• 한국동물생명공학회지
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• 제36권2호
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• pp.91-98
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• 2021

The objective of this study was to establish a selection process for high quality sperm in bovine semen using sperm separation by magnetic activation (MACS). For this, semen from 21 Nellore bulls was collected using an artificial vagina. To guarantee the presence of pathologies in the ejaculate, animals previously declassified in four consecutive spermiogram were used. Semen was analyzed in five statuses: (1) fresh semen (fresh); (2) density gradient centrifugation (DGC), percoll column; (3) non-apoptotic fraction after separation by MACS (MAC); (4) apoptotic fraction from the separation (MACPOOR); and (5) MAC followed by DGC (MACDGC). Using a computerized analysis system (CASA), motility was measured. The sperm morphology was evaluated by phase contrast, and the supravital test was completed with eosin/nigrosin staining. For DGC, 20 × 10⁶ cells were used in a gradient of 90% and 45% percoll. MACS used 10 × 10⁶ cells with 20 μL of nanoparticles attached to annexin V, and filtered through the MiniMACS magnetic separation column. Membrane integrity was assessed with SYBR-14/IP and mitochondrial potential with JC-1 by flow cytometry. Processing sperm by MACDGC, was more effective in obtaining samples with high quality sperm, verified by the total of abnormalities in the samples: 35.04 ± 2.29%, 21.50 ± 1.47%, 17.30 ± 1.10%, 30.68 ± 1.94% and 10.50 ± 1.46%, respectively for fresh, DGC, MAC, MACPOOR, and MACDGC. The subpopulation of non-apoptotic sperm had a high number of live cells (82.65%), membrane integrity (56.60%) and mitochondrial potential (83.98%) (p < 0.05). These findings suggest that this nanotechnological method, that uses nanoparticles, is efficient in the production of high-quality semen samples for assisted reproduction procedures in cattle.

• 환경생물
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• 제37권2호
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• pp.204-216
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• 2019

수생태계 생물학적 온전성을 평가하기 위하여 국내 하천 및 하구 923개 지점의 자료를 근거로 한국형 다중형 돌말지수(KMDI)를 개발하고, 이를 금강수계 233개 지점을 대상으로 온전성평가를 실시하여 단일형 돌말지수들과 비교 검토하였다. KMDI의 개발은 1) 먼저 선행문헌들을 참고로 300개 이상의 메트릭을 추출하고, 2) 이 중 설명력이 높은 46개 후보 메트릭을 선택하며, 3) 각 메트릭 값들의 변이성, 중복성, 변별력, 환경에 대한 민감성 등을 검증하고, 최종적으로 5가지의 메트릭을 선정하였다. KMDI는 매트릭의 단순합으로 표현하고, 환경요인들에 대한 신뢰성 검토 결과, 토지이용, BOD, TN, 전기전도도 등에 대해 높은 민감성 및 설명력을 보였으나 단일형 돌말지수들 보다는 동일 지점의 생물학적 온전성은 다소 높게 평가되는 특성을 보였다. 추후 국내는 물론 지역에 상관없이 적용가능한 설명력 높은 매트릭의 발굴 및 정확한 돌말류 분석 연구가 뒷따라야 될 것으로 판단되었다.

• 한국정보과학회논문지:소프트웨어및응용
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• 제30권5_6호
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• pp.587-597
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• 2003

일반적으로 C언어는 고신뢰도를 요구하는 소프트웨어에 적합하지 않다고 알려졌음에도 불구하고 적지 않은 수의 안전성이 중요시되는(safety-critical) 시스템들이 C언어로 구현되었거나 C언어를 기반으로 개발되고 있다. 본 연구의 목적은 C언어의 안전한 subset을 정의하고 이 subset 언어로 작성된 프로그램을 SPARK Ada로 변환하여 SPARK의 분석 도구들을 사용해 프로그램의 안전성을 분석하는 데 있다. SPARK은 Ada의 안전한 subset으로 고신뢰도를 요하는 시스템을 구현하는데 성공적으로 사용되어왔다. SPARK으로 변환된 C 프로그램은 SPARK 수준의 안전성을 갖게 되며 SPARK의 분석 도구인 Examiner를 통해 프로그램의 정확성 검증 등의 분석을 할 수 있다. 본 연구에서는 엘리베이터 컨트롤러 사례 연구를 통해 정의한 subset이 현실적인 시스템을 구현하기에 부족하지 않음을 발견하였으며, SPARK Ada로의 변환을 자동화해주는 변환기를 구현하였다.

