• Natural Product Sciences
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• 제18권1호
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• pp.60-66
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• 2012

Human skin is the first line of defense for the protection of the internal organs of the body from different stimuli. Ultraviolet B (UVB) irradiation induces skin damage and inflammation through the secretion of various cytokines, which are immune regulators produced by cells. To prevent the initiation of skin inflammation, keratinocytes that have been irreversibly damaged by radiation must be removed through the apoptotic mechanism. Ixeris dentata (family: Asteraceae) is a perennial medicinal herb indigenous to Korea. It has been used in Korea, China, and Japan to treat in digestion, pneumonia, diabetes, hepatitis, and tumors. To gain insight into the anti-inflammatory effects of I. dentata, we examined its influence on UVB-induced pro-inflammatory cytokine production in human keratinocytes (HaCaT cells), by observing cells that were stimulated with UVB in the presence or absence of I. dentata. In the present study, pro-inflammatory cytokine production was determined by performing enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, and western blot analysis to measure the activation of mitogen-activated protein kinase (MAPKs). I. dentata inhibited UVBinduced production of the pro-inflammatory cytokine interleukin (IL)-6 in a dose-dependent manner. Further, I. dentata inhibited the UVB-induced expression of cyclooxygenase (COX)-2. Furthermore, I. dentata inhibited the phosphorylation of c-Jun NH2-terminal kinase and p38 MAPKs, suggesting that it inhibits the secretion of the pro-inflammatory cytokines IL-6 and IL-8, and COX-2 expression, by blocking MAPK phosphorylation. These results suggest that I. dentate can potentially protect against UVB-induced skin inflammation.

• 경영과정보연구
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• 제23권
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• pp.25-40
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• 2007

In the highly competitive and divers world of financial market, customer is the single most important factor to company's survival. Especially, creating a relationship with valued customers is a key to success. CRM provides the mean to retain high value customers. It takes a prospect of what customers expect. Utilizing those knowledge can help the products and service meet the customers' needs, thereby maximizing customer satisfaction and company's profit. In this report, I am going to suggest a few ways to develop successful CRM in the life insurance industry. First, CRM should innovate the way of communication to keep pace with Web 2.0 era. In other words, the customer's needs should be caught by real-time communication than traditional off-line market research. Thus, the functionality and specification of products can be decided by customer's direct choice so that the customers are able to purchase the understanding and experience of the products. Second, CRM project should consider whether the initial strategy plan can promise the stable growth of customer at the first step. When planning strategy, the project needs to identify what customer wants and how to fulfill the needs with stable growth of the customer. In addition, the CRM should be developed by realizing that customer centric benefits ultimately guarantee the growth of the organization. Third, CRM systems should enhance the organization's ability to take the customer's insight in a 360 degree view and to capture the voice of the customer directly. In order to develop the best matched product package, more precise customer segmentation should be ahead of market segmentation strategy. Forth, the biggest reward from CRM will be a customer royalty program. Many successful banks are already planning and practicing customer royalty strategy. A comprehensive analysis of customers and their behavior allow organization to identify high value potential customers' needs and determine a strategy required to meet those needs. Even life insurance companies such as Prudential Korea are developing products designed for royal customers. Fifth, understanding and managing the experience of customer called Customer Experience Management also can increase customer satisfaction. Measuring only customers' experience and adapting it to marketing strategy make products position in the gap between the customers' expectation and experience not required by market. A key component of CEM is its application across all organizational functions. At last, the direction of change and development of CRM can be defined from the conceptualization of information technology represented by Ubiquitous and Web 2.0. Instead of just managing customer information, companies should take the initiative in personalized system with customer oriented strategy. Furthermore, with the regular communication between CRM stakeholders (Sales-Marketing-IT), customer's demand should be directly reflected to enterprise strategy in real time.

