• 한국패류학회지
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• 제27권4호
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• pp.387-393
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• 2011

The objective of this study was to develop a real-time PCR method for the rapid detection and quantification of the protozoan pathogen Perkinsus olseni using a TaqMan probe. For the standard, genomic DNA was extracted from $10^5$ in vitro-cultured P. olseni trophozoites, and then 10-fold serial dilutions to the level of a single cell were prepared. To test the reliability of the technique, triplicates of genomic DNA were extracted from $5{\times}10^4$ cells and 10-fold serial dilutions to the level of 5 cells were prepared. The standards and samples were analyzed in duplicate using an $Exicycler^{TM}$ 96 real-time quantitative thermal block. For quantification, the threshold cycle ($C_T$) values of samples were compared with those obtained from standard dilutions. There was a strong linear relationship between the $C_T$ value and the log concentration of cells in the standard ($r^2$ = 0.996). Detection of DNA at a concentration as low as the equivalent of a single cell showed that the assay was sensitive enough to detect a single cell of P. olseni. The estimated number of P. olseni cells was similar to the original cell concentrations, indicating the reliability of P. olseni quantification by real-time PCR. Accordingly, the designed primers and probe may be used for the rapid detection and quantification of P. olseni from clam tissue, environmental water, and sediment samples.

• 접착 및 계면
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• 제8권2호
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• pp.15-21
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• 2007

광기능성시트 보호용 점착제를 제조하기 위하여 천연고무와 DCPD계 점착부여수지, 세 종류의 지방족 점착부여수지를 이용하여 천연고무계 점착제를 제조하였다. 또한, 천연고무의 낮은 응집력을 대체하기 위하여 점착부여수지의 함량이 고정된 상태에서 SIS 블록공중합체를 함량비에 따라 첨가하여 점착제를 제조하였다. 제조한 점착제의 점착물성을 초기점착력과 박리강도로써 평가하였다. 천연고무/점착부여수지의 초기점착력은 점착부여수지의 함량이 증가함에 따라 증가하다 최대값을 보이고 감소하였다. 또한, 지방족계 점착부여수지의 연화점이 증가함에 따라 초기점착력이 다소 증가하였다. 점착부여수지의 함량이 50 wt%까지는 박리강도가 증가하였으나 그 이상의 함량에서는 혼합파괴 현상을 보였다. SIS가 블랜드된 점착제의 초기점착력은 지방족계 점착부여수지의 함량이 20~40 wt%에서 최대값을 보였으나 20 wt%에서 fibrillation 현상을 나타내었고 박리강도는 40 wt%에서 최대값을 나타내었다.

• Korean Membrane Journal
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• 제6권1호
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• pp.16-23
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• 2004

The silica containing carbon (C-SiO$_2$) membranes were fabricated using poly(imide siloxane) (PIS) having -CO- swivel group. The characteristics of porous C-SiO$_2$ structures prepared by the pyrolysis of poly(imide siloxane) were related with the micro-phase separation between the imide block and the siloxane block. Furthermore, the nitrogen adsorption isotherms of the CMS and the C-SiO$_2$ membranes were investigated to define the characteristics of porous structures. The C-SiO$_2$ membranes derived from PIS showed the type IV isotherm and possessed the hysteresis loop, which was associated with the mesoporous carbon structures, while the CMS membranes derived from PI showed the type I isotherm. For the molecular sieving probe, the C-SiO$_2$ membranes pyrolyzed at 550, 600, and 700$^{\circ}C$ showed the O$_2$ permeability of 924, 1076, and 367 Barrer (1 ${\times}$ 10$\^$-10/㎤(STP)cm/$\textrm{cm}^2$$.$s$.$cmHg) and O$_2$/N$_2$ selectivity of 9, 8, and 12.

• The Korean Journal of Pain
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• 제9권2호
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• pp.340-343
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• 1996

Background: In our hospital, stellate ganglion block(SGB) has been performed for the prevention and treatment of vasospasm after microscopic reimplantation of finger(s). If brachial plexus block(BPB) has the same effect of sympathetic block on the upper extremity as SGB, it may be preferable to the SGB because it povides postoperative analgesia and is administered continuously. So we measured and compared the change of skin temperature on the forearm as the parameter of sympathetic blockade after SGB and BPB. Methods: The forty-two patients, belonged to ASA class 1~2, were received BPB for hand surgery. The skin temperature was measured before and after BPB on the forearm with patient monitor(LN 6199, YSI 400 Series Temperature Probe, Hellige, Germany). After 24 hours, ipsilateral SGB was performed and skin temperature was recorded before and after SGB. Results: The increase of skin temperature after procedures was $1.1{\pm}0.5^{\circ}C$(from $34.5{\pm}0.7^{\circ}C$ to $35.6{\pm}0.5^{\circ}C$) in BPB and $0.6{\pm}0.3^{\circ}C$(from $34.9{\pm}0.5^{\circ}C$ to $35.5{\pm}0.5^{\circ}C$) in SGB. The changes of skin temperature in both blocks were statistically significant(p<0.01), and the skin temperatures after each procedure were revealed no significant difference(p$\simeq$0.62). Conclusion: We thought that BPB produced sympathetic blockade on the upper extremity as much as SGB. Moreover, it provides postoperative pain relief and may be employed as continuous BPB could be used for hand surgery with many advantages.

