• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.33 no.11
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• pp.65-73
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• 2005

The compressible flow of reactive fluid is investigated by using the transonic small-disturbance (TSD) model and the one-step first-order Arrhenuis chemical reaction. The fluid flow is restricted to dilute premixed reactions with small heat release. The effects of channel shape and Mach number on transonic combustion are studied by numerical analysis. The results show that the channel divergence increases the chemical reaction within the given channel length whereas the channel convergence inhibits the chemical reaction near the outlet and that increasing the inlet flow Mach number at a fixed reaction rate causes the flow acceleration in a diverging channel and the appearance of weak shock waves which do not show in the inert flow case. It also helps to increase the pressure and temperature near the diverging channel outlet and to consume the reactant within the given channel length.

• Journal of Microbiology and Biotechnology
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• v.11 no.2
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• pp.222-228
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• 2001

Vibrio metschnikovii strain RH530 is a non-pathogenic, industrially-important alkaline protease producer which has been isolated from wastewater. In this paper, we report on the transformation of this strain by using the method of electroporation. A field strength of $7.5\;kVcm^{-1}$ and $25\;{\mu}F$, and using a 0.2-cm cuvette, appeared to be the optimal conditions for electroporation of the cells with the recombinant pSBCm plasmid carrying the vapK alkaline protease gene and the ColE1 replicon. Cells were subjected to osmotic shock in order to remove extracelluar DNase, and adding 200 mM of sucrose to electroporation buffer cells showed an increased transformation efficiency. Maximum efficiency of transformation was obtained at an early exponential growth phase. Using all of the conditions mentioned above, we routinely obtained a transformation efficiency of more than $10^4{({\mu}g\;plasmid\;DNA)}^{-1}$. The stability of the plasmid pSBCm in V. metschnikovii RH530 was 25% after 18h of growth (27 generations) in the medium without antibiotic selection. The insertion of the par locus to the pSBCm increased the stability of the plasmid up to 42% without selective pressure. The increase in plasmid stability was accompanied by the increase in the productivity of alkaline protease in the recombinant V. metschnikovii strain RH530. Determining optimal conditions for the transformation of the industrially-important, nonpathogenic Vibrio strain, and the improvement of plasmid stability by introducing the par locus into the high copy number plasmid vector, will allow the development of procedures involved in the genetic manipulation of this strain, particularly for its use in the production of industrial enzymes such as alkaline protease.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.48 no.7
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• pp.497-504
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• 2020

A technique was developed for analysis on sonic boom created by supersonic flight and for prediction of its sound level and atmospheric propagation characteristics. It is of great importance to anticipate sound level of sonic boom because it causes environmental issue. For that purpose, the simplified sonic-boom prediction method was applied to calculate sound pressure according to physical properties and flight information of the object and distance to measurement site, in this study. Propagation characteristics of shock wave emanated from a flying object was analyzed by using line-of-sight vector and ray tracing method which dealt with refraction of wave due to atmospheric density distribution along altitude. Predicted results agreed well with measured data from real flight.

• Journal of the Korean Society of Propulsion Engineers
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• v.3 no.4
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• pp.58-66
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• 1999

The flow characteristics of supersonic ejectors is often subject to compressibility, unsteadiness and shock wave systems. The numerical works carried out thus far have been of one-dimensional analyses or some Computational Fluid Dynamics(CFD) which has been applied to only a very simplified configuration. For the design of effective ejector-pump systems the effects of secondary mass flow on the supersonic ejector flow should be fully understood. In the present work the supersonic ejector-pump flows with a secondary mass flow were simulated using CFD. A fully implicit finite volume scheme was applied to axisymmetric compressible Navier-Stokes equations. The standard two-equation turbulence model was employed to predict turbulent stresses. The results obtained showed that the flow characteristics of constant area mixing tube types were nearly independent of the secondary flow rate, but the flow fields of ejector system with the second-throat were strongly dependent on the secondary flow rate due to the effect of the back pressure near the primary nozzle exit.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2006.11a
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• pp.377-381
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• 2006

This paper describes experimental work to control supersonic jet noise using a mesh screen that is placed at the nozzle exit plane. The mesh screen is a wire-gauze screen that is made of long stainless wires with a very small diameter. The nozzle pressure ratio is varied to obtain the supersonic jets which are operated in a wide range of over-expanded to moderately under-expanded jets. In order to perturb mainly the initial jet shear layer, the hole is perforated in the central part of the mesh screen. The hole size is varied to investigate the noise control effectiveness of the mesh screen. A schlieren optical system is used to visualize the flow fields of supersonic jet with and without the mesh screen device. Acoustic measurement is performed to obtain the OASPL and noise spectra. The results obtained show that the present mesh screen device leads to a substantial suppression of jet screech tones. The hole size is an important factor in reducing the supersonic jet noise. For over-expanded jets, the noise control effectiveness of the mesh screen appears more significant, compared to correctly and under-expanded jets

• KIEAE Journal
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• v.16 no.3
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• pp.121-128
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• 2016

