• Title/Summary/Keyword: Preservation method

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Closed vitrification of mouse oocytes using the CryoLogic vitrification method: A modification that improves developmental competence

  • Jo, Jun Woo;Jee, Byung Chul;Suh, Chang Suk;Kim, Seok Hyun
    • Clinical and Experimental Reproductive Medicine
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    • v.40 no.4
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    • pp.148-154
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    • 2013
  • Objective: To compare the mouse oocyte vitrification outcomes of the CryoLogic vitrification method (CVM) and the conventional open method using a Cryotop. Two CVM methods (original CVM and modified CVM) were tested. Methods: Mature oocytes obtained from female BDF-1 mice were vitrified by two-step exposure to equilibrium and vitrification solutions. Three vitrification protocols were tested on three groups: the CVM-kit, modified CVM, and Cryotop groups. After exposure to the two solutions, the oocytes were vitrified. After warming, the oocytes were fertilized in vitro, and the embryo development was assessed. Blastomeres positive for caspase were counted using an in situ assay kit. The spindle morphology and chromosome configurations of warmed vitrified oocytes were also assessed. Results: The modified CVM and Cryotop groups showed similar developmental capacities, and similar proportions of cells with intact spindles and chromosome configurations. The modified CVM protocol was superior to the original CVM protocol for developmental competence and intact spindle preservation. However, the CVM group showed a relatively higher number of apoptotic cells in blastocysts. Conclusion: Closed vitrification using the modified CVM protocol may be used as an alternative to the conventional open method, but strategies to decrease apoptosis in the blastomere need to be investigated.

Speaker Adaptation Performance Evaluation in Keyword Spotting System (500단어급 핵심어 검출기에서 화자적응 성능 평가)

  • Seo Hyun-Chul;Lee Kyong-Rok;Kim Jin-Young;Choi Seung-Ho
    • MALSORI
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    • no.43
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    • pp.151-161
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    • 2002
  • This study presents performance analysis results of speaker adaptation for keyword spotting system. In this paper, we implemented MLLR (Maximum Likelihood Linear Regression) method on our middle size vocabulary keyword spotting system. This system was developed for directory services of universities and colleges. The experimental results show that speaker adaptation reduces the false alarm rate to 1/3 with the preservation of the mis-detection ratio. This improvement is achieved when speaker adaptation is applied to not only keyword models but also non-keyword models.

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Geometrically nonlinear elastic analysis of space trusses

  • Tin-Loi, F.;Xia, S.H.
    • Structural Engineering and Mechanics
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    • v.7 no.4
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    • pp.345-360
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    • 1999
  • A general framework for the nonlinear geometric analysis of elastic space trusses is presented. Both total Lagrangian and finite incremental formulations are derived from the three key ingredients of statics, kinematics and constitutive law. Particular features of the general methodology include the preservation of static-kinematic duality through the concept of fictitious forces and deformations, and an exact description for arbitrarily large displacements, albeit small strain, that can be specialized to any order of geometrical nonlinearity. As for the numerical algorithm, we consider specifically the finite incremental case and suggest the use of a conventional, simple and flexible arc-length based method. Numerical examples are presented to illustrate and validate the accuracy of the approach.

A Study on Changes of Traditional Wall Technique (전통 벽체 기법 변화에 관한 연구)

  • Cho, Young-Min
    • Journal of architectural history
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    • v.23 no.4
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    • pp.47-56
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    • 2014
  • Traditional wall is ground coat, twice plater, last plaster by such a process has made a wide variety of used materials and techniques are accordingly diverse. These traditional techniques handed down the traditional walls of the techniques, rather than coming to the lowered cut off at any moment from now when the technique is used, not knowing whether to be used. Repair and restore cultural property maintenance work, it is not only the outward form hiding inside it is a technique to keep the preservation of cultural assets can be called true. When viewed from the side walls of these conventional techniques to reveal changes in the traditional process by looking at the changes in technology with traditional techniques for cause shall be made in stock. This paper is from the late Joseon Japanese occupation of techniques ranging from traditional wall to reveal the change process to its current significance of cultural property repair method to be helped.

Direction Information Concerned Algorithm for Removing Gaussian Noise in Images

  • Gao, Yinyu;Kim, Nam-Ho
    • Journal of information and communication convergence engineering
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    • v.9 no.6
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    • pp.758-762
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    • 2011
  • In this paper an efficient algorithm is proposed to remove additive white Gaussian noise(AWGN) with edge preservation. A function is used to separate the filtering mask to two sets according to the direction information. Then, we calculate the mean and standard deviation of the pixels in each set. In order to preserve the details, we also compare standard deviations between the two sets to find out smaller one. Corrupted pixel is replaced by the mean of the filtering window's median value and the smaller set's mean value that the rate of change is faster than the other one. Experiment results show that the proposed algorithm outperforms with significant improvement in image quality than the conventional algorithms. The proposed method removes the Gaussian noise very effectively.