• Archives of Plastic Surgery
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• 제36권2호
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• pp.205-210
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• 2009

Purpose: Corrugator supercilii muscle pulls eyebrow to inferomedial direction and produces the vertical component of the glabellar line formation. Current techniques for eliminating of glabellar frown include direct resection of corrugators and botulinum toxin injection. Muscle resection in endoscopic face lift procedure is relatively complex and has many disadvantages ranging from possible nerve injury, postoperative edema, pain and a long recovery period. The Botox treatment on the other hand is much more simple in technique but has a short duration of action. The authors have attempted new ways of finding improved treatment of the glabellar frown by selectively blocking of motor nerves innervating the corrugator supercili muscle by using radiofrequency ablation technique. Methods: A total of 80 patients were recruited in our study during the period between Feb. 2007 to June 2008. A probe was introduced from the supraorbital ridge and advanced to the corrugator supercilii muscle. Nerve stimulator was then used to locate the nerve innervating the corrugator and radiofrequency ablation of the nerve was done. Results: In all patients, there were marked improvement in glabellar frown after treatment. There were no reported cases of any relapses during the follow up period. No complication was noted such as facial nerve injury. No patient complained of any adverse symptoms other than slight discomfort due to swelling of the operation site. Conclusion: The treatment of glabellar frown lines using selective nerve block with radiofrequency ablation was not only less invasive but also excellent in surgical outcomes.

• 대한정형외과 초음파학회지
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• 제7권2호
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• pp.105-112
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• 2014

목적: 미추 경막외 차단술은 요통과 하지 방사통에 효과적으로 외래환자의 치료에 자주 사용되고 있으나 임상 경험이 풍부한 의사에 의해 시술되어도 25%의 실패율이 보고되고 있다. 저자들은 초음파 유도하 미추 경막외 차단술의 유효성을 추간판 탈출증 환자군과 척추관 협착증 환자군으로 나누어 결과를 비교하였다. 대상 및 방법: 요통과 하지 방사통을 호소하여 본원 외래에 내원한 55명의 환자를 대상으로 초음파 유도하 미추 경막외 차단술을 시행하였다. 환자를 복와위 자세로 눕힌 후 방사형 탐지자(round probe)를 이용하여 22게이지 바늘이 천-미추막을 통과한 것을 확인 후 약물을 투여하였다. 추간판 탈출증 환자군은 31명이었으며, 척추관 협착증 환자군은 24명이었다. 임상적 평가는 시각통증척도(VAS)를 이용하여 통증의 변화를 평가하였으며, 시행 전, 시행 후, 시행 2주 후, 시행 4주 후 전화 인터뷰와 외래 방문 시 조사하였다. 결과: 초음파 유도하 미추 경막외 차단술은 55예 중 53예에서 바늘이 성공적으로 삽입되어 96.4%의 성공률을 보였다. 추간판 탈출증 환자군과 척추관 협착증 환자군에서 성별의 차이는 없었으나 나이는 추간판 탈출증군에서 $42.3{\pm}10.8$세로 척추관 협착증군의 $62.8{\pm}15.1$세와 비교할 때 유의한 차이가 있었다(p<0.001). 시각통증척도는 추간판 탈출증 군에서 시행 전 6.8, 시행 후 3.1, 시행 2주 후 1.8, 시행 4주 후 1.77로 나타났으며, 척추관 협착증 군에서 시행 전6.9, 시행 후 3.6, 시행 2주 후 4.3, 시행 4주 후 4.9로 나타났다. 시행 전에 비해 시행 후 시각통증척도는 양 군에서 유의하게 호전되었으며(p<0.001), 나이를 보정한 후의 결과, 시간에 따라 양 군간 시각통증척도의 차이는 통계적으로 유의하였다(p<0.001). 결론: 초음파 유도하 미추 경막외 차단술은 높은 성공률을 보이며 척추관 협착증 환자군에 비해 추간판 탈출증 환자군에서 더 효과적인 것으로 사료된다.

