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2, 4-Thiazolidindion Induced Plasticity of Myoblast (C2C12) and Satellite Cells (Porcine) - A Comparative Study

  • Singh, N.K.;Chae, H.S.;Hwang, I.H.;Yoo, Y.M.;Ahn, C.N.;Lee, H.J.;Park, H.J.;Chung, H.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.7
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    • pp.1115-1119
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    • 2007
  • This study was conducted to determine the difference between satellite cells (porcine) and myoblasts (C2C12) in their differentiation under the influence of 2, 4-thiazolidindion. C2C12 myoblast cells and porcine satellite cells (isolated from 10 d old $Landrace{\times}Duroc$ piglets) were grown to absolute confluency. Post confluent cells (day 0) were further exposed to adipogenic induction medium along with 2, 4-thiazolidindion ($8{\mu}M$) for 2 d. Thereafter, cells were exposed to 2, 4-thiazolidindion alone every 2 d till day 10 and analysed. The control was cultured in differentiation medium without any treatment. Increased (p<0.05) expression of transcriptional factors i.e. C/EBP-${\alpha}$ and PPAR-${\gamma}$ and transition of cells to adipocyte morphology was noticed from 2 d and 4 d onwards in satellite cells (Porcine) and myoblasts (C2C12) respectively. Myogenesis was observed to be suppressed completely in case of satellite cells compared to myoblasts in response to 2, 4-thiazolidindion. Pax-7 (transcriptional factor) appeared as a sole entity to satellite cells only, as it was not identified in case of myoblasts. Although both the cells were converting to adipoblasts, the degree of their conversion was different in response to 2, 4-thiazolidindion. Therefore, the hypothesis that satellite cells contribute various domains to the growing myoblasts appeared obscured and found to be dependent on the proliferative energy/or degree of fusion. However, it revealed satellite cells as currency to myoblasts/muscle.

Optimizing the fermentation condition of low salted squid jeotgal by lactic acid bacteria with enhanced antioxidant activity

  • Akther, Fahima;Le, Bao;Chung, Gyuhwa;Yang, Seung Hwan
    • Journal of Applied Biological Chemistry
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    • v.60 no.4
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    • pp.391-402
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    • 2017
  • Lactic acid bacteria (LAB) are widely used as starter culture in food fermentation due to their harmless entity and health beneficial properties along with the ability to change texture, aroma, flavor and acidity of food products. In this study, five different LAB (FB003, FB058, FB077, FB081, and FB111) isolated from different Korean traditional fermented foods, assigned to Lactobacillus plantarum, Pediococcus pentosaceus, Weissella viridescens, Lactobacillus sakei, and Leuconostoc mesenteroides, respectively, on the basis of their physiological properties and 16S rRNA sequence analysis, to use as fermentation starter and check their ability to fasten the ripening time as well as the overall optimization in the fermentation condition. To check their suitability as starters, their safety, acid and bile tolerance, NaCl and temperature resistance, susceptibility to common antibiotics, and antimicrobial activities were determined. Squid jeotgal samples were prepared by adding $10^8CFU/g$ of each strain in different samples, which were then kept for fermentation at $4^{\circ}C$ and checked for their antioxidant activities at 0, 7, 15, and 21-day intervals. The samples fermented with FB003 and FB077 displayed the highest antioxidant activity. This study revealed two effective starter cultures (FB003, FB077) for squid jeotgal fermentation, which presented increased functionalities. The results of this study will lead to the development of novel industrial-scale production avenues for jeotgal preparation, and offer new insights into the prevention and control of chronic diseases.

Investigation of bycatch and discards of funnel net in the coastal waters of Yeosu (여수 연안 소형 정치망어업에 있어서 혼획과 투기 조사)

