• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.1056-1069
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• 2015

Osmotic pressure is a critical factor for erythritol production with osmophilic yeast. Protein expression patterns of an erythritol-producing yeast, Yarrowia lipolytica, were analyzed to identify differentially-expressed proteins in response to osmotic pressure. In order to analyze intracellular protein levels quantitatively, two-dimensional gel electrophoresis was performed to separate and visualize the differential expression of the intracellular proteins extracted from Y. lipolytica cultured under low (3.17 osmol/kg) and high (4.21 osmol/kg) osmotic pressures. Proteomic analyses allowed identification of 54 differentially-expressed proteins among the proteins distributed in the range of pI 3-10 and 14.4-97.4 kDa molecular mass between the osmotic stress conditions. Remarkably, the main proteins were involved in the pathway of energy, metabolism, cell rescue, and stress response. The expression of such enzymes related to protein and nucleotide biosynthesis was inhibited drastically, reflecting the growth arrest of Y. lipolytica under hyperosmotic stress. The improvement of erythritol production under high osmotic stress was due to the significant induction of a range of crucial enzymes related to polyols biosynthesis, such as transketolase and triosephosphate isomerase, and the osmotic stress responsive proteins like pyridoxine-4-dehydrogenase and the AKRs family. The polyols biosynthesis was really related to an osmotic response and a protection mechanism against hyperosmotic stress in Y. lipolytica. Additionally, the high osmotic stress could also induce other cell stress responses as with heat shock and oxidation stress responses, and these responsive proteins, such as the HSPs family, catalase T, and superoxide dismutase, also had drastically increased expression levels under hyperosmotic pressure.

• International Journal of Naval Architecture and Ocean Engineering
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• v.12 no.1
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• pp.928-942
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• 2020

The pressure responsiveness property of a skirt-cushion system, which is closely related to the overall performance of Air Cushion Vehicles (ACVs), has always been the difficulty and challenging problem involving cushion aerodynamics and flexible skirt dynamics. Based on a widely used bag and finger skirt-cushion system, the pressure responsiveness properties are investigated numerically. The physical process and mechanism are analyzed and a numerical method for evaluating the pressure responsiveness property is proposed. A cushion-skirt information communication platform is also presented for interchanging the force and the skirt configuration between cushion aerodynamics and flexible skirt dynamics. The pressure responsiveness of a typical skirt-cushion system is calculated and the results demonstrate that the pressure responsiveness property helps alleviate the influence of the cushion height changing on the overall performance of ACVs. Finally, the influences of skirt geometrical and cushion aerodynamic parameters on the pressure responsiveness properties are discussed systematically, giving insight into the design of skirt-cushion systems.

• Nuclear Engineering and Technology
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• v.56 no.2
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• pp.485-497
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• 2024

Ring beam is the main anchorage zone of the tendons in the nuclear power prestressed concrete containment vessel (PCCV). Its safety is crucial and has a great influence on the overall performance of PCCV. In this paper, two half-scale ring beams were tested to investigate the mechanical performance of the anchorage zone in the PCCV under multidirectional pressure. The effect of working condition with different tension sequences was investigated. Additionally, a half axisymmetric plane model of the containment was established by the finite element simulation to further predict the experimental responses and propose the local reinforcement design in the anchorage zone of the ring beam. The results showed that the ultimate load of the specimens under both working conditions was greater than the nominal ultimate tensile force. The original reinforcement design could meet the bearing capacity requirements, but there was still room for optimization. The ring beam was generally under pressure in the anchorage area, while the splitting force appeared in the under-anchor area, and the spalling force appeared in the corner area of the tooth block, which could be targeted for local strengthening design.

• Geomechanics and Engineering
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• v.36 no.5
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• pp.417-426
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• 2024

Shield tunneling method is widely used to build tunnels in complex geological environment. Stability control of tunnel face is the key to the safety of projects. To improve the excavation efficiency or perform equipment maintenance, the excavation chamber sometimes is not fully filled with support medium, which can reduce the load and increase tunneling speed while easily lead to ground collapse. Due to the high risk of the face failure under non-fully support mode, the tunnel face stability should be carefully evaluated. Whether compressive air is required for compensation and how much air pressure should be provided need to be determined accurately. Based on the upper bound theorem of limit analysis, a non-fully support rotational failure model is developed in this study. The failure mechanism of the model is verified by numerical simulation. It shows that increasing the density of supporting medium could significantly improve the stability of tunnel face while the increase of tunnel diameter would be unfavorable for the face stability. The critical support ratio is used to evaluate the face failure under the nonfully support mode, which could be an important index to determine whether the specific unsupported height could be allowed during shield tunneling. To avoid of face failure under the non-fully support mode, several charts are provided for the assessment of compressed air pressure, which could help engineers to determine the required air pressure for face stability.

