• Title/Summary/Keyword: Primary mineral

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Synthesis lit Alcohol-oxidase in Hansenula polymoypha on Various Carbon Sources (여러가지 탄소원에 의한 Hansenula polymoypha의 Alcohol-oxidase합성)

  • 이명숙;장동석;최위경
    • Microbiology and Biotechnology Letters
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    • v.17 no.5
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    • pp.461-467
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    • 1989
  • The regulation of the synthesis of alcohol-oxidase (E.C.1.1.3.13.) was investigated in the methanol-utilizing yeasts during growth on different carbon sources. For this experiment, Hansenula polymoypha CBS 4132, Hansenula polymoypha CBM 11 and Hansenula polymoypha Cooney were cultured in mineral salt medium by changing its carbon sources. The production of alcohol-oxidase was varied by the carbon sources. For exmaple, alcohol-oxidase was undetectable: in all strains submitted to the test in the medium with glucose, but its production was rapidly increased when the carbon source was changed from glucose to methanol after 30 hrs of incubation. Moreover, this enzyme was not synthesized during growth on the primary aliphatic alcohols alone (ethanol, propanol, butanol or pentanol) or on the mixed substrates (0.5% methanol + 0.5% primary aliphatic alcohols). When cells were grown on the various carbon sources (glucose, xylose, lactose, glycerol, galactose, saccharose, sorbose, lactic acid or acetic acid), the alcohol-oxidase was about one-tenth of the activity found in cells grown on methanol alone. These carbon sources together with methanol yielded far better synthesis of alcohol-oxidase than in case of carbon sources alone. Especially, the alcohol-oxidase activity of the cells grown on lactose or lactic acid together with methanol was far better or similar than that of cells grown on methanol alone. The synthetic activity of alcohol-oxidase of Hansenula polymoypha CBS 4132 was the strongest among the three strains tested in every respect.

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Synthesis of Alcohol-oxidase in Pichia pastoris on Various Carbon Sources (여러가지 탄소원에 의한 Pichia pastoris의 Alcohol-oxidase 생성)

  • Lee, Myung-Suk;Hur, Sung-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.18 no.4
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    • pp.435-443
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    • 1989
  • The regulation of the synthesis of alcohol-oxidase(E. C. 1. 1. 3. 13) was investigated in the methanol-utilizing yeasts during growth on different carbon sources. For this experiment, Pichia pastoris CBS 2612 and Pichia pastoris CBM 10 were cultured in mineral salt medium by changing its carbon sources. The production of alcohol-oxidase was varied by the carbon sources. For example, alcohol-oxidase was undetectable in all strains submitted to the test in the medium with glucose, but its production was rapidely increased when the carbon source was changed from glucose to methanol after 48hrs of incubation. Moreover, this enzyme was not synthesized during growth on the primary aliphatic alcohols alone(ethanol, propanol, butanol or pentanol) or on the mixed substrates(0.5% methanol+0.5% primary aliphatic alcohols). When cells were grown on the various carbon sources(glucose, xylose, lactose, glycerol, galactose, saccharose, sorbose, lactic acid or acetic acid), The alcohol-oxidase activity was detected a very little amounts. These carbon sources together with methnol yieled far better synthesis of alcohol-oxidase than in case of carbon sources alone. Especially, the alcohol-oxidase activity of the cells grown on sorbose, lactose or lactic acid together with methanol was far better or similar than that of cells grown on methanol alone. The apparent Km values for the methanol of Pichia pastoris CBS 2612 and Pichia pastoris CBM 10 enzymes were 1.92 and 210 mM, respectively. It is also active towards alcohols of shorter alkyl-chain length than $C_7$, insaturated alcohols(allylalcohol, crotyl-alcohol) and secondary alcohols (iso-amylacohol, iso-butylalcohol). The affinity of alcohol-oxidase for this alcohols decreased with the increasing length of the alkyl-chain.

