• 대한토목학회논문집
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• 제33권4호
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• pp.1651-1664
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• 2013

도로포장의 파손과정에는 다양하고 복합적인 원인에서 비롯되는 불확실성이 포함되어 있어 정확한 해석이 쉽지 않다. 이로 인해 최근에는 결정론적 모형보다는 확률이론이 보다 많이 활용되고 있으나, 파손의 전체적 특성만을 설명하는 일반적인 분석방안으로는 포장파손특성의 변화과정에 대해 구체적인 정보를 제공하기 어렵다. 이에 본 연구에서는 포장파손과정을 상태와 시간기준으로 분해함으로써 지역적으로 이질성을 띄는 포장파손속도와 그 분산에 대해 구체적으로 파악하고자 하였다. 또한, 분해된 확률과정을 다시 조합하는 과정을 통해 포장의 기대수명과 불확실성을 예측해 보았다. 실증분석을 위해 일반국도포장관리시스템에서 2003년부터 2010년까지 수집된 균열률 자료를 활용하였다. 이러한 시도들은 자산관리의 주요기법 중 하나인 생애주기비용분석의 신뢰성을 높일 수 있으며, 파손속도의 변화과정에 대한 이해가 필수적인 예방적 유지보수전략에 관한 기반연구로써도 중요한 의의가 있다.

• 한국발생생물학회지:발생과생식
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• 제19권3호
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• pp.119-126
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• 2015

The suitable feeder cell layer is important for culture of embryonic stem (ES) cells. In this study, we investigated the effect of two kinds of the feeder cell, MEF cells and STO cells, layer to mouse ES (mES) cell culture for maintenance of stemness. We compare the colony formations, alkaline phosphatase (AP) activities, expression of pluripotency marker genes and proteins of D3 cell colonies cultured on MEF feeder cell layer (D3/MEF) or STO cell layers (D3/STO) compared to feeder free condition (D3/-) as a control group. Although there were no differences to colony formations and AP activities, interestingly, the transcripts level of pluripotency marker genes, Pou5f1 and Nanog were highly expressed in D3/MEF (79 and 93) than D3/STO (61and 77) or D3/- (65 and 81). Also, pluripotency marker proteins, NANOG and SOX-2, were more synthesized in D3/MEF ($72.8{\pm}7.69$ and $81.2{\pm}3.56$) than D3/STO ($32.0{\pm}4.30$ and $56.0{\pm}4.90$) or D3/- ($55.0{\pm}4.64$ and $62.0{\pm}6.20$). These results suggest that MEF feeder cell layer is more suitable to mES cell culture.

• 대한장애인치과학회지
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• 제1권1호
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• pp.18-24
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• 2005

The purposes of this study were 1) to investigate the oral health problem of the disabled according to the handicapped types, 2) to collect the empirical data for developing and establishing the oral health policies for the handicapped, and 3) to find out the major obstacles against the dental services and oral health promotion. The handicapped subjects were collected by designed cluster sampling and interviewed with structured questionnaire in order to measure the oral health knowledge and behaviors. The subjects were attending the special schools and private or public welfare institutions for crippled disorder, encephalopathy disorder, mental retardation, visual disturbance, hearing defect, and developmental disorder. Oral examination was conducted by 13 trained dentists. 1,476 of handicapped people were finally surveyed. The results were as follows; The experiences of visiting dental office during the last one year in 18~64 year-old disabled people were 48.86% in crippled disorder, 52.50% in mental retardation, 58.24% in visual disturbance, 39.29% in hearing defect, respectively. To improve this challenging situation, we should find out the obstacles against the dental service and oral hygiene maintenance by the types of handicap, and develop the oral health policies which could support and advocate the Korean disabled.

• 융합보안논문지
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• 제14권3_2호
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• pp.93-98
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• 2014

최근 서울 시민을 대상으로 한 설문조사에서 환경 문제 해결, 교통 체계 개선과 함께 범죄 예방에 관한 요구가 가장 높게 나타났다. 그 중에서도 가족과 함께하는 주거 지역의 범죄 예방에 대한 요구는 다른 어느 항목보다 더 중요한 것으로 나타났다. 범죄 발생률은 주로 노후화 된 주거 지역에서 높게 나타나는데, 이는 범죄와 물리적인 환경 요인의 관계를 나타내 주는 것이라 할 수 있고, 주변 환경에 대해 적절한 조치가 범죄를 예방할 수 있다는 것으로 이해될 수 있다. 각종 범죄로부터 안전할 수 있도록 하는 다양한 예방법이 있지만 우리 주변의 물리적인 환경을 개선함으로써 범죄를 사전에 차단하고 예방할 수 있다는 CPTED는 이러한 측면에서 가장 주목받고 있는 방법 중 하나이다. CPTED의 기본 원리는 자연감시, 접근통제, 영역성 확보의 기본 원리와 활동성 강화, 유지 관리의 부가 원리로 구성된다. 본 연구의 목적은 도심지 내 노후화 된 공동주택 단지를 대상으로 CPTED의 원리를 적용하여 범죄 발생률을 떨어뜨리고 사전에 예방할 수 있는 방안을 제시하는데 있다.

