• Journal of Pharmacopuncture
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• v.17 no.4
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• pp.36-49
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• 2014

Objectives: Quercetin and curcuminoids are important bioactive compounds found in many herbs. Previously reported high performance liquid chromatography ultraviolet (HPLC-UV) methods for the detection of quercetin and curcuminoids have several disadvantages, including unsatisfactory separation times and lack of validation according the standard guidelines of the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. Methods: A rapid, specific, reversed phase, HPLC-UV method with an isocratic elution of acetonitrile and 2% v/v acetic acid (40% : 60% v/v) (pH 2.6) at a flow rate of 1.3 mL/minutes, a column temperature of $35^{\circ}C$, and ultraviolet (UV) detection at 370 nm was developed. The method was validated and applied to the quantification of different types of market available Chinese medicine extracts, pills and tablets. Results: The method allowed simultaneous determination of quercetin, bisdemethoxycurcumin, demethoxycurcumin and curcumin in the concentration ranges of $0.00488-200{\mu}g/mL$, $0.625-320{\mu}g/mL$, $0.07813-320{\mu}g/mL$ and $0.03906-320{\mu}g/mL$, respectively. The limits of detection and quantification, respectively, were 0.00488 and $0.03906{\mu}g/mL$ for quercetin, 0.62500 and $2.50000{\mu}g/mL$ for bisdemethoxycurcumin, 0.07813 and $0.31250{\mu}g/mL$ for demethoxycurcumin, and 0.03906 and $0.07813{\mu}g/mL$ for curcumin. The percent relative intra day standard deviation (% RSD) values were $0.432-0.806{\mu}g/mL$, $0.576-0.723{\mu}g/mL$, $0.635-0.752{\mu}g/mL$ and $0.655-0.732{\mu}g/mL$ for quercetin, bisdemethoxycurcumin, demethoxycurcumin and curcumin, respectively, and those for intra day precision were $0.323-0.968{\mu}g/mL$, $0.805-0.854{\mu}g/mL$, $0.078-0.844{\mu}g/mL$ and $0.275-0.829{\mu}g/mL$, respectively. The intra day accuracies were 99.589%-100.821%, 98.588%-101.084%, 9.289%-100.88%, and 98.292%-101.022% for quercetin, bisdemethoxycurcumin, demethoxycurcumin and curcumin, respectively, and the inter day accuracy were 99.665%-103.06%, 97.669%-103.513%, 99.569%-103.617%, and 97.929%-103.606%, respectively. Conclusion: The method was found to be simple, accurate and precise and is recommended for routine quality control analysis of commercial Chinese medicine products containing the flour flavonoids as their principle components in the extracts.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.40 no.11
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• pp.2291-2297
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• 2015

This paper presents a vehicle detection system using the video as a input image what has moving of vehicles.. Input image has constraints. it has to get fixed view and downward view obliquely from top of the road. Road detection is required to use only the road area in the input image. In introduction, we suggest the experiment result and the critical point of motion history image extraction method, SIFT(Scale_Invariant Feature Transform) algorithm and histogram analysis to detect vehicles. To solve these problem, we propose using applied Gaussian Mixture Model(GMM) that is the Vehicle Detection GMM(VDGMM). In addition, we optimize VDGMM to detect vehicles more and named VDGMM 2.0. In result of experiment, each precision, recall and F1 rate is 9%, 53%, 15% for GMM without road detection and 85%, 77%, 80% for VDGMM2.0 with road detection.

• KSBB Journal
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• v.21 no.5
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• pp.325-330
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• 2006

This study presents the characterization of an integrated portable microfluidic electrical detection system for fast and low volume immunoassay using polystyrene microbead, which are used as immobilization surfaces. In our chip, a filtration method using the microbead was adopted for sample immobilization and immunogold silver staining(IGSS) was used to increase the electrical signal. The chip is composed of an inexpensive and biocompatible Polydimethylsiloxane(PDMS) layer and Pyrex glass substrate. Platinum microelectrodes for electric signal detection were fabricated on the substrate and microchannel and pillar-type microfilters were formed in the PDMS layer. With a fabricated chip, we reacted antigen and antibody according to the procedures. Then, silver enhancer was injected to increase the size of nanogold particles tagged with the second antibody. As a result, microbeads were connected to each other and formed an electrical bridge between microelectrodes. Resistance measured through the electrodes showed a difference of two orders of magnitude between specific and nonspecific immuno-reactions. The detection limit was 10 ng/ml. The developed immunoassay chip reduced the total analysis time from 3 hours to 50 min. Fast and low-volume biochemical analysis has been successfully achieved with the developed microfilter and immuno-sensor chip, which is integrated to the microfluidic system.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.5
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• pp.704-711
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• 2020

