• Title/Summary/Keyword: Priming Effect

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Effect of Solid Matrix Priming on Peppers(Capsicum annuum L.) Germinability III. Germination Temperature and Duration of Dehydration (Solid Matrix Priming 처리가 고추종자의 발아에 미치는 영향 III. 발아온도와 건조기간)

  • Kim, Do-Hahn
    • Journal of agriculture & life science
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    • v.43 no.5
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    • pp.17-25
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    • 2009
  • The purpose of this study was to find out the optimum germination temperature and dehydration period at SMP processing, and applies 3 kind of seed treatment and compares yield with stand establishment of seeds of 12 pepper cultivars. It was showed that all 12 cultivars of nontreated seed does not entirely germinated in $15^{\circ}C$. It was that SMP seed germination rate 77(Yeomyeong)~100% (Deawang) high, and seeds 12 cultivars was tendency high by more than 93% at all temperatures excepting Bugang($35^{\circ}C$, 85%) and Yeomyeong($15^{\circ}C$, 77%). Dehydration condition of result were Gumsure, Dongbang Jonggagip, Hyangchon, Yeomyeong, Kwangbok, Wang by which 3 hours in $45^{\circ}C$ were proper, which 6 hours in $35^{\circ}C$ were proper Manitta, Joyang, and judged that 12 hours in $25^{\circ}C$ were proper in Bugang, Dabotap, Deawang. It was reported that water percentage of moisture content change of pepper seed by dehydration was difference a little in species, but was judged by 3-6 hours in $45^{\circ}C$. There was compared to SMP, Osmopriming, pH control by stand establishment were shown pH control of Hyangchon, Dabotap species was nontreated, osmopriming treatment. Also, Kwangbok species was tendency that stand establishment was low, and Deawang was good than other species. Yield of nontreated of yield day was tendency, and miss planted rate also appeared high for 90 day. There was difference in species, and processing of pH control and osmopriming was not shown stand establishment that is stabilized in all species. SMP processing condition could bring security of stable stand establishment in all species, and was considered that research that was under various environment condition should be proceeded conclusively.

The Effect of Pulsatile Versus Nonpulsatile Blood Flow on Viscoelasticity and Red Blood Cell Aggregation in Extracorporeal Circulation

  • Ahn, Chi Bum;Kang, Yang Jun;Kim, Myoung Gon;Yang, Sung;Lim, Choon Hak;Son, Ho Sung;Kim, Ji Sung;Lee, So Young;Son, Kuk Hui;Sun, Kyung
    • Journal of Chest Surgery
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    • v.49 no.3
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    • pp.145-150
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    • 2016
  • Background: Extracorporeal circulation (ECC) can induce alterations in blood viscoelasticity and cause red blood cell (RBC) aggregation. In this study, the authors evaluated the effects of pump flow pulsatility on blood viscoelasticity and RBC aggregation. Methods: Mongrel dogs were randomly assigned to two groups: a nonpulsatile pump group (n=6) or a pulsatile pump group (n=6). After ECC was started at a pump flow rate of 80 mL/kg/min, cardiac fibrillation was induced. Blood sampling was performed before and at 1, 2, and 3 hours after ECC commencement. To eliminate bias induced by hematocrit and plasma, all blood samples were adjusted to a hematocrit of 45% using baseline plasma. Blood viscoelasticity, plasma viscosity, hematocrit, arterial blood gas analysis, central venous $O_2$ saturation, and lactate were measured. Results: The blood viscosity and aggregation index decreased abruptly 1 hour after ECC and then remained low during ECC in both groups, but blood elasticity did not change during ECC. Blood viscosity, blood elasticity, plasma viscosity, and the aggregation index were not significantly different in the groups at any time. Hematocrit decreased abruptly 1 hour after ECC in both groups due to dilution by the priming solution used. Conclusion: After ECC, blood viscoelasticity and RBC aggregation were not different in the pulsatile and nonpulsatile groups in the adult dog model. Furthermore, pulsatile flow did not have a more harmful effect on blood viscoelasticity or RBC aggregation than nonpulsatile flow.