  • Shin, Hyeong-Ho;Jeong, Sun-Beom;Oh, Taeg-Yun;Shin, Jong-Keun;Cho, Young-Bok;Kim, Hyung-Seok
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.46 no.4
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    • pp.324-334
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    • 2010
  • This investigation was carried out to research on the actual states for bycatch and discards of catches caught by funnel net from December, 2007 to June, 2010. Fishing grounds were Manheung-dong and Ocheon-dong in the coastal waters of Yeosu. The number of the investigation was 21 in Manheung-dong and 7 in Ocheon-dong fishing ground. In Manheung-dong, the species of catches were 26 of fish, 6 of cephalopod and 5 of crustacean. In Ocheon-dong, the species of catches were 25 of fish, 6 of cephalopod and 4 of crustacean. In the two all fishing grounds, the largest amount of species was revealed as gray mullet. Among these catches, the number of non marketable species that were classified and discarded ones, were 9 in Manheung-dong and 7 in Ocheon-dong. Also, among the marketable species which were small entity or too tiny catches to commercialize and not fresh ones were discarded. The proportion that they dominate in the whole catches was 37.1% in the number of catches and 5.4% in the weight of catches in Manheung-dong, and 6.9% in the number of catches and 0.3% in the weight of catches in Ocheon-dong. In case of Manheung-dong, the monthly discarding proportion was the highest with 59.4% in the number of catches in may and 17.6% in the weight of catches in November, and in case of Ocheon-dong, the both of them were the highest in February, with 28.0% in the number of catches and 5.1% in the weight of catches.

A Case of Antiphospholipid Syndrome Associated with Autoimmune Thrombocytopenic Purpura (자가면역 혈소판감소성 자반병과 관련된 항인지질 증후군 1례)

  • Nam, Y.S.;Lee, W.S.;Park, C.;Yoon, T.K.;Cha, K.Y.
    • Clinical and Experimental Reproductive Medicine
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    • v.26 no.2
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    • pp.265-269
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    • 1999
  • Thrombocytopenic patients without detectable bound antiplatelet antibody should be diagnosed with idiopathic thrombocytopenic purpura (ITP) if no other cause of their decreased platelet count could be found. More recently the term "autoimmune thrombocytopenic purpura (ATP) has supplanted ITP since the disease is related to the production of autoantibodies against one's own platelets. This entity should not be confused with isoimmune thrombocytopenic purpura (also called alloimmune thrombocytopenic purpura). In this cases maternal antiplatelet antibodies directed against the PLA 1 antigen on the fetal platelets causes severe fetal and neonatal thrombocytopenia in a situation analogous to Rheusus disease. Antibodies to the negatively charged phospholipids, lupus anticoagulant, and anticardiolipin have been linked to adverse pregnancy events. Pregnant women possessing these antibodies have an increased risk of spontaneous abortion, stillbirths, intrauterine fetal growth retardation, preterm birth, and arterial and venous thrombosis. Antiphospholipid antibodies decrease or may even disappear between pregnancies only to recur with increased activity in a subsequent pregnancy and lead to loss. We have experienced a case of antiphospholipid syndrome associated with autoimmune thrombocytopenic purpura in patient with recurrent spontaneous abortion. So we report this case with a brief review of literatures.

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Developments in Space Law in regulating commercial space activities (우주(宇宙) 상업활동(商業活動) 규율(規律)에 있어서의 우주법(宇宙法)의 발전적(發展的) 위상(位相))

  • Shin, Hong-Kyun
    • The Korean Journal of Air & Space Law and Policy
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    • v.3
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    • pp.233-258
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    • 1991
  • This paper examines current developing status of space law as defined in terms of its unique principle that "the utilization of outer space for all mankind's interest". As commercialization of space activities has brought about heated debate on its legitimacy with respect to that principle, space law has come to witness a dual situation. One is the realization of that principle in establishing commercial space activities system where all mankind's interest is respected through the non-discriminatory distribution mode of space benefit. In satellite telecommunication system, the INTELSAT Organization assures its public service policy while protecting itself against a competition from separate systems. For remote sensing, commercialization of LANDSAT Systems promoted since 1984 seems not to affect present non-discriminatory distribution of data and information obtained. On the other hand, active participation of private entity aiming at commercial profit enables national government to manage more effective control and supervision of those activities with a view to promoting national interests. Also, newly developed private rules and regulations are emerging in business relations about commercial space activities. Extended capacity of national government assuring its national interest in production and distribution of international resources that is outer space, and regulation of space activities by newly developed rules and customs provoke a concern about a identity problem of space law. The best way to summarize this perspective might consist in saying that non space law regulations, which are not assumed as aiming at the promotion of all mankind's interest, are confirming their role about space related activities. For those reasous, we are now facing a developing status of space law as developments of commercial space activities continues.

