• Mass Spectrometry Letters
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• v.15 no.3
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• pp.121-140
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• 2024

Proteoform diversity is greatly increased by glycosylation, the primary post-translational modification of proteins. Glycans, also known as oligosaccharides, are molecules that are essential to almost all living things. They can affect protein folding and functionality, modulate cell-cell interactions, and support the proliferation of numerous diseases when they are found on cell surfaces or bound to proteins. A thorough understanding of their fundamental structure is necessary to gain insight into their characteristics and functions. But a major obstacle is the structural intricacy of glycans by design. The stereochemistry and regiochemistry of carbohydrates vary and are frequently branched. Because of its superior sensitivity and the abundance of fragmentation information it can provide, mass spectrometry is now the method of choice for glycan and glycopeptide analysis. Differentiating between the structures of isomeric and isobaric glycopeptides, however, presents a difficulty for MS-based characterization. Ion mobility plus mass spectrometry (IM-MS) has become a very promising new method for glycan research in recent years. Recent developments in the growing discipline of glycosylation analysis utilizing IM-MS are outlined in this review, with a focus on the MS methodology and its ability to resolve isomeric glycans.

• Physical Therapy Korea
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• v.14 no.4
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• pp.66-74
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• 2007

The aim of the present study was to investigate age-related differences in stepping behavior in response to sensory perturbations of postural balance. The participants for this study were 2 healthy elderly adults (mean age=76.0) and 2 younger adults (mean age=25.5). Subjects were asked to step over a 10 cm high obstacle at self-paced speed with the right limb to land on the primary target (normal step length) that is 10 cm in diameter. However, if, during movement, the light was illuminated, then the subject had to step on the secondary target (long step length). It was planned that the onset of the light would be prior to peak Fx of swing limb, between swing peak Fx and swing toe-off, and after swing toe-off. In the younger adults these secondary visual cues were provided at mean times of 240 ms (standard deviation (SD)=11), 402 ms (SD=13), and 476 ms (SD=88) following the movement onset. Corresponding mean times for the healthy elderly were 150 ms (SD=67), 352 ms (SD=39), and 562 ms (SD=115). Results showed great changes in both group and visual cue condition in Fx ground reaction forces and temporal events following the swing toe-off. Swing limb acceleration force (Fx) and stance peak Fx1 was much greater in the young adults compared to the older adults. Both young and older adults increased stance peak Fx2 in the visual cue condition compared to normal stepping. There was no difference in stance peak Fx2 between the visual cue conditions in both groups. Similarly, the time to stance peak Fx2 was much longer for the visual cue condition than for the normal stepping. It was not different between the visual cue conditions in the young adults, but in the elderly mid and late cue was much greater than early cue. In addition, time to stance peak Fx2 and swing and stance time were much longer in the older adults compared to the young adults for the visual cue conditions. These results suggest that unlike young adults, elderly adults did not flexibly modify their responses to unexpected changes in step length while stepping over obstacles.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.47 no.2
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• pp.147-154
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• 2024

This study focuses on the path planning algorithm for large-scale autonomous delivery using drones and robots in urban environments. When generating delivery routes in urban environments, it is essential that avoid obstacles such as buildings, parking lots, or any other obstacles that could cause property damage. A commonly used method for obstacle avoidance is the grid-based A* algorithm. However, in large-scale urban environments, it is not feasible to set the resolution of the grid too high. If the grid cells are not sufficiently small during path planning, inefficient paths might be generated when avoiding obstacles, and smaller obstacles might be overlooked. To solve these issues, this study proposes a method that initially creates a low-resolution wide-area grid and then progressively reduces the grid cell size in areas containing registered obstacles to maintain real-time efficiency in generating paths. To implement this, obstacles in the operational area must first be registered on the map. When obstacle information is updated, the cells containing obstacles are processed as a primary subdivision, and cells closer to the obstacles are processed as a secondary subdivision. This approach is validated in a simulation environment and compared with the previous research according to the computing time and the path distance.

