• Journal of Microbiology and Biotechnology
• /
• v.33 no.3
• /
• pp.288-298
• /
• 2023

Host defense peptides are expressed in various immune cells, including phagocytic cells and epithelial cells. These peptides selectively alter innate immune pathways in response to infections by pathogens, such as bacteria, fungi, and viruses, and modify the subsequent adaptive immune environment. Consequently, they play a wide range of roles in both innate and adaptive immune responses. These peptides are of increasing importance due to their broad-spectrum antimicrobial activity and their functions as mediators linking innate and adaptive immune responses. This review focuses on the pleiotropic biological functions and related mechanisms of action of human host defense peptides and discusses their potential clinical applications.

• BMB Reports
• /
• v.56 no.6
• /
• pp.335-340
• /
• 2023

During normal physiological and abnormal pathophysiological conditions, all cells release membrane vesicles, termed extracellular vesicles (EVs). Growing evidence has revealed that EVs act as important messengers in intercellular communication. EVs play emerging roles in cellular responses and the modulation of immune responses during virus infection. EVs contribute to triggering antiviral responses to restrict virus infection and replication. Conversely, the role of EVs in the facilitation of virus spread and pathogenesis has been widely documented. Depending on the cell of origin, EVs carry effector functions from one cell to the other by horizontal transfer of their bioactive cargoes, including DNA, RNA, proteins, lipids, and metabolites. The diverse constituents of EVs can reflect the altered states of cells or tissues during virus infection, thereby offering a diagnostic readout. The exchanges of cellular and/or viral components by EVs can inform the therapeutic potential of EVs for infectious diseases. This review discusses recent advances of EVs to explore the complex roles of EVs during virus infection and their therapeutic potential, focusing on HIV-1.

• Journal of Integrative Natural Science
• /
• v.3 no.1
• /
• pp.49-53
• /
• 2010

Molecular docking is a critical event which mostly forms Van der waals complex in molecular recognition. Since the majority of developed drugs are small molecules, docking them into proteins has been a prime concern in drug discovery community. Since the binding pose space is too vast to cover completely, many search algorithms such as genetic algorithm, Monte Carlo, simulated annealing, distance geometry have been developed. Proper evaluation of the quality of binding is an essential problem. Scoring functions derived from force fields handle the ligand binding prediction with the use of potential energies and sometimes in combination with solvation and entropy contributions. Knowledge-based scoring functions are based on atom pair potentials derived from structural databases. Forces and potentials are collected from known protein-ligand complexes to get a score for their binding affinities (e.g. PME). Empirical scoring functions are derived from training sets of protein-ligand complexes with determined affinity data. Because non of any single scoring function performs generally better than others, some other approaches have been tried. Although numerous scoring functions have been developed to locate the correct binding poses, it still remains a major hurdle to derive an accurate scoring function for general targets. Recently, consensus scoring functions and target specific scoring functions have been studied to overcome the current limitations.

• Wind and Structures
• /
• v.16 no.6
• /
• pp.561-577
• /
• 2013

Rational Functions are used to express the self-excited aerodynamic forces acting on a flexible structure for use in time-domain flutter analysis. The Rational Function Approximation (RFA) approach involves obtaining of these Rational Functions from the frequency-dependent flutter derivatives by using an approximation. In the past, an algorithm was developed to directly extract these Rational Functions from wind tunnel section model tests in free vibration. In this paper, an algorithm is presented for direct extraction of these Rational Functions from section model tests in forced vibration. The motivation for using forced-vibration method came from the potential use of these Rational Functions to predict aerodynamic loads and response of flexible structures at high wind speeds and in turbulent wind environment. Numerical tests were performed to verify the robustness and performance of the algorithm under different noise levels that are expected in wind tunnel data. Wind tunnel tests in one degree-of-freedom (vertical/torsional) forced vibration were performed on a streamlined bridge deck section model whose Rational Functions were compared with those obtained by free vibration for the same model.

• Journal of Forest and Environmental Science
• /
• v.29 no.1
• /
• pp.99-108
• /
• 2013

This study was conducted to evaluate the non-timber forest functions of the Research Forest area of Kangwon National University. In this study, forest recreation function was investigated through survey method answered by forest management experts. The evaluation factors for forest recreation function were separated into external and internal factors. Each factor was classified into intermediate element and detailed element. Therefore in this study, the evaluation factors of forest recreation function were divided into two groups which are the potential of external factors and the suitability of internal factors. Potential of the external factors were divided into accessibility, location, landscape and induction factors while the suitability of internal factor was divided into stand structure, forest structure and human impact. The priority of the potential of external factors was in order that location, accessibility, induction factors and landscape. The most important factors for the location, accessibility, induction and landscape factors are based on regional characteristics.

