• Title/Summary/Keyword: Production Education

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Exoproduction and Biochemical Characterization of a Novel Serine Protease from Ornithinibacillus caprae L9T with Hide-Dehairing Activity

  • Li, Xiaoguang;Zhang, Qian;Gan, Longzhan;Jiang, Guangyang;Tian, Yongqiang;Shi, Bi
    • Journal of Microbiology and Biotechnology
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    • v.32 no.1
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    • pp.99-109
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    • 2022
  • This study is the first report on production and characterization of the enzyme from an Ornithinibacillus species. A 4.2-fold increase in the extracellular protease (called L9T) production from Ornithinibacillus caprae L9T was achieved through the one-factor-at-a-time approach and response surface methodological optimization. L9T protease exhibited a unique protein band with a mass of 25.9 kDa upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This novel protease was active over a range of pH (4-13), temperatures (30-80℃) and salt concentrations (0-220 g/l), with the maximal activity observed at pH 7, 70℃ and 20 g/l NaCl. Proteolytic activity was upgraded in the presence of Ag+, Ca2+ and Sr2+, but was totally suppressed by 5 mM phenylmethylsulfonyl fluoride, which suggests that this enzyme belongs to the serine protease family. L9T protease was resistant to certain common organic solvents and surfactants; particularly, 5 mM Tween 20 and Tween 80 improved the activity by 63 and 15%, respectively. More importantly, L9T protease was found to be effective in dehairing of goatskins, cowhides and rabbit-skins without damaging the collagen fibers. These properties confirm the feasibility of L9T protease in industrial applications, especially in leather processing.

Optimizing Graphene Growth on the Electrolytic Copper Foils by Controlling Surface Condition and Annealing Procedure (전해구리막의 표면 조건과 어닐링 과정을 통한 그래핀 성장 최적화)

  • Woo Jin Lee;Ha Eun Go;Tae Rim Koo;Jae Sung Lee;Joon Woo Lee;Soun Gi Hong;Sang-Ho Kim
    • Journal of the Korean institute of surface engineering
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    • v.56 no.3
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    • pp.192-200
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    • 2023
  • Graphene, a two-dimensional material, has shown great potential in a variety of applications including microelectronics, optoelectronics, and graphene-based batteries due to its excellent electronic conductivity. However, the production of large-area, high-quality graphene remains a challenge. In this study, we investigated graphene growth on electrolytic copper foil using thermochemical vapor deposition (TCVD) to achieve a similar level of quality to the cold-rolled copper substrate at a lower cost. The combined effects of pre-annealing time, graphenized temperature, and partial pressure of hydrogen on graphene coverage and domain size were analyzed and correlated with the roughness and crystallographic texture of the copper substrate. Our results show that controlling the crystallographic texture of copper substrates through annealing is an effective way to improve graphene growth properties, which will potentially lead to more efficient and cost-effective graphene production. At a hydrogen partial pressure that is disadvantageous in graphene growth, electrolytic copper had an average size of 8.039 ㎛2, whereas rolled copper had a size of 19.092 ㎛2, which was a large difference of 42.1% compared to rolled copper. However, at the proper hydrogen partial pressure, electrolytic copper had an average size of 30.279 ㎛2 and rolled copper had a size of 32.378 ㎛2, showing a much smaller difference of 93.5% than before. This observation suggests this potentially leads the way for more efficient and cost-effective graphene production.

The Problems and Improvement of Farmers Educational Programs (농업인 교육 프로그램 문제점 및 개선방안)

  • Yu, Byeong-Min;Kim, Jung-Joo;Choi, Yong-Chang;Park, Hye-Jin;Kim, Sun-Hee
    • Journal of Agricultural Extension & Community Development
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    • v.17 no.1
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    • pp.45-74
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    • 2010
  • The purposes of this study were to analyze the farmer education programs in Korea and to develop the strategies for improving its effectiveness. Questionnaires were used to collect data from institutes of farmer education. The framework of analysis was developed with four survey areas and 328 programs were analyzed based on it. The results show that the major learning domains of farmer education were concentrated on production skills and were overlapped with other training institutes. There was no systemic structure in whole programs for farmers. Evaluations for learning achievement were not fully implemented. It was recommended that there should be a system for improving quality of farmer education.

