• Title/Summary/Keyword: Production Education

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Repair of UV-induced Cyclobutane Pyrimidine Dimers in Human Mitochonrial DNA-less Cells

  • Ikushima, Takaji;Gu, Ning;Tanizaki, Yuichi
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.479-481
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    • 2002
  • UV-induced DNA damage causes cell killing and mutations leading to carcinogenesis. In normal human cells, UV damage such as cyclobutane pyrimidine dimers (CPDs) and primidine-prymidone (6-4) photoproducts are mainly repaired by nucleotide excision repair mechanism. The molecular processes have been well characterized recently. To know the influence of mitochondrial genome on the nucleotide excision repair mechanism against CPDs, we comparatively examined the production of CPDs by UVC irradiation and their repair kinetics in human cells completely lacking mitochondrial DNA (mtDNA) and the parental HeLa S cells. Whole DNA extracted from the cells exposed to UVC was treated with T4-endonuclease V to break the phosphodiester bond adjacent to CPDs. The DNA was electrophoresed in a denaturing agarose gel, which was visualized by ethidium bromide staining. The relative amount of CPDs was determined by image analysis using NIH Image software. MtDNA- less (rho-O) cells were apparently more sensitive to UVC than HeLa S cells, while the level of induction of CPDs in rho-O and HeLa cells was comparable. The repair of CPDs was less efficient in rho-O cells compared with HeLa cells. The residual amount of CPDs after 24-h repair was larger in rho-O cells than in HeLa cells where more than 90 % of CPDs were repaired by then. The non-repaired CPDs would lead to apoptosis in rho-O cells. These results suggest that mitochondrial genome may contribute to some ATP-dependent steps in nucletide excision repair by supplying sufficient ATP which is generated through a respiratory chain in mitochondria.

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A Study on Systematic Model Development of Skill Improvement for Industrial Engineers (PLC Based Control) (기업체 현장엔지니어 향상 프로그램의 체계적인 모형개발에 대한 연구(PLC 기반 제어를 중심으로))

  • Kim, Jin-Woo;Lee, Woo-Young
    • The Journal of Korean Institute for Practical Engineering Education
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    • v.1 no.1
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    • pp.19-24
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    • 2009
  • It is necessary that the industry has to develop various automated control technology for efficient creation of manufacture automation system according to rapid market situation. One of the technologies is the fused complex control based on PLC-based controlled system. According to rapid growth and distribution of various automated control technologies using PLC, the engineers in automation, Production and manufacturing technologies fields have difficulties in systematic studying on the technologies by choosing an optimal route due to various industry-applied examples and ranges, in spite that the technology is essential. Therefore, the researchers indicate applied outputs and effects extracted by systematically developing systematic company-specified training program by analyzing education procedure drawbacks for S-company engineers.

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Utilization of Fruit Processing Wastes in the Diet of Labeo rohita Fingerling

  • Deka, Abani;Sahu, N.P.;Jain, K.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.11
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    • pp.1661-1665
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    • 2003
  • A feeding trial was conducted for 60 days to study the utilization of fruits processing wastes as feed ingredient in the diet of Labeo rohita fingerlings. One hundred and sixty fingerlings (av. body weight, $1.65g{\pm}0.03$) were equally distributed in four experimental groups having 4 replicates each. Four different experimental diets were prepared by replacing wheat flour and rice bran with either orange (T2) (Cirtus qurantium), pineapple (T3) (Ananas spp. and Pseudananas spp.) or sweet lime (T4) (Citrus sinensis) wastes to the basal diet along with the control (T1, without any fruit wastes) keeping the CP level at around 40%. The water quality parameters like DO, $CO_2$, pH, total alkalinity, total hardness, ammonia and water temperature were recorded within the optimum range. The diet containing 25% pineapple wastes (T3) showed significantly higher growth in terms of SGR (1.50), FCR (2.09) and PER (1.19) than the other groups. However, growth of T4 and T2 groups were not significantly different than the control group (T1). Protease activity (17.17 unit/mg protein), protein digestibility (91.57%) and carbohydrate digestibility (41.62%) were not significantly different among the different groups. Survival of the fingerlings were not significantly different among the experimental groups. It concludes that waste of orange, pineapple and sweet lime can be used at 25% level as a substitute of wheat flour and rice bran in the diet of Labeo rohita.

