• Proceedings of the Korean Society of Life Science Conference
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• 2002.12a
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• pp.48-60
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• 2002

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the production of granulocytes, macrophages, and white blood cells. The effects of osmotic pressure on secretion of human GM-CSF into the culture medium were investigated in suspension cultures of transgenic tobacco cells. An increase in osmotic pressure caused by the addition of mannitol decreased the cell size index, with the effect being more pronounced when cells were measured wet rather than dry. Increased osmotic pressure enhanced the secretion of hGM-CSF. At 90 g/L mannitol, the maximum concentration tested, hGM-CSF was present in the culture medium at 980 ug/L. As the concentration of mannitol increased, the total amount of protein secreted also increased, but was disproportionately enriched in GM-CSF NaCl, another osmoticum, had very similar effects on cell growth and hGM-CSF production, but did not cause enrichment for hGM-CSF Additionally, protein-stabilizing polymer was added to culture broth to enhance stability of secreted recombinant protein. Finally, above two method were applied together to maximize the productivity.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.29 no.9 s.240
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• pp.1049-1056
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• 2005

A conservative pressure-based finite-volume numerical method has been developed for computing flow and heat transfer by using an unstructured grid system. The method admits arbitrary convex polyhedra. Care is taken in the discretization and solution procedures to avoid formulations that are cell-shape-specific. A collocated variable arrangement formulation is developed, i.e. all dependent variables such as pressure and velocity are stored at cell centers. Gradients required for the evaluation of diffusion fluxes and for second-order-accurate convective operators are found by a novel second-order accurate spatial discretization. Momentum interpolation is used to prevent pressure checkerboarding and the SIMPLE algorithm is used for pressure-velocity coupling. The resulting set of coupled nonlinear algebraic equations is solved by employing a segregated approach, leading to a decoupled set of linear algebraic equations fer each dependent variable, with a sparse diagonally dominant coefficient matrix. These equations are solved by an iterative preconditioned conjugate gradient solver which retains the sparsity of the coefficient matrix, thus achieving a very efficient use of computer resources.

• Journal of the Korean Society of Propulsion Engineers
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• v.6 no.1
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• pp.71-82
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• 2002

A time-iterative compressible Navier-Stokes code is developed to analyze the flowfield of a two-dimensional ejector nozzle system. A parametric study has been made for two controlling parameters, duct to nozzle area ratio and nozzle pressure ratio. Results show that there is an optimum area ratio for an efficient pumping of secondary flow. At high area ratios, a freestream flow directly passes through the mixing duct without giving adequate pumping. While at low area ratios, jet boundary is acting as a blockage to incoming flow. The nozzle pressure ratio variation shows that the pumping rate increases as the pressure ratio increases provided there is no interaction between the shroud wall and the shock cell structure.

• Transactions of the Korean hydrogen and new energy society
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• v.24 no.1
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• pp.70-75
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• 2013

It has fabricated membrane electrode assemblies (MEA) for proton exchange membrane fuel cell by hot-pressing method. The hot-pressing was used for the fabrication of MEA which is composed of commercial platinum electrode on carbon paper. The performance of MEA was studied with different fabrication conditions of temperature, pressure and torque. As the temperature increased, the performance of MEA was increased. and started to decrease l after arrived at the maximum performance of MEA. This is related with good contact between electrode and polymer electrolyte membrane at high temperature and microstructural change at much higher temperature. Similarly, as the pressure increased, the performance of the MEA increased up to highest values and start to decrease. According to the our results, the maximal performance of the MEA was at the temperature of $140^{\circ}C$ and the pressure of $1.5{\times}10^3$ kPa. The optimal torque to assemble the single stack was 3.2 N m.