Reactions of Organosilyl and Organostannyl-Sulfides with Isocyanates (Organosilyl 및 Organostannyl-Sulfide와 Isocyanate의 반응)

  • Song Yoon Hahn;Dong Yul Lee;Il Kyu Lee;Paek U Hyon
    • Journal of the Korean Chemical Society
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    • v.17 no.3
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    • pp.188-192
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    • 1973
  • Reactions of trimethylsilylethylsulfide, trimethylsilylphenylsulfide, triethylstannylethylsulfide, and triethylstannylphenylsulfide with isocyanates were studied at various temperatures for 10 days. These Si-S and Sn-S bond compounds catalyzed the production of the cyclic dimer and trimer of phenylisocyanate, diphenylcarbodiimide and 1,3,5-triphenyl-4-phenyliminohexahydro-1,3,5-triazine-2,6-dione in the reaction of phenylisocyanate. In contrast, these compounds gave only the cyclic trimer, triethylisocyanurate, from ethylisocyanate.

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A Study on IEEE 802.15.4 for wireless Communication of Data in the Factory Automation System (공장자동화시스템에서 데이터 송수신의 무선화를 위한 IEEE 802.15.4에 관한 연구)

  • Lee, Hye-Rim;Moon, Il-Young
    • Journal of Advanced Navigation Technology
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    • v.13 no.2
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    • pp.238-243
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    • 2009
  • Now, the production process systems are largely based on automatic system using the wired network. The production process systems using wired network has disadvantage that it is expensive when the installed and replaced equipment. The each equipment happens to repair cost for control and management in production processes. And the replaced equipment has also the additional expense and breaks production process. These problems are solved through wireless communication between the industrial equipments. So, we propose wireless production process system based on IEEE 802.15.4 technology. It solves a complicated space and stops by replaced equipment in the factory. Then we simulated and analyzed IEEE 802.15.4 for Industrial Equipment based on Wireless Network.

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Production of Salidroside in Rhodiloa sachalinensis A. Bor Callus by the Elicitation and Precursor (참돌꽃 (Rhodiola sachalinensis A. Bor) 캘러스에서 elicitor와 전구체에 의한 Salidroside 생산성의 변화)

  • Lee, Jae-Seung;Kim, Min-Young;Kim, Jae-Heun;Nam, Jong-Hyun;Lee, Hyeon-Young;Hwang, Baik
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.4
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    • pp.268-272
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    • 2008
  • The effect of elicitor and precursor on salidroside production from Rhodiola sachalinensis A.Bor callus cultures was investigated. Callus cultures were treated with yeast extract, soft-ferrite ceramics powder, methyl jasmonate, ascorbic acid, jasmonic acid and $CuCl_2$/$CdCl_2$ as an elicitor. When callus cultures were treated with $0.2g/\ell$ of yeast extract, salidroside production from callus treated with yeast extract is 3.45 times higher than that of the controlled group. Among of them, callus cultures treated with yeast extract produced the highest salidroside. Callus cultures were treated with L-phenylalanine and L-tyrosine as a precursor for 4 days. The result of salidroside content analysis showed that all feeding of precursors not affected salidroside production from callus cultures. In case of L-tyrosine fed into callus cultures, both callus growth and salidroside production decreased at all concentrations.

