• Natural Product Sciences
• /
• 제15권4호
• /
• pp.213-221
• /
• 2009

Longanae Arillus (the rind of fruits of Dimocarpus longan) has been consumed for the treatment of insomnia and anxiety in Asia. To provide further scientific basis to traditional uses of this fruit on insomnia, we evaluated the effects of methanol extract of Longanae Arillus (MELA) on the alteration of sleep architecture and electroencephalogram (EEG) power spectra in acutely and chronically restraint-stressed rats. Following postsurgical recovery, Polygraphic signs of sleep-wake activities were recorded for 24 h after MELA administration in rats. Rats in the acute stress and chronic stress were administered with MELA for 10 days. On the $8^{th},\;9^{th}\;and\;10^{th}$ day of MELA administration, the rats were stressed for 3 h once per day. On the $10^{th}$ day and 1 h after MELA administration, the rats were stressed once for 22 h in the chronic stress group. Acute and chronic stress induced alternations in cortex EEG recordings during non-rapid eye movement (NREM), rapid eye movement (REM) sleep and wakefulness. MELA shortened the total and REM sleep and increased the wakefulness in night time recording without changing daytime recordings. Chronic stress increased wakefulness and REM sleep, decreased total and NREM sleep in the daytime recording, and increased REM and decreased NREM sleep without changing total sleep and wakefulness in night time recording. These findings suggest that MELA ameliorated the alterations in REM and NREM sleep of acutely and chronically stressed rats via modulation of cortical ${\alpha}-$, ${\theta}-$ and ${\delta}-$ wave activity.

• 한국펄프종이공학회:학술대회논문집
• /
• 한국펄프종이공학회 2007년도 제32회 펄프종이기술 국제세미나
• /
• pp.83-125
• /
• 2007

Increasing energy costs have caused profitability problems for paper suppliers. Therefore unprofitable lines are being closed down. The actions aiming for improved profits are focused either on cost savings or on increasing the capacity of the remaining machines. The runnability of a paper machine and its total efficiency have a significant effect on energy consumption. Producing one ton of waste paper consumes at least as much energy as producing the same amount of sellable end product. New automation solutions enable significant cost-effective improvements to the total efficiency of a line without large investment projects. The measures focus on minimizing changes, interruptions, interruption recovery times and grade change times. Newest actuators, online quality measurements and wet end analysators create an improvement potential, which can be optimally implemented with the latest machine direction control solutions, based on model predictive control concepts. Equally, drying management is significant to the energy consumption. The newest control strategies optimize the use of various drying actuators for different situations; either by responding to changes as efficiently as possible or by using only the cheapest energy sources in stable situations. An even steam supply, which is vital for paper machines, is achieved with control for the power plant steam network. This makes possible to avoid the delays upon starting the paper machine and assure an even steam supply for the drying section and the actuators. This document describes means which have brought significant energy and raw material savings for paper machines. Metso Automation has provided efficiency improvement packages, which are usually based on optimized control of dry weight and drying in all running conditions. The solutions are based on performance analysis, on which the estimations for improvement potential and the necessary actions are based on. Typically benefits on an annual level have been from hundreds of thousands of euros to over one million euro. For example, variations in dry weight have been decreased more than 50%. The results are presented with a few examples. Additionally, the analysis models, adjustment solutions and the changes in running methods with which the results were achieved, are presented.

• 분석과학
• /
• 제33권1호
• /
• pp.42-48
• /
• 2020

Bromate is a disinfection by-product generated mainly from the oxidation of bromide during the ozonation and disinfection process in order to remove pathogenic microorganism of drinking water, and classified as a possible human carcinogen by International Agency for Research of Cancer (IARC) and World Health Organization (WHO). For the purpose of determining the trace level concentration of bromate, several sensitive techniques are applied mostly based on suppressed conductivity detection and UV/Visible detection after postcolumn reaction (PCR). In this study, the suppressed conductivity detection method and the PCR-UV/Visible detection method through the triiodide reaction were compared to analyze the trace bromate in water samples and estimated for the availability of these analytical methods. In addtion, the state-of-the-art techniques was applied for the determination of trace level bromate in various water matrices, i.e., soft drinking water, hard drinking water, mineral water, swimming pool water, and raw water. In comparison of two analytical methods, it was found that the conductivity detection had the suitable advantage to simultaneously analyze bromate and inorganic anions, however, the bromate might not be precisely quantified due to the matrix effect especially by chloride ion. On the other hand, the trace bromate was analyzed effectively by the method of PCR-UV/Visible detection through triiodide reaction to satisfactorily minimize the matrix interference of chloride ion in various water samples, showing the good linearity and reproducibility. Furthermore, the method detection limit (MDL) and recovery were 0.161 ㎍/L and 101.0-108.1 %, respectively, with a better availability compared to conductivity detection.