• Coupled systems mechanics
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• 제7권3호
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• pp.297-319
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• 2018

In this paper, the vibro-acoustic behaviors of vibrational cylindrical shells are investigated by using structural intensity approach. The reducing interior noise method for vibrating cylindrical shells is proposed by altering and redistributing the structural intensity through changing the damping property of the structure. The concept of proposed novel method is based on the properties of structural intensity distribution on cylindrical shells under different load and damping conditions, which can reflects power flow in the structures. In the study, the modal formulas of structural intensity are developed for the steady state vibration of cylindrical shell structures. The detailed formulas of structural intensity are derived by substituting modal quantities, in which the effect of main parameters such as weight coefficients and distribution functions on structure intensity are analyzed and discussed. Numerical simulations are first carried out based on the structural intensity analytical solutions of modal formulas. Through simulating the coupling vibration and acoustical radiation problems of cylindrical shell, the relationship between vibro-acoustic and structural intensity distribution is derived. We find that for cylindrical shell, by properly arranging damping conditions, the structural intensity can be efficiently changed and further the noise property can be improved. The proposed methodology has important implications and potential applications in the vibration and noise control of fuselage structure.

• Journal of Ginseng Research
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• 제42권3호
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• pp.379-388
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• 2018

Background: Ginsenosides are the main ingredients of Korean Red Ginseng. They have extensively been studied for their beneficial value in neurodegenerative diseases such as Parkinson's disease (PD). However, the multitarget effects of Korean Red Ginseng extract (KRGE) with various components are unclear. Methods: We investigated the multitarget activities of KRGE on neurological dysfunction and neurotoxicity in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of PD. KRGE (37.5 mg/ kg/day, 75 mg/kg/day, or 150 mg/kg/day, per os (p.o.)) was given daily before or after MPTP intoxication. Results: Pretreatment with 150 mg/kg/day KRGE produced the greatest positive effect on motor dysfunction as assessed using rotarod, pole, and nesting tests, and on the survival rate. KRGE displayed a wide therapeutic time window. These effects were related to reductions in the loss of tyrosine hydroxylase-immunoreactive dopaminergic neurons, apoptosis, microglial activation, and activation of inflammatory factors in the substantia nigra pars compacta and/or striatum after MPTP intoxication. In addition, pretreatment with KRGE activated the nuclear factor erythroid 2-related factor 2 pathways and inhibited phosphorylation of the mitogen-activated protein kinases and nuclear factor-kappa B signaling pathways, as well as blocked the alteration of blood-brain barrier integrity. Conclusion: These results suggest that KRGE may effectively reduce MPTP-induced neurotoxicity with a wide therapeutic time window through multitarget effects including antiapoptosis, antiinflammation, antioxidant, and maintenance of blood-brain barrier integrity. KRGE has potential as a multitarget drug or functional food for safe preventive and therapeutic strategies for PD.

• 한국지반공학회논문집
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• 제24권8호
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• pp.13-23
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• 2008

다양한 토목 건축 구조물의 하부구조로 많이 사용되는 콘크리트 말뚝기초는 상부 토목 건축 구조물의 안전에 있어 매우 중요한 요소이다. 본 논문에서는 이러한 말뚝의 비파괴적인 건전성 평가를 위하여, 범용적인 파의 위상 그룹속도 결정법인 HWAW(Harmonic Wavelet Analysis of Wave)방법을 말뚝의 비파괴 시험에 적용하였다. 제안된 방법은 말뚝자체의 건전성 평가와 선단지지말뚝의 거동에 매우 중요한 말뚝 선단부 상태(경계조건)를 자동적으로 결정할 수 있다. 제안된 방법을 검증하기 위하여 ABAQUS를 사용한 수치모의 실험을 수행하였으며, 실제 모형말뚝을 제작하여 공기중 실험과 실제 지반조건을 모사하기 위한 토조내 실험을 수행하였다. 이러한 수치모의 실험과 실제 모의 실험을 통하여 말뚝의 비파괴 건전성 평가에 있어서의 제안된 방법의 타당성을 확인하였다.