Development of Multiple Wireless Communication Controller for Smart Factory Construction (스마트팩토리 구축을 위한 다중 무선통신 컨트롤러 개발)

  • Oh, Jae-Jun;Choi, Seong-Ju;Kim, Jin-Sa
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.30 no.9
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    • pp.602-608
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    • 2017
  • Due to recent industry 4.0, manufacturing has changed a lot. In particular, it is necessary to control the controller and controller of the control system, to communicate various production information and measurement information, and to produce a database in accordance with the flexible production for a small quantity of various items, and to manage the trend of major parts of production facilities. In this paper, we developed a multiple wireless communication controller for small scale control system for smart factory by applying XBee and microcomputer. This controller is cheap and easy to build multi-radio communication environment of 1: N and can control and monitor control system. In addition, we tested multiple wireless communication controllers by using signal processing device and C++, and constructed network, control, and database for mechanism module, and confirmed effectiveness for industrial application.

Dynamics of Plant Communities under Human Impact in the Green-Belt nearby Seoul -On the Production and Decomposition of Liffters in Grassland and Forests in Mt. Guryong- (人間干涉하의 首都圈 그린벨트 내 植物群集의 動態 - 九龍山의 草地와 森林에 있어서 落葉의 生産과 分解에 關한 硏究-)

  • Chang, Nam-Kee;Duck-Key Lee;Joon-Ho Kim
    • The Korean Journal of Ecology
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    • v.13 no.1
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    • pp.51-58
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    • 1990
  • The rates of litter production and decomposition of litters from grasslands and forests in Mt. Guryong were studied. The annual litter production of Glycine soja showed the highest value 1950.88 g/$m^2$/yr in the grasslands and that in Quercus acutissima, as 2202.38 g/$m^2$/yr in the forests. The highest decay rate of the grasslands was found in G. soja as k=0.713 and that of the forests was in Salix koreensis as k=0.319. The Z values of k in the grasslands was higher than that in the forests. The shortest half-time of the litter decay in the grasslands was 0.9 years in G. soja and the longest one of the forests was S. koreensis as 2.1 years and the longest of all was Q. mongolica as 5.2 years. In the amount of total nitrogen of litters, G. soja was the highest of the grasslands and S. koreensis was the highest of the forest. The content of the total nitrogen in litters was directly propotional to the decay rates.

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Evaluation of Biodiesel Production Systems and Factors Affecting Product Yield (바이오디젤의 생산 공정 비교 및 생성물 수율에 미치는 인자들)

  • Lee, Jong-Man;Lee, Jae-Heung;Cho, Nam-Jun
    • The Journal of Korean Institute for Practical Engineering Education
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    • v.3 no.1
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    • pp.183-192
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    • 2011
  • In recent years there has been an increasing focus on global warming and the exhaustion of resources caused by the heavy consumption of fossil fuels. In order to resolve these issues, biomass has gained much attention as a source of renewable energy. One area of particular interest has been the production of biodiesel. The biodiesel produced by the transesterification of vegetable oils, animal fats and waste cooking oils is expected to be one of the eco-friendly biomass-based alternatives to fossil fuels. This paper reviews some of the recent findings for the effective biodiesel production system, together with several factors affecting the biodiesel yield.

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A Study on the Production and Decomposition of Litters Related to Altitude (한라산, 소백산 및 태백산의 고도에 따른 낙엽의 생산과 분해에 관한 연구)

  • Chang, Nam Kee;Hi Chung Kwon
    • The Korean Journal of Ecology
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    • v.10 no.3
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    • pp.109-118
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    • 1987
  • The production and decomposition rates of litters were studied in three mountains, Mt. Halla, Mt. So-back and Mt. Tae-back. The amounts of N, P, K, Ca and Na in the soils were measured and the relationships between the mineral nutrients and decay rates were reserched, The annual litter production was the most as $1, 077.3g/m^2$ and the least as $248.0g/m^2$ in a stand of Quercus acutissima at 820m of the Mt. So-back and at 1, 350m of the Mt. So-back among the study sites, respectively. The decay rates of litter was the highest as k=0.448 and the lowest as k=0.082 in a stand of Q. acutissima at 820m of the Mt. So-back and at 1, 450m of the Mt. Tae-back at 1, 450m of the Mt. Tae-back among the study sites. The higher altitude was, the slower the decay rates were. The annual contents of mineral nutrient and their amounts inputted into the forest soil were proportional to the decay rate of organic metters.

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