• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.13 no.3
• /
• pp.1014-1020
• /
• 2012

This paper focuses on the development of a supporting S/W tool that can minimize designer's manual operations and errors in metal mold design based on a 3D solid model. The scope in this work includes the offset surface modeling, the computation of the padding force, the generation of material table, the decision of hole position, the estimation of the size of raw material, which are the essential parts of press die and mold design in automotive industry. The proposed system has been developed as a plug-in type using Pro/E API and Visual C++ in order to put the system into the menu functions of Pro/E which is one major 3D CAD systems in the manufacturing industry.

• Applied Biological Chemistry
• /
• v.40 no.3
• /
• pp.189-195
• /
• 1997

The structural basis of Iysine specificity of Achromobacter protease I (API) was investigated by means of site-directed mutagenesis. The precursor protein in which Glu190, one of the two candidates for determining Iysine specificity, was substituted by glutamine, aspartic acid or leucine was processed autocatalytically to attaln full pretense activity with lysine specificity. The substitution of the other candidate, Asp225, for asparagine or leucine produced no mature active forms of pro-API. The precursor protein of the mutant D225E slowly matured autocatalytically. The lysylendopeptidase activity of the mature D225E was 0.25% of that of native API, and this reduced activity is mainly due to a decrease in the affinity of the enzyme for lysine. These results suggest that Asp225 plays a critical rol in restricted substrate specificity as a lysylendopeptidase. However, D225E exhibited no measurable activity for synthetic ornithine substrate. Since the hydroxyl group of Ser194 in this mutant retained essentially the same reactivity to DFP or PMSF as that in native API, it can be noted that a methylene unit longer side chain of residue 225 is not compensated by a methylene unit shorter side chain at subsite P1 in the bound substrate.

• Proceedings of the Korean Society of Marine Engineers Conference
• /
• 2005.11a
• /
• pp.258-259
• /
• 2005

• Journal of the Korean Society of Safety
• /
• v.22 no.4
• /
• pp.110-116
• /
• 2007

There have been 3E problems of energy, economy and environment since the earth has its history. Especially, as the industrial society is highly developing, human need in daily life has also changed drastically. With the introduction of 40 hour working week system, more households enjoy picnics on weekends. More gas accidents take place on Saturdays and on Sundays than any other days of week. Consequently, this study tries to find out the influence of flame caused by the explosion of butane canister on the adjacent combustibles and people by simulating relevant quantity of TNT. In addition, the damage estimation was conducted by using API regulations. If the scale of the radiation heat is known by calculating the distance of flame influence from the explosion site, the damage from the site can be easily estimated. And the accident damage was estimated by applying the influence on the adjacent structures and people into the PROBIT model. According to the pro bit analyze, the spot which is 50cm away from the flame has 97% of the damage probability by the first-degree burn, 8% of the damage probability by the second-degree burn and 4% of the death probability by the fire.