• 한국식품저장유통학회지
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• 제8권2호
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• pp.187-192
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• 2001

Amino acid composition of proteins in Italian millet, Common millet and sorghum were invstigated by HCI hydrolysis method. The optimum condition was obtained by hydrolysis at 110$\^{C}$ for 24hr. As major amino acids from protein hydrolyzate, the content of tyosine, arginine and phebylalanine were 7.06%, 6.79% and 6.44%, respectively. The content of glutamic acid in Common millet, Italian millet and Sorghum were 5.73%, 5.64% and 5.46%, respectively. Glycine content was about 2.93% in three samples. Contents of crude protein and pure protein in Italian millet, Common millet and sorghum were determined by micro-kjeldahl method. Crude protein contents were slightly higher than that of pure protein. Protein content of sorghum was higher than those of Italian millet and Common millet. For SDS-PAGE analysis, Italian millet showed more soluble proteins including 50kDa, 30kDa and smaller proteins than other cereals. In particular, Common millet and Sorghum only solubilized proteins less than 15kDa.

• 한국식품저장유통학회지
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• 제8권2호
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• pp.224-230
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• 2001

To develop a material for functional food, the fraction Ⅰ,II and Ⅲ of onion extract were separated by Sephadex LH-20 column. The revealed purification levels of each fraction were 5.2%-6.5%, 12.2%-14.5% and 82.6%-86.7%, respectively. A functional activity test was performed with fraction Ⅲ. The ACE inhibition activity was about 50% at 5mg and about 80% at 10mg. The XOase inhibition activity of Korean Changnyong Daego was 84%, which was the highest. At the experiment of tyrosinase inhibition was detected 21-36% at 10mg. The cholesterol dose in mice blood was as low as low as 79$\pm$5(mg/100 mL) after the intake of fraction Ⅲ. Cholestrol dose in liver was decreased to below 50%. The results show that Sephadex LH-20 column method was very useful as a fraction method for the development of functional food material nsing onions.

• 농촌지도와개발
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• 제17권4호
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• pp.897-918
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• 2010

This paper researches conservative value evaluation of rural tourism villages with "CVM(Contingent Valuation Method)" focusing on visitor to offer the guide for preservation and management in landscape of rural tourism villages based on critical consciousness for conservation and utilization by regional development. The option value is 37,624won(36.7%), the bequest value is 34,856won(34.0%), existence value is 30,073 won(29.3%), and overall average willingness to pay of conservation value is 102,517won(100.0%) for conservative value of sesim-village in Kyung-Ju. The results show that landscape of rural tourism villages has great value historically and culturally. In conclusion, it suggested that planners and staffs of rural tourism villages recognize that it is positively necessary to be considered not only multiple landscape index but also conservation policy in process of development planning in rural tourism villages.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2009년도 춘계 학술발표회
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• pp.961-965
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• 2009

Using much electric resistivity survey to design or preservation administration of facilities. However, By survey method that electric resistivity survey grasps special quality in base by potential difference creating artificial current in underground, Is influenced in electric resistance propensitys of various underground utilities in base. This Study selects dam 1 place that could grasp well underground situation and studied electricity interference effect by effect of underground utilities. Grasp underground utilities of dam interior, and control and observed accuracy with survey wave because various pole space. Through this Study, wish to present survey method of electric resistivity survey at design or survey of preservation administration dimension after with drawn result.

• 한국식품저장유통학회지
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• 제4권1호
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• pp.45-51
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• 1997

In our studies on the role of $\beta$-galactosidase in fruit softening, significant difficulty, was encountered in our attempts to extract RNA from persimmon(Diospyros kaki L. cv. Fuyu) fruit due to astringency and tannin content. Initial, unsuccessful RNA extractions involved methods using guanidinium isothiocyanate/CsCl with and without polyvinylpyrrolidone(PVP), phenol/sodium lauryl sulfate(SDS), guanidinium hydrochloride, as well as polysomal RNA purification method that used 0.2 M Tris-HCI (pH 9.0) containing KCI, Mg-acetate, EDTA, $\beta$-mercaptoethanol, and sucrose. A method was devised which employed treatment of fruit with CO2 gas to diminish astringency prior to RNA extraction, followed by extraction of tissue powders with Proteinase K extraction buffer containing PVP and ascorbate at an alkaline pH. This procedure resulted in the removal of tannins and other polyphenolics and extraction of relatively large amount of high-quality RNA suitable for cDNA library construction and polymerase chain reaction(PCR). Futhermore, the procedure does not use the toxic and corrosive chemical guanidinium isothiocyanate or require ultracentrifugation.

