• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.26 no.3
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• pp.245-253
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• 2000

The development and progression of oral cancer is associated with an accumulation of multiple genetic alterations through the multistep processes. Comparative genomic hybridization(CGH), newly developed cytogenetic and molecular biologic technique, has been widely accepted as a useful method to allow the detection of genetic imbalance in solid tumors and the screening for chromosome sites frequently affected by gains or losses in DNA copy number. The authors examined 19 primary oral squamous cell carcinomas using CGH to identify altered chromosome regions that might contain novel oncogenes and tumor suppressor genes. Interrelationship between these genetic aberrations detected and major oncogenes and tumor suppressor genes previously recognized in carcinogenesis of oral cancers was studied. 1. Changes in DNA copy number were detected in 14 of 19 oral cancers (78.9%, mean: 5.58, range: $3{\sim}13$). High level amplification was present in 4 cases at 9p23, $12p21.1{\sim}q13.1$, 3q and $8q24{\sim}24.3$. Fourteen cases(78.9%, mean: 3.00, range: $1{\sim}8$) showed gains of DNA copy number and 12 cases(70.5%, mean: 2.58, range: $1{\sim}9$) revealed losses of DNA copy number. 2. The most common gains were detected on 3q(52.6%), 5p(21.0%), 8q(21.0%), 9p(21.0%), and 11q(21.0%). The losses of DNA copy number were frequently occurred at 9p(36.8%), 17q(36.8%), 13q(26.3%), 4p(21.0%) and 9p(21.0%). 3. The minimal common regions of gains were repeatedly observed at $3q24{\sim}26.7$, $3q27{\sim}29$, $1q22{\sim}31$, $5p12{\sim}13.3$, $8q23{\sim}24$, and 11q13.1-13.3. The minimal common regions of losses were detected at $9q11{\sim}21.3$, 17p31, $13q22{\sim}34$, and 14p16. 4. In comparison of CGH results with tumor stages, the lower stage group showed more frequent gain at 3q, 5q, 9p, and 14q, whereas gains at 1q($1q22{\sim}31$) and 11q($11q13.1{\sim}13.3$) were mainly detected in higher stage group. The loss at $13q22{\sim}34$ was exclusively detected in higher stage. The results indicate that the most frequent genetic alterations in the development of oral cancers were gains at $3q24{\sim}26.3$, $1q22{\sim}31$, and $5p12{\sim}13.3$ and losses at $9q11{\sim}21.3$, 17p31, and 13q. It is suggested that genetic alterations manifested as gains at $3q24{\sim}26.3$, $3q27{\sim}29$, $5p12{\sim}13.3$ and 5p are associated with the early progression of oral cancer. Gains at $1q22{\sim}31$ and $11q13.1{\sim}13.3$ and loss at 13q22-34 could be involved in the late progression of oral cancers.

• Proceedings of the Korean Information Science Society Conference
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• 2005.11a
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• pp.334-336
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• 2005

이동적응망(MANET: Mobile Ad-hoc Network)은 네트워크 하부 구조를 필요로 하지 않은 무선 단말들로 구성된 네트워크이다. 이러한 특성은 네트워크 단절 가능성을 높게 하기 때문에 이동단말들의 데이터 액세스률이 낮아지게 된다는 문제점을 갖는다. 이를 해결하기 위하여 이동 노드들은 데이터의 중복사본을 갖는다. 이동 노드가 갖는 중복사본은 데이터 일관성을 유지하기 위하여 별도의 중복관리 기법이 필요하다. 하지만 MANET을 구성하는 이동 노드들은 일반적으로 제한된 전력을 가지고 있고 단절될 가능성이 높기 때문에 중복 사본의 일관성 보장은 어려운 문제로 지적되고 있다. 기존에 제안된 MANET에서의 데이터 중복관리 기법은 데이터 액세스 빈도수를 계산하여 액세스률을 높이는 방법에 주안점을 두고 있고 갱신 데이터의 일관성 보장은 그 어려움 때문에 주로 판독 연산만 고려하였다. 갱신 트랜잭션을 지원하는 경우 대부분 높은 통신비용을 이유로 데이터 일관성을 보장하지 않는다. 또한 이동 노드가 다수의 서버를 통해서 갱신 연산을 실행하기 때문에 통신 오버헤드로 인하여 전력소모가 크다. 본 논문에서는 주사본 노드를 통하여 갱신을 가능하게 함으로써 데이터 일관성을 유지할 수 있는 데이터 중복 기법을 제안한다. 제안된 기법은 이동 노드들의 에너지 특성을 고려하여 더 않은 에너지를 가진 노드에게 갱신 전파 및 일관성 유지를 의뢰함으로써 상대적으로 낮은 에너지를 갖는 이동 노드의 에너지 효율을 고려하였다.