Objective: Muscle fiber types, numbers and area are crucial aspects associated with meat production and quality. However, there are few studies of pig muscle fibre traits in terms of the detection power, false discovery rate and confidence interval precision of whole-genome quantitative trait loci (QTL). We had previously performed genome scanning for muscle fibre traits using 183 microsatellites and detected 8 significant QTLs in a White Duroc×Erhualian F₂ population. The confidence intervals of these QTLs ranged between 11 and 127 centimorgan (cM), which contained hundreds of genes and hampered the identification of QTLs. A whole-genome sequence imputation of the population was used for fine mapping in this study. Methods: A whole-genome sequences association study was performed in the F₂ population. Genotyping was performed for 1,020 individuals (19 F₀, 68 F₁, and 933 F₂). The whole-genome variants were imputed and 21,624,800 single nucleotide polymorphisms (SNPs) were identified and examined for associations to 11 longissimus dorsi muscle fiber traits. Results: A total of 3,201 significant SNPs comprising 7 novel QTLs showing associations with the relative area of fiber type I (I_RA), the fiber number per square centimeter (FN) and the total fiber number (TFN). Moreover, one QTL on pig chromosome 14 was found to affect both FN and TFN. Furthermore, four plausible candidate genes associated with FN (kinase non-catalytic C-lobe domain containing [KNDC1]), TFN (KNDC1), and I_RA (solute carrier family 36 member 4, contactin associated protein like 5, and glutamate metabotropic receptor 8) were identified. Conclusion: An efficient and powerful imputation-based association approach was utilized to identify genes potentially associated with muscle fiber traits. These identified genes and SNPs could be explored to improve meat production and quality via marker-assisted selection in pigs.

• Journal of the Korean Society of Industry Convergence
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• v.20 no.5
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• pp.395-404
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• 2017

Cultivating soybeans in rice paddy field reduces labor costs and increases the yield. Soybeans, however, are highly susceptible to excessive soil water in paddy field. Controlled drainage system can adjust groundwater level (GWL) and control soil moisture content, resulting in improvement soil environments for optimum crop growth. The objective of this study was to fit the soybean growth data (canopy height and stem diameter) using Gompertz model and Logistic model at different GWL and validate those models. The soybean, Daewon cultivar, was grown on the lysimeters controlled GWL (20cm and 40cm). The soil textures were silt loam and sandy loam. The canopy height and stem diameter were measured from the 20th days after seeding until harvest. The Gompertz and Logistic models were fitted with the growth data and each growth rate and maximum growth value was estimated. At the canopy height, the $R_2$ and RMSE were 0.99 and 1.58 in Gompertz model and 0.99 and 1.33 in Logistic model, respectively. The large discrepancy was shown in full maturity stage (R8), where plants have shed substantial amount of leaves. Regardless of soil texture, the maximum growth values at 40cm GWL were greater than the value at 20cm GWL. The growth rates were larger at silt loam. At the stem diameter, the $R_2$ and RMSE were 0.96 and 0.27 in Gompertz model and 0.96 and 0.26 in Logistic model, respectively. Unlike the canopy height, the stem diameter in R8 stage didn't decrease significantly. At both GWLs, the maximum growth values and the growth rates at silt loam were all larger than the values at sandy loam. In conclusion, Gompertz model and Logistic model both well fit the canopy heights and stem diameters of soybeans. These growth models can provide invaluable information for the development of precision water management system.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.30 no.2
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• pp.95-100
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• 2019

Background and Objectives For patients with polyps and cysts, glottal gaps resulting from their lesions have negative respiratory effects when they vocalize. Phonatory Aerodynamic System is clinically used, but is often limited in the measurement of vowels. So the researchers attempted to verify the usefulness of Phonatory Aerodynamic System by comparing differences in respiratory characteristics and patterns which can be measured by the level of connected speech. Materials and Method Among the subjects who were diagnosed through a stroboscopy, there were 33 patients with polyps and 23 patients with cysts. Then, 36 subjects who were found to have no specific findings through a stroboscopy and perceptual test were selected to the normal group. We compared respiratory characteristics and patterns. And compared vocal polyps and cysts before and after laryngeal micro surgery (LMS). Results First, difference in respiratory patterns between the normal group and the patients with polyps and cysts were examined to show that breath groups, breath group syllables, and expiratory·inspiratory volume were significantly higher in the polyp/cyst group than those in the normal group, indicating that precision was lowered during the conversation, due to reduction in speech intelligibility and interruption of communication. Second, there were significant differences in maximum phonation time, mean flow rate, and subglottal pressure among respiratory characteristics, breath groups, breath group syllables, and inspiratory volume before and after LMS, which appeared to be similar to the normal group. Conclusion The understanding of respiratory characteristics and patterns produced by patients in connected speech which is most similar to natural speech was found to be the objective and useful method for examining characteristics of the subjects.