The Effect of Platelets on Endothelin Production in Bovine Pulmonary Artery Endothelial Cells (혈소판이 소 폐동백 내피세포의 Endothelin 생산에 미치는 효과)

  • Lee, Sang-Do;Shim, Tae-Sun;Kwon, Seog-Woon;Ryu, Jin-Sook;Lee, Jae-Dam;Lim, Chae-Man;Koh, Youn-Suck;Kim, Woo-Sung;Kim, Dong-Soon;Kim, Won-Dong
    • Tuberculosis and Respiratory Diseases
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    • v.44 no.5
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    • pp.1114-1124
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    • 1997
  • Background : Endothelin(ET) is a very potent vasoconstrictive peptide produced by endothelial cells of pulmonary artery. The endothelin level was increased in plasma of primary pulmonary hypertension and acute pulmonary thromboembolism and it was suggested that the endothelin might do a critical role in the cardiopulmonary dysfunction in these two conditions. But the exact mechanism of increase of ET has not been known. In these two conditions, platelet activation and thrombosis are the main pathophysiologic findings. So there is a possibility that the platelet might stimulate endothelin secretion from endothelial cells. Therefore, we performed this study to evaluate the role of platelet and its mediators on endothelin production in bovine pulmonary artery endothelial(BPAE) cells. Method : Bovine pulmonary artery endothelial cells, ATCC certified cell line 209, were cultured and treated with human platelets($10^6{\sim}10^8/ml$), thrombin (0.1~10u/ml), TGF-${\beta}1$(1~100uM), serotonin(1~100uM), and endotoxin(1ug/ml) in a final volume of 500ul for 18 hours. Levels of ir(immunoreactive)-ET in each conditioned medium were measured by a radioimmunoassay specific for ET. Result : The increase of ir-ET levels was platelet number and time dependent over 18 hours. When washed human platelets were added($10^8/ml$), the ir-ET levels were significantly higher than that of control(p<0.05) at 8 and 18 hours after culture. Subthreshold concentration of platelets($10^7/ml$) coincubated with endotoxin(1ug/ml) or subthreshold dose of thrombin(0.1u/ml) stimulated ir-ET secretion from BPAE cells significantly(p<0.05) compared with control. Thrombin(1ug/ml, 10ug/ml) and TGF-${\beta}1$(100pM, 1000pM) significantly increased ir-ET secretion from BP AE cells(p<0.05) compared with control, but serotoin(1~100uM) and endotoxin(1ug/ml) did not stimulate the ir-ET secretion. Conclusions : Platelets stimulate endothelin secretion from bovine pulmonary artery endothelial cells. The mechanism of increase of endothelin secretion seems to be a stimulation by platelet itself or by mediators, such as TGF-${\beta}1$, secreted from activated platelets. And, in this study, the priming effect of platelets on endothelin secretion from BPAE cells could be another possibility.

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The Effect of Ginkgo Biloba Extract on Radiosensitivity of Mouse Skin and Jejunal Crypt (Ginkgo Biloba Extract가 마우스 피부 및 공장 소낭선의 방사선감수성에 미치는 영향)

  • Shin, Kyung-Hwan;Ha, Sung-Whan
    • Radiation Oncology Journal
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    • v.16 no.2
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    • pp.107-114
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    • 1998
  • Purpose : Ginkgo biloba extract(GBE) is known to increase the peripheral blood circulation. This study was designed to evaluate the effect of GBE on the acute normal tissue radiation reaction. Materials and Methods : mice were divided into two groups, radiation alone and two doses GBE plus radiation, for both acute skin reaction and jejunal crypt assay. GBE was given i.p. one hour before irradiation with priming dose given one day earlier. Thirty to Fifty Gy for acute skin reaction and 11 to 14 Gy for jejunal crypt were irradiated to right hind leg and whole body, respectively. Results : Radiation doses($RD_{50}$) for Peak skin score of 2.0 were 44.2Gy (40.6-48.2Gy) for radiation alone and 44.4Gy(41.6-47.4Gy) for two doses GBE plus radiation, showing no effect of GBE on acute radiation skin damage. The numbers of regenerating jejunal crypts per circumference were also almost the same for each radiation dose level(p=0.57-0.94), and the mean lethal doses($D_o$) were 1.800y(1.57-2.09Gy) for radiation alone and 1.88Gy(1.65-2.18Gy) for two doses GBE plus radiation, indicating no effect of GBE on jejunal crypt cell survival after radiation. Conclusion : GBE doesn't increase acute normal tissue radiation reaction in this model system. As GBE was verified to enhance radiation effect on tumor, high therapeutic gain is expected when GBE is combined with radiation therapy.

