• Journal of Photoscience
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• v.9 no.2
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• pp.451-453
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• 2002

A new concept of "photo" -antisense method has been evaluated, where the inhibition of gene expression by the conventional antisense method is enhanced by photochemical binding between antisense oligonucleotides conjugated with photo-reactive compound and target mRNA or DNA. Fluorescein labeled oligodeoxyribonucleotides (F-DNA) was delivered to cell nuclei in the encapsulated form in multilamellar lecithin liposomes with neutral charge. F-DNA was previously shown to photo-bind to the complementary stranded DNA, and the delivery system using neutral liposome to be effective in normal human keratinocytes. In the present study, we used human kidney cancer G401.2/6TG.1 cell line to be advantageous in reproducible experiments. p53 was adopted as a target gene since antisense sequence information has been accumulated. The nuclear localization ofF-DNA was identified by comparing the fluorescence ofF-DNA with that of Hoechst 33258 under fluorescence microscope. After 7hr incubation to accumulate p53 protein induced by UV -B, p53 protein was quantified by Western blot. After 2hrs from F-DNA application, about 30% of cell population incorporated F-DNA in their nuclei with some morphological change possibly due to liposomal toxicity. Irradiation of visible light longer than 400nm from solar simulator at this time enhanced the inhibitory action of antisense F-DNA. The present results suggest that photo-antisense method is promising to control gene expression in time and space dependent manner. Further improvement of F-DNA delivery to cancer cells in the stability and toxicity is in progress. progress.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.19 no.10
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• pp.1982-1990
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• 1994

We implemented a PLDRO(Phase Locked Dielectric Resonator Oscillator) using the concept of the feedback property of PLL(Phase Locked Loop) for Ku-band(10.95-11.70 GHz). The conventional approaches to a PLDRO design use varactor diode tuning method.. But in theis paper, the PLDRO has the advantage of the frequency sensitivity to changes in the supple voltage of the oscillating device without the frequency-variable part by varactor diode voltage-control. and uses a SPD(Sampling Phase Detector) for phase-comparision. The PLDRO is composed of the DRO phase-locked to the reference signal of UHF band by using a SPD for high frequency stability and can be available for European FSS(Fixed Satellite Service) at 10.00GHz. The PLDRO generates the output power of 8.67 dBm at 10.00 GHz and has a phase noise of -81 dBc/Hz at 10 kHz offset from carrier. The hamonic and spurious characteristics have -42.33 dBc and -65dBc respectively. This PLDRO has much better frequency stability, lower phase noise, and more economical effect for a satellite system than conventional DRO.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.08a
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• pp.253-253
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• 2012

Low temperature SiOx film process has being required for both silicon and oxide (IGZO) based low temperature thin film transistor (TFT) for application of flexible display. In recent decades, from low density and high pressure such as capacitively coupled plasma (CCP) type plasma enhanced chemical vapor deposition (PECVD) to the high density plasma and low pressure such as inductively coupled plasma (ICP) and electron cyclotron resonance (ECR) have been used to researching to obtain high quality silicon oxide (SiOx) thin film at low temperature. However, these plasma deposition devices have limitation of controllability of process condition because process parameters of plasma deposition such as RF power, working pressure and gas ratio influence each other on plasma conditions which non-leanly influence depositing thin film. In compared to these plasma deposition devices, neutral beam assisted chemical vapor deposition (NBaCVD) has advantage of independence of control parameters. The energy of neutral beam (NB) can be controlled independently of other process conditions. In this manner, we obtained NB dependent high crystallized intrinsic and doped silicon thin film at low temperature in our another papers. We examine the properties of the low temperature processed silicon oxide thin films which are fabricated by the NBaCVD. NBaCVD deposition system consists of the internal inductively coupled plasma (ICP) antenna and the reflector. Internal ICP antenna generates high density plasma and reflector generates NB by auger recombination of ions at the surface of metal reflector. During deposition of silicon oxide thin film by using the NBaCVD process with a tungsten reflector, the energetic Neutral Beam (NB) that controlled by the reflector bias believed to help surface reaction. Electrical and structural properties of the silicon oxide are changed by the reflector bias, effectively. We measured the breakdown field and structure property of the Si oxide thin film by analysis of I-V, C-V and FTIR measurement.

