0-2.5 mg/$\iota$ range; as the ration increased to $5\%$, higher range of 3.0-4.0 mg/$\iota$ brought better appetite and growth; from 5 till $7\%$ (the last tested ration for 67 g fish) oxygen levels over 4.0 mg/$\iota$ could sustain appetite. In 200 g fish, the 2 and $3\%$ rations brought the best growth and conversion rates at 3.5-4.5 mg/$\iota$ oxygen level; from 3 till $6\%$ (the last tested ration at 200 g fish) oxyge groups over 4.5 mg/$\iota$ were matchable with animal's appetite. In 400, 600, and 800 g fish, all the rations above $2\%$ had to be generally supported with oxygen levels above 4.5 mg/$\iota$.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.175-185
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• 1995

This experiment was carried out to investigate the optimum rates of animal excreta(cattle slurry-N) for the highest dry matter production and improve the nutritive values of reed canarygrass with different cutting frequency. The results are summarized as follows; The highest relative dry matter yields for annual dry matter yield were 42.2% and 45.2% at 3rd cut in 3 and 4 cuttings, and 34.7% at 4th cut in 5 cuttings, respectively. The annual dry matter yields of non slurry-N application plot showed were from 6.8 tons to 8.0 tons/ha in all cutting frequencies. The highest annual dry matter yield obtained was in 3 cuttings. The annual dry matter yield increased with an increase of applied rates of slurry-N in all cutting frequencies. Annual dry matter yield was produced by 3.3 tons/ha at rates of 90 kg N/ha/cut in 3 cuttings, and more 1.7 tonsha and 2.4 tons/ha at rates of 30 kg N/ha/cut in 4 and 5 cuttings than that of non slurry-N application plots. The contents of crude protein were 12.01% and 15.0% at rates of 90 kg N/ha/cut in 3 and 4 cuttings, and 16.59% at rates of 120 kg N/ha/cut in 5 cuttings, respectively. On the contrary, the content of acid detergent fibre(ADF) showed the lowest values at same rates of slurry-N application in each cutting. J. The average content of crude protein was significantly increased with cutting frequencies, and they were 11.43%, 13.53% and 15.53% in 3, 4 and 5 cuttings, respectively. The average contents of acid detergent fibre(ADF) were 40.27%, 40.53% and 37.06% in 3, 4 and 5 cuttings. The values of 5 cuttings were significantly lower than other cutting frequencies, but the values of acid detergent fibre(ADF) was not significantly different between 3 and 4 cuttings. 6. The efficiency of dry matter production to slurry-N application rates showed the highest values of 18.9 kg and 15.7 kg DM/kg N at rates of 30 kg N/ha/cut in 3 and 5 cuttings, and 16.3 kg DM/kg/ N at rates of 90 kg N/ ha/cut in 4 cuttings. The efficiency of total nitrogen yield to slurry-N application rates were the highest values of 0.51 kg and 0.43 kg W k g N at rates of 30 kg N/ha/cut in 4 and 5 cuttings, and 0.52 kg TN/kg N at rates of 90 kg N/ha/cut in 3 cuttings, respectively. 7. The ranges of economic slurry-N rates were estimated as the 107.2-151.0 kglha, 359.1-375.7 kgha and 160.3-236.9 kg/ha in 3, 4 and 5 cuttings, and marginal dry matter yields were 9.6-10.0 tons/ha, 12.4-12.6 tons/ha and 9.0-9.7 tons/ha in 3, 4 and 5 cuttings, respectively. 8. The limiting sluny-N application rates to maintain the highest dry matter yields were estimated to be 420.0 kg/ ha, 440.6 kg/ha and 666.3 kg/ha in 3, 4 and 5 cuttings.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.280-286
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• 2012