• Journal of Korean Neurosurgical Society
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• v.30 no.sup2
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• pp.228-234
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• 2001

Objective : The goal of this study was to identify variables that were predictive of recurrence in primary intracranial ependymomas. Methods : We analyzed variables affecting recurrence in 30 patients with primary intracranial ependymomas. Age, location, CSF cytology, seeding on neuroimaging study, tumor grade, extent of surgery, use of chemotherapy, chemotherapy regimen, use of radiotherapy, and radiotherapy field were entered to test their impacts on recurrence. Results : Follow-up ranged from 2 to 110 months. Tumors were recurred at the primary tumor site only in 13 patients (43.3%). The overall average recurrence free period was 55 months, with overall recurrence free rates at 3 and 6 years of 61.0% and 20.9%, respectively. Extent of surgery was the strongest variable affecting recurrence. The median recurrence free period and 3-year recurrence free rate were 72 months and 78.4% for patients having complete excision and 33 months and 0% for those having incomplete excision(p=0.05). Other prognostic variables like age, location, tumor grade, use of chemotherapy, and use of radiotherapy did not affect recurrence(p=0.2848, 0.7899, 0.1714, 0.2157, 0.7076, respectively). Conclusions : Intracranial ependymomas have a propensity to recur after treatment, and recurrence at the primary site is still the main obstacle to cure. Among various variables, only extent of resection had the strongest impact on recurrence. Additional studies may still be needed to precisely define the prognostic variables on recurrence in intracranial ependymomas.

• International Journal of Fuzzy Logic and Intelligent Systems
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• v.15 no.4
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• pp.305-314
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• 2015

The work presented in this paper deals with a navigation problem for multiple mobile robot system in unknown indoor environments. The environment is completely unknown for all the robots and the surrounding information should be detected by the proximity sensors installed on the robots' bodies. In order to guide all the robots to move along collision-free paths and reach the goal positions, a navigation method based on the combination of a set of primary strategies has been developed. The indoor environments usually contain convex and concave obstacles. In this work, a danger judgment strategy in accordance with the sensors' data is used for avoiding small convex obstacles or moving objects which include both dynamic obstacles and other robots. For big convex obstacles or concave ones, a wall following strategy is designed for dealing with these special situations. In this paper, a state memorizing strategy is also proposed for the "infinite repetition" or "dead cycle" situations. Finally, when there is no collision risk, the robots will be guided towards the targets according to a target positioning strategy. Most of these strategies are achieved by the means of fuzzy logic controllers and uniformly applied for every robot. The simulation experiments verified that the proposed method has a positive effectiveness for the navigation problem.

• Journal of the Optical Society of Korea
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• v.19 no.4
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• pp.334-339
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• 2015

Terabit-per-second (Tb/s) transmission capacity for the next generation of long-haul communication networks can be achieved using multicarrier optical super-channel technology. In an elastic orthogonal frequency division multiplexing (OFDM) super-channel transmission system, demultiplexing a portion of an entire spectrum in the form of a subband with minimum power is critically required. A major obstacle to achieving this goal is the analog-to-digital converter (ADC), which is power-hungry and extremely expensive. Without a proper ADC that can work with low power, it is unrealistic to design a 100G coherent receiver suitable for a commercially deployable optical network. Discrete Fourier transform (DFT) is often seen as a primary technique for understanding partial demultiplexing, which can be attained either optically or electronically. If fairly comparable performance can be achieved with an all-optical DFT circuit, then a solution independent of data rate and modulation format can be obtained. In this paper, we investigate two distinct OFDM super-channel receiver models, based on electronic and all-optical DFT-technologies, for partial carrier demultiplexing in a multi-Tb/s transmission system. The performance comparison of the receivers is discussed in terms of bit-error-rate (BER) performance.

• Journal of the Korean Institute of Educational Facilities
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• v.7 no.3
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• pp.23-35
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• 2000

Today the supply of school sports halls for multi-functional use is absolutely insufficient in school facilities of Korea. For economical reasons and efficiency of use, a joint utilization of these halls by schools, sports clubs and other users should be strived for. This will lead, as a result, to a minimization overall in the demand for halls. Above all to satisfy this demand, the regulations of sports facilities suitable to the students' standards of play and physical conditions must be preceded in Korea. And a study on the architectural planning of this hall size for primary, middle and high school is needed. This study aims at establishing the range of optimum size of school sports hall in Korea by surveying and analyzing the regulations of sports facilities about clear height of hall, obstacle-free zone and court area in Japan, England, Germany and U.S.A. Therefore, this study will offer valuable basic data in planning the school sports hall.