• Journal of Microbiology and Biotechnology
• /
• 제23권5호
• /
• pp.587-601
• /
• 2013

During the last few decades, it has become evident that the compatibility of the yeast biochemical environment with the ability to process and translate the RNA transcript, along with its capacity to modify a translated protein, are relevant requirements for selecting this host cell for protein expression in several pharmaceutical and clinical applications. In particular, Pichia pastoris is used as an industrial host for recombinant protein and metabolite production, showing a powerful capacity to meet required biomolecular target production levels in high-throughput assays for functional genomics and drug screening. In addition, there is a great advantage to using P. pastoris for protein secretion, even at high molecular weights, since the recovery and purification steps are simplified owing to relatively low levels of endogenous proteins in the extracellular medium. Clearly, no single microexpression system can provide all of the desired properties for human protein production. Moreover, chemical and physical bioprocess parameters, including culture medium formulation, temperature, pH, agitation, aeration rates, induction, and feeding strategies, can highly influence product yield and quality. In order to benefit from the currently available wide range of biosynthesis strategies using P. pastoris, this mini review focuses on the developments and technological fermentation achievements, providing both a comparative and an overall integration analysis. The main aim is to highlight the relevance and versatility of the P. pastoris biosystem to the design of more cost-effective microfactories to meet the increasing demands for recombinant membrane proteins and clinical antibodies for several therapeutic applications.

• 대한한의학회지
• /
• 제29권4호
• /
• pp.133-145
• /
• 2008

Objectives: The aim of present study was to investigate recovery effects of Hirudo, which has been used clinically in diabetes therapy. Methods: We established three groups: normal, control, Hirudo, and assigned 6 rats to each group. The normal group was not treated by any process and fed by normal saline. The control & Hirudo groups were administered streptozotocin (STZ) to induce diabetes. Hirudo extract was orally administered to the Hirudo group for 10 days. After 8 weeks, the rats were sacrificed and their body weight, 24hrs urinary protein excretion, glucose, albumin, BUN, creatinine, total-cholesterol, LDL-cholesterol, triglyceride in blood, and level of glycation end-product (AGE) and transforming growth factor (TGF-${\beta}1$) in serum were measured. Morphological profiles and morphometric studies of the kidney cortex, renal transforming growth factor (TGF-${\beta}1$) expression, and renal receptor for advanced glycation end-products (RAGE) expression were studied. Results: The following results were obtained. The protein amount in urine per 24hrs of the Hirudo-treated group as compared to the control group was significantly reduced. The BUN and creatinine level in serum of the Hirudo-treated group as compared to the control group was significantly inhibited. The construction change in kidney of the Hirudo-treated group as compared to the control group was significantly inhibited. The factor of the Hirudo-treated group as compared to the control group was significantly inhibited, which induced the construction change in kidney. Conclusions: The above results suggest that Hirudo partially improved the function of the kidney.

• 한국생활과학회지
• /
• 제24권1호
• /
• pp.97-109
• /
• 2015

Recently, because the market for compression wear now includes all consumers, not just professionals, various items for recovery after exercising or for enhanced effects from exercise have been introduced. In this research, a systematic and stepwise design process was proposed to develop compression garment that has both functional area and appropriate pressure to protect the knee when exercising. The U-V format functional area that wraps underneath the knee was selected by considering the shape and change in the skin length when bending the knee. After the selection of the functional area, a total of seven knee design areas, including the existing product, were designed to determine the appropriate pressure. After various movements, the compression garment was ranked in terms of support of the knee, level of pressure, discomfort of seam line, and comfort of popliteal; the preferred design was selected using the quad method. Four compression wear garments were produced using two selected preferred designs; the wear evaluation was performed using a seven-point Likert scale. As a result, the optimal reduction rate of the pattern was calculated based on Ziegert and Keil's method. The applied percentage of the fabric stretch at the upper part of the crotch was 66% for the width and 50% for the length; for the lower part of the crotch, only 66% for the width was applied. Moreover, it was determined that the design of the U-V knee protection part was preferred when a 7 mm square was placed at a 1 mm distance because this not only supports the knee but also allows the fabric to accommodate various skin deformations.