• Biomolecules & Therapeutics
• /
• v.25 no.5
• /
• pp.471-481
• /
• 2017

The canonical transient receptor potential channels (TRPCs) constitute a series of nonselective cation channels with variable degrees of $Ca^{2+}$ selectivity. TRPCs consist of seven mammalian members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, and TRPC7, which are further divided into four subtypes, TRPC1, TRPC2, TRPC4/5, and TRPC3/6/7. These channels take charge of various essential cell functions such as contraction, relaxation, proliferation, and dysfunction. This review, organized into seven main sections, will provide an overview of current knowledge about the underlying pathogenesis of TRPCs in cardio/cerebro-vascular diseases, including hypertension, pulmonary arterial hypertension, cardiac hypertrophy, atherosclerosis, arrhythmia, and cerebrovascular ischemia reperfusion injury. Collectively, TRPCs could become a group of drug targets with important physiological functions for the therapy of human cardio/cerebro-vascular diseases.

• Food Science of Animal Resources
• /
• v.31 no.5
• /
• pp.631-640
• /
• 2011

Milk oligosaccharides are the complex mixture of six monosaccharides namely, D-glucose, D-galactose, N-acetyl-glucosamine, N-acetyl-galactosamine, L-fucose, and N-acetyl-neuraminic acid. The mixture is categorized as neutral and acidic classes. Previously, 25 oligosaccharides in bovine milk and 115 oligosaccharides in human milk have been characterized. Because human intestine lacks the enzyme to hydrolyze the oligosaccharide structures, these substances can reach the colon without degradation and are known to have many health beneficial functions. It has been shown that this fraction of carbohydrate can increase the bifidobacterial population in the intestine and colon, resulting in a significant reduction of pathogenic bacteria. The role of milk oligosaccharides as a barrier against pathogens binding to the cell surface has recently been demonstrated. Milk oligosaccharides have the potential to produce immuno-modulation effects. It is also well known that oligosaccharides in milk have a significant influence on intestinal mineral absorption and in the formation of the brain and central nervous system. Due to its structural resemblance, bovine milk is considered to be the most potential source of oligosaccharides to produce the same effect of oligosaccharides present in human milk. This review describes the characteristics and potential health benefits of milk oligosaccharides as well as the prospects of oligosaccharides in bovine milk for use in functional foods.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.1
• /
• pp.127-136
• /
• 2015

Cupredoxin-like proteins are mainly copper-binding proteins that conserve a typical rigid Greek-key arrangement consisting of an eight-stranded β-sandwich, even though they share as little as 10-15% sequence similarity. The electron transport function of the Cupredoxins is critical for respiration and photosynthesis, and the proteins have therapeutic potential. Despite their crucial biological functions, the identification of the distant Cupredoxin homologs has been a difficult task due to their low sequence identity. In this study, the overlapped conserved residue (OCR) fingerprint for the Cupredoxin superfamily, which consists of conserved residues in three aspects (i.e., the sequence, structure, and intramolecular interaction), was used to detect the novel Cupredoxin homologs in the NCBI non-redundant protein sequence database. The OCR fingerprint could identify 54 potential Cupredoxin sequences, which were validated by scanning them against the conserved Cupredoxin motif near the Cu-binding site. This study also attempted to model the 3D structures and to predict the functions of the identified potential Cupredoxins. This study suggests that the OCR-based approach can be used efficiently to detect novel homologous proteins with low sequence identity, such as Cupredoxins.

• Journal of Internet Computing and Services
• /
• v.18 no.6
• /
• pp.1-6
• /
• 2017

For the algorithm of cross-information potential with delta functions (CIPD) which has superior performance in impulsive noise environments, a new method of employing the information of power variations of magnitude controlled input (MCI) in the weight update equation of the CIPD is proposed in this paper where the input of CIPD is modified by the Gaussian kernel of error. To prove its effectiveness compared to the conventionalCIPD algorithm, the distance between the current weight vector and its previous one is analyzed and compared under impulsive noise. In the simulation results the proposed method shows a two-fold improvement in steady state stability, faster convergence speed by 1.8 times, and 2 dB - lower minimum MSE in the impulsive noise situation.

• Korean Journal of Biological Psychiatry
• /
• v.22 no.2
• /
• pp.29-33
• /
• 2015

The brain maintains homeostasis and normal microenvironment through dynamic interactions of neurons and neuroglial cells to perform the proper information processing and normal cognitive functions. Recent post-mortem investigations and animal model studies demonstrated that the various brain areas such as cerebral cortex, hippocampus and amygdala have abnormalities in neuroglial numbers and functions in subjects with mental illnesses including schizophrenia, dementia and mood disorders like major depression and bipolar disorder. These findings highlight the putative role and involvement of neuroglial cells in mental disorders. Herein I discuss the physiological roles of neuroglial cells such as astrocytes, oligodendrocytes, and microglia in maintaining normal brain functions and their abnormalities in relation to mental disorders. Finally, all these findings could serve as a useful starting point for potential therapeutic concept and drug development to cure unnatural behaviors and abnormal cognitive functions observed in mental disorders.