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Effects of Low- Dose Aprotinin on Open Heart Surgery (개심술에 있어서 Low-Dose Aprotinin의 투여효과)

  • 박남희;최세영
    • Journal of Chest Surgery
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    • v.29 no.9
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    • pp.989-995
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    • 1996
  • Excessive blood loss secondary to cardiopulmonary bypass(CPB) may be encountered after open heart surgery and platelet dysfunction appears to be especially responsible for this problem. To evaluate the effect of low-dose aprotinin during hypothermic CPB on platelet aggregation, anticoagulation and clinical hemostasis,.40 patients undergoing valve replacement using hypothermic CPB procedures were randomized to give either a low dose aprotinin(2$\times$ 106 KIU in the CPB priming sol- ution, n=20) or a placebo(n=20). During postoperative 24 hours, blood and hemoglobin loss were lower in the aprotinin group (225.5 $\pm$ 121.9ml, and 11.3$\pm$2.4g) than the control group(572.2$\pm$)35.5ml and 26.3$\pm$9.8g)(P<0.01). The total blood and hemoglobin loss were lower in the aprotinin group (622.0$\pm$ 186m1 and 14.7$\pm$6.8g) than the con- trol group (102.1 $\pm$483.5ml and 39.7$\pm$ 16.4g) (P<0.01). The amonut of packed red cell needed decreased in the aprotinin group: 197.7$\pm$56.3ml vers s 651.2: 147.5ml (P<0.01). Hemoglobin concentration, platelet counts and fibrinogen checked at fixed times perioperatively did not differ between the two groups. Platelet aggregation was induced by ADP, collagen, epinephrine and ristocetin before and after CPB. Maximum platelet aggregation was significantly reduced after CPB in control group (ranging from -31 % to -58% relative to prebypass values). Significant prolongation of activated clotting time(ACT) after 5 minute and 30 minute of hypothermic CPB were observed: 955.9 $\pm$35.1 and 967.5$\pm$32.7sec versus 743.8 $\pm$ 52.1 and 731.2: 54.6sec (P<0.01). There was no complication associated with aprotinin infusion. These results demonstrate that low-dose aprotinin significantly reduces blood loss and blood requirment and provides improved postoperative hemostasis which might be related to protection of platelet aggregation capacity.

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The effect of self-regulatory focus on loss aversion when gain and loss between possession and valence compete (소유와 유인가의 득과 실이 경쟁할 때 자기조절초점이 손실회피에 미치는 효과)

  • Lee, Byung-Kwan;Lee, Guk-Hee
    • Science of Emotion and Sensibility
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    • v.17 no.4
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    • pp.101-108
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    • 2014
  • The study of loss aversion has progressed. Specifically, Brenner et al. (2007) distinguished between possession loss aversion (PLA) and valence loss aversion (VLA) and explained PLA is the loss of cash or spot goods (money, apartment) and that VLA is the loss of tentative value (opportunity of oversea study, coupon). Also, they explored how the tendency of loss aversion was different between when possession loss was salience and when valence loss was outstanding. But, in our everyday life, it is more common that people face some economic situation where the gain and loss between possession and valence are competing. And, in this situation, they usually choose a better option based on their ordinary mind-set or self-focus rather than on which option has more benefit. Therefore, present research is that whether the tendency of loss aversion is different based on one's ordinary self-focus. Concretely, after priming promotion focus vs. prevention focus, participants should decide whether present occupation changes or not when gain and loss between possession and valence compete (income increase but oversea study chance decrease or income decrease but oversea study chance increase). As a result, there are more rate of changing job in promotion focusing condition than prevention focusing condition based on positive change irrespective of possession or valence. This result can have implication for marketing, consumer and advertisement psychology.

Effect of Seeds Treatment on Germinablity of Tetragonia Tetragonides Seeds (번행초의 대량번식을 위한 종자처리가 발아력에 미치는 영향)