Short-term grazing behavior of cattle under indoor housing for a new-bred tetraploid ruzigrass (Brachiaria ruziziensis Germain et Everard)

  • Ishigaki, Genki;Nitthaisong, Pattama;Prasojo, Yogi Sidik;Kobayashi, Ikuo;Fukuyama, Kiichi;Rahman, Mohammad Mijanur;Akashi, Ryo
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.5
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    • pp.748-754
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    • 2018
  • Objective: The preference evaluation of cattle is an important factor for estimation and improvement of the grazing amounts of newly introduced or bred grasses or cultivars in barn. This study was performed to assess the grazing behavior (the amount of grazing and/or the grazing speed) of cattle as indirect method using newly bred Brachiaria ruziziensis tetraploid strain 'OKI-1'(BR) hay as treatment group and Cloris gayana 'Callide' (CG) hay as control group. It also compared the feasibility of using behavioral differences between two groups as one criteria for evaluating preference by Japanese black cattle in barn. Methods: Three experiments were carried out using 12 growing Japanese Black cattle including 6 males and 6 females. In each experiment, the four Japanese Black cattle (2 males and 2 females) were placed in separated stall and allowed to graze BR and CG in manger that was separated into two portions for about 30 min. The position and behavior of the cattle were recorded, and weighed the residual of each gay at 15 and 30 minutes after experiment start. Results: The BR was superior to CG in chemical composition such as protein, fibers and non-fibrous carbohydrate. The cattle, over all, tended to prefer BR over CG in the first half 15 minutes in terms of the time spent and amount of grazing. Additionally, growing cattle exhibited neophilia for BR bred newly. Conclusion: These findings indicated the current approach could be applied for one of criteria to evaluate the preference of hay by Japanese black cattle under indoor housing environment.

Development of Equipment Control System based on DB Access Method for Industrial IoT (Industrial IoT를 위한 데이터베이스 접근 기반 장비 제어 시스템 개발)

  • Cho, Kyoung-woo;Jeon, Min-ho;Oh, Chang-heon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.20 no.6
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    • pp.1142-1147
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    • 2016
  • Recently, IoT(Internet of Things) has been extensively researching to provide intelligent services by fusing ICT. Especially with the advent of Germany's Industry 4.0, it is emphasized the importance of the industrial IoT to maximize the production capacity. Accordingly, a lot of efforts to spread the smart factory base of industrial IoT have continued domestically as well as abroad. But the current smart factory systems have controlled equipment using the data declared in the embedded systems. Therefore, it is difficult to control environment that lots of equipment is installed. In this paper, we proposed equipment control system based on data base access method for industrial IoT. This method controls the equipment using data base from parameter of equipment. Through experiments that the system apply to mold shot system with a number of variables, it is shown that the proposed method can efficiently control a number of devices.

Silage preparation and fermentation quality of natural grasses treated with lactic acid bacteria and cellulase in meadow steppe and typical steppe

  • Hou, Meiling;Gentu, Ge;Liu, Tingyu;Jia, Yushan;Cai, Yimin
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.6
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    • pp.788-796
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    • 2017
  • Objective: In order to improve fermentation quality of natural grasses, their silage preparation and fermentation quality in meadow steppe (MS) and typical steppe (TS) were studied. Methods: The small-scale silages and round bale silages of mixed natural grasses in both steppes were prepared using the commercial lactic acid bacteria (LAB) inoculants Chikuso-1 (CH, Lactobacillus plantarum) and cellulase enzyme (AC, Acremonium cellulase) as additives. Results: MS and TS contained 33 and 9 species of natural grasses, respectively. Stipa baicalensis in MS and Stipa grandi in TS were the dominant grasses with the highest dry matter (DM) yield. The crude protein (CP), neutral detergent fiber and water-soluble carbohydrate of the mixed natural grasses in both steppes were 8.02% to 9.03%, 66.75% to 69.47%, and 2.02% to 2.20% on a DM basis, respectively. All silages treated with LAB and cellulase were well preserved with lower pH, butyric acid and ammonia-N content, and higher lactic acid and CP content than those of control in four kinds of silages. Compared with CH- or AC-treated silages, the CH+ AC-treated silages had higher lactic acid content. Conclusion: The results confirmed that combination with LAB and cellulase may result in beneficial effects by improving the natural grass silage fermentation in both grasslands.

A Comparative Study on the Sewing Teaching of Tailored Jacket (테일러드 재킷 봉제방법 비교 연구 - 의복구성 교재를 중심으로 -)

  • Shin, Jang-Hee;Sohn, Hee-Soon
    • Journal of the Korea Fashion and Costume Design Association
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    • v.11 no.2
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    • pp.75-84
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    • 2009
  • The following research analyzed the sewing teaching of the tailored jacket shown in fashion textbooks, which are currently used in universities and educational organizations in the fashion industry. To analyze the sewing teaching of tailored jackets, we analyzed 35 textbooks in the market. Among them we used 14 textbooks which dealt with the patter formation method of tailored jackets, and cutting and sewing methods. First, the stitching direction and the location of attachment varied. The type of sticking tape and its position also differed, emphasizing the need to make an institutionalized education material which is based on the characteristic of the fabric and its silhouette. Second, controlling the ease length of the right side sewing princess line, or information about the shoulder line and sleeve line of the notch as not specifically shown in the textbooks. Furthermore, not many textbooks showed how to pull the lower part of the texture out of sight, nor did they explain why this needed to be done. Third, the lining is usually slightly larger than the outer texture of the clothing, as the lining is usually tugged in the sewing process, and this method makes the clothes more comfortable. The method of sewing the princessline $0.2{\sim}0.3cm$ outside the outer pattern was the most recommended. The center-back side was usually 2cm in activity measurements, but it turned out that a textbook which required ease treatment on the shoulder line did not do so on the lining material. The textbooks used in this research dealt only with how to manufacture a tailored jacket without full explanation about textures which could influence the manufacturing. The production method and process differed between different textbooks, showing the need to make a institutionalized education material. In a quickly-changing era where there is rapid change in the dressmaking procedure, we need to help students understand the process more easily through a formalized education process.