• Preventive Nutrition and Food Science
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• 제19권3호
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• pp.178-186
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• 2014

Many recent studies have focused on maintaining a healthy life by preventing and/or postponing the aging process. Numerous studies have reported that continuous exposure to reactive oxygen species can stimulate skin aging and that excessive accumulation of fat can cause an impaired skin barrier and tissue structure alterations. Thus, the maintenance of antioxidant homeostasis and the suppression of adipose accumulation are important strategies for skin anti-aging. Here, we prepared three types of extracts [whole juice, acetone-perchloric acid (PCA), and ethanol] from 20 fruits and medicinal herbs native to the Gyeongnam area of Korea. The total phenolic content of each extract was analyzed, and we observed higher total phenolic contents in the medicinal herbs. Consistent with this, the results of the oxygen radical absorbance activity capacity assay indicated that the in vitro antioxidant activities of the medicinal herb extracts were stronger than those of the fruit extracts. The fruits and medicinal herbs had strong effects on cell-based systems, including $H_2O_2$-induced oxidative stress in human keratinocytes and 3T3-L1 lipid accumulation. Nishimura Wase persimmon, Taishu persimmon, wrinkled giant hyssop, sweet wormwood, Chinese cedar, red perilla, tan shen, hiyodori-jogo, and cramp bark may be natural anti-aging materials with effective antioxidant and anti-adipogenic activities. Taken together, our findings may provide scientific evidence supporting the development of functional foods and nutraceuticals from fruits and medicinal herbs.

• Preventive Nutrition and Food Science
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• 제21권4호
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• pp.310-316
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• 2016

In this study, the anti-osteoarthritis effects of Cynanchum wilfordii, Phlomis umbrosa, and Angelica gigas extract (CPAE), observed and confirmed in previously clinical studies were further investigated by in vitro and in vivo studies. Anabolic biomarkers related to healthy cartilage maintenance, such as aggrecan, type II collagen ${\alpha}$-1 (Col2a1), sex determining region Y-box-9 (Sox-9), and catabolic biomarkers related to osteoarthritis, such as cyclooxygenase-2 (Cox-2), matrix metalloproteinase-13 (Mmp13), and nuclear factor kappa-light-chain-enhancer of activated B cells ($Nf{\kappa}b$), were evaluated by quantitative reverse transcriptase polymerase chain reaction and reporter gene assay. In vitro study results showed significant changes in both anabolic and catabolic biomarkers. For anabolic factors, significant changes in the level of aggrecan (P<0.05), Col2a1 (P<0.05), and Sox-9 (P<0.01) activation were shown after treatment of cartilage cells with CPAE (50 ng/mL) with similar efficacy compared to insulin growth factor, the positive control (100 ng/mL). For catabolic factors, significant changes in the inhibition activity of Cox-2 (P<0.05), Mmp13 (P<0.01), and $Nf{\kappa}b$ (P<0.05) were shown for CPAE (50 ng/mL) with similar efficacy compared to Celecoxib, the positive control ($10{\mu}M$). In the in vivo carrageenan-induced paw edema model study results showed that CPAE-treated groups (100 mg/kg) and Celecoxib-treated groups (60 mg/kg) showed comparably significant efficacy of inhibition by 37.1% and 52.1%, respectively. Furthermore, CPAE (200 mg/kg) showed similar effect to Celecoxib (60 mg/kg) with an inhibition rate of 54.3%. This result confirms that CPAE effectively inhibited the inflammation-induced osteoarthritis symptoms.

• 대한방사선기술학회지:방사선기술과학
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• 제38권4호
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• pp.395-401
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• 2015

영상의학과의 흉부엑스선 검사에서 감염관리에 대한 기기의 오염 정도를 세균학적으로 확인하고 균을 동정하여 병원감염의 예방과 감염관리방안을 마련하는데 목적이 있다. 영상의학과 흉부 엑스선 검사에서 환자의 기기 접촉부위인 어깨, 손, 턱, 흉부 측면촬영의 손잡이 부위를 70% 이소프로필 알코올로 소독하기 전과 후를 면봉으로 검체를 채취하여 세균을 동정하여 비교하여 분석하였다. 흉부 엑스선 기기에서 소독전의 측면촬영의 손잡이에서 그람 양성의 Staphylococcus가 분리되었다. 최종동정을 위해 노보바이오신 (nobobiocin) 항생제 시험을 실시하여 민감성으로 Staphylococcus epidermidis로 확정하였다. 흉부촬영 기기에서 측면촬영의 손잡이 부위를 소독 전에 Staphylococcus epidermidis 균이 검출되었다. 흉부엑스선 검사에서 기기를 주기적인 소독 및 멸균을 실시하여 병원감염을 예방하는 관리방안을 마련하여야 한다.

