• 한국항해항만학회지
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• 제34권3호
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• pp.175-180
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• 2010

대부분의 응용분야에서 GNSS가 주 측위 시스템으로 활용되고 있으나, 방해전파에 대한 취약성으로 인해 최근에 몇몇 국가에서 eLoran 시스템을 GNSS 백업용으로 사용하기 위한 연구를 진행 중이다. eLoran 시스템의 구축을 위해서는 기존 Loran 시스템에서 설비의 업그레이드, 데이터 채널 사용, dLoran 사이트 추가 구성, 전파 지연오차 보상을 통한 성능 향상이 필요하다. eLoran 신호를 이용한 측위 시에 정확도 성능에 가장 큰 영향을 미치는 오차요소는 육지를 통해 전파될 때 겪는 부가적인 지연요소인 ASF이다. ASF는 지상파 신호가 전파시에 가변적인 고도, 유전율, 도전율 특성을 갖는 육지를 통과하며 발생하는 지연요소이다. 따라서 지상파를 이용한 항법 시에 ASF에 대한 보상모델을 설정하는 것은 매우 중요하다. 본 논문에서는 몬테쓰 모델 (Monteath's Model)을 사용하여 ASF 예측치를 모델링하고, Loran 신호를 이용한 실측을 통해 ASF 실측치를 측정한 후, ASF 예측치와 실측치를 비교하고 특성을 도출하였다. 실험대상 지역은 대전 KRISS와 포항 근방이며, GRI 9930 체인 중 주국인 포항 송신국의 신호를 사용하였다. 실험을 통해 ASF 실측치의 반복성을 확인하고, ASF 예측치와 실측치 간에 일정한 추이를 보이는 것을 확인하였다.

• 한국항해항만학회지
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• 제37권4호
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• pp.375-381
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• 2013

eLoran 시스템의 구축을 위해서는 기존 LORAN-C 설비의 보완과 데이터채널, dLoran 기준국, ASF 데이터베이스 등의 추가가 필요하다. 특히 항만접근 시 eLoran을 이용한 정밀 위치측정을 위해서는 항만 해역에 대한 ASF 맵이 반드시 이용자에게 제공되어야 한다. 본 연구에서는 eLoran 시스템의 주요 오차 요인인 항만에서의 ASF를 효율적으로 생성 및 보완하기 위하여, ASF 예측모델과 실측치를 이용한 ASF 맵 생성기법에 대해 연구하였다. 포항 LORAN-C 주국(9930M)에서 송신신호와 LORAN-C 수신기의 수신신호를 각각 세슘원자시계를 기준으로 측정하는 전파지연 측정법을 적용하여 ASF 실측치를 얻었고, ASF 예측맵은 불규칙한 지형을 적용한 몬테스 모델로 구현하였다. 본 논문에서는 영일만 해상 12 개 측정점에서의 ASF 실측값과 ASF 모델링을 통해 획득한 예측값의 옵셋을 보정하여 영일만의 ASF 맵을 생성하였다.

• 한국항해항만학회지
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• 제35권8호
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• pp.619-624
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• 2011

Loran(LOang RAnge Navigation) 신호를 이용한 측위 시에 정확도에 가장 큰 영향을 미치는 오차요소는 TOA(Time of Arrival) 측정에서의 ASF(Additional Secondary Factor)이다. 따라서 공항접근이나 항만 접안 등의 측위 정확도를 만족시키려면 먼저 정확한 ASF측정이 선행되어야 하는데, 본 연구에서는 해상에서 ASF를 측정하는 기법을 연구하였다. 그 측정방법으로 포항 Loran-C 주국(9930M)에서 송신하는 로란 신호와 로란 수신기의 기준신호를 세슘원자시계를 기준으로 측정함으로써 해상에서의 ASF를 측정하였고 영일만 해상의 12 곳의 측정지점을 3 km 간격으로 설정하여 측정하였다. 해상측정에서 정확도를 높이기 위해서 전기장 안테나와 자기장 안테나를 동시에 사용하였으며 정확한 위치측정을 위해서 DGPS(Differential GPS)수신기를 이용하였다. 이런 방법을 이용하여 해상에서 ASF를 측정함으로써 ASF 예측값과 비교한 결과를 얻었다.