• Korean Parent-Child Health Journal
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• v.3 no.2
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• pp.67-80
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• 2000

This study was described as midwife's role and obstacle of midwife's role expansion. Midwife as primary medical personal who practices for a mother and infant health care and promotion of mother infant interaction. As the trend of increasing natural childbirth, midwifery has to provide childbearing care those who want delivery in a midwifery center. This study conducted to survey for 44 midwives who work at the midwifery center. The results of the study as fellows. 1. Most of the midwives role was care of pregnancy, delivery, postpartum women and babies. Another role was conducted educational classes childbirth, breast feeding, contraception and sexual education. 2. Some midwives role perform breech, vaccum delivery, episiotomy and suture, pitocin induction and augmentation, ultrasonogram, giving medication, anesthesia, collecting specimen from Pap smear and vaginal discharge. Midwife perform these roles without medical law support. 3. Most of the obstacles of the midwife role was the medical law limitation. Midwives want revise medical law to perform simple treatment for childbearing women and babies. 4. Half of the midwives refer cases to medical doctor in case of complication of women and newborns. 5. Current frequency of home birth rate is slightly higher than before and me cases like to have delivery under water. Finally, midwife and midwifery have to prepare to meet childbearing woman, baby and family's need. For activation and expansion of midwife's role, every midwife has to be aware of medical law accurately and they must know what practice they can do and what practice they can not do.

• Journal of Genetic Medicine
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• v.12 no.2
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• pp.72-78
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• 2015

Recently, noninvasive prenatal test (NIPT) has been adopted as a primary screening tool for fetal chromosomal aneuploidy. The principle of NIPT lies in isolating the fetal fraction of cell-free DNA in maternal plasma and analyzing it with bioinformatic tools to measure the amount of gene from the target chromosome, such as chromosomes 21, 18, and 13. NIPT will contribute to decreasing the need for unnecessary invasive procedures, including amniocentesis and chorionic villi sampling, for confirming fetal aneuploidy because of its higher positive predictive value than that of the conventional prenatal screening method. However, its greater cost than that of the current antenatal screening protocol may be an obstacle to the adoption of this innovative technique in clinical practice. Digital polymerase chain reaction (dPCR) is a novel approach for detecting and quantifying nucleic acid. dPCR provides real-time diagnostic advantages with higher sensitivity, accuracy, and absolute quantification than conventional quantitative PCR. Since the groundbreaking discovery that fetal cell-free nucleic acid exists in maternal plasma was reported, dPCR has been used for the quantification of fetal DNA and for screening for fetal aneuploidy. It has been suggested that dPCR will decrease the cost by targeting specific sequences in the target chromosome, and dPCR-based noninvasive testing will facilitate progress toward the implementation of a noninvasive approach for screening for trisomy 21, 18, and 13. In this review, we highlight the principle of dPCR and discuss its future implications in clinical practice.

• The Journal of the Acoustical Society of Korea
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• v.23 no.2E
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• pp.56-67
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• 2004

Despite abundant research outcomes of blind source separation (BSS) in many types of simulated environments, their performances are still not satisfactory to be applied to the real environments. The major obstacle may seem the finite filter length of the assumed mixing model and the nonlinear sensor noises. This paper presents a two-step speech enhancement method with multiple microphone inputs. The first step performs a frequency-domain BSS algorithm to produce multiple outputs without any prior knowledge of the mixed source signals. The second step further removes the remaining cross-channel interference by a spectral cancellation approach using a probabilistic source absence/presence detection technique. The desired primary source is detected every frame of the signal, and the secondary source is estimated in the power spectral domain using the other BSS output as a reference interfering source. Then the estimated secondary source is subtracted to reduce the cross-channel interference. Our experimental results show good separation enhancement performances on the real recordings of speech and music signals compared to the conventional BSS methods.