• Journal of ILASS-Korea
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• v.17 no.4
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• pp.185-191
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• 2012

Particulate matters (PM) that is generated by most diesel engine is regulated by the mass concentration measured by the conventional method it had been. Recently, Europe PMP (Particle Measurement Program) decided to start the regulation of vehicle's nano-sized particle number (PN) from the year of 2011 because of nano-particle's higher degree of harm to the human body. So firstly, the standard level of PN emission is introduced in the Euro 5/6 emissions regulation with a limit of $6{\times}10^{11}$ per km for light duty vehicle. Also KPMP(Korea Particle Measurement Program) was organized to copy quickly international technical trend. In this paper, it was investigated the nano-sized PN measurement characteristics for different particle generators between spray type and soot type. And the difference ratio between particle generators, the characteristic of PN concentration, counting efficiency and linearity was analyzed. Then, we make conclusions as followed. When particle diameter is increased, counting efficiency of two generators is decreased. Also Secondary calibration method is more higher 3% than Primary calibration method. Finally, SOF which is included in soot particles is not totally removed so it have great influence on test result of counting efficiency and linearity.

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.103-106
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• 2002

Direct PCR from intact fungal cells is not readily suitable to all fungi mainly because of difficulties in rupturing the cell walls. Microwave irradiation has been proven to be useful in fungal DNA extraction protocol. Here we describe a fast template preparation method for PCR amplification from Aspefillus nidulans conidiospores using microwave irradiation. We optimized the duration far microwave irradiation, and the amount of template DNA for PCR. Amplification from samples prepared in this manner was so efficient that we could get PCR products with size enough to identify transformants. We believe that this is a time-saving procedure for screening true transformants of A. nidulans.

• The Korean Journal of Archival Studies
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• no.29
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• pp.129-173
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• 2011

Standards-curriculum based archival contents for education is the best effective teaching and learning units for historical thinking abilities. This paper purposes a developing procedures of an archival contents for education that is theoretical instructions of developing an archival contents for education by the National Archives of Korea. This paper can be used of the theoretical bases for the National Archives of Korea by proposing the methodology of development of an archival contents for education. The developing procedures of an archival contents for education is the same with the procedures of developing an e-learning contents that has planning, analyzing, designing, developing and assessing steps but it is characterized by an archival contents for education that is curriculum standards analysis, collection analysis, and detailed design for structured formats in effective-accomplishments for teaching-learning objectives. I propose the procedures for determining teaching-learning subjects that enable the development of an archival contents for education by curriculum standards analysis. I also propose the procedures for deriving the key words from the teaching-learning subjects. Collection analysis methods analyze key records that correspond to the learning subjects according to the selection criteria of primary sources. In the steps of designing, titles of contents and contents structures have to be determined and storyboards based on flowchart of learning have to be made of according to the results of analyses. In the steps of developing contents, making a copy of primary sources like a original is the key points. And also in the steps of assessment, products of teaching-learning contents to effectively achieve the teaching-learning objectives have to be estimated by the appraisal board. Finally I propose that user's survey research after the services have to be reflected on contents updates and new developments of contents.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.43 no.5 s.311
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• pp.30-36
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• 2006

In this paper, a new type of written image segmentation based on relative comparison of region areas is proposed. The original image is composed of two distinctive regions; information and background. Compared with this binary original image, the observed one is the gray scale which is represented with complex regions with speckles and noise due to degradation or contamination. For applying threshold or statistical approach, there occurs the region-deformation problem in the process of binarization. At first step, the efficient iterated conditional mode (ICM) which takes the lozenge type block is used for regions formation into the binary image. Secondly the information region is estimated through selecting action and restored its primary state. Not only decision of the attachment to a region but also the calculation of the magnitude of its area are carried on at each current pixel iteratively. All region areas are sorted into a set and selected through the decision parameter which is obtained statistically. Our experiments show that these approaches are effective on ink-rubbed copy image (拓本 'Takbon') and efficient at shape restoration. Experiments on gray scale image show promising shape extraction results, comparing with the threshold-segmentation and conventional ICM method.

• The Transactions of the Korea Information Processing Society
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• v.7 no.8
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• pp.2310-2326
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• 2000

Client-server DBMS based on a data-shipping model can exploit e1ient resources effectively by allowing inter-transaction caching. However, inter-transaction caching raises the need of transactional cache consistency maintenancetTCCM protocol. since each client is able to cache a portion of the database dynamically. Deferred locking(DL) is a new detection-based TCCM scheme designed on the basis of a primary copy locking algorithm. In DL, a number of lock ,ujuests and a data shipping request are combined into a single message packet to minimize the communication overhead required for consistency checking. Lsing a simulation model. the performance of the prolxlsed scheme is compared with those of two representative detection based schemes, the adaptive optimistic concurrency control and the caching two-phase locking. The performance results indicate that DL improves the overall system throughput with a reasonable transaction abort ratio over other detection - based schemes.