  • Kang, Jum-Soon;Park, Eun-Ji;Kim, So-Hee;Heo, You;Park, Young-Hoon;Choi, Young-Whan;Son, Beung-Gu;Lim, Woo-Taik;Suh, Jeong-Min
    • Journal of Environmental Science International
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    • v.23 no.5
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    • pp.771-780
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    • 2014
  • Tetragonia tetragonides is a medicinal plant native to ocean sand soil of southern provinces and has significant effects on the prevention and curing of gastroenteric disorders. Despite of its popularity, supply of the plant has never met the level of demand because of the absence of an adequate culturing method. The present study, thereby, was conducted for classifying the plants with geographically different characteristics, studying growth habits, developing a new culturing method and establishing a large scale propagation system of selected superior individual plants. The study was also aimed for revealing optimum conditions for seed treatment, fertilization, and efficient culturing system and thereby, for utilizing the plant as a new income source for rural communities. The seed was elongated with size of 2.6 mm (width) ${\times}$ 1.8 mm (length). No difference in seed size was observed depending on different inhabitate. Each flower produced about 4.5~4.8 seeds. Germination rate was high for seeds matured for 40 days after fertilization, but deceased to 50% for seeds matured only for 20 or 30 days. Seed dormancy lasted 6 months and seed storage at humid $5^{\circ}C$ facilitated germination. Mechanical obstruct of seed germination was due to seed coat and removal of seed coat enhanced the germination rate. Optimum temp. for seed storage was $5^{\circ}C$, and high germination rate was maintained for 350 days. However, for stratification condition or at room temperature, germination was significantly reduced as storage time increased Optimum treatment of plant growth regulators was soaking in $GA_3$ 250 mg/L for 1 hr. The priming treatment with 50 mM $Ca(NO_3)_2$ at $20^{\circ}C$ for two days improved the seed germination with 10% compared to non-treated control. The treatment of 20% NaOCl for 3 hr. improved the seed germination rate up to 10% and 1 day ahead.

Improving Germination and Seedling Growth of Rigitaeda Pine Tree by Coating and Prime Treatment (리기테다 소나무 종자(種子)의 피복(被覆)과 전처리(前處理)에 의한 발아(發芽) 및 유묘(幼苗) 생장(生長) 촉진(促進))

  • Koh, D.S.;Hur, S.N.;Seo, B.S.
    • Journal of Korean Society of Forest Science
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    • v.83 no.4
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    • pp.505-511
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    • 1994
  • Seeds of Rigitaeda pine tree(Pinus rigida${\times}$P. taeda) was primed with polyethylene glycol(PEG-6000) under different PEG concentrations, treatment period, and temperatures to test uniformity of germination. Coated seeds and PEG treated seeds were sown to compare germination, emergence, establishment, and seedling growth. The results obtained are summarized as follows : 1. As the concentration of PEG increased, as total germination percentage of pine tree was increased. Total germination percentage of pine tree seeds primed with PEG at 15 or $20^{\circ}C$ was better than the seeds treated at $10^{\circ}C$ compared to untreated seeds. 2. Germination rate was improved as primed period long and level of PEG concentration high. 3. Maximum germination rate was high with long PEG treatment period, and markedly improved when the seeds were primed at $20^{\circ}C$ 4. Priming with PEG at $20^{\circ}C$ for 12 days reduced time taken for germination. 5. Osmoconditioned seeds accelerated germination under drought condition with injurious effect of coated seeds by some chemicals. 6. There were much differences in establishment and dry matter production between drill and oversowing method, and primed seeds showed better performances than the coated seeds. Coating effects to seeds were better under drought soil condition rather than moderate moisture condition.

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Accelerated Germination of Orchardgrass(Dactylis glomerata) Seed Under Water Stress by Seed Pre-treatments (수분 장애시 종자 전처리가 오차드그라스의 발아에 미치는 영향)

  • Baek, Seong-Beom;Kim, Geon-Yeop;Kim, Heung-Bae
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.13 no.1
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    • pp.31-37
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    • 1993
  • It is important to improve the germination of grass seeds because they are poor germination under water stress. This experiment was conducted to investigate the response of seeds pre-treated individually with Polyethylene glycol 6000 (PEG) and growth regulators(GA$_3$, Kinetin, NAA) on the germination characteristics when two levels of osmotic potential (0, -5 bar) were put to seeds of Orchardgrass. In the untreated seeds, the total germination rate was decreased under water stress, and the mean germination time was delayed. Priming of Orchardgrass seeds using PEG or GA$_3$ resulted in improved germination under water stress(-5 bar), whereas the opposite was true of kinetin or NAA. The response of seeds primed in solutions of either -15 bar PEG or 100 ppm GA$_3$ was particulary marked compared with the untreated seeds. It is suggested that PEG and GA$_3$ may have positive regulatory effects in triggering the system for water stress alleviation. But the inhibitory effect of the water stress was not completely removed by allowing the seeds to pre-treat in solution of PEG or GA$_3$.