• Journal of Ecology and Environment
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• 제46권1호
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• pp.54-61
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• 2022

Background: In South Korea, African swine fever virus (ASFV) has spread among wild boars through Gangwon-do to Dangyang-gun, Chungcheongbuk-do on the southern border of Gangwon-do. To prevent the spread of ASFV to African swine fever (ASF)-free areas, it is necessary to identify areas with a high probability of finding ASFV-infected carcasses and to reduce the density of wild boars in those areas. In this study, we described the propagation trend of ASFV among wild boars, constructed the habitat suitability maps for ASFV-infected carcasses, and suggested areas with a high probability of finding ASFV-infected carcasses and an important route of ASFV transmission. Results: Despite the active quarantine policies in Korea to prevent the spread of ASFV through wild boars, there was no significant difference in the monthly average of number of ASFV-infected carcasses observed between 2020 and 2021. The ASFV-infected carcasses were found more in winter and spring (January to April). Since the first ASF outbreak in wild boars on October 2, 2019, the maximum width of ASFV-infected carcass distribution area was 222.7 km for about 26 months till November 20, 2021. The habitat suitability map, based on GPS coordinates of ASFV-infected wild boar carcasses, shows that highly detectable areas of ASFV-infected carcasses were sporadically dispersed in western and southwestern parts of Gangwon-do, and ranged from north to south of the province along the Baekdudaegan Mountains, whereas poorly detectable areas ranged along the north to the south in the middle parts of the province. Conclusions: Our suitability model, based on the GPS coordinates of ASFV-infected carcasses, identifies potential habitats where ASFV-infected carcasses are likely to be found and ponential routes where ASFV is likely to spread. Among ASF-free areas, the areas with high suitability predicted in this study should be given priority as survey areas to find ASFV-infected carcasses and hunting areas to reduce wild boar populations.

• Journal of Veterinary Science
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• 제23권4호
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• pp.55.1-55.12
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• 2022

Background: ASF was first reported in Kenya in 1910 in 1921. In China, ASF spread to 31 provinces including Henan and Jiangsu within six months after it was first reported on August 3, 2018. The epidemic almost affected the whole China, causing direct economic losses of tens of billions of yuan. Cause great loss to our pig industry. As ELISA is cheap and easy to operate, OIE regards it as the preferred serological method for ASF detection. P54 protein has good antigenicity and is an ideal antigen for detection. Objective: To identify a conservative site in the African swine fever virus (ASFV) p54 protein and perform a Cloth-enzyme-linked immunosorbent assay (ELISA) for detecting the ASFV antibody in order to reduce risks posed by using the live virus in diagnostic assays. Method: We used bioinformatics methods to predict the antigen epitope of the ASFV p54 protein in combination with the antigenic index and artificially synthesized the predicted antigen epitope peptides. Using ASFV-positive serum and specific monoclonal antibodies (mAbs), we performed indirect ELISA and blocking ELISA to verify the immunological properties of the predicted epitope polypeptide. Results: The results of our prediction revealed that the possible antigen epitope regions were A23-29, A36-45, A72-94, A114-120, A124-130, and A137-150. The indirect ELISA showed that the peptides A23-29, A36-45, A72-94, A114-120, and A137-150 have good antigenicity. Moreover, the A36-45 polypeptide can react specifically with the mAb secreted by hybridoma cells, and its binding site contains a minimum number of essential amino acids in the sequence 37DIQFINPY44. Conclusions: Our study confirmed a conservative antigenic site in the ASFV p54 protein and its amino acid sequence. A competitive ELISA method for detecting ASFV antibodies was established based on recombinant p54 and matching mAb. Moreover, testing the protein sequence alignment verified that the method can theoretically detect antibodies produced by pigs affected by nearly all ASFVs worldwide.

• Molecules and Cells
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• 제37권1호
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• pp.81-87
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• 2014

Chronic pressure-overload cardiac hypertrophy is associated with an increased risk of morbidity/mortality, largely due to maladaptive remodeling and dilatation that progresses to dilated cardiomyopathy. Alternative splicing is an important biological mechanism that generates proteomic complexity and diversity. The recent development of next-generation RNA sequencing has improved our understanding of the qualitative signatures associated with alternative splicing in various biological conditions. However, the role of alternative splicing in cardiac hypertrophy is yet unknown. The present study employed RNA-Seq and a bioinformatic approach to detect the RNA splicing regulatory elements involved in alternative splicing during pressure-overload cardiac hypertrophy. We found GC-rich exonic motifs that regulate intron retention in 5' UTRs and AT-rich exonic motifs that are involved in exclusion of the AT-rich elements that cause mRNA instability in 3' UTRs. We also identified motifs in the intronic regions involved in exon exclusion and inclusion, which predicted splicing factors that bind to these motifs. We found, through Western blotting, that the expression levels of three splicing factors, ESRP1, PTB and SF2/ASF, were significantly altered during cardiac hypertrophy. Collectively, the present results suggest that chronic pressure-overload hypertrophy is closely associated with distinct alternative splicing due to altered expression of splicing factors.