• 자원리싸이클링
• /
• 제11권1호
• /
• pp.9-18
• /
• 2002

폐 컴퓨터 기판(Printed Wiring Boards, PWBs)은 다양한 종류의 금속 및 합금류, 각종의 유기 및 무기화합물 등으로 구성되어 있다. 따라서 폐 기판에 포함된 유가물을 경제적인 방법으로 분리 회수하면 2차 자원으로써 활용가치가 매우 클 것으로 판단되어, 물리적인 선별기술에 의하여 유가금속을 분리 회수하는 데에 따른 특성 연구를 행하였다. 본 연구에는 폐 컴퓨터 기판에 탑재된 소켓류와 칩류를 탈리한 다음 소켓류, 칩류 및 보드류로 분리하여 각각의 산출물의 특성에 따른 적절한 물리적 분리선별 기술을 적용하였다. 소켓류를 파쇄하여 입자크기를 -2.36 mm/+1.18 mm 범위로 조절한 다음 건식 자력선별을 실시하였을 때, 자성산물의 약 97 wt%가 금속류였다. 칩류의 경우에는 -2.36 mm1+0.15 nun의 크기로 분쇄하고 공기분급 및 건 식 자력선별을 행하여 Fe-Ni 97%, Cu 95%를 각각 회수할 수 있었다. 보드류의 경우에는 금속류가 얇게 프린트 된 상태이기 때문에 가능한 단체분리 효과를 향상시키기 위하여 ball mill로 분쇄하였으며, 공기 분급기에 의한 정밀 분급을 행하여 Cu 77%를 회수할 수 있었다.

• 자원리싸이클링
• /
• 제15권2호
• /
• pp.37-44
• /
• 2006

국내 흑연광의 총 매장량은 약 260만 톤 정도이지만, 국제경쟁력을 가지지 못해 현재는 일부 광산에서만 채광하고 있다. 그러나 최근 휴대용 전자제품의 수요가 증가함에 따라 2차 전지의 전극에 사용되는 고순도의 흑연을 전량 고가로 수입에 의존하고 있어, 고순도 흑연의 국산화를 위한 연구가 필요한 실정이다. 그러므로 본 연구에서는 고순도 흑연을 생산하기 위한 전처리 단계로서 29.50% F.C.의 원광($D_{50}=69.393{\mu}m$)시료를 attrition mill에서 20분 마광($D_{50}=10.314{\mu}m$)하여 microbubble column을 이용하여 실험한 결과 정선과정 없이도 95% F.C. 이상의 산물을 90%이상의 회수율로 얻을 수 있었다.

• 한국식품저장유통학회지
• /
• 제5권3호
• /
• pp.239-246
• /
• 1998

폴리이미드 고분자막을 사용한 질소부하 장치를 설계하고, 이를 CA 저장에 활용하기 위한 기술적 기본 데이터를 제시하였다. 순수 산소 및 질소의 투과특성은 dual-mode sorption 모델로서 설명될 수 있었으며, 공기 중의 산소의 투과율은 공기 중에 존재하는 질소의 영향으로 순수 산소의 투과율에 비해 크게 감소하나, 공기 중의 질소의 투과율은 산소의 영향으로 순수 질소의 투과율에 비해 증가하였다. 수행한 압력 및 온도 범위 내에서 이상분리인자는 5에서 6 사이의 값을 나타내었으며, 공기의 분리인자는 이상분리인자보다 작은 값을 나타내었다. 산소의 투과활성화에너지가 질소의 투과 활성화에너지보다 커서 이상분리인자는 온도가 증가함에 따라 증가하였다. 제품회수율이 증가함에 따라 농축부의 질소농도는 급격히 감소하였으며, 이로부터 제품회수율은 CA 저장고에서 요구되는 질소농도를 얻기 위한 주요 조업인자임을 알 수 있었다. Polyimide 막 시스템을 이용한 CA 저장 공정의 가상적인 모형을 설정하고, 시스템의 조업시간에 따른 저장고의 질소농도를 예측할 수 있는 관계식을 제시하였다.

• 대한본초학회지
• /
• 제21권2호
• /
• pp.9-13
• /
• 2006

Objectives : To determine the DNA sequence of the 18S rRNA gene of the Rehmannia glutinosa and analyze it phylogenetically Methods : Dried root of the Rehmannia glutinosa was ground with a mortar and pestle. Glass beads(0.5 mm in diameter), TE buffer and SDS solution were added to that. The mixture was vortexed vigorously and extracted with the mixture of phenol, chloroform and isoamyl alcohol and with the mixture of the chloroform and isoamyl alcohol. The nucleic acids were precipitated with ethanol and resuspended in TE buffer. Contaminating RNA was digested with RNAse A and the DNA was purified further with the Geneclean Turbo Kit. This DNA was used as a template for amplification of the 18S rRNA gene by PCR. The PCR product was cloned in the pBluescript SK II plasmid by blunt-end ligation and the DNA sequence of the insert was determined. This DNA sequence was analyzed phylogenetically by the BLAST program. Results and Conclusion : Vortexing the ground powder of the dried plant root with glass beads during cell lysis improved recovery of DNA. The DNA sequence of the Rehmannia glutinosa 18S rRNA gene was determined and deposited at the GenBank as the accession number DQ469606. Phylogenetic analysis of that sequence showed the relationship between the members of the family of Scrophulariaceae and also the close relationship of the Buddleja davidii to the members of the Scrophulariaceae family.