• 디지털융복합연구
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• 제15권8호
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• pp.333-340
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• 2017

타임랩스는 일정하게 정해진 간격으로 움직임을 촬영한 후 정상 속도로 영사하는 영상 기법이다. 글로벌 브랜드의 타임랩스 광고 영상을 살펴보고, 새로운 영상기법인 타임랩스의 구성요소와 표현방식을 분석하는 모델을 제시하고자 하였다. 선행연구로 문헌연구와 인터넷 자료조사, 유투브 영상자료 등을 조사하였다. 연속촬영이 영상기법으로 발전하면서 국내외 다큐멘터리, 국내외 드라마, 영화, 광고 등에 적용된 제작 현황을 살펴보았다. 2015-2016년 최근 광고에 타임랩스 기법이 사용된 아이폰광고(2016년), 랄프로렌 폴로광고(2015년), 캐논EOS(2013년) 영상의 기법을 분석한다. 타임랩스 구성요소는 정적요소로 정적모티브는 주로 인위적인 구조물이었고, 장소는 야외이며, 컬러는 장소의 특성을 잘 보여주는 시간에 촬영되었으며, 레이아웃은 모두 중앙에 배치 하였다. 동적요소로 동적 모티브는 움직이는 대상이며, 동선은 대상에 따른 스토리로 구성되었고, 시간은 짧게는 11-15초, 길게는 1분 30초 정도이며, 편집은 주로 브랜드 로고가 강조된 제품 중심이었다. 결론적으로 광고에 주목하게 하고 눈길을 사로잡는 것이 영상의 역할이다. 구매자의 마음을 움직이게 하는 데는 마음속에 내재된 감정을 유도하여 비언어적 기호인 영상으로 자극하는 타임랩스와 같은 연출과 편집이 필요하다. 향후 연구는 영상의 시간적 편집에 관한 다양한 시도가 나타날 것으로 보인다.

• Journal of Ginseng Research
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• 제42권1호
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• pp.42-49
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• 2018

Background: Ginsenoside F1 has been described to possess skin-whitening effects on humans. We aimed to synthesize a new ginsenoside derivative from F1 and investigate its cytotoxicity and melanogenesis inhibitory activity in B16BL6 cells using recombinant glycosyltransferase enzyme. Glycosylation has the advantage of synthesizing rare chemical compounds from common compounds with great ease. Methods: UDP-glycosyltransferase (BSGT1) gene from Bacillus subtilis was selected for cloning. The recombinant glycosyltransferase enzyme was purified, characterized, and utilized to enzymatically transform F1 into its derivative. The new product was characterized by NMR techniques and evaluated by MTT, melanin count, and tyrosinase inhibition assay. Results: The new derivative was identified as (20S)-$3{\beta},6{\alpha},12{\beta}$,20-tetrahydroxydammar-24-ene-20-O-${\beta}$-D-glucopyranosyl-3-O-${\beta}$-D-glucopyranoside(ginsenoside Ia), which possesses an additional glucose linked into the C-3 position of substrate F1. Ia had been previously reported; however, no in vitro biological activity was further examined. This study focused on the mass production of arduous ginsenoside Ia from accessible F1 and its inhibitory effect of melanogenesis in B16BL6 cells. Ia showed greater inhibition of melanin and tyrosinase at $100{\mu}mol/L$ than F1 and arbutin. These results suggested that Ia decreased cellular melanin synthesis in B16BL6 cells through downregulation of tyrosinase activity. Conclusion: To our knowledge, this is the first study to report on the mass production of rare ginsenoside Ia from F1 using recombinant UDP-glycosyltransferase isolated from B. subtillis and its superior melanogenesis inhibitory activity in B16BL6 cells as compared to its precursor. In brief, ginsenoside Ia can be applied for further study in cosmetics.

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2010년도 춘계학술발표대회 초록집
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• pp.51-51
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• 2010

Recently just as in the automobile industry, shipbuilders also try to reduce material consumption and weight in order to keep operating costs as low as possible and improve the speed of production. Naturally industry is ever searching for welding techniques offering higher power, higher productivity and a better quality. Therefore it is important to have a details research based on the various welding process applied to steel and other materials, and to have the ability both to counsel interested companies and to evaluate the feasibility of implementation of this process. Submerged-arc welding (SAW) process is usually used about 20% of shipbuilding. Similar to gas metal arc welding(GMAW), SAW involves formation of an arc between a continuously-fed bare wire electrode and the work-piece. The process uses a flux to generate protective gases and slag, and to add alloying elements to the weld pool and a shielding gas is not required. Prior to welding, a thin layer of flux powder is placed on the work-piece surface. The arc moves along the joint line and as it does so, excess flux is recycled via a hopper. Remaining fused slag layers can be easily removed after welding. As the arc is completely covered by the flux layer, heat loss is extremely low. This produces a thermal efficiency as high as 60% (compared with 25% for manual metal arc). SAW process offers many advantages compared to conventional CO2 welding process. The main advantages of SAW are higher welding speed, facility of workers, less deformation and better than bead shape & strength of welded joint because there is no visible arc light, welding is spatter-free, fully-mechanized or automatic process, high travel speed, and depth of penetration and chemical composition of the deposited weld metal. However it is difficult to application of thin plate according to high heat input. So this paper has been focused on application of the field according to SAW process for thin plate in ship-structures. For this purpose, It has been decided to optimized welding condition by experiments, relationship between welding parameters and bead shapes, mechanical test such as tensile and bending. Also finite element(FE) based numerical comparison of thermal history and welding residual stress in A-grade 3.2 thickness steel of SAW been made in this study. From the result of this study, It makes substantial saving of time and manufacturing cost and raises the quality of product.