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Geostatistical Integration of Ground Survey Data and Secondary Data for Geological Thematic Mapping (지질 주제도 작성을 위한 지표 조사 자료와 부가 자료의 지구통계학적 통합)

  • Park, No-Wook;Jang, Dong-Ho;Chi, Kwang-Hoon
    • Korean Journal of Remote Sensing
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    • v.22 no.6
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    • pp.581-593
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    • 2006
  • Various geological thematic maps have been generated by interpolating sparsely sampled ground survey data and geostatistical kriging that can consider spatial correlation between neighboring data has widely been used. This paper applies multi-variate geostatistical algorithms to integrate secondary information with sparsely sampled ground survey data for geological thematic mapping. Simple kriging with local means and kriging with an external drift are applied among several multi-variate geostatistical algorithms. Two case studies for spatial mapping of groundwater level and grain size have been carried out to illustrate the effectiveness of multi-variate geostatistical algorithms. A digital elevation model and IKONOS remote sensing imagery were used as secondary information in two case studies. Two multi-variate geostatistical algorithms, which can account for both spatial correlation of neighboring data and secondary data, showed smaller prediction errors and more local variations than those of ordinary kriging and linear regression. The benefit of applying the multi-variate geostatistical algorithms, however, depends on sampling density, magnitudes of correlation between primary and secondary data, and spatial correlation of primary data. As a result, the experiment for spatial mapping of grain size in which the effects of those factors were dominant showed that the effect of using the secondary data was relatively small than the experiment for spatial mapping of groundwater level.

Situation of Geological Occurrences and Utilization, and Research Trends of North Korean Coal Resources (북한 석탄 자원의 부존 및 활용현황과 연구동향)

  • Sang-Mo Koh;Bum Han Lee;Otgon-Erdene Davaasuren
    • Economic and Environmental Geology
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    • v.57 no.3
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    • pp.281-292
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    • 2024
  • North Korea relies heavily on coal as the primary energy source, playing an important role in all energy demand sectors except for the transportation sector. Approximately half of the total electricity is generated through coal-fired power plants, and coal is used to produce heat and power for all industrial facilities. Furthermore, coal has been a significant contributor to earning foreign currency through long-term exports to China. Nevertheless, since the 1980s, indiscriminate mining activities have led to rapid depletion of coal production in most coal mines. Aging mine facilities, lack of investment in new equipment, shortages of fuel and electricity, difficulties in material supply, and frequent damage from flooding have collectively contributed to a noticeable decline in coal production since the late 1980s. North Korea's coal deposits are distributed in various geological formations from the Proterozoic to the Cenozoic, but the most critical coal-bearing formations are Ripsok and Sadong formations distributed in the Pyeongnam Basin of the Late Paleozoic from Carboniferous to Permian, which are called as Pyeongnam North and South Coal Fields. Over 90% of North Korea's coal is produced in these coal fields. The classification of coal in North Korea differs from the international classification based on coalification (peat, lignite, sub-bituminous coal, bituminous coal, and anthracite). North Korean classification based on industrial aspect is classified into bituminous coal, anthracite, and low-grade coal (Chomuyeontan). Based on the energy factor, it is classified into high-calorie coal, medium calorie coal, and low-calorie coal. In North Korea, the term "Chomuyeontan" refers to a type of coal that is not classified globally and is unique to North Korea. It is a low-grade coal exclusively used in North Korea and is not found or used in any other country worldwide. This article compares North Korea's coal classification and the international coal classification of coal and provides insights into the geological characteristics, reserves, utilization, and research trends of North Korean coal resources. This study could serve as a guide for preparing scientific and industrial agendas related to coal collaboration between North Korea and South Korea.

Instrumentation Failure after Partial Corpectomy with Instrumentation of a Metastatic Spine

