• Proceedings of the Zoological Society Korea Conference
• /
• 1997.04a
• /
• pp.73.1-73
• /
• 1997

No Abstract, See Full Text

• 대한생식의학회:학술대회논문집
• /
• 2001.11a
• /
• pp.13-22
• /
• 2001

• 대한생식의학회:학술대회논문집
• /
• 2006.06a
• /
• pp.48-49
• /
• 2006

• Clinical and Experimental Reproductive Medicine
• /
• v.27 no.4
• /
• pp.381-385
• /
• 2000

Objective: To verify the effect of two forms (growth factor and growthfactor-reduced) of solubilized Matrigel on the development in mouse preimplantation embryos. Methods: Late 2-cell stage eggs were cultured through the blastocyst stage in the presence of GF- or GFR-Matrigel (0.5%, v/v). Morphological development, cell number and % apoptotic nuclei of blastocyst were measured by Roecst staining and TUNEL of nuclei. Results: Morphological development, number of cells per embryo was significantly increased in the presence of GF- or GFR-Matrigel. Culture of the embryos in the GF-Matrigel gave the best result. Conclusion: Low concentration of solubilized Matrigel improved development of mouse embryos regardless of growth factor content of the Matrigel. Growth factors and extracellular matrix protein included in the Matrigel synergistically potentiated the development of mouse embryos.

• Proceedings of the KSAR Conference
• /
• 2004.06a
• /
• pp.235-235
• /
• 2004

Although studies for important genes concerned in preimplantation stage that encompasses the period from fertilization to implantation were reported for mice and cows, little information relevant to this subject is known in pigs. (omitted)

• Proceedings of the KSAR Conference
• /
• 2004.06a
• /
• pp.237-237
• /
• 2004

Parthenogenesis and culture condition are essential to intracytoplasmic sperm injection and cloning by nuclear transfer. This study investigated apoptosis and in vitro development of parthenogenetic preimplantation porcine embryos. 42∼44 h in vitro matured oocytes derived from a local abattoir were used. Apoptotic cell death was analyzed by using a terminal deoxynucleatidyl transferase mediated deoxyuridine 5-triphoshate nick-end labling (TUNEL) assay. (omitted)

• Korean Journal of Animal Reproduction
• /
• v.19 no.3
• /
• pp.161-169
• /
• 1995

To investigate the metabolism of various substrates in preimplantation bovine embryos, uptake of glucose and pyruvate, and lactate production were measured in single IVF-derived bovine embryos by a non-invasive method. When the embryos were incubated for 5 h in culture medium supplemented with 1 mM glucose and 0.4mM pyruvate as substrates at each developmental stage, glucose uptake was increased with more advanced developmental stages while pyruvate uptake was decreased. Total lactate producton of 2-cell embryos was significantly higher than that of blastocysts (p<0.05). Both of glucose uptake and lactate production in normal morulae produced in vitro was significantly high compared to the degenerated embryos(p<0.05). The results obtained in the study suggest that pyruvate as an exogenous substrate may be support in bovine embryos until 8-cell stage, whereas glucose may be effective as an energy source after morula stage. In addition, it was proven thatlactate was not effective as an energy source in preimplantation development of IVF-derived bovine embryos.

• Clinical and Experimental Reproductive Medicine
• /
• v.51 no.2
• /
• pp.91-101
• /
• 2024

Infertility is a complex disease characterized by extreme genetic heterogeneity, compounded by various environmental factors. While there are exceptions, individual genetic and genomic variations related to infertility are typically rare, often family-specific, and may serve as susceptibility factors rather than direct causes of the disease. Consequently, identifying the cause of infertility and developing prevention and treatment strategies based on these factors remain challenging tasks, even in the modern genomic era. In this review, we first examine the genetic and genomic variations associated with infertility, and subsequently summarize the concepts and methods of preimplantation genetic testing in light of advances in genome analysis technology.

• Proceedings of the Zoological Society Korea Conference
• /
• 2000.04a
• /
• pp.78.1-78
• /
• 2000

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
• /
• 1998.04a
• /
• pp.46.2-46
• /
• 1998

No Abstract, See Full Text