• Journal of Korean Elementary Science Education
• /
• v.32 no.1
• /
• pp.82-94
• /
• 2013

The purpose of this study was to investigate the process and the effect of pre-service teacher's scaffolding in environmental camp program about global climate change. For this study, developed the environmental camp program based pre-service teacher's scaffolding and applied to 78 $5^{th}$ students. We analyzed the role of pre-service teacher in the process of scaffolding. In the result, the pre-service teachers conducted cognitive scaffolding like as "Focus", "Hint", "Tell or Summarize" and "Technical Help". They carried out the emotional scaffolding like as "Create Cheerful Atmosphere", "Encourage", and "Help in Living". Teaching and learning about global climate change, the theme of the camp, was regarded uncertain and complex. So, pre-service teacher's scaffolding was effective to promote environmental literacy about climate change of primary students (<0.05). The student teachers understanded the characteristics of the children through emotionally close relationships. The primary students were learned easier about global climate change through cognitive and emotional scaffolding. They experienced environmental practice with communal living in camp.

• Korean Journal of Microbiology
• /
• v.28 no.1
• /
• pp.13-18
• /
• 1990

A DNA sequence encoding the adr subtype preS2 region of hepatitis B virus envelope protein was fused to 5' end of lacZ gene yielding a plasmid pTSZ, in order to produce a preS2-$\beta$-galactosidase fusion protein. Serial deletions from 3' and 5' end of preS2 were constructed in plasmids, which were expressed and their antigenicities were examined with the monoclonal antibody H8. Deletions from amino and carboxy terminal to certain points did not affect the antigenicity, but the longer deletions destroyed the antigenicity. End points of deleted preS2 sequence were determined by DNA sequencing. As a result, each end of preS2 epitope was located in the region of amino acid residue 130-132 and 140-142, respectively. Residue 143 may be supplementary for antigenic epitope since the deletion from carboxy terminal to residue 143 revealed partial defect of antigenicity. In the interval of antigenic epitope the amino acid differences between adr and adw2 subtype occurred ar residue 130, 132, and 141. This result indicated that one or more of the three residues are responsible for the binding specificity of monoclonal antibody H8 to adr subtype preS2 fusion protein.

• Textile Coloration and Finishing
• /
• v.35 no.1
• /
• pp.1-7
• /
• 2023

In this study, the influence of pre-treatment(bleaching, mercerization by liquid ammonia and caustic soda) on cotton fabrics were investigated on dyeing Rubia cordifolia extracts. Aluminium Sulfate, Iron(Ⅱ) Sulfate Heptahydrate, Copper(Ⅱ) Sulfate Pentahydrate, Tin(Ⅱ) Sulfate, Manganese(Ⅱ) Chloride Tetrahydrate were used as mordanting agents. K/S value and washing fastnesses of the dyed cotton fabrics pretreated under different conditions were investigated. The K/S values were increased in the order of bleaching, liquid ammonia and mercerization pre-treatment. It was found that the pre-treatment conditions did not significantly affect the color change. The colorfastness to washing of most of all dyed fabrics were over grade 4 regardless of pre-treatment condition.

• International Journal of Fluid Machinery and Systems
• /
• v.1 no.1
• /
• pp.10-23
• /
• 2008

The development of pre-set wavelength G$\ddot{o}$rtler vortices are studied in the boundary-layer flows on concave surfaces of 1.0 and 2.0 m radius of curvature. The wavelengths of the vortices were pre-set by thin wires of 0.2 mm diameter placed 10 mm upstream and perpendicular to the concave surface leading edge. Velocity contours were obtained from velocity measurements using a single hot-wire anemometer probe. The most amplified or dominant wavelength is found to be 15 mm for free-stream velocity of 2.1 m/s and 3.0 m/s on the concave surface of R = 1 m and 2 m, respectively. The velocity contours in the cross-sectional planes at several streamwise locations show the growth and breakdown of the vortices. Three different regions can be identified based on the growth rate of the vortices. The occurrence of a secondary instability mode is also shown in the form of mushroom-like structures as a consequence of the non-linear growth of the G$\ddot{o}$rtler vortices. By pre-setting the vortex wavelength to be much larger and much smaller than the most amplified one, the splitting and merging of G$\ddot{o}$rtler vortices can be respectively observed.

• The Korean Journal of Community Living Science
• /
• v.28 no.1
• /
• pp.115-130
• /
• 2017

This study was conducted to examine the relationship between pre-service early childhood teacher's motivations of choosing a teaching profession, educational belief, and self-directed learning readiness. The sample included 308 early childhood education major students, and the data were collected using the Modified Orientation to Teach Survey (MOTS), Teaching-belief type scale, and self-directed learning readiness scale. A statistical analysis included correlation analysis and stepwise multiple regression analysis. The results were as follows: 1) analysis of the relationship between pre-service early childhood teacher's motivations of choosing a teaching profession, educational belief, and self-directed learning readiness conveys that intellectual stimulation and self-directed learning had the strongest relationships while nature of work had the weakest. For educational belief and self-directed learning readiness, maturationism and interactionism showed significantly positive correlations while behaviorism displayed a negative correlation. Behaviorism had a significantly negative correlation with openness for challenge, a sub-factor of self-directed learning. 2) Analysis of the effect of pre-service early childhood teacher's motivations of choosing a teaching profession and educational belief on self-directed learning readiness indicates that pre-service early childhood teacher's motivations of choosing a teaching profession had a stronger effect on self-directed learning. These results suggest the following: successful performance as an early childhood teacher not only requires receiving institutionalized education but also self-directed learning while working as an early childhood teacher.