  • Park, Sung Bae;Kim, Ki Jeong;Han, Sanghyun;Oh, Sohee;Kim, Chi Heon;Chung, Chun Kee
    • Journal of Korean Neurosurgical Society
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    • v.61 no.3
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    • pp.415-423
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    • 2018
  • Objective : To identify the perioperative factors associated with instrument failure in patients undergoing a partial corpectomy with instrumentation (PCI) for spinal metastasis. Methods : We assessed the one hundred twenty-four patients with who underwent PCI for a metastatic spine from 1987 to 2011. Outcome measure was the risk factor related to implantation failure. The preoperative factors analyzed were age, sex, ambulation, American Spinal Injury Association grade, bone mineral density, use of steroid, primary tumor site, number of vertebrae with metastasis, extra-bone metastasis, preoperative adjuvant chemotherapy, and preoperative spinal radiotherapy. The intraoperative factors were the number of fixed vertebrae, fixation in osteolytic vertebrae, bone grafting, and type of surgical approach. The postoperative factors included postoperative adjuvant chemotherapy and spinal radiotherapy. This study was supported by the National Research Foundation grant funded by government. There were no study-specific biases related to conflicts of interest. Results : There were 15 instrumentation failures (15/124, 12.1%). Preoperative ambulatory status and primary tumor site were not significantly related to the development of implant failure. There were no significant associations between insertion of a bone graft into the partial corpectomy site and instrumentation failure. The preoperative and operative factors analyzed were not significantly related to instrumentation failure. In univariable and multivariable analyses, postoperative spinal radiotherapy was the only significant variable related to instrumentation failure (p=0.049 and 0.050, respectively). Conclusion : When performing PCI in patients with spinal metastasis followed by postoperative spinal radiotherapy, the surgeon may consider the possibility of instrumentation failure and find other strategies for augmentation than the use of a bone graft for fusion.

Sulfur Isotope Study on the Wolf River Batholith, Wisconsin in U.S.A. (미국 위스콘신주의 올프리버 저반에 대한 황동위원소 연구)

  • Sun-Joon Kim;Yuch-Ning Shieh
    • The Journal of the Petrological Society of Korea
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    • v.4 no.2
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    • pp.134-143
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    • 1995
  • Sulfur isotope compositions have been determined for the granitic and related rocks from the Wolf River Batholith, Wisconsin in U.S.A. Sulfur content and isotope composition of granitic rocks of the Wolf River Ratholith range from 30 to 140 ppm and from 1.1 to 6.5 permil respectively, and are considered to be magmatic. Sulfur content and isotope composition of the Penokean plutonic rocks, surrounding country rocks, range from 31to 381 ppm and from -1.7 to 7.2 permil respectively. The positive correlation observed between sulfur and oxygen isotope data of granitic rocks and the Penokean plutonic rocks may be due to the assimilation of the Penokean plutonic rocks by a primary magma of deep-crustal origin, or to mixing at depth, of a primary magma with another magma having higher ${\delta}^{18}O$ and ${\delta}^{34}S$.

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Investigation of Properties of Synthetic Microparticles for a Retention and Drainage System

  • Lee, Sa-Yong;Hubbe Martin A.;Park, Sun-Kyu
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2006.06a
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    • pp.61-66
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    • 2006
  • Over the past 20 years there has been a revolution involving the use of nano or macro size particles as drainage and retention systems during the manufacture of paper. More recently a group of patented technologies called Synthetic Mineral Microparticles (SMM) has been invented and developed. This system has potential to further promote the drainage of water and retention of fine particles during papermaking. Prior research, as well as our on preliminary research showed that the SMM system has advantages in both of drainage and retention compared with montmorillonite (bentonite), which one of the most popular materials presently used in this kind of application. In spite of the demonstrated advantages of this SMM system, the properties and activity of SMM particles in the aqueous state have not been elucidated yet. Streaming current titrations with highly charged polyelectrolytes were used to measure the charge properties of SMM and to understand the interactions among SMM particles, fibers, fiber fines, and cationic polyacrylamide (cPAM) as a retention aid. It was found that pH profoundly affects the charge properties of SMM, due to the influence of Al-ions and the Si-containing particle surface. SEM pictures, characterizing the morphology, geometry and size distribution of SMM, showed an broad distribution of primary particle size. Dilution of SMM mixturee appeared to wash out particles smaller than 100 nm from the surface of larger particles, which themselves appeared to be composed of fused primary particles. DSC thermoporometry was used to measure the size distribution of nanopores within SMM particles.