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Hemispheric Asymmetry in Processing Semantic Relationship Shown in Normals and Aphasic (형태소 공유 어휘의 심성 어휘집 표상 양식)

  • Jung, Jae-Bum;Lee, Hong-Jae;Moon, Young-Sun;Kim, Dong-Hyu;Pyun, Sung-Bum;Nam, Ki-Chun
    • Annual Conference on Human and Language Technology
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    • 1999.10e
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    • pp.359-367
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    • 1999
  • 형태소를 공유하고 있는 어휘가 심성 어휘집(mental lexicon)에 어떻게 저장되어 있고 어떻게 어휘 접근되는지에 관하여 여러 설명이 제기되었다 첫 번째 가설은 형태소 공유 어휘는 심성 어휘집에 모두 같은 어근 혹은 어간을 중심으로 저장되어 있다는 것이다. 두 번째 가설은 어간이나 어근으로의 분석을 통해 활용된 단어를 이해하는 것이 아니라 일단 활용된 형태의 어휘를 심성 어휘집에서 찾고, 만일 해당되는 것이 발견되면, 그 활용된 어절의 이해가 끝나게 되고, 만일에 해당되는 것이 심성 어휘집에 존재하지 않는 경우에만 부수적인 과정으로 구성 형태소로의 분석이 이루어진다는 것이다. 세 번째 가설은 어휘의 품사, 어휘의 빈도, 형태소 활용의 규칙성 등에 따라 구성 형태소로의 분석을 통해 활용된 단어를 이해하거나 아니면 활용된 어휘의 직접적인 접근을 통해 활용된 단어를 이해한다는 것이다. 본 연구에서는 이 세 종류의 가설 중에 어느 가설이 옳은 것인지를 조사하기 위해, "먹은" 흑은 "쥐어"와 같은 한국어 어절을 이용하여 형태소 표상 양식과 이해 과정을 다루었다. 본 연구의 목적을 위해 점화 어휘 판단 과제(primed-lexical decision task)를 사용하였다. 실험 1은 "먹은"처럼 동사 "먹다"로도 해석이 가능하고 명사 "먹"으로도 가능한 중의적 어절을 점화 문자열로 제시하고 이 문자열이 두 의미와 관련된 목표 단어 재인에 어떤 영향을 끼치는지를 조사하였다. 만일에 "먹"이라는 어근 혹은 어간으로의 분석을 통해 이 어절을 이해한다면 두 종류의 의미와 관련된 조건 모두에서 촉진적 점화 효과(facilitatory priming effect)가 나타날 것이고, 어절 전체로의 어휘 접근 과정이 일어난다면 사용빈도에서 높은 동사 뜻과 관련된 조건에서만 촉진적 점화 효과가 나타날 것이다. 실험 1의 결과는 두 종류의 의미가 모두 활성화되는 것을 보여 주었다. 즉, "먹은"과 간은 어절 이해는 구성 형태소로의 분석과 구성 형태소 어휘 접근을 통해 어절 이해가 이루어진다는 가설을 지지하고 있다. 실험 2에서는 실험 1과 다르게 한 뜻으로만 안일 수밖에 없는 "쥐어"와 같은 어절을 사용하여 이런 경우에도(즉, 어절의 문맥이 특정 뜻으로 한정하는 경우) 구성 형태소로의 분석 과정이 일어나는지를 조사하였다. 실험 2의 결과는 실험 1의 결과와는 다르게 어간의 한가지 의미와 관련된 조건만 촉진적 점화 효과가 나타나는 것을 보여주었다. 특히, 실험 2에서 SOA가 1000msec일 경우, 두 의미의 활성화가 나타나는 것을 보여주었는데, 이 같은 결과는 어절 문맥이 특정한 의미로 한정시킬 경우는 심성어휘집에 활용형태로 들어있다는 것이다. 또한 명칭성 실어증 환자의 경우에는 즉시적 점화과제에서는 일반인과 같은 형태소 처리과정을 보였으나, 그이후의 처리과정이 일반인과 다른 형태를 보였다. 실험 1과 실험 2의 결과는 한국어 어절 분석이 구문분석 또는 활용형태를 통해 어휘 접근되는 가설을 지지하고 있다. 또 명칭성 실어증 환자의 경우에는 지연된 점화과제에서 형태소 처리가 일반인과 다르다는 것이 밝혀졌다. 이 결과가 옳다면 한국의 심성 어휘집은 어절 문맥에 따라서 어간이나 어근 또는 활용형 그 자체로 이루어져 있을 것이다.

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