Clean energy farming is the agricultural activity to improve an efficiency of agricultural energy use and to replace fossil fuels. This study was carried out to establish the clean energy farming system in the controlled cultivation of vegetable crop (cucumber) adopting the biogas production facility. In order to design the clean energy farming system, mass and energy balance was analyzed between the controlled cultivation system and the biogas production facility. Net yearly heating energy demands ($E_{YHED}$) of forcing and semi-forcing cultivation types were 48,697 and $13.536Mcal\;10^{-1}$ in the controlled cultivation of vegetable cucumber. To cover these $E_{YHED}$, the pig slurry of 511 and $142m^3\;10a^{-1}$ (biogas volume of 9,482 and $2,636Nm^3\;10a^{-1}$, respectively, as 60% methane content) were needed in forcing and semi-forcing cultivation types. The pig slurry of $511m^3\;10a^{-1}$ caused N 1,788, $P_2O_5$ $511kg\;10a^{-1}$ in the forcing cultivation type, and the pig slurry of $142m^3\;10a^{-1}$ caused N 497, $P_2O_5$ $142kg\;10a^{-1}$ in the semi-forcing cultivation type. The daily heating energy demand ($E_{i,DHED}$) by the time scale analysis showed the minimum $E_{i,DHED}$ of $7.7Mcal\;10a^{-1}\;day^{-1}$, the maximum $E_{i,DHED}$ of $515.1Mcal\;10a^{-1}\;day^{-1}$, and the mean $E_{i,DHED}$ of 310.2 in the forcing cultivation type. And the minimum $E_{i,DHED}$, the maximum $E_{i,DHED}$, and the mean $E_{i,DHED}$ were 5.3, 258.0, and $165.1Mcal\;10a^{-1}\;day^{-1}$ in the semi-forcing cultivation type, respectively. Input scale of biogas production facility designed from the mean $E_{i,DHED}$ were 3.3 and $1.7m^3\;day^{-1}$ in the forcing and the semi-forcing cultivation type. The maximum $E_{i,DHED}$ gave the input scale of 5.4 and $2.7m^3\;day^{-1}$ in the forcing and the semi-forcing cultivation type.

• Journal of Korean Society of Forest Science
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• v.68 no.1
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• pp.60-68
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• 1985

To study the comparison of the aboveground biomass of Robinia pseudoacacia L. of 8-, 13- and 20-year-old plantations, the experimental plot of $100m^2$ in size located in Youngin-gun were selected. Seven sample trees at different stand ages selected taking account of DBH distribution were felled and the diagram of oven-dry weight distribution of stem, branch and leaf for each 1m segment was constructed. The dry weight of each part of plots was estimated by the method of basal area ratio. If the estimations are extended to a hectare area stand, it contains 36.72, 69.28 and 118.67 tons of the aboveground standing crops in 8-, 13- and 20- year-old stand respectively. The net production was estimated to be as much as 12.56, 13.23 and 16.78 tons per hectare per year and the net assimilation ratio 3.66, 4.13 and 2.50 kg/kg/yr in 8-, 13- and 20-year-old stand respectively. And the efficiency of leaves to produce stem was 1.69, 2.10 and 0.96 kg/kg/yr in same order.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.3
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• pp.205-216
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• 1998

In order to investigate the short-term effect of cattle slurry N application on dry matter yield of reed canarygrass(Phalaris arundinacea L.) in uncultivated rice paddy during the harvested years in 1993~1995. Annual cattle slurry N was applied at the levels of 0, 90, 180, 270 and 360 kg $ha^{-1}$ in 3 cuttings, 0, 120, 240, 360 and 480 kg $ha^{-1}$ in 4 cuttings, and 0, 150, 300, 450 and 600 kg $ha^{-1}$ in 5 cutting, respectively. The results were summarized as follows; 1. The dry matter yields of all cutting frequencies in 1993 were higher than in other harvested years. 2. Significantly higher dry matter yields were obtained as 13.10 tons $ha^{-1}$ at level of 360 kg $ha^{-1}\;yr^{-1}$ in 3 cuttings, 12.50-12.83 tons $ha^{-1}$ at levels of 360-480kg $ha^{-1}\;yr^{-1}$ in 4 cuttings, and 12.52-12.56 tons $ha^{-1}$at levels of 450-600 kg $ha^{-1}$ $yr^{-1}$in 5 cuttings, respectively. 3. Efficiency of dry matter production(kg DM $kg^{-1}$ N) to cattle slurry N application tended to decrease with higher levels of cattle slurry N application and cutting frequencies. Higher efficiencies of dry matter production to cattle slurry N application were obtained as 15.2 kg at N level of 90 kg $ha^{-1}\;yr^{-1}$in 3 cuttings, 19.8 kg at level of 120 kg $ha^{-1}\;yr^{-1}$ in 4 cuttings, and 10.0 kg at level of 150 kg $ha^{-1}\;yr^{-1}$ in 5 cuttings, respectively. 4. The highest relative yields(RY) in different cutting frequency were 38.1% for 2nd cut in 3 cuttings, 41.8% for 3rd cut in 4 cuttings, and 30.1% for 4th cut in 5 cuttings, respectively. 5. The estimated ranges of marginal dry matter yield(Ymar.) were 11.5~12.1 tons $ha^{-1}$ at the ranges of economic N level of 261.0~304.6 kg $ha^{-1}\;$yr^{-1}$ in 3 cuttings, 10.4~11.5 tons $ha^{-1}$ at the ranges of economic N level of 205~302.9 kg $ha^{-1}\;yr^{-1}$ in 4 cuttings, and 11.1~11.6 tons $ha^{-1}$ at the ranges of economic N level of 303.8~354.4 kg $ha^{-1}$ 5 cuttings, respectively. 6. Ranges of economic slurry N level(Necon.) in all cuts were 67.9~95.0 kg $ha^{-1}$ in 3 cuttings, 14.3~119.8 kg $ha^{-1}$in 4 cuttings, and 50.9-125.2 kg $ha^{-1}$ in 5 cutting, respectively. 7. The 3 cuttings per annum was a proper cutting frequency for the increase in dry matter production of reed canarygrass using uncultivated rice paddy.

