• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.215-222
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• 2006

The Lowry method was used in this study to measure protein in Haemophilus influenzae type b (Hib) conjugate vaccines (polyribosylibitol phosphate-tetanus toxoid; PRP-TT) using deoxycholic acid (DOC) to induce protein precipitation. Trichloroacetic acid (TCA) did not induce precipitation adequately from the Hib conjugate bulk and the freeze-dried Hib conjugate product. Its yield was approximately 50%. The matrix structure of Hib conjugate inhibits precipitation by TCA. Although the Lowry method can be carried out without precipitation in Hib conjugate bulk when no residual impurities (adipic acid dihydrazide [ADH], 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide-HCI [EDAC], phenol and cyanogens bromide [CNBr], etc.) are present, it cannot be used for Hib conjugate products that contain sucrose 8.5%, because 8.5% concentration of sucrose enhanced the protein concentration. DOC- and HCl-induced precipitation is an alternative method for evaluating the protein content of the Hib conjugate bulk and the Hib conjugate product. The precipitation was optimal with a final concentrate of 0.1% for DOC at $4^{\circ}C$ and pH 2. This Lowry method, using DOC/HCI precipitation to induce protein precipitation, was confirmed a consistent, reproducible, and valid test for proteins in Hib conjugate bulk and its freeze-dried product.

• 한국환경과학회지
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• 제27권12호
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• pp.1227-1239
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• 2018

In this study, daily precipitation data and daily average temperature data of meteorological observatories in Daegu, Busan, Daejeon, Seoul, Mokpo, and Gwangju cities inland and offshore were analyzed by using moving average method. Were compared. Overall, summarizing changes in precipitation and temperature over the 24 seasons, precipitation and temperature in all six stations increased compared to the past 1960s. In the case of precipitation, precipitation increased at the end of July and early August, whereas precipitation in April, September and early October decreased. In the case of temperature, especially in February, the temperature increased, and in Mokpo, the temperature from August to December showed a general decline. Changes in precipitation and temperature due to seasons in the 24 seasons affect agriculture and our everyday life, and further research is needed to determine how these changes will affect agricultural water supply, crop growth and daily life. The results of this study can be useful.

• 대한토목학회논문집
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• 제40권3호
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• pp.265-271
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• 2020

첨단 차량에서 가장 중요한 장비 중 하나인 차량레이더는 일반적으로 자동차와 같은 객체의 속도와 범위를 감지하는데 사용된다. 본 논문에서는 객체감지 이외에 차량레이더 자료를 이용하여 강수 정보를 추정하는 방법을 제안한다. 제안된 방법은 레이더 수신신호의 감쇠 정도가 강수 강도에 따라 다르다는 사실과 시공간적으로 짧은 관측에서는 강수의 분포가 일정하다는 가정에 근거한다. 본 논문은 차량 레이더를 이용하여 강수정보 추정에 대한 타당성을 평가하는데 목적이 있다. 실제 주행 중 제안 된 방법의 실현 가능성을 검증하기 위해, 다양한 강수 사상의 각 시간 세그먼트에 대한 강수정보 추정 방법이 적용되었다. 주행 현장실험의 결과로부터 제안된 방법이 다양한 강우 유형에서 강수 정보 추정에 적합하다는 것을 알 수 있었다.

• 한국수자원학회논문집
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• 제55권12호
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• pp.1053-1064
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• 2022

현행 면적평균 강수량 산정 방법인 티센 방법은 정확한 유역평균 강수량 산정에 있어 심각한 구조적 한계가 존재한다. 강수량계의 관측 정확도 외에, 강수량계 배치와 호우의 이동 방향에 따라서도 면적평균 강수량 산정에 오차가 발생할 수 있다. 유역이 작고 관측소 밀도가 희박한 경우 시뮬레이션 및 관측 사상 모두에서 티센 방법은 첨두 전후로 10분 사이에 유역평균 강수량이 계속 급격히 증감이 반복되는 특이한 경향 보였다. 그리고 티센 유역평균 강수량은 첨두 시점이 강우레이더와 다르게 나타났다. 유역이 작지만 관측소 밀도 비교적 높은 경우에는 전반적으로 티센 방법에 의해 톱니모양의 과대 첨두치의 경향은 나타나지 않았고 시간에 따른 변동이 유사하게 나타났다. 그러나 강우레이더 관측치와 지상 강수량계 관측치 유역평균 강수량 사이에 약 10분 정도의 연속적인 시차가 발생하였다. 강우레이더 유역평균 강수량의 지상보정 효과를 검토한 결과, 보정 후 면적평균 강수량이 보정 전 면적평균 강수량에 비해 오히려 상관이 낮게 나타나, 현행 강우레이더 지상보정 알고리즘 보정 효과가 높지 않은 것을 알 수 있었다.

• 한국수자원학회논문집
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• 제31권6호
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• pp.657-665
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• 1998

홍수가 발생하기 전에 위험을 진단할 수 있는 기법이 다음의 절차를 따라 마련되었다. 현존 가용 수자원이 평균치 보다 많은 지 여부를 결정한다. 평균치보다 많은 날의 연속기간을 계산한다. 연속된 기간동안 강수량을 누적한다. 누적된 강수량 중에서 일별 감소량(유출 및 증발산등에 의한)을 감한 다음 다시 하루강수량으로 환산한 것이 유효강수량이다. 유효강수량을 다시 계절적, 지역적 평균치와 비교하여 일반화된 수자원의 집중정도 즉 유효강수지수를 구한다. 유효강수지수가 큰 값을 가지면 홍수의 위험은 증대한다. 이 방법은 1996년 7월말 한반도의 경기 강원지역의 홍수사례를 분석한 연구(한국수자원학회, 1996)와 동일한 사례에 적용하여 그 결과를 비교하였다. 종래의 강우-유출 모형이 가지는 두 가지 과정, 즉 매개변수 추정의 단계와 수위관측의 단계를 생략하였기 때문에 계산이 신속하게 되어 위급할 때 사용될 수 있는 장점이 확인되었다. 두 과정을 생략하고도 유출량이 고려되었기 때문에 강수량만으로 진단하는 방법보다 과학적임이 입증되었다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2023년도 학술발표회
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• pp.180-180
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• 2023