• 조명전기설비학회논문지
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• 제14권6호
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• pp.69-77
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• 2000

본 논문에서는 찬우$\mid$강하법에 의한 접지저항 및 대지첸위 분표의 측캠시 전휴보조전극의 영향에 대하여 기술하였으며, 측정시 오치를 최소화하는 기법을 제안하였다. 전위강해법은 전위와 천류의 측정에 이론적인 비1깅을 두고 있으며, 측정오차는 주로 측정시 설치하는 보조전극의 위치와 자체저항에 기인한다. 피측정 접지전극의 접지저항은 전위강하법에서 전위보조전극을 피측정 접지전극과 전류보조전극이 이루는 일직선상에 위치시킬 때 61.8[%]법칙을 적용하여 측정한다. 하지만 건물이나 포장도로, 구조물 등이 산재해 있는 도심의 경우에는 현장에서 피측정 접지전극과 전류보조전극 사이에 적결한 이격거리 확보가 불가능하거냐 전류보조전극의 접지저행값이 피측정 챔 지전극에 비하여 큰 값을 가지는 경우가 있다. 파측정 접지천극과 전류보조전극을 충분히 이격시키지 않거나 전류류보조전극의 접지저항값이 피측청 접지전극의 접지저항보다 비정상척으로 큰 경우 접지저항의 측정은 오차를 수반하게 되며, 측정된 접지저항값은 오차즐 보정해 주어야 한다. 본 연구애서 측정대상 전극은 길이 2.4[m]의 봉형 접지전극으호 하였으며, 전류보조전극의 영향을 고려할 때 피측정 접지전극과 전류보조전극의 적정한 이격거리는 피측정 접치전극 길이의 5배 이상으로 하여야 전위보조전극을 사설하기가 용이하며 측정정확도의 확보에 유리하다. 또한 전류보존전극의 접지저항값이 피측정 접지전극의 접지저항값 약 36.5배 이하에서는 거의 오차를 발생시키지 않는다.

• 비파괴검사학회지
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• 제19권4호
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• pp.294-299
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• 1999

본 논문에서는 로타(rotor)의 초음파 탐상 결과의 신뢰성을 향상시키기 위하여 수직탐촉자에 대한 교정시스템을 개발하였다. 본 시스템은 신호처리부 (오실로스코프, 스펙트럼 분석기, 송/수신기), 표준시험편 프로그램으로 구성되어 있으며, 교정을 위한 신호처리는 높은 정밀도로 빠르게 수행할 수 있도록 구성되어 있다. 개발된 시스템을 이용하여 로타 검사에 사용되고 있는 초음파 탐촉자를 교정하고 있으며, 신뢰성 있는 정밀도와 감도를 얻을 수 있었다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2011년도 제40회 동계학술대회 초록집
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• pp.430-430
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• 2011

Graphene, one single atomic layer of graphite, has attracted extensive attention in various research fields since its first isolation from graphite. Application in the future electronics requires better understanding and manipulation of electronic properties of graphene supported on various solid substrates. Here, we present a study on charge doping and morphology of graphene prepared on atomically flat and highly polar mica substrates. Ultra-flat single-layer graphene was prepared by micro-exfoliation of graphite followed by deposition on cleaved mica substrates. Atomic force microscopy (AFM) revealed presence of ultra-thin water films formed in a layer-by-layer manner between graphene and mica substrates. Raman spectroscopy showed that a few angstrom-thick water films efficiently block electron transfer from graphene to mica. Hole doping in graphene caused by underlying mica substrates was also visualized by scanning Kelvin probe microscopy (SKPM).

• Animal cells and systems
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• 제3권3호
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• pp.311-317
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• 1999

Prolactin (PRL) was reported to be locally synthesized in many brain areas including the hypothalamus, thalamus (TH) and hippocampus (HIP). In the pituitary lactotrophs, PRL synthesis is dependent upon a pituitary-specific transcription factor, Pit-1. In the present study, we attempted to identify Pit-1 or Pit-1-like protein in brain areas known as the synthetic sites of PRL. Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis showed the same Pit-1 transcripts in brain areas such as the medial basal hypothalamus (MBH), preoptic area (POA), TH, and HIP with the Pit-1 transcripts in the anterior pituitary (AP). Electrophoretic mobility shift assay (EMSA) was run with nuclear protein extracts from brain tissues using a double strand oligomer probe containing a putative Pit-1 binding domain. Shifted bands were found in EMSA results with nuclear proteins from MBH, POA, TH and HIP. Specific binding of the Pit-1-like protein was further confirmed by competition with an unlabeled cold probe. Antisense Pit-1 oligodeoxynucleotide (Pit-1 ODN), which was designed to bind to the Pit-1 translation initiation site and block Pit-1 biosynthesis, was used to test Pit-1 dependent brain PRL transcription. Two nmol of Pit-1 ODN was introduced into the lateral ventricle of a 60-day old male rat brain. RNA blot hybridization and in situ hybridization indicated a decrease of PRL mRNA signals by the treatment of Pit-1 ODN. Taken together, the present study suggests that Pit-1 may play an important role in the transcriptional regulation of local PRL synthesis in the brain.