• International Journal of Automotive Technology
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• v.8 no.2
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• pp.149-154
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• 2007

The engine behavior in a transient condition is important to not only emission regulations but also fuel economy. A fast response gas analyzer can be a useful tool to investigate exhaust gas in a transient operation. It should be designed to analyze gas concentration with a short time constant by a fast sampling module and an appropriate measuring method for each emission element. In this study, a new fast sampling module is introduced and flow analysis is performed by numerical simulation. The analysis has shown the proper operating condition and the sensitivity of the module for practical application. Calculated flow to the sampling module has $0.5{\sim}4%$ error, while backflow toward the expansion tube is expected when pressure in CP (Constant Pressure) chamber is over 0.6 bar. For a stable supply of flow to the optical cell, sample gas pressure should be in the range, $0.35{\sim}1.90$ bar, when the pressure in the CP camber and the optical cell are 0.2 bar and 0.158 bar, respectively.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.10
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• pp.1361-1369
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• 2003

Design performance of hybrid power generation systems, comprised of a gas turbine and an atmospheric pressure molten carbonate fuel cell, has been analyzed. Two different configurations were analyzed and performances were compared. A reference calculation was performed for the design condition of a system under development and simulated results agreed well with the published data. Performances were analyzed in terms of main design parameters including turbine inlet temperature, operating temperature of the fuel cell and pressure ratio. Also examined were the effects of fuel utilization factor and heat exchanger effectiveness. It was found that the relationship between the turbine inlet temperature and the fuel cell temperature should be critically examined to evaluate achievable design performance. Considering current state of the art technologies, a system with the combustor located before the turbine could achieve higher efficiency and specific power than the other system with the combustor located after the turbine.

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.5
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• pp.68-75
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• 2010

The one of the major problems in the development of PEMFC was regarding to the assurance of sealing on stack. The failure on the sealing creates the problems of fuel leakage, mixing, internal combustion, damage on parts and can be a direct reason for the degrading the efficiency of fuel cell. This paper studies on the analytical approach for improving the contacting pressure distribution on the gasket at the evaluation on the sealing of fuel cell stack. So, the assembly analysis on multi layered fuel cell stack was performed. The research on the simplification of finite element model was performed for three dimensional analysis at the multi layered state. The improved contact pressure distribution was obtained through the case studies on gasket for better sealing. In addition, the number of the cell was determined for the effective analysis and the structural characteristics were evaluated based on this research.

• Transactions of the Korean hydrogen and new energy society
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• v.20 no.2
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• pp.125-132
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• 2009

Cylinder shaped air-breathing PEMFC has been developed to have small volume, low contact resistance and better air accessibility to the open cathode. This cylinder shaped design consists of an anode cylinder with helical flow channel and a cathode current collector with slits. The pressure distribution measurement according to the shapes was performed. The test result indicated that cylinder shaped fuel cell has better pressure distribution compared with the planar shaped fuel cell. The better pressure distribution was connected to the higher performance. The maximum power density of cylinder shaped fuel cell was about 20% higher than the planar shaped fuel cell. The maximum power density of the developed cylinder shaped air-breathing PEMFC with dry hydrogen was $220\;mW/cm^2$ and with humidified hydrogen was $293\;mW/cm^2$.

• Journal of the Korean Electrochemical Society
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• v.2 no.3
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• pp.163-170
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• 1999

The effects of the operating conditions in AFC single cells have not been studied in detail. In this study, by using a one-dimensional isothermal model a computational simulation was conducted to investigate the effects of the initial electrolyte concentration and the operating gas pressure. According to the result, the optimum electrolyte concentration at the base-case was found to be within $3.0\~3.5$ M. The variation of the cell performance according to the electrolyte concentration was found to be caused mainly by the charge transfer resistances of both electrodes, Henry's constant and the liquid phase diffusivity of the dissolved gases. It was also found that an increase in operating pressure increased the reaction rates and the solubilities of the gases, which led to a considerable enhancement of the cell performance.

• Proceedings of the IEEK Conference
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• 1999.06a
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• pp.551-554
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• 1999

In this paper, we developed a radial pulse transducer that has strain-gauge cantilever type load cell for total pulse detection on chongu arterial. The transducer consist of load cell and driving electronic circuits. Load cell consist of cantilever and two metal film strain gauge. The Pressure signal from chongu artery is delivered to load cell using artery rider that attached to cantilever Therefore the pressure signal convert to voltage signals by the developed transducer As the results of experiment, the developed transducer has very good linearity at pressure to voltage conversion. The total pulse detection transducer can detected three kinds of chongu artery pulse with conveniently.