To improve global risk management, understanding the characteristics and distribution of precipitation is crucial. However, obtaining spatially and temporally resolved climatic data remains challenging due to sparse gauge observations and limited data availability, despite the use of satellite and reanalysis products. To address this challenge, merging available precipitation products has been introduced to generate spatially and temporally reliable data by taking advantage of the strength of the individual products. However, most of the existing studies utilize all the available products without considering the varying performances of each dataset in different regions. Comprehensively considering the relative contributions of each parent dataset is necessary since their contributions may vary significantly and utilizing all the available datasets for data merging may lead to significant data redundancy issues. Hence, for this study, we introduce a site-specific precipitation merging method that utilizes the Quadruple Collocation (QC) approach, which acknowledges the existence of error-cross correlation between the parent datasets, to create a high-resolution global daily precipitation data from 2001-2020. The performance of multiple gridded precipitation products are first evaluated per region to determine the best combination of quadruplets to be utilized in estimating the error variances through the QC approach and computation of merging weights. The merged precipitation is then computed by adding the precipitation from each dataset in the quadruplet multiplied by each respective merging weight. Our results show that our approach holds promise for generating reliable global precipitation data for data-scarce regions lacking spatially and temporally resolved precipitation data.

• 한국농공학회지
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• 제23권2호
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• pp.45-53
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• 1981

Long term precipitation gaging station record (58 years) was analyzed by progressive mean method to compare the estimated effective period of records for computing mean and probable values. Obtained results are as follows: 1. Fifty-eight years precipitation records at Jinju, Gyeong Sang Nam Do was analyzed by double mass analysis method. Result was appeared that the record was consistent with time. 2. The effective period of records for estimating mean values with the departure of 5% or less from the true mean are up to 33 years for annual precipitation, 20 years for annual maximum daily precipitation and 45 years for maximum successive dry days in summer season. 3. To estimate the probable values by Gumbel-Chow method within the departure of 5% level from true value, periods of 51 years, 38 years and 45 years were required for annual precipitation, annual maximum daily precipitation and maximum successive dry days in summer season, respectively.

• 환경생물
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• 제23권4호
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• pp.374-378
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• 2005

A diffusion - precipitation method was developed to determine acid volatile sulfide (AVS) concentrations in freshwater sediments. This method uses silver nitrate as a sulfide trap solution and the concentration of trapped sulfide is determined gravimetrically. The proposed diffusion - precipitation method is more rapid and less expensive than previously developed purge- and - trap methods. Spiked sodium sulfide recoveries using this method $(97\~120\%)$ were similar with a previously developed diffusion - absorption method $(93.8\~115\%)$ and about $20\%$ greater than a previously developed purge-and-trap method $(74.6\~105\%)$. Detection limit of this method $(0.1\;{\mu}mole\;S\;g^{-l})$ was comparable with that of diffusion-absorption method $(0.06\;{\mu}mole\;S\;g^{-l})$ and purge-and-trap method $(0.05\~0.5\;{\mu}mole\;S\;g^{-l})$.

• 한국환경과학회지
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• 제28권10호
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• pp.841-849
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• 2019

The purpose of this study was to evaluate the method of estimating the areal precipitation reflecting the altitude of the mountainous terrain on Jeju Island by comparing and analyzing the areal precipitation using the Thiessen polygon method and the isohyetal method in mountainous streams. In terms of constructing the Thiessen polygon network, rainfall errors occurred in 94.5% and 45.8% of the Thiessen area ratio of the Jeju and Ara stations, respectively. This resulted in large areal precipitation and errors using the isohyetal method at altitudes below 600 m in the target watershed. In contrast, there were small errors in the highlands. Rainfall errors occurred in 18.91% of the Thiessen area ratio of Eorimok, 2.41% of Witseoreum, and 2.84% of Azalea Field because of the altitudinal influence of stations located in the highlands at altitudes above 600 m. Based on the areal precipitation estimation using the Thiessen polygon method, it was considered to be partially applicable to streams on Jeju Island depending on the altitude. However, the method is not suitable for mountainous streams such as the streams on Jeju Island because errors occur with altitude. Therefore, the isohyetal method is considered to be more suitable as it considers the locations of the rainfall stations and the orographic effect and because there are no errors with altitude.

• 한국농공학회논문집
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• 제54권5호
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• pp.141-153
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• 2012

In this study, we estimated probable precipitation amounts at the target year (2020, 2030, 2040) of 55 weather stations in Korea using the 24 hour annual maximum precipitation data from 1973 through 2009 which should be useful for management of agricultural reservoirs. Not only trend tests but also non-stationary tests were performed and non-stationary frequency analysis were conducted to all of 55 sites. Gumbel distribution was chosen and probability weighted moment method was used to estimate model parameters. The behavior of the mean of extreme precipitation data, scale parameter, and location parameter were analyzed. The probable precipitation amount at the target year was estimated by a non-stationary frequency analysis using the linear regression analysis for the mean of extreme precipitation data, scale parameter, and location parameter. Overall results demonstrated that the probable precipitation amounts using the non-stationary frequency analysis were overestimated. There were large increase of the probable precipitation amounts of middle part of Korea and decrease at several sites in Southern part. The non-stationary frequency analysis using a linear model should be applicable to relatively short projection periods.