• Biomolecules & Therapeutics
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• 제20권1호
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• pp.68-74
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• 2012

Anthocyanins have received growing attention as dietary antioxidants for the prevention of oxidative damage. Astrocytes, which are specialized glial cells, exert numerous essential, complex functions in both healthy and diseased central nervous system (CNS) through a process known as reactive astrogilosis. Therefore, the maintenance of glial cell viability may be important because of its role as a key modulator of neuropathological events. The aim of this study was to investigate the effect of anthocyanin on the survival of glial cells exposed to oxidative stress. Our results demonstrated that anthocyanin extracts from black soybean increased survival of U87 glioma cells in a dose dependent manner upon oxygen-glucose deprivation (OGD), accompanied by decrease levels of reactive oxygen species (ROS). While treatment cells with anthocyanin extracts or OGD stress individually activated autophagy induction, the effect was signifi cantly augmented by pretreatment cells with anthocyanin extracts prior to OGD. The contribution of autophagy induction to the protective effects of anthocyanin was verifi ed by the observation that silencing the Atg5 expression, an essential regulator of autophagy induction, reversed the cytoprotective effect of anthocyanin extracts against OGD stress. Treatment of U87 cells with rapamycin, an autophagy inducer, increased cell survival upon OGD stress comparable to anthocyanin, indicating that autophagy functions as a survival mechanism against oxidative stress-induced cytotoxicity in glial cells. Our results, therefore, provide a rationale for the use of anthocyanin as a preventive agent for brain dysfunction caused by oxidative damage, such as a stroke.

• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2005년도 춘계 학술대회
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• pp.145-150
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• 2005

방사성 폐기물을 처분장에 처분하기 위해서는 처분 안전성을 확보하여야 한다. 본 연구는 간접측정 방법 중 하나인 물질수지 기법을 이용하여 방사성폐기물의 핵종재고량 평가 프로그램을 개발하였다. 개발 기법의 현장 적용평가를 위하여 고리4호기(9차계획예방정비)를 대상으로 선정하였다. 개발한 평가방법의 검증을 위해 정지수화학처리시 정화계통 내 핵종 제거량 평가자료를 바탕으로 비교평가를 수행하였다. 평가대상 핵종은 Co-60이며, 평가결과 상대오차 $1\%$미만으로 나타났다. 이와 같은 평가결과를 바탕으로 상용원전에서 제시하고 있는 해당기간 발생된 폐기물의 직접 측정 결과와 비교하였고, 그 결과 직접측정 방법에 의한 Co-60의 함유량은 본 연구의 개발기법에서 산출한 값보다 약 $50\%$ 작은 것으로 평가 하였다.

• 한국치위생학회지
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• 제15권6호
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• pp.973-981
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• 2015

Objectives: The purpose of the study is to investigate the correlation with subjective oral health status and food preference in elderly people. Methods: Data were extracted from the fifth Korea National Health and Nutrition Survey 2010-2012. The survey data included general characteristics of the subjects, food preference, and subjective oral health status. Food preference was analyzed by assigning score to 63 food categories. The subjective oral health status consisted of toothache within a month, self-perception of oral health, chewing difficulty, mastication difficulty, and speaking difficulty. Data were analyzed by frequency analysis, descriptive statistics, ${\chi}^2$ test, t-test, and ANOVA using SPSS 22.0 program. Results: Elderly men had a tendency to have a liking for all food categories except for fruit. Difficulty in chewing and mastication was shown in those who had older age, lower education, lower monthly income, and national basic livelihood security. Difficulty in speaking was found in those who had older age, lower education, and national basic livelihood security. Self perception of oral health status was closely related to food preference for pulses and potatoes, fish, vegetables and others(p<0.05). Difficulties in chewing and mastication had relation to all food categories excepting cereals, lipids and sugars(p<0.05). Speaking difficulty were significantly different in pulses, potatoes, meat, eggs, vegetables, fruits, milk, dairy products, and others(p<0.05). Conclusions: Poor subjective oral health status was closely related to food preference. It is necessary to educate that maintenance of good oral health is to ingest the balanced nutrition in the elderly people.