FORAGE BREEDING IN TAIWAN - Review -

  • Cheng, Y.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.4 no.3
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    • pp.203-209
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    • 1991
  • Collection, evaluation and preservation of tropical forage grasses, napier grass breeding, tissue culture of pangola grass and alfalfa selection were conducted in Taiwan. The results showed that some species such as pangola grass (Digitaria decumbens) with good performance and wide adaptability were selected and released. Forage yield and quality of napier grass have been improved and have good results in animal performance. Embryogenic callus cultures from young inflorescences and stem segments of pangola grass could provide an alternative method for rapid propagation and improvement. Three better varieties of alfalfa were selected. They maintain satisfactory stands for two or three years on well-drained sand loam and loam soil, and used only as annual crop in flat area and acid soil. However, more studies in forage breeding for acid soil arc required to maximise the forage quality and animal production. Thus, animal fed with forages of high quality and a few grains supplement will be the future achivement in livestock industry.

High-Performance Spatial and Temporal Error-Concealment Algorithms for Block-Based Video Coding Techniques

  • Hsu, Ching-Ting;Chen, Mei-Juan;Liao, Wen-Wei;Lo, Shen-Yi
    • ETRI Journal
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    • v.27 no.1
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    • pp.53-63
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    • 2005
  • A compressed video bitstream is sensitive to errors that may severely degrade the reconstructed images even when the bit error rate is small. One approach to combat the impact of such errors is the use of error concealment at the decoder without increasing the bit rate or changing the encoder. For spatial-error concealment, we propose a method featuring edge continuity and texture preservation as well as low computation to reconstruct more visually acceptable images. Aiming at temporal error concealment, we propose a two-step algorithm based on block matching principles in which the assumption of smooth and uniform motion for some adjacent blocks is adopted. As simulation results show, the proposed spatial and temporal methods provide better reconstruction quality for damaged images than other methods.

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Analysis of Reducing Characteristics of Direct Reduced Iron using Blast Furnace Dust

  • Yun, Young Min;Chu, Yong Sik;Seo, Sung Kwan;Jeong, Jae Hyun
    • Journal of the Korean Ceramic Society
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    • v.53 no.4
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    • pp.444-449
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    • 2016
  • Industrial by-products generated by integrated iron and steel manufacture cause environmental pollution. The by-products contain not only iron element but also harmful substances. Therefore, in view of to waste recycling and environmental preservation, production of sponge iron using the by-product is considered an effective recycling method. In this study, reduction efficiency of pellets from blast furnace dust was measured. Metallization was found to be increased, as $C/Fe_{total}$ ratio and reaction time were increased. The pellets were formed into a globular shape, and calcined for 60 minutes at $1100^{\circ}C$ in an electric furnace. Phase changes were analyzed using an X-ray diffractometer. Microstructures of the pellets were observed by a scanning electron microscope.

A Study on the Performance Improvement for a Natural Gas Engine under Lean Burn & WOT Condition (전부하시 희박영역에서의 천연가스엔진 성능향상에 관한 연구)

  • 김창업;김창기;김승수;방효선;한정옥;조양수
    • Transactions of the Korean Society of Automotive Engineers
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    • v.4 no.6
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    • pp.11-17
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    • 1996
  • Many researches on natural gas engines, with lean mixtures are being conducted for the purpose of preservation of global environment. Lean combustion is one of the most promising method for increasing engine efficiency and reducing the emission from SI engines. Due to the possibility of partial burn and misfire, however, under lean burn operation, stable flame kernel formation and fast burn rate, by use of swirl or tumble flow, are needed to guarantee a successful subsequent combustion. Experimental data were obtained on a 4-stroke, natural gas fueled SI engine to investigate the effect of compression ratio, swirl and spark plug electrode rotation on efficiency and emission under lean burn condition. Experimental results have displayed that higher compression ratio, presence of swirl vane and favorable direction of electrode gap brougth about the improvements in engine efficiency and its operational stability.

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Repair of Complete Cleft Lip Using Extended Mohler Repair (완전 구순열에서 확장 Mohler법의 적용)

  • Park, Young-Wook
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.34 no.3
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    • pp.200-204
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    • 2012
  • In the repair of unilateral complete cleft lip, the most popular method is the rotation-advancement by Millard. Despite advantages of Millard repair, a few pitfalls exist. Above all, some of the scars, at the height of the cleft side philtral ridge, cross the Langer's line. Further, in the repair of complete cleft lip, small triangular lateral lip flap is often added in the base of an advancement flap to level the Cupid's bow. Moreover, preservation of the advancement flap has some negative effects on a primary nasal repair. As a result, the shape of philtrum is somewhat unnatural. Therefore, I applied the extended Mohler repair in the six cases of complete wide cleft lip to get a more esthetic scar. As a result, more natural, straight philtral ridge was obtained, without adding small triangular flap in the base of the advancement flap.