• Nuclear Engineering and Technology
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• v.51 no.3
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• pp.908-914
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• 2019

Fusion power shutdown system (FPSS) is a safety system to stop plasma in case of accidents or incidents. The gas injection system for the FPSS presented in this work is designed to research the flow development in a closed system. As the efficiency of the system is a crucial property, plenty of experiments are executed to get optimum parameters. In this system, the flow is driven by the pressure difference between a gas storage tank and a vacuum vessel with a source pressure. The idea is based on a constant volume system without extra source gases to guarantee rapid response and high throughput. Among them, valves and gas species are studied because their properties could influence the velocity of the fluid field. Then source pressures and volumes are emphasized to investigate the volume flow rate of the injection. The source pressure has a considerable effect on the injected volume. From the data, proper parameters are extracted to achieve the best performance of the FPSS. Finally, experimental results are used as a quantitative benchmark for simulations which can add our understanding of the inner gas flow in the pipeline. In generally, there is a good consistency and the obtained correlations will be applied in further study and design for the FPSS.

• Structural Engineering and Mechanics
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• v.86 no.1
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• pp.139-156
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• 2023

The reinforced concrete lining in the hydraulic pressure tunnel tends to crack during the water-filling process. The lining will be detached from the surrounding rock due to the inner water exosmosis along concrete cracks. From the previous research achievements, the cohesive element is widely adopted to simulate the concrete crack but rarely adopted to simulate the lining-rock interface. In this study, the zero-thickness cohesive element with hydro-mechanical coupling property is not only employed to simulate the traditional concrete crack, but also innovatively introduced to simulate the lining-rock interface. Combined with the indirect-coupled method, the hydro-mechanical coupling algorithm of the reinforced concrete lining in hydraulic pressure tunnels is proposed and implemented in the finite element code ABAQUS. The calculated results reveal the cracking mechanism of the reinforced concrete lining, and match well with the observed engineering phenomenon.

• Wind and Structures
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• v.36 no.1
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• pp.41-60
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• 2023

The effect of cladding panel size on the size reduction factor (SRF) of extreme area-averaging wind pressure (EAWP) on the facades of a high-rise building is often ignored in previous studies. Based on wind tunnel tests, this study investigated the horizontal and vertical correlations of wind pressure on the facade claddings of square-section high-rise buildings. Then, the influencing parameters on the SRF of the EAWP on the cladding panels were analyzed, which were the panel area, panel width, panel length and building width. The results show clear regional distinctions in the correlation of wind pressures on the building facades and the rules of the horizontal and vertical correlations are remarkably different, which causes the cladding size ratio to impact the SRF significantly. Therefore, this study suggests the use of the non-dimensional comprehensive size parameter b^𝜶h^1-𝜶/B (𝜶 is the fitting parameter) determined by the cladding panel horizontal size b, cladding panel vertical size h and the building width B rather than the cladding panel area to describe the variation of the EAWP. Finally, some empirical formula for the SRF of the EAWP on the cladding of a high-rise building is proposed with the nondimensional comprehensive size parameter.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2009.03a
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• pp.14-20
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• 2009

Atmospheric pressure air plasma was applied for treatment of different kinds of natural bamboo fiber to improve their mechanical properties and surface characteristics, which are suitable for adhesion and dyeing. The tensile strength and Young modulus of bamboo fiber were significantly improved; SEM and AFM study show that the surface of fiber became cleaner and rougher after plasma treatment. Plasma treatment caused the cracking, removing of the protective skin of alkali-untreated fiber and etching to form a cleaner and rougher surface. The dyeability of both groups of bamboo fiber which are used for composite and textile purposes is significantly enhanced after treatment.

• Journal of the Korean Society of Physical Medicine
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• v.18 no.4
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• pp.37-45
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• 2023

PURPOSE: The coexistence of age-related skeletal muscle mass loss and obesity poses a substantial health risk for individuals because it combines the detrimental effects of muscle mass reduction associated with aging and the health complications from obesity. This study aimed to identify the incidence rate and key influencing elements among Korean men in their thirties. METHODS: A cross-sectional study involving 934 male participants was performed using complex sampling analysis. Various influencing elements were investigated, including age, height, weight, body mass index, waist circumference, skeletal muscle mass index, smoking and drinking behaviors, systolic and diastolic blood pressure, fasting glucose levels, triglyceride, and cholesterol levels. RESULTS: The incidence rate was 2.90%. The key influencing elements were age, height, weight, body mass index, waist circumference, skeletal muscle index, systolic blood pressure, fasting glucose, triglyceride, and total cholesterol (p < .05). CONCLUSION: This study identified the incidence rate and key influencing element for CALSMO among Korean younger community-dwelling men.

• Wind and Structures
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• v.32 no.2
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• pp.105-114
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• 2021

This paper describes a wind tunnel test on a 1:25 scale model of TTU building with several adjustable openings in order to comprehensively study the characteristics of fluctuating internal pressures, especially the phenomenon of the increase in fluctuating internal pressures induced by tangential flow over building openings and the mechanism causing that. The effects of several factors, such as wind angle, turbulence intensity, opening location, opening size, opening shape and background porosity on the fluctuating internal pressures at oblique wind angles are also described. It has been found that there is a large increase in the fluctuating internal pressures at certain oblique wind angles (typically around 60° to 80°). These fluctuations are greater than those produced by the flow normal to the opening when the turbulence intensity is low. It is demonstrated that the internal pressure resonances induced by the external pressure fluctuations emanating from flapping shear layers on the sidewall downstream of the windward corner are responsible for the increase in the fluctuating internal pressures. Furthermore, the test results show that apart from the opening shape, all the other factors influence the fluctuating internal pressures and the internal pressure resonances at oblique wind angles to varying degrees.