Purpose: The vacuum glazing should constantly retain the gap in vacuum state to maintain high thermal performance. To do so, pillars are used to prevent the glazing from clinging to each other by the atmospheric pressure and therefore surface of the vacuum glazing is consistently affected by residual stress. The vacuum glazing could be applied to curtain wall systems at spandrel area to fulfill a rigorous domestic standard on U-value of the external wall. However, this can lead to high glazing temperature increase by heat concentration at a back panel and finally thermal stress breakage. This study experimentally determined weakness of the vacuum glazing systems on the thermal stress breakage and investigated effect of the residual stress. Method: The experiment first built two scale-down mock-up facilities that replicate the spandrel area in curtain wall, and then installed single low-e glass and vacuum glazing respectively. The two mock-up facilities were exposed to outside to induce the thermal stress breakage. Result: The experiment showed that the temperature occurred the thermal stress breakage was $114.4^{\circ}C$ for the single low-e glass and $118.9^{\circ}C$ for the vacuum glazing respectively. The result also showed the vacuum glazing reached the critical point earlier than the single low-e glass, which means that the vacuum glazing has high potential to occur the thermal shock breakage. In addition, the small temperature difference between two glazing indicates that the residual stress scarcely affects breakage of the vacuum glazing.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.45 no.3
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• pp.241-251
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• 2017

Computational fluid dynamics analysis was performed for the case 3 of the EFD-CFD workshop. Solvers were used for three commercial CFD codes(Star-CCM+, Fluent and CFX) and an open source CFD code(SU2). The grid were generated four types depending on the total cells using commercial grid generation code(Pointwise). Mach number of 0.4 and 0.8, 2 degree angle of attack and Mach number of 0.9, 1 degree angle of attack were calculated. Similar pressure coefficient curve and normal force coefficient were showed from the coarse grid to fine grid of four codes. But there is a difference in the drag coefficient. The position of the shock wave was predicted forward as the discretization order increased in calculations using Star-CCM+ and Fluent. The computation time to converge, Fluent, Star-CCM +, CFX are in order, and SU2 takes much time to converge.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.45 no.3
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• pp.259-267
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• 2017

Flow fields around a KARI-11-180 airfoil, SDM and transonic body are numerically simulated by using an unstructured meshes based compressible flow solver developed at KAIST. RANS equations are solved to analyse the flow fields and Roe's FDS method is adopted to evaluate convective fluxes. Turbulence effect of the flow fields is modeled by a SA model, SST model and ${\gamma}-{\widetilde{Re}}_{{\theta}t}$ model. It is found that smaller drag coefficients are predicted for the KARI-11-180 airfoil when a transition phenomenon is considered and small deviations exist between CFD and EFD results. For the SDM, flow separation is observed at a leading edge and calculated aerodynamic properties show similar tendencies to experimental results. A shock wave on main wings of the transonic body is successfully captured by the present flow solver at a Mach number 0.9. Estimated pressure profiles by means of the present CFD method also agree well with those of wind tunnel results.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.2133-2138
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• 2003

In general, flow entrainment of surrounding gas into a supersonic jet is caused by the pressure drop inside the jet and the shear actions between the jet and the surrounding gas. In the recent industrial applications, like supersonic ejector system or scramjet engine, the rapid mixing of two different gases is important in that it determines the whole performance of the flow system. However, the mixing performance of the conventional circular jet is very low because the shear actions are not enough. The supersonic jet discharging from a petal nozzle is known to enhance mixing effects with the surrounding gas because it produces strong longitudinal vortices due to the velocity differences from both the major and minor axes of petal nozzle. This study aims to enhance the mixing performance of the jet with surrounding gas by using the lobed petal nozzle. The jet flows from the petal nozzle are compared with those from the conventional circular nozzle. The petal nozzles employed are 4, 6, and 8 lobed shapes with a design Mach number of 1.7 each, and the circular nozzle has the same design Mach number. The pitot impact pressures are measured in detail to specify the jet flows. For flow visualization, the schlieren optical method is used. The experimental results reveal that the petal nozzle reduces the supersonic length of the supersonic jet, and leads to the improved mixing performance compared with the conventional circular jet.

• Molecular & Cellular Toxicology
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• v.1 no.2
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• pp.73-77
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• 2005

Nickel is the one of potent environmental, the occupational pollutants and the classified human carcinogens. It is a serious hazard to human health, when the metal exposure. To prevent human diseases from the heavy metals, it is seemingly important that understanding of how nickel exerts their toxicity and carcinogenic effect at a molecular and a genomic level. The process of nickel absorption has been demonstrated as phagocytosis, iron channel and diffusion. Uptaked nickel has been suggested to induce carcinogenesis via two pathways, a direct DNA damaging pathway and an indirect DNA damaging pathway. The former was originated from the ability of metal to generate Reactive Oxygen Species (ROS) and the reactive intermediates to interact with DNA directly. Ni-generated ROS or Nickel itself, interacts with DNAs and histones to cause DNA damage and chromosomal abnormality. The latter was originated from an indirect DNA damage via inhibition of DNA repair, or condensation and methylation of DNA. Cells have ability to protect from the genotoxic stresses by changing gene expression. Microarray analysis of the cells treated with nickel or nickel compounds, show the specific altered gene expression profile. For example, HIF-I (Hypoxia-Inducible Factor I) and p53 were well known as transcription factors, which are upregulated in response to stress and activated by both soluble and insoluble nickel compounds. The induction of these important transcription factors exert potent selective pressure and leading to cell transformation. Genes of metallothionein and family of heat shock proteins which have been known to play role in protection and damage control, were also induced by nickel treatment. These gene expressions may give us a clue to understand of the carcinogenesis mechanism of nickel. Further discussions on molecular and genomic, are need in order to understand the specific mechanism of nickel toxicity and carcinogenicity.