• Korean Journal of Food Science and Technology
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• v.38 no.6
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• pp.725-729
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• 2006

A method was developed for the determination of fructooligosaccharides and raffinose contents in infant formula. The samples were extracted and analyzed by liquid chromatography equipped with carbohydrate column and evaporative light scattering detector. The mobile phase used for the gradient mode was water-acetonitrile, at a flow rate of 1.0mL/min. The method showed a mean recovery of 95-99%, the relative standard deviation obtained in the precision study was 0.774-8.982%, the quantification and detection limits were 25-50mg/L.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.33 no.2
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• pp.83-94
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• 2015

Recently, the necessity of spatial data in unaccessible area was challenged to set up various plans and policies for preparing the unification and the cooperative projects between South-North Korea. Therefore, this paper planned to evaluate the possibility of adopting the error tolerance in Geometric correction for 1/5,000 digital topographic mapping, using the PLEIADES images and the TerraSAR GCPs (Ground Control Points). The geometric correction was performed by changing the number and placement of GCPs by GPS (Global Positioning System) surveying, as the optimal placement of 5 GCPs were selected considering the geometric stability and steady rate. The positional accuracy evaluated by the TerraSAR GCPs, which were selected by optimal placement of GCPs. The RMSE in control points were X=±0.64m, Y=±0.46m, Z=±0.28m. While the result of geometric correction for PLEIADES images confirmed that the RMSE in control points were X=±0.34m, Y=±0.27m, Z=±0.11m, the RMSE in check points were X=±0.50m, Y=±0.30m, Z=±0.66m. Through this study, we believe if spatial data can integrate with the PLEIADES images and the optimal TerraSAR GCPs, it will be able to obtain the high-precision spatial data for adopting the regulation of 1/5,000 digital topographic map, which adjusts the computation as well as the error bound.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.35 no.6
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• pp.609-616
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• 2017

National Geographic Information Institute has established a plan that preoccupies UCPs (Unified Control Points) at 2~3km intervals in urban areas by considering the distance between existing UCPs by satellite images and aerial photographs in 2015. In this study, we discussed the method of selecting the locations of optimal UCPs by simulating GPS reception environment in candidate sites for UCPs using GIS. For this purpose, we selected new candidate sites for installing UCPs using satellite images and aerial photographs, and analyzed the GPS reception environment by calculating the visibility distance from buildings around UCPs using GIS skyline analysis. The number of and the arrangement of visible satellites that are capable of GPS satellite reception from the viewpoint of sky view were showed by GIS skyline analysis. Quality evaluation results of GPS observation data were compared with average PDOP calculated from hourly PDOP and TEQC in two points of Sungkyunkwan University during 8 hours. As a result of GPS reception environment using GIS, if the PDOP increases, the data acquisition rate is lowed, and the multipath error and the cycle slip are increased. Thus, this study verified that the quality of GPS observation data can be secured by constructing three-dimensional spatial information and simulating PDOP when preoccupying multiple UCPs using GIS.

• 한국해양학회지
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• v.27 no.1
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• pp.19-34
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• 1992

Marine seismic refraction surveys have been carried out by Korea Institute of Geology, Mining and Materials(KIGAM) since 1984. The recording of refraction data was based on analog instrumentation. Therefore the resolution of refraction data was not good enough to distinguish many layers. The objective of the interpretation of seismic refraction data is the determination of intervals and critically refracted seismic wave propagation velocities through the layers beneath the sea floor. To determine intervals and velocities precisely, the resolution of refraction data should be enhanced. The intent of the study is to improve the quality of shallow marine refraction data by the digital technique using microcomputer- based acquisition and processing system. The system consists of an IBM AT microcomputer clone, an analog-digital(A/D) converter. A mass storage unit and a parallel processing board. The A/D converter has 12 bits of precision and 250 kHz of conversion rate. The magneto-optical disk drive is used for the mass storage of seismic refraction data. Shallow marine seismic refraction surveys have been carried out using the system at 6 locations off Ulsan and Pusan area. The refraction data were acquired by the radio sonobuoy. The refraction profiles have been produced by the laser printer with 300 dpi resolution after the basic computer processing. 5-9 layers were interpreted from digital refraction profiles, whereas 2-4 layers were interpreted from analog refraction profiles. the propagation velocities of sediments were interpreted as 1.6-2.1 km/sec. The propagation velocities of acoustic basement were interpreted as 2.4-2.7 km/sec off Ulsan area, 4.8 km/sec off Pusan area.