• International Journal of Internet, Broadcasting and Communication
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• 제11권1호
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• pp.39-46
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• 2019

This paper proposes an adaptive threshold watershed algorithm, which is the method used for image segmentation and boundary detection, which extends the region on the basis of regional minimum point. First, apply adaptive thresholds to determine regional minimum points. Second, it extends the region by applying adaptive thresholds based on determined regional minimum points. Traditional watershed algorithms create over-segmentation, resulting in the disadvantages of breaking boundaries between regions. These segmentation results mainly from the boundary of the object, creating an inaccurate region. To solve these problems, this paper applies an improved watershed algorithm applied with adaptive threshold in regional minimum point search and region expansion in order to reduce over-segmentation and breaking the boundary of region. This resulted in over-segmentation suppression and the result of having the boundary of precisely divided regions. The experimental results show that the proposed algorithm can apply adaptive thresholds to reduce the number of segmented regions and see that the segmented boundary parts are correct.

• 한국전통조경학회지
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• 제40권1호
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• pp.65-73
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• 2022

본 논문은 굉촌 고촌락의 특성과 변화과정을 통해 변화된 굉촌 역사문화공간의 보존을 위한 보호구역의 재설정 방안을 분석하였다. 이를 위해 굉촌과 관련한 고문헌, 고화, 각종 보호 관련 정책자료를 분석하고 인터뷰와 현지조사를 실시하여 굉촌 내 보존지역과 활용지역 현황을 파악하고 이를 통해 보존지역의 재설정 문제를 살펴보았다. 그 결과 다음과 같은 결론을 도출하였다. 첫째, 굉촌은 유교, 불교, 도교사상의 영향을 받아 조성된 촌락으로 공간구성은 유교의 위계에 따라 조성되었다. 이로 인해 중앙정부의 보존이 존재하지 않았음에도 선조들의 유산이 계승 보존될 수 있었다. 둘째, 굉촌 고촌락은 고대부터 이어져온 문물의 개념으로 인식되어온 협의적 보존의 개념이 적용되어 왔으나 대약진운동과 문화대혁명 등으로 인하여 촌락 공간의 변화를 가져왔다. 이후 굉촌 보호계획을 계기로 신축 및 증축 등의 규제를 통하여 고건축물, 수체계, 수림 등이 보존되었으나 지속된 개발 압력과 개혁개방으로 인한 원주민의 생활 변화로 보존지역 내의 개발이 진행되고 있다. 셋째, 고촌락 원주민들은 유산에 대한 가치 인식은 높았으나 생활의 개선과 수익 창출을 위한 방안 및 이를 위한 촌락의 적극적인 활용을 요구하였다. 넷째, 노거수로 이루어진 수림은 복원이 진행되고 있으나 고택 내 정원들은 활용을 위하여 원림공간이 축소되거나 변형되는 현상을 보이고 있었다. 도로는 본래 고 촌락 진입구인 서측 지역에서 확장된 남측지역에 다리와 주차장이 신설되어 기존 진출입구의 변화를 가져왔다. 이는 관광객의 진출입이 편리하도록 도로체계를 변형한 것으로 고촌락 공간변화의 주요 원인으로 나타났다. 다섯째, 기존의 보존지역을 월소를 중축으로 하는 보존 루트로 재설정하여 보존을 진행하는 한편 주변 지역을 직접·간접체험공간으로 설정하여 각 공간의 위계에 따른 보존과 활용을 교차하여 보존 가운데 활용이 진행되어야 한다.