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A Study on Conservation of the Natural Environment in Chosun-Dynasty, Korea (조선시대 자연환경보전에 관한 연구)

  • 오승봉;안동만
    • Journal of the Korean Institute of Landscape Architecture
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    • v.23 no.1
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    • pp.51-65
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    • 1995
  • Conservation of the natural environiment In chosun-Dynasty, Korea, is analysed on the basis of the of official records of the king's offices(Chosun-wangjo-sillok). In the popular ideologies of the dynasty, Seongleehak(philosophy of humanity arid natural laws) and Pungsu (geomancy) , the naturnal enviroment is defined as an entity con-sisting of Cheon (heaven) , San(mnountains) , Su (water), Geumsu (animals)and Chomok (plants) .the notion of Tacksi-Tackmul(being careful to take natural resources at the right time) was one of the Cheonmyung (eavenly decrees) . It was believed that violation of this principle resulted in natural disasters. Sasan(four mountains surrounding Hansungbu-, the capital city, now Seoul) , were strictly preserved according to geomancy practices. In areas other than the capital city, Sanlimcheontack (mountains, forests, rivers and ponds) were con-served for sustained production of building materials especially pinetrees for ship building, foods, firewoods, horses, and orter useful natural resources. Various conservation policy insturuments were adopted. Prunning trees was permitted only in October. Capturing fishes was allowed only after the spawning period. Protection areas were designated in Sasan, Seongjoesiplee (surroundings of the capital city) , Geumsan(protected mountains), Kangmujang(hunting and army training grounds), Sijang(firewood areas), Mokmajang(horse ranches) and Neungyuk(royal tombs) . Activities prohibited for conservation purposes included cutting timbers, burning, building houses or tombs, dumping wastes, farming and breaking up fresh land, grazing, hunting and trespassing. Positive actions for conservation were rituals to Heaven ennoblement of natural elements such as mountians or rivers, planting trees, Boto (supplementing soil on low ridges) , Josan (mounding) and making ponds. Boto, Josan and making ponds were to make ideal terrains for geomancy. Many government bodies and civil servants were engaged in the conservation activities. For example, Sanjik(forest keeper) was a special position with responsibility for forest management.

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Grazing Behavior and Forage Selection of Goats (Capra hircus)

  • Lee, Sang-Hoon;Lee, Jinwook;Chowdhury, M.M.R.;Jeon, Dayeon;Lee, Sung-Soo;Kim, Seungchang;Kim, Do Hyung;Kim, Kwan-Woo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.3
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    • pp.189-194
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    • 2019
  • The normal feeding approach of goats might be due to their precise anatomical and physiological characteristics of entity, which permit them to be highly selective, to eat legume silages and wild green grass. This review has been designed to consider the grazing behavior, fodder selection, and feed composition of goats. Various herbs and corns consumed by goats have numerous nutritive resources. Based on the general herbaceous intake activities and behavior of goats, they prefer wild grass such as grass grown in the steep hills than soft grass. Because the digestion capacity of cellulose feed has higher digestion level compared to other non-ruminants within rumen and it is advantageous to use wild forest or mountain grass which comprises high proportion of cellulose feed for goat. In South Korea, there are abundant feed resources for goats because of occupying large areas of mountains. Thus, goat production and feeding costs could be reduced if plants are used from the wild forest as a feed for goats relative to grassland grazing. Also, it is expected to contribute in improvement of goat farming with harmonious relationship between the grassland and wild forest while satisfying animal welfare and physiological desires of livestock.

Anti-inflammatory Effects of Canavaliae Semen(Canavalia gladiate) Extracts in a Systemic Anaphylaxis Food Allergy Mouse Model (전신과민성 식품 알레르기 마우스 모델에서의 도두(刀豆) 추출물의 항염 효과)

  • Yang, Won-Kyung;Park, Yang-Chun;Kim, Han-Young;Kim, Geun-Hui;Noh, Seong-Soo;Kim, Seung-Hyung
    • The Korea Journal of Herbology
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    • v.34 no.1
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    • pp.1-11
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    • 2019
  • Objective : An allergy to peanuts is a major cause of fatal food-induced anaphylaxis, with food allergies becoming an increasingly important health research issue. Food allergy as clinical entity has been recongnized for many years, although there is yet no general concord as to the incidence of this symptom.1) Methods : This study was undertaken to verify the effect of seeds of Canavalia gladiata (Jacq.) DC. extract (CGE) on the inhibition of allergic reactions using a cholera toxin and peanut extract-immunized food allergy mouse model. We determine whether the changes in rectal temperature were related to energy consumption owing to heat production in the body. Mast cell distribution and degranulation in the dermis and epidermis were observed with an optical microscope. Subsequently, Ara h1 levels in serum and interleukin (IL)-4, IL-10, and $IFN-{\gamma}$ levels in cultured supernatants of splenocytes were measured. Results : CGE treatment significantly attenuated the secretion of the Ara h1 antibody in serum and splenocytes. Ara h 1 was undetected in the cholera toxin and peanut extract-immunized food allergy mouse model. Improvement in ear tissue inflammation symptoms was the CGE experimental group. In the control group and peanut extract control group, the expression of mast cells was higher, whereas that in the CGE experimental group was significantly lower. Conclusion : CGE causes suppression in a food allergy mouse model via the inhibition of Ara h1 secretion, and might be useful for developing functional health foods.