• Research in Plant Disease
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• v.19 no.4
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• pp.254-258
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• 2013

Fusaric acid (FA) is a mycotoxin produced by Fusarium species. Its toxicity is relatively low but often associated with other mycotoxins, thus enhancing total toxicity. To date, biosynthetic genes or enzymes for FA have not been identified in F. oxysporum. In order to explore the genetic element(s) for FA biosynthesis, restriction enzyme mediated integration (REMI) procedure as an insertional mutagenesis was employed using FA producing-F. oxysporum strains. Genetic transformation of two F. oxysporum strains by REMI yielded more than 7,100 transformants with efficiency of average 3.2 transformants/${\mu}g$ DNA. To develop a screening system using phytotoxicity of FA, eleven various grains and vegetable seeds were tested for germination in cultures containing FA: Kimchi cabbage seed was selected as the most sensitive host. Screening for FA non-producer of F. oxysporum was done by growing each fungal REMI transformant in Czapek-Dox broth for 3 weeks at $25^{\circ}C$ then observing if the Kimchi cabbage seeds germinated in the culture filtrate. Of more than 5,000 REMI transformants screened, fifty-three made the seeds germinated, indicating that they produced little or fewer FA. Among them, twenty-six were analyzed for FA production by HPLC and two turned out to produce less than 1% of FA produced by a wild type strain. Sequencing of genomic DNA regions (252 bp) flanking the vector insertion site revealed an uncharacterized genomic region homologous (93%) to the F. fujikuroi genome. Further study is necessary to determine if the vector insertion sites in FA-deficient mutants are associated with FA production.

• Journal of Korean Society of Forest Science
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• v.104 no.3
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• pp.434-442
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• 2015

The process of cultivation and production of oak mushroom (Lentinula edodes (Berk.) Pegler) on sawdust surface beds were investigated. Sawdust surface bed cultivation is the method by which oak mushrooms are cultured and produced on sterilized sawdust surface bed without using bags. The bed was made by inoculating with 3 to 1 ratio of bed sawdust to oak mushroom mycelial inoculum. The sawdust bed medium with 65% water content was pasteurized at $65^{\circ}C$, inoculated with sawdust spawn and spread on the surface on vinyl film in cultivation shed. During 78 days of cultivation period, water content in the medium varied from 61 to 72%, its pH decreased from 5.6 to 3.9~4.6 and ergosterol concentration increased to $0.33{\sim}0.59{\mu}g/g$. $CO_2$ concentration in the medium rapidly increased to 8.06% in two weeks. In seven weeks the medium surface started browning and $CO_2$ concentration increased to about 5.63%. Until 11th week the $CO_2$ concentration was maintained at 6~7%. After removing the plastic cover on the bed for ventilation in 12 weeks, $CO_2$ within the bed reduced dramatically to 1.5%. In the cultivation shed the internal temperature was $7.1{\sim}29^{\circ}C$ and humidity was 27.3 to 100%, while bed temperature ranged $11.6{\sim}30^{\circ}C$. Oak mushroom fruiting started from late July, in 120 days after bed establishment in late March and continued for approximately 100 days until early December with eight cycles of irrigation treatment. The mushroom yield of the eight cycles were 288~352 kg during the 1st (7/29~8/4) to 3rd cycle (9/3~9/7), 800 kg at the 4th cycle (9/19~9/24), 1,296~1,853 kg during 5th (10/3~10/8) to 7th cycle (4.11~11/9) and 990 kg at 8th cycle (11/23~12/7). Total production was approximately 7.4 tons from 33.0 tons of oak sawdust medium, thus harvest efficiency of the mushroom production was approximately 22.4%.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.5
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• pp.689-700
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• 1996