• Journal of Advanced Navigation Technology
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• v.9 no.1
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• pp.71-78
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• 2005

Nowadays, the demand of HPI(High-speed Portable Internet) has been gradually increased to support the various services of high speed wire line internet such as xDS. HPI can support high speed internet in anyplace, anytime. For successful development of HPI, the performance should be evaluated according to the cell size and/or the number of users and cell design should be carried out based on these criteria. The previous cellular systems using CDMA technique focus on the establishment of link based on power control but HPI systems consider the QoS (Quality of Service) and its performance based on the scheduling technique. The results from the system level simulation show that the throughput is sensitive to the cell size and the number of users has little impact on it. Moreover, the variation of service delay is more sensitive to the number of users but less to the cell size.

• Korean Journal of Social Welfare
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• v.61 no.3
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• pp.5-28
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• 2009

The purpose of this study is to examine the legal attribute of subsidiarity principle on the National Basic Livelihood Security Act. The principle of subsidiarity is applied to the regulation for 'fundamental principle of benefits' on the National Basic Livelihood Security Act, therefore it functions as legal theory. However, due to its both positive and negative characteristics it becomes to be interpreted and applied in a different meaning depending on the normative characteristics of related laws. In brief, as the passive meaning of subsidiarity principle guarantees the freedom of civilian by limiting national power, it is reasonable in social control norm like criminal law. However, as nation's active role of intervention and support is required in order to protect basic standard of human life according to the principle of Social(welfare)State, the active meaning of subsidiarity principle is reasonable in Social Security law, especially in public assistance. Hence, the principle of subsidiarity on the National Basic Livelihood Security Act becomes to realize, justice, goal fitness and legal stability when it is operated by system with interpreting in an active meaning.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.136.2-136.2
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• 2015

반도체 제조는 chip의 성능 향상 및 단가 하락을 위해 지속적으로 pattern size가 nano size로 감소해 왔고, capacitor 용량은 증가해 왔다. 이러한 현상은 contact hole의 aspect ratio를 지속적으로 증가시킨바, 그에 따라 최적의 HARC (high aspect ratio contact)을 확보하는 적합한 dry etch process가 필수적이다. 그러나 HARC dry etch process는 많은 critical plasma properties 에 의존하는 매우 복잡한 공정이다. 따라서, critical plasma properties를 적절히 조절하여 higher aspect ratio, higher etch selectivity, tighter critical dimension control, lower P2ID과 같은 plasma characteristics을 확보하는 것이 요구된다. 현재 critical plasma properties를 제어하기 위해 다양한 plasma etching 방법이 연구 되어왔다. 이 중 plasma를 낮은 kHz의 frequency에서 on/off 하는 pulsed plasma etching technique은 nanoscale semiconductor material의 etch 특성을 효과적으로 향상 시킬 수 있다. 따라서 본 실험에서는 dual-frequency capacitive coupled plasma (DF-CCP)을 사용하여 plasma operation 동안 duty ratio와 pulse frequency와 같은 pulse parameters를 적용하여 plasma의 특성을 각각 제어함으로써 etch selectivity와 uniformity를 향상 시키고자 하였다. Selective SiO2 contact etching을 위해 top electrode에는 60 MHz pulsed RF source power를, bottom electrode에는 2MHz pulse plasma를 인가하여 synchronously pulsed dual-frequency capacitive coupled plasma (DF-CCP)에서의 plasma 특성과 dual pulsed plasma의 sync. pulsing duty ratio의 영향에 따른 etching 특성 등을 연구 진행하였다. 또한 emissive probe를 통해 전자온도, OES를 통한 radical 분석으로 critical Plasma properties를 분석하였고 SEM을 통한 etch 특성분석과 XPS를 통한 표면분석도 함께 진행하였다. 그 결과 60%의 source duty percentage와 50%의 bias duty percentage에서 가장 향상된 etch 특성을 얻을 수 있었다.