• BMB Reports
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• 제31권6호
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• pp.578-584
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• 1998

In a previous paper (Kim et al., 1996a), the immediate 5' -flanking region and coding region of the human UDP-N -acetylglucosamine:-D-mannoside-1,4-Nacetylglucosaminyltransferase III (N-acetylglucosaminyitransferase- III; GnT-III) gene was reported, isolated and analyzed. Herein, we report on amplification of a new 5' -noncoding region of the GnT-III mRNA by single-strand ligation to single-stranded cDNA-PCR (5' -RACE PCR) using poly(A)+ RNA isolated from human fetal liver cells. A cDNA clone was obtained with 5' sequences (96 bp) that diverged seven nucleotides upstream from the ATG (+1) start codon. A concensus splice junction sequence, TCTCCCGCAG, was found immediately 5' to the position where the sequences of the cDNA diverged. The result suggested the presence of an intron in the 5' -noncoding region and that the cDNA was an incompletely reversetranscribed cDNA product derived from an mRNA containing a new noncoding exon. When mRNA expression of GnT-III in various human tissues and cancer cell lines was examined, Northern blot analysis indicated high expression levels of GnT-III in human fetal kidney and brain tissues, as well as for a number of leukemia and lymphoma cancer cell lines. Promoter activities of the 5' -flanking regions of exon 1 and the new noncoding region were measured in a human hepatoma cell line, HepG2, by luciferase assays. The 5'-flanking region of exon 1 was the most active, whilst that of exon 2 was inactive.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.769-773
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• 2014

Fangchinoline (Fan) inhibits cell proliferation and induces apoptosis in several cancer cell lines. The effects of Fan on cell growth and proliferation in breast cancer cells remain to be elucidated. Here, we show that Fan inhibited cell proliferation in the MDA-MB-231 breast cancer cell line through suppression of the AKT/Gsk-3beta/cyclin D1 signaling pathway. Furthermore, Fan induced apoptosis by increasing the expression of Bax (relative to Bcl-2), active caspase 3 and cytochrome-c. Fan significantly inhibited cell proliferation of MDA-MB-231 cells in a concentration and time dependent manner as determined by MTT assay. Flow cytometry analysis demonstrated that Fan treatment of MDA-MB-231 cells resulted in cell cycle arrest at the G1 phase, which correlated with apparent downregulation of both mRNA and protein levels of both PCNA and cyclin D1. Further analysis demonstrated that Fan decreased the phosphorylation of AKT and GSK-3beta. In addition, Fan up-regulated active caspase3, cytochrome-c protein levels and the ratio of Bax/Bcl-2, accompanied by apoptosis. Taken together, these results suggest that Fan is a potential natural product for the treatment of breast cancer.

• 지적과 국토정보
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• 제47권2호
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• pp.185-199
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• 2017