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Production of Chemistry Laboratory Class for Senior High School Freshmen

  • Yasuzawa, Mikito;Minagawa, Keiji;Kamitani, Sachiyo;Arai, Yuka;Konishi, Yuki;Nakanishi, Shinsuke;Oshima, Takuya;Yamaguchi, Junko;Ishii, Arisa
    • Journal of Engineering Education Research
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    • v.13 no.5
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    • pp.55-60
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    • 2010
  • Chemistry laboratory class was produced for senior high school freshmen with the cooperation of university staffs, high school teachers and the university students. Although the students who will take the lab class are senior high school freshmen, we decided to prepare four experiments that are simple and have highly visible reactions or transformation. That is, 1) Water purification, 2) Surface modification, 3) Briggs-Rauscher reaction, and 4) Polymer synthesis and characterization. After the safety guideline and experiment instructions by the faculty staffs, two teaching assistants (TAs) supervised each experiment. Since taking a direct part in it will provide stronger impact than only being one who is just watching the experiment, all experiments contained some process that the high school students must handle the reagents, tools or the equipment, by themselves. Although, the operation performed by the students was limited to a series of simple actions, the various unique phenomena presented by the experiments impressed the students. The lab class was fruitful not only for high school students, but also good for university students. The lab class provided good opportunity for them to improve the abilities to teach and guide someone.

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A Study on Surveillance System in Korea's National and Public Museum - Focussed on Indirect Surveillance System in Conjunction with Spatial Structure of Museums - (국내 국, 공립 뮤지엄 감시체계에 관한 연구 - 공간구조에 의한 간접감시체계를 중심으로 -)

  • Moon, Jung-Mook
    • Korean Institute of Interior Design Journal
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    • v.20 no.2
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    • pp.176-183
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    • 2011
  • Modern museums that started from 17th century in Europe have played a role of storage for collections of the upper class as a form of private museum. Since 18th century the publicity has been more emphasized in the society of Europe and there became a public museum that serves as a institution for social education. In addition to this, the mass production that began with the Industrial Revolution relieved the working hour of the public and they started to spend their more time for visiting museums to have social education. In korea there constructed a lot of public museums after economic development and people experienced the social education and the cultural events. However, there raised questions on security issue regarding relic because of growing number of visitors to the museums. Due to this, the museums asked a surveillance system for their relic. But the museums don't have enough research on this nor understanding the current situation. Because the relic in museums of history has a money value, the anti-theft system is more focussed. In addition to the direct surveillance system with staff or closed-circuit television(CCTV), the indirected system with visitor's mutual surveillance in conjunction with spatial structure has to be researched. This study is to understand the relationship between surveillance system and spatial structure with reference to korea's national and public museum of history. With the results of the research, the study provides a planning guideline to the museum architecture to prevent security problem.

Analysis of learner needs for advanced dental hygiene major courses (치위생과 전공심화과정을 위한 학습자 요구분석 및 결과활용 사례)

  • Jung, Young-Ran;Choi, Hye-Sook
    • Journal of Korean society of Dental Hygiene
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    • v.9 no.1
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    • pp.193-205
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    • 2009
  • The purpose of this study was to examine the needs of learners in an advanced major course in a bid to strike a balance between theory and practice. And it's also meant to suggest an actual case of that effort in an advanced course. As a result of analyzing the collected data, the largest number of the learners who signed up for the advanced course chose that course to earn a bachelor's degree, and the second greatest group did that to bolster their expertise. The name of the college that offered that course (41.4%) had the most impact on their choice of it, followed by accessibility(28.6%). The most common thing they practiced in the oral health education course as one of major courses was forming a lesson plan, followed by teaching at schools and kindergartens, trial student teaching, producing PPT, making OHP materials, making a bulletin board and producing leaflets. During clinical activities for oral health education, the largest group considered it necessary to learn about more theories on educational psychology(54%), followed by the production of teaching media(29.9%) and teaching methods(25.3%). Likewise, the greatest group found it more necessary to practice educational psychology(42.5%), followed by the utilization of media(37.9%) and teaching methods(28.7%). An one-on-one interview was implemented before and after their classes to obtain more detailed information on their needs, and what their needs were in relation to the given curriculum was eventually confirmed, which made it possible to offer better education in response to their needs.

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