YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Antibody productivity of HBsAg containing both preS2 and S regions expressed in Chinese hamster ovary cells

Chinese hamster ovary세포에서 발현된 pres2 및 S부위 함유 HBsAg의 항체유발능

  • 정성균 (중앙대학교 약학대학) ;
  • 박정민 (일양약품 중앙연구소 생물공학실) ;
  • 이상봉 (일양약품 중앙연구소 생물공학실) ;
  • 박동우 (일양약품 중앙연구소 생물공학실) ;
  • 김동연 (일양약품 중앙연구소 생물공학실) ;
  • 김기호 (중앙대학교 약학대학) ;
  • 김홍진 (중앙대학교 약학대학)
  • Published : 2001.12.01
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Abstract

Many studies have provided evidences that hepatitis B surface antigen (HBsAg) including preS region could be an ideal candidate for a new hepatitis B virus (HBV) vaccine with higher efficacy. We established CHO cell lines, IY-CHO-2 and IY-CHO-11 expressing high levels of HBsAg containing preS2 and S protein by stable transfection method. These cell lines expressed the correct size (about 1 kb in length) of HBsAg mRNA as expected. The purified protein from the culture supernatants of the clones showed the same sizes as those expressed in native hepatitis B virus (24 kDa, 27 kDa, 34 kDa and 36 kDa). Antibody productivity of CHO-derived HBsAg protein at lower dose challenge was higher than the protein containing S region alone expressed in yeast system. These results indicate that CHO-derived HBsAg protein containing preS2 and S region can be effectively used for a better immune response as a HBV vaccine.

Keywords

References

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