Fisheries products have to be produced and maintained by work processes from the environment, sometimes helped by people. In Korean fisheries both environmental production and its economic use are included within the windows of system approach. EMERGY is the sum of all inputs expressed as one form of solar energy required directly and indirectly to make a product. Calculating EMERGY flows into Korean fisheries evaluates the real wealth contributed by environmental production and its economic use. Several indices calculated from EMERGY analysis table and a three-arm diagram give perspective on the type and efficiency of the environmental uses. Net EMERGY yield ratio is a measure of its net contribution to the economy beyond its own operation. For adjacent waters fisheries in Korea, the net contribution to the economy is 11.85 or higher, which is a stimulus to the economy that is able to purchase it. EMERGY investment ratio measures the intensity of the economic development and the loading of the environment. The ratio for Korean fisheries as a whole is 0.50, for the adjacent waters fisheries 0.09 and for the shallow-sea cultures 1.28, which is lower than the same index for the industry of the developed country (7.0). The component of environment drawn into production are large compared to purchased investment in Korean fisheries. Much more EMERGY is contained in fisheries products than in the paid services used to process the products. The EMERGY exchange ratio for Korean fisheries as a whole is 6.98, for the adjacent waters fisheries is 10.69 and for the shallow-sea cultures is 1.25. Using market values to evaluate wealth of environment resources is found to be many times too small. Money is paid only to people for their contribution, and never to the environment for its contribution. Macroeconomic value is the appropriate measure for discussing large-scale considerations of an economy, including environment and human goods & services.

• KSBB Journal
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• v.22 no.3
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• pp.162-167
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• 2007

Ammonia gas is one of the major pollutants which cause environmental pollution and damage to the human and the livestock. The objective of this study was to investigate the important parameters for the development of efficient removal of ammonia gas by Bacillius subtilis IB101 and to optimize the medium composition for the mass production of B. subtilis IB101. The ammonia gas removal efficiency was evaluated at different growth phases and by changing culture conditions (temperature, pH). The effect of $(NH_4)_2SO_4$ concentration in preculture medium was examined. Medium optimization for the mass production of B. subtilis IB101 was performed by using Plackett-Burman design and one factor at a time method. The removal of ammonia gas was more efficient at exponential phase by 20% than at stationary phase. The ammonia gas removal was the highest at pH 4 and 30 $^{\circ}C$. There was not any significant influence of concentration of $(NH_4)_2SO_4$ on the removal of ammonia gas. The components of optimized medium for the production of viable Bacillus subtilis IB101 was yeast extract 10 g/l, soluble starch 2.5 g/l, $MgSO_4$ 6 g/l, $CaCl_2$ 1.55 g/l, $(NH_4)_2SO_4$ 5 g/l, $KH_2PO_4$ 0.75 g/l, soy bean meal 8 g/l.

• Journal of Life Science
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• v.24 no.4
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• pp.352-360
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• 2014

We evaluated the growth performance, biochemical characteristics, and immune responses in weaning pigs given a diet containing MR-1 (0.2%/feed) or antibiotics (0.1%/feed) for 45 days. In vitro study showed that MR-1 has antibacterial activity against a variety of strains of pathogenic bacteria, especially a strain of cattle-derived Escherichia coli K99 (E. coli K99) by agar diffusion assay. In the in vivo model, 0.2% MR-1-given group clearly ameliorated the weight gain and feed efficiency in the growth performance of weaning pigs compared to the basal diet group (p<0.05). Additionally, 0.2% MR-1 induced an elevation in the levels of mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) and showed a similar pattern ($TNF{\alpha}$ and $IFN{\gamma}$ production) to the antibiotic treated pigs. Taken together, we suggest that 0.2% MR-1 makes probiotics an alternative to antibiotics in weaning pigs.