본 연구는 예측하기 어려운 미래의 다양한 수요를 수용하기 위하여 재조합방법을 통한 공간정보제품 제작방법을 제안한다. 현재의 측량중심의 공간정보제품의 생산방안은 특정 지도제품 중심의 Top-down방식이므로 공간정보의 활용 확산 관점에서는 한계점을 가지고 있다. 이는 불특정 다수를 위한 공간정보의 활용성 관점보다는 모두를 위한 지도라는 관점에서 다양한 수요를 만족시키기에는 한계가 있다. 이런 한계점을 해결을 위해 사용자 맞춤형의 Bottom-up방식 공간정보 생산과 재조합을 통한 공간정보 제품 제작이 요구되고 있다. 이를 위해 본 논문은 기존의 Top-down 방식의 결과인 수치지도와 재조합을 통한 공간정보제품의 제작에 대한 이론적 고찰과 비교를 통해 보완 방안을 제안하였다. 이는 공간정보를 기초속성을 가지는 형태와 관계된 속성정보를 가지는 데이터로 재분류하고 각 데이터에 관계성 및 관리에 관한 속성을 부여하는 방안이다. 이 방법의 실효성 증명을 위해 기존 수요의 만족 가능성과 미래 수요의 대응 가능성을 중심으로 적용사례를 도출하였다, 그리고 기존의 국가기본도, 주제도, 향후 수요 예측 제품의 해당 대표제품을 제작하여 확인하였다.

• 한국산학기술학회논문지
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• 제19권9호
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• pp.261-268
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• 2018

전력수요와 부하용량이 증가 되어감에 따라 부하에 전력공급을 위한 간선으로서의 케이블은 설치 시간의 과다와 공간의 제약으로 사용이 제한적이다. 이런 문제를 해결하기 위한 간선 시스템으로 대전류를 효율적으로 공급해 줄 수 있는 버스웨이 시스템의 사용이 증가되고 있으나, 신뢰성의 검증을 위한 시험평가 방법과 그에 대한 결과 제시가 부족한 실정이다. 본 논문에서는 에폭시를 주절연으로 하는 버스웨이 시스템의 신뢰성 검증을 위해서 IEC 61439-6에서 제시된 시험항목이외에 장기 사용성과 신뢰성을 평가할 수 있는 시험항목을 선정하였다. 열 및 전기적 스트레스를 동시에 인가하여 최대한 실사용 상태를 반영한 복합가속열화시험을 실시하였으며, 50년 수명 조건을 충족하도록 열화 조건을 선정하여 버스웨이 시스템의 절연성능 확인시험을 통해 수명을 만족함을 확인하였다. 또한, 버스웨이가 설치되는 온도, 습도 및 부하전류 사용 환경에 대한 적합성 확인에 대한 신뢰성 평가 시험항목으로서 열적 과전류 시험, 침수시험, 냉충격시험, 온도 사이클 시험을 선정하여 실시하였으며, 확인 시험을 통해 시험 전후의 특성 변화와 이상 유무를 검토하였다. 이 결과들로부터 에폭시 절연형 버스웨이 시스템의 평가항목으로서 본 논문에서 제시한 시험항목이 적절하고, 그에 대한 제품의 성능도 우수함을 확인하였다.

• 생명과학회지
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• 제19권12호
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• pp.1808-1814
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• 2009

방사선에 대한 방호와 면역기능 조절을 목적으로 새로운 생약복합조성물인 HemoHIM을 개발하였다. 식품 원료로 사용 가능한 생약재 3종 당귀, 천궁, 백작약의 에탄올 분획을 열수추출물에 첨가하여 HemoHIM을 제조하였다. HemoHIM의 항알레르기 효과를 검증하기 위하여 사람 비만세포주 HMC-1을 사용해 compound 48/80으로 유도되는 히스타민 분비량과 PMA/A23187로 유도되는 염증성 사이토카인의 분비량을 측정하였다. 히스타민의 양은 형광분석법으로, 염증성 사이토카인 IL-6, IL-8, TNF-$\alpha$, GM-CSF의 양은 효소결합 면역측정법으로 측정하였다. 저농도의 HemoHIM에 의해 히스타민 분비량이 억제되었고, 모든 농도에서 IL-6, TNF-$\alpha$, GM-CSF의 분비량은 억제되었지만 IL-8은 고농도에서만 억제되었다. 사이토카인의 mRNA 발현량은 HemoHIM의 농도 의존적으로 억제되었다. 그리고 c-kit와 Fc$\varepsilon$RI의 mRNA 발현량도 모든 농도에서 억제되었지만, tryptase의 mRNA 발현량은 저 농도에서만 억제되었다. 이상의 결과로 HemoHIM이 비만세포의 활성을 억제하는 효과가 있다는 것을 알 수 있었으며, 상대적으로 독성이 적은 항알레르기 제재로 개발할 가능성을 제시한 것으로 생각된다.