• Journal of Astronomy and Space Sciences
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• 제24권4호
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• pp.451-456
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• 2007

장치로부터 발생된 데이터는 소프트웨어에서 사용되기 위해 최대한 정확한 시간에 데이터의 완결성을 가지고 해당 태스크에 전달되어야 한다. 이 과정에서 구조적인 위험성과 성능 저하가 발생할 원인이 잠재되어 있다. 이 논문은 장치와 태스크 사이에서 I/O 데이터 교환이 발생할 시 충돌에 의해서 발생가능한 데이터 손실을 예방할 수 있는 방안을 기술한다. 이 논문에서 기술하는 방안에 대한 메커니즘의 예시도를 제안하고 그 효과 및 장단점을 고찰하였다.

• 시스템엔지니어링학술지
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• 제12권2호
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• pp.101-114
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• 2016

A model-based systems engineering (MBSE) approach to implementing hardware-based network cybersecurity controls for APR1400 non-safety data network is presented in this work. The proposed design was developed by implementing packet filtering and deep packet inspection functions to control the unauthorized traffic and malicious contents. Denial-of-Service (DoS) attack was considered as a potential cybersecurity issue that may threaten the data availability and integrity of DCS gateway servers. Logical design architecture was developed to simulate the behavior of functions flow. HDL-based physical architecture was modelled and simulated using Xilinx ISE software to verify the design functionality. For effective modelling process, enhanced function flow block diagrams (EFFBDs) and schematic design based on FPGA technology were together developed and simulated to verify the performance and functional requirements of network security controls. Both logical and physical design architectures verified that hardware-based cybersecurity controls are capable to maintain the data availability and integrity. Further works focus on implementing the schematic design to an FPGA platform to accomplish the design verification and validation processes.

• The Journal of Asian Finance, Economics and Business
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• 제7권7호
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• pp.453-462
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• 2020

The research postulates the conceptualization of talent in the Vietnamese banking sector via examining the factors pertaining to the concept of talent and talent management (TM) in the sector. This study applied qualitative research methods. A total of 20 managers and directors of ten banks (three public, four private and three foreign banks) were recruited for semi-structured interviews. The findings revealed that a combination of interconnected soft skills, learning ability, flexibility, technology adaptability, integrity and risk management skills contributes to talent identification. Managers in some private banks construed talent to be commensurate with high performance and high potential, whereas managers in public banks and foreign banks mainly relied on performance results in talent recognition. Moreover, talented employees holding sales-related jobs are given the most attention by management in the studied banks. Regarding practical implications, the banking community and practitioners' focus should be imparted to soft skills development and integrity control in order to foster employee performance and attitudes. Attention should be paid not only to sales positions, but also to other positions within the bank. This study is one of a few which explores talent concepts and TM approaches in the banking sector in general and Vietnamese banking field in particular.

• Biomolecules & Therapeutics
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• 제3권3호
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• pp.242-244
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• 1995

In vitro cell culture system has been proposed as a promising alternative model to in vivo skin irritation test. These studies were performed to screen the cytotoxicity effects of surfactants using normal human skin fibroblasts. Cell membrane integrity assessed by the leakage of lactate dehydrogenase (LDH) and mitochondrial integrity by MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromides reduction test were affected in a dose dependent manner. The irritation potential of surfactants to human skin patch test, and the changes of capillary permeability by rabbit intradermal safety test were assessed as in vivo methods. Our results suggest that LDH leakage assay and MTT reduction test using cultured human fibroblasts could be predictive for the irritancy of various surfactants in human, and LDH assay is superior correlated with in vivo test (r=0.886) to MTT test with in vivotest (r=0.757).