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The Characteristics of Organic Matter in the Quaternary Sediments from ODP Leg 127 Site 794A, East Sea (동해 ODP Leg 127 Site 794A에서 채취한 제4기 퇴적물의 유기지화학적 특성 연구)

  • Lee Sang Il;Lee Young-Joo;Kim Ji Hoon;Oh Jae Ho;Yun HyeSu
    • Economic and Environmental Geology
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    • v.38 no.6 s.175
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    • pp.707-716
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    • 2005
  • Organic geochemical analyses including Rock-Eval pyrolysis, elemental analysis and stable carbon isotope analysis were performed to evaluate the characteristics of organic matter in the ODP Leg 127 Site 794A sediments and to understand paleoceanographic changes. Based on the TOC contents, C/N ratio, HI vs. OI, $\delta^{13}C_{org}$ and C/S ratio, results imply that dark layers containing a large amount of terrigenous organic matter were deposited under the suboxic/anoxic conditions, whereas the light layers containing largely marine organic matter were deposited under the oxic conditions. These results indicate that increasing surface-productivity by the input of a large amount of terrigenous organic matter from adjacent continent led to the deposition of dark layers during the interglacial highstands, whereas marine primary production and dilution caused by Kosa from the China desert area led to the deposition of light layers with the decreased to terrigenous organic matter during the glacial lowstands.

Gold Mineralization of the Sepola District in Mali, Africa: Occurrence Characteristics of Gold and Fluid Inclusion Study (아프리카 말리 세폴라 지역의 금광화작용:금의 산출특성 및 유체포유물연구)

  • Heo, Chul-Ho
    • Economic and Environmental Geology
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    • v.42 no.3
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    • pp.167-175
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    • 2009
  • The geology of Sepola district in Mali is consisted of Birrimian group with metasedimentary rocks of lower Proterozoic and volcanoclastics, and later intrusive igneous rocks. Ore diposit in this district has the characteristics of vein- and disseminated-type gold deposit which was formed by infilling the secondary fracture zones related to the large-scale fault zone of NW direction within Birrimian group. It is confirmed as promising that Barani district has the gold grade of 0.53${\sim}$9.21 g/t with the extension of 1.3 km and width of 1 to 20.1 m. The ore mineralogy is simple with electrum, pyrite and galena. Fineness of gold grain ranges from 848 to 915(average 891) indicating mesothermal to hypothermal environment. Fluid inclusions are classified as liquid-rich type I. gas-rich type II and liquid-$CO_2$ bearing type III. Primary and pseudosecondary inclusions homogenize from 236 to 393$^{\circ}C$ with salinity of 0.0 to 8.6 wt% NaCl. Secondary inclusions homogenize from 103 to 184$^{\circ}C$ with salinity of 0.7 to 8.6 wt.%. From the relationship between homogeniztion temperature and salinity, it may be thought that auriferous fluid experienced dilution and cooling through inflow of meteoric water after fluid unmixing derived from pressure decrease in the temperature range of 400 to 250$^{\circ}C$. From the massive occurrence of quartz vein, simple mineralogy with paucity of sulfide, and presence of liquid-$CO_2$ bearing with high homogenization temperature, it is thought that gold mineralization in Sepola district correspods to the mesothermal to hypothermal ore deposit.

Interpretation of Geophysical Well Logs from Deep Geothermal Borehole in Pohang (포항 심부 지열 시추공에 대한 물리검층 자료해석)

  • Hwang, Se-Ho;Park, In-Hwa;Song, Yoon-Ho
    • Geophysics and Geophysical Exploration
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    • v.10 no.4
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    • pp.332-344
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    • 2007
  • Various geophysical well logs have been made along the four deep wells in Pohang, Gyeongbuk. The primary focus of geophysical well loggings was to improve understanding the subsurface geologic structure, to evaluate in situ physical properties, and to estimate aquifer production zones using fluid temperature and conductivity gradient logs. Especially natural gamma logs interpreted with core logs of borehole BH-1 were useful to discriminate the lithology and to determine the lithologic sequences and boundaries consisting of semi-consolidated Tertiary sediments and intrusive rocks such as basic dyke and Cretaceous sediments. Cross-plot of physical properties inferred from geophysical well logs were used to identify rock types such as Cretaceous sandstone and mudstone, Tertiary sediments, rhyolite, and basic dyke. The temperature log indicated $82.51^{\circ}C$ at the depth of 1,981.3 meters in borehole BH-4. However, considering the temperature of borehole BH-2 measured under stable condition, we expect the temperature at the depth in borehole BH-4, if it is measured in stable condition, to be about 5 or $6^{\circ}C$ higher. Several permeable fractures also have been identified from temperature and conductivity gradient logs, and cutting logs.