A Study on the Architectural Design Utility of Object-based CAD System (객체기반 CAD 시스템의 건축설계적 효용에 관한 연구)

  • Yoo, Chang-Geun
    • Journal of The Korean Digital Architecture Interior Association
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    • v.2 no.1
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    • pp.9-15
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    • 2002
  • As architectural forms tend to be large-scaled, high-storied and complicated, use of computer for processing design information has been generalized. However, CAD use in the process of developing architectural design has been neglected greatly in the educational field of architectural field due to the surging of recognition that it may limit the essential property of architectural design. It may be resulted from the limitation of CAD system, that is, it is because of tardy speed of application development for the related areas with the lack of simplicity and clarity of flexibility to be secured on the drawing and user interface. Recent CAD systems, however, overcome such a limitation, convert into object-based design from entity-based drawing, drafting and modeling for implementing design concept of architects and it connects with internet linked to superspeed information communication network and changes process and stream of architectural design. Therefore, this study deals with utility of object based CAD system with products of Autodesk Co. and consequently obtain the following conclusions. First, it expands architect's design areas by supporting cooperative design system based on model-based architectural design and internet. Second, it reduces consumption of personal and material resources and time in the process of drawing production for improving architectural design works. Third, it can reduce the frequent design changes by improving understanding of architectural space with visualization of immediate 3D information, escaping from traditional methods to deliver design information to building owner through 2D drawing or model and perspective drawing requiring much cost and time. Fourth, it keeps exactness without omission or duplication of design information and generate and renew information of all related drawings on a drawing. Fifth, it is possible to change difficult and boring architectural design work into a pleasure owing to immediate modeling and drawing of design idea. In addition, digital drawing generated by using object-based CAD system can playa role of establishing Urban Information System to be used for protecting from building in disaster and urban disasters in connection with GIS numerical map and be used for reference of all kinds of information required fro post-management of buildings. However, what is more important is that surplus time from introduction of object-based CAD system should be used for consideration to be recognized as a good space and building built as a product of this project for comfort to urban people.

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Augmentation and Monitoring of an Endangered Fish, Gobiobotia naktongensis in Naeseongcheon Stream, Korea (내성천에서 멸종위기어류 흰수마자 Gobiobotia naktongensis의 증강도입과 모니터링)

  • Na, Jin-Young;Choi, Byoung-Seub;Hwang, Sang-Chul;Yang, Hyun
    • Ecology and Resilient Infrastructure
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    • v.2 no.3
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    • pp.216-223
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    • 2015
  • The conservation project to protect an endangered fish, Gobiobotia naktongensis was executed against declining the gene diversity of the fish after the construction of Youngju Dam in a sand-bed stream (Naeseongcheon Stream). We tried to move the populations of G. naktongensis from submerged planned sites to alternative habitats, bred artificially and augmented the juveniles to optimal habitat, and monitored the results of the restoration implementation. No entity of G. naktongensis was confirmed at the planned submerged sites despite attempting to capture more than 8 times and eventually the movement to alternative habitat could not be implemented. About 40 individuals of G. naktongensis were captured in the Naeseongcheon Stream and a total of 5,000 individuals were artificially spawned up. The population of juvenile inherited the genetic diversity from the brood stock. The bred juveniles were discharged at the selected optimal site that had a habit condition what was similar to their natural habitat. The micro-dispersion around the discharging area was found at the early stages of the augmentation. The re-capturing rate of discharged juveniles was reduced as time passed. The discharged juveniles seemed to adapt to the natural environment of Naeseongcheon Stream. The observation of their high abdominal distension and excrement demonstrated that the juveniles fed successfully in the discharging area. Therefore, securement of genetic homogeneity and enhancement of restoration population of G. naktongensis in upstream and downstream of Youngju Dam site from the artificial seed producing seemed to be primarily successful and long-term monitoring and analysis of the effect was expected to be necessary.