• Journal of information and communication convergence engineering
• /
• v.19 no.1
• /
• pp.29-35
• /
• 2021

While the population of pet dogs and pet-related markets are increasing, there is no convenient and reliable tool for pet health monitoring for pet owners/caregivers. In this paper, we propose a mobile platform-based pre-diagnosis system that pet owners can use for pre-diagnosis and obtaining information on coping strategies based on their observations of the pet dog's abnormal behavior. The proposed system constructs symptom-disease association databases for 100 frequently observed diseases under veterinarian guidance. Then, we apply the possibilistic fuzzy C-means algorithm to form the "probable disease" set and the "doubtable disease" set from the database. In the experiment, we found that the proposed system found almost all diseases correctly, with an average of 4.5 input symptoms and outputs 1.5 probable and one doubtable disease on average. The utility of this system is to alert the owner's attention to the pet dog's abnormal behavior and obtain an appropriate coping strategy before consult a veterinarian.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2023.05a
• /
• pp.158-158
• /
• 2023

Characterizing the performance of precipitation (hereafter PRE) products in estimating the uncertainties in daily PRE in the era of global warming is of great value to the ecosystem's sustainability and human survival. This study intercompares the performance of different PRE products (gauge-based, satellite and reanalysis) sourced from the Frequent Rainfall Observations on GridS (FROGS) database over diverse climate zones in Africa and identifies regions where they depict minimal uncertainties in order to build optimal maps as a guide for different climate users. This is achieved by utilizing various techniques, including the triple collection (TC) approach, to assess the capabilities and limitations of different PRE products over nine climatic zones over the continent. For daily scale analysis, the uncertainties in light PRE (0.1 5mm/day) are prevalent over most regions in Africa during the study duration (2001-2016). Estimating the occurrence of extreme PRE events based on daily PRE 90th percentile suggests that extreme PRE is mainly detected over central Africa (CAF) region and some coastal regions of west Africa (WAF) where the majority of uncorrected satellite products show good agreement. The detection of PRE days and non-PRE days based on categorical statistics suggests that a perfect POD/FAR score is unattainable irrespective of the product type. Daily PRE uncertainties determined based on quantitative metrics show that consistent, satisfactory performance is demonstrated by the IMERG products (uncorrected), ARCv2, CHIRPSv2, 3B42v7.0 and PERSIANN_CDRv1r1 (corrected), and GPCC, CPC_v1.0, and REGEN_ALL (gauge) during the study period. The optimal maps that show the classification of products in regions where they depict reliable performance can be recommended for various usage for different stakeholders.

• BMB Reports
• /
• v.40 no.6
• /
• pp.1002-1008
• /
• 2007

PreS domain of Hepatitis B virus (HBV) surface antigen is a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes. This report deals with overexpression and purification of adr subtype of surface antigen that is more prevalent in Pakistan. PreS region, comprising 119 aa preS1 region plus a 55 aa preS2 region plus 11 aa from the N-terminal S region, was inserted in pET21a+ vector, cloned in E. coli $DH5\alpha$ cells and expressed in E. coli BL21 codon+ cells. The conditions for over expression were optimized using different concentrations of IPTG (0.01-5 mM), and incubating the cells at different temperatures (23-$41^{\circ}C$) for different durations (0-6 h). The cells were grown under the given optimized conditions (0.5 mM IPTG concentration at $37^{\circ}C$ for 4 h), lysed by sonication and the protein was purified by ion exchange chromatography. On the average, 24.5 mg of recombinant protein was purified per liter of culture. The purified protein was later lyophilized and stored at $-80^{\circ}C$.

• The Korean Journal of Community Living Science
• /
• v.16 no.1
• /
• pp.65-72
• /
• 2005

To increase dyeability of cotton fabric to the chestnut shell dyeing, we had several dyeing experiments with the experimental fabrics which was pre-treated by soybean, NaOH, KOH. Three kinds of experimental cotton fabrics were used and chestnut shell dyebath was made for the experiment. Munsell's color HV/C and color differences ΔE of dyeing fabrics were compared between pre-treatment and none pre-treatment dyeing fabrics. The main results were as follows ; Color differences of dyeing fabrics pre-treated with soybean, NaOH, KOH were higher than those of none pre-treated dyeing fabrics. In case of pre-treatment with soybean, the concentration of the soybean bath affected the color differences but not affected in case of pre-treatment with NaOH & KOH. Munsell's color of pre-treatment fabrics with soybean, NaOH, KOH were same with the none pre-treatment fabrics. Colorfastness of the pre-treated fabrics were same degree, but only in case of soybean pre-treated fabrics were low in fastness of washing(change) & perspiration(acidic change).

• YAKHAK HOEJI
• /
• v.45 no.6
• /
• pp.708-714
• /
• 2001

Many studies have provided evidences that hepatitis B surface antigen (HBsAg) including preS region could be an ideal candidate for a new hepatitis B virus (HBV) vaccine with higher efficacy. We established CHO cell lines, IY-CHO-2 and IY-CHO-11 expressing high levels of HBsAg containing preS2 and S protein by stable transfection method. These cell lines expressed the correct size (about 1 kb in length) of HBsAg mRNA as expected. The purified protein from the culture supernatants of the clones showed the same sizes as those expressed in native hepatitis B virus (24 kDa, 27 kDa, 34 kDa and 36 kDa). Antibody productivity of CHO-derived HBsAg protein at lower dose challenge was higher than the protein containing S region alone expressed in yeast system. These results indicate that CHO-derived HBsAg protein containing preS2 and S region can be effectively used for a better immune response as a HBV vaccine.