• Journal of Oriental Neuropsychiatry
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• v.20 no.3
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• pp.1-13
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• 2009

Objectives : The glial cell, located in between the blood vessel and nerve cell, takes charge of the cell support, nutrition supply, elimination of body waste, and cell action. GagamSohabhwangwon(GGSH), a chinese traditional medicinal prescription has been used orally for the treatment of seizures, infantile, convulsion, stroke and so forth. This paper examines the effect of the GagamSohabhwangwon(GGSH) essential oil on cell activity and anti oxidation. Methods : MTT assay methods were employed to measure the cell activity based on the amount of the GagamSohabhwangwon(GGSH) essential oil by using primarily cultivated glial cell. In addition, this paper measured a viability of the glial cell after a protein active retarder control to confirm the multiplication of the cell and examined the cell extinction by the active oxygen, an extinction shielding effect with different amount of the GagamSohabhwangwon(GGSH) essential oil to observe anti oxidation. Furthermore, this paper measured a viability of the cell and phosphorylation(phosphorylation) of the protein which affects the multiplication of the glial cell. Results : When controlling the amount of the GagamSohabhwangwon(GGSH), there was a multiplication effect of the primary glial cell, the multiplication of the cell was dependent on the density of the GagamSohabhwangwon. The multiplication power of the primary glial cell was suppressed by PKA inhibiter (H89). In compliance with the active oxygen the extinction of the primary glial cell was dependent on the density of the GagamSohabhwangwon, there was a shielding effect of the cell extinction when GagamSohabhwangwon(GGSH) was preprocessed. When inducing the multiplication of the primary glial cell, phosphorylation of the Akt, BDNF, CREB, ERK and ERM were increased. Conclusions: Based on the results, GagamSohabhwangwon essential oil will have the effect which activates the nervous system cell and protects the cell through anti oxidation.

• Resources Recycling
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• v.10 no.3
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• pp.14-22
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• 2001

For the recycle of coal fly ash generated from power stations, we developed an electrocyclone system which can separate unburned carbon form coal fly ash, based on the fact that coarse fly ash particles contain higher amount of unburned carbon and unburned carbon particles are charged positively, and pure ash particles are charged negatively on contacting each other. Additionally, guide vanes were installed in the cyclone to control the cut size. Two types of electrode, stick and grid type, were designed to investigate the effect of electrode type. Results show that by introducing an electric field inside the cyclone, the yield increases by 5 to 15e1o. But the content of unburned carbon in the clean ash does not change significantly.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.36 no.10
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• pp.971-977
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• 2012

It is well known that the performance of a diesel injector is directly related to the power, emission, and fuel consumption of the diesel combustion engine. In this study, the injection response characteristics of CRDi injectors driven by a solenoid coil and a piezoceramic were investigated by using the AMESim simulation code. Some analytical parameters such as the fuel pressure and hole diameter were considered. From this study, it was shown that the piezo-driven injector had a faster response and had better control capability than the solenoid-driven injector. In addition, it was found that the piezo-driven injector can be utilized more effectively in a multiple injection scheme than a solenoid-driven injector.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38C no.4
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• pp.402-407
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• 2013

In this paper, we consider an interference scenario and interference simulation method of intra-wireless vessel communications using SEAMCAT (Spectrum Engineering Advanced Monte-Carlo Analysis) simulator. The interference between electromagnetic equipment and low power radio apparatus can deteriorate a stability of vessel system and it is necessary to analyze the interference probability between radio devices. The proposed simulation method in the 13.56MHz ISM frequency band shows that the interference effect can be minimized when the distance between the devices is greater than 4.7m and 2.7m in case that the victim receiver (VR) are RFID and remote control(RC) toy, respectively. The proposed interference scenario and simulation method are expected to be helpful in the interference probability analysis and regulation policy in the ISM frequency band.