• The Journal of Information Technology
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• v.10 no.2
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• pp.37-45
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• 2007

The multiple-ability which the structure and the physical properties which the carbon or scull tube are unique show the applicability is superior in the plane indication element which is an indispensability of information communications apparatus, the stubbornness memory element, 2nd change of air and the rough copy dosage [khay] plaque seater, the hydrogen store material and the chemical sensor back and it has the possibility which will pass over the limit which the element of existing has. from the present paper it compared in the steel and only 10 the boat it did and it analyzed against an energy storage space voluntary application and developmental apply the carbon or scull tube trend in order about under researching the effective energy storage element it could be appeared, the technique of the strong carbon nano tube. 1. The hazard which embodies the energy storage element which uses the carbon or scull tube it follows in the function which stands and CNT of the structure which is various is necessary. 2. CNT fabrications of each one must precede possible not only must be each Cabinet conference circumstances quality gain and loss. 3. The structural control of syntheses, length controls, diameter controls and the metal - CNT junction control backs of quality CNT must precede. Applies the hereafter carbon or the scull tube in the various element with the primary preceding base technique for the structural plan technique of the carbon or scull tube to be certainly established, it does, secondarily the various element functional control technique which uses the carbon or scull tube is researched and will do.

• Molecular & Cellular Toxicology
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• v.5 no.3
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• pp.216-221
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• 2009

Bisphenol A (BPA) is commonly used in the production of pharmaceutical, industrial, and housing epoxy, as well as polycarbonate plastics. Owing to its extensive use, BPA can contaminate the environment either directly or through derivatives of these products. BPA has been classified as an endocrine disruptor chemicals (EDCs), and the primary toxicity of these EDCs in males involves the induction of reproductive system abnormality. First, in order to evaluate the direct effects on the Y chromosome associated with reproduction, we evaluated Y chromosome abnormalities using a Y chromosome microdeletion detection kit. However, we detected no Yq abnormality as the result of BPA exposure. Secondly, we performed high-density oligonucleotide array-based comparative genome hybridization (CGH) to assess genomic alteration as a component of our toxicity assessment. The results of our data analysis revealed some changes in copy number. Seven observed features were gains or losses in chromosomal DNA (P-value<1.0e-5, average log2 ratio>0.2). Interestingly, 21 probes of chr7:7312289-10272836 (qA1-qA2 in cytoband) were a commonly observed amplification (P-value 3.69e-10). Another region, chr14:4551029-10397399, was also commonly amplified (P-value 2.93e-12, average of log2 ratios in segment>0.3786). These regions include many genes associated with pheromone response, transcription, and signal transduction using ArrayToKegg software. These results help us to understand the molecular mechanisms underlying the reproductive effects induced by BPA.

• Journal of Plant Biotechnology
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• v.35 no.3
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• pp.177-183
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• 2008

Ac/Ds mutant lines of this study were transgenic rice plants, each of which harbored the maize transposable element Ds together with a GUS coding sequence under the control of a promoterless(Ds-GUS). We selected the mutants that were GUS expressed lines, because the GUS positive lines will be useful for identifying gene function in rice. One of these mutants was identified knock-out at Oszinc626(NP_001049991) gene, encoding a RING-H2 zinc-finger protein, by Ds insertion. In this mutant, while primary root development is normal, secondary root development from lateral root was very poor and seed development was incomplete compare with normal plant. RING zinc-finger proteins play important roles in the regulation of development in a variety of organisms. In the plant kingdom, a few genes encoding RING zinc-finger proteins have been documented with visible effects on plant growth and development. The consensus of the RING-H2(C3-H2-C3 type) domain for this group of protein is $Cys-X_2-Cys-X_{28}-Cys-X-His-X_2-His-X_2-Cys-X_{14}-Cys-X_2-Cys$. Oszinc626 encodes a predicted protein product of 445 amino acids residues with a molecular mass of 49 kDa, with a RING-zinc-finger motif located at the extreme end of the C-terminus. RT-PCR analysis indicated that the expression of Oszinc626 gene was induced by IAA, cold, dehydration, high-salinity and abscisic acid, but not by 2,4-D, and the transcription of Oszinc626 gene accumulated primarily in rice immature seeds, root meristem and shoots. The gene accumulation patterns were corresponded with GUS expression.