• 한국수정란이식학회지
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• 제28권2호
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• pp.113-119
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• 2013

The study focuses on the quality assessment of Black Bengal buck semen preserved at chilled condition. In this in vitro trial, collected semen from Black Bengal bucks was preserved at chilling temperature ($4{\sim}5^{\circ}C$) in tris-glucosecitrate yolk medium of 1:5 ratios for four days. Artificial Vagina (AV) method was utilized to collect semen from buck. General evaluation of semen includes the color, mass activity and density were measured by direct visual examination. However, computer-assisted sperm analysis (CASA) and phase contrast microscopy were used to figure out the motility (%), hyper-activated (HYP) motility (%) and number of abnormal spermatozoa (%) initially, and at every 24 h intervals. The result revealed that spermatozoa preserved at chilling temperature showed significantly (P<0.05) lower motility and HYP motility with the progression of preservation. The number of phenotypically abnormal spermatozoa significantly (P<0.05) increased following preservation. Although significant positive correlation (r=0.945; P<0.05) was existed between % motile and % HYP motile spermatozoa however, the % of morphologically abnormal spermatozoa was negatively correlated with % motile (r=-0.997; P<0.05) and % HYP motile spermatozoa (r=-0.946; P<0.01). Therefore, we concluded that the quality of chilled semen progressively losses its viability and doesn't remain useable after certain period of preservation with respect to its motility and morphology.

• Journal of Chest Surgery
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• 제29권10호
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• pp.1066-1075
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• 1996

한국산 잡견을 대상으로 세 종류의 심정지액으로 심장을 정지 보존 후,심장을 적출하여 $0^{\circ}C$에서 보존하면서 일정한 시간별로 6회에 걸쳐 좌심실 근육조직을 채취하여 -75$^{\circ}C$ 냉동고에 저장한 후,초고속 액체 크로마토그라피법으로 purine metabolites를 측정하였다. UW 용액(UWS)군의 ATP 농도는 St. Thomas 용액 (5751군과 혈성 심정 지액 (BCPS)군에 비하여 높으나, 575 군과 BCPS 군 간에는 특이한 차이가 없었다. UWS 군과 BCPS 군의 ADP 농도가 4,8, 12, 24 시간대의 575 군보다 높지만, UWS 군과 BCPS 군 사이에는 특이한 차이가 없었다. AMP 농도는 세군 모두에서 변화가 많지 않았고, adenosine, inoslne, hypoxanthlnc 농도는 시간 경과 에 따라 점차 증가하였고, xanthine의 농도는 매우 소량이어서 비교가 불가능하였다. 본 연구 결과, UW용액이 장시간의 심근보존능력에는 아직 문제점이 있으나, 전신 저체온법을 동시에 시행한 St. Thomas 용액이나 혈성 용액 보다는 보다 우수한 보존용액으로 사료된다.

• 생약학회지
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• 제49권2호
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• pp.172-181
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• 2018

This study was designed to investigate the stability for chemical contents and biological activities of Mahwang-tang (MT) depending on the preservation temperature and periods. MT decoction pouches were preserved for 3 months at room temperature (RT, $23{\pm}1^{\circ}C$) and refrigeration ($4^{\circ}C$). To evaluate the stability of MT decoction, pH and sugar content were estimated. High-performance liquid chromatography analysis were performed to quantify the contents of marker compounds in MT. Anti-inflammatory effects of MT were evaluated to suppress the generation of nitric oxide, prostaglandin $E_2$ and cytokines (tumor necrosis $factor-{\alpha}$ and interleukin-6) in the RAW 264.7 cells stimulated with lipopolysaccharide. Total antioxidant capacity of MT was determined by ABTS radical scavenging activity. The pH of storage method and period showed a tendency to decrease gradually with time. There were no changes in sugar content depending on the preservation temperature and periods of MT decoction. Among the major components of MT, cinnamaldehyde was reduced time-dependently for 3 months of storage at RT. The inflammatory effect and antioxidant capacity of MT were reduced time-dependently at both RT and $4^{\circ}C$. Our results suggest that the preservation period of MT decoction is recommended in refrigeration within 3 months or less rather than at RT.