• Proceedings of the Korean Environmental Health Society Conference
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• 2005.06a
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• pp.319-322
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• 2005

Porous building materials are not only sources of indoor air pollutants such as volatile organic compounds (VOCs) but they are also strong sinks of these pollutants. Volatile organic compounds have been implicated in impaired spermatogenesis, increase in the incidence of malformed sperm and decrease in the percentage of moving sperm. The aim of this study was to determine and compare the direct effects of various volatile organic compounds (phenol, formaldehyde; HCHO, ethanol, toluene, styrene) on motility and survival rate of human sperm in vitro. Semen samples from 3 health subjects were prepared using swim-up method and 1-10mM volatile organic compounds were added to the test medium. HCHO and phenol produced significant decreases in the motility and survival rate with a different potency. The most potent inhibition of motility and survival rate was observed after exposure to HCHO. Less than 1mM HCHO significantly inhibited sperm motility. When ethanol is added directly to sperm, at concentrations equivalent to that in serum after heavy drinking, these damaging effects were lowest compared with other volatile organic compounds. Present study shows that each compound has differential toxic potency to human sperm and we need special caution for the use of HCHO and phenol.

• Journal of Korean Medical classics
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• v.30 no.2
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• pp.45-57
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• 2017

Objectives : There exists a number of books that deal with curing diseases with foods. This includes Shennongbencaojing from Han Dynasty era, Zanyin's Shiloaoxingan and Mengxi's Shilaobencao from Tang Dynasty era, Husihui's Yinshanzhengyao from Won Dynasty era, and Feiboxiong's Sikgamboncho from Qing Dynasty era. This paper aims to delve into the food treatment revealed in Sikgamboncho, which is the latest book in the preceeding list, and studied its differences when compared to other medical books that deal with food treatment methods. Methods : The format and contents of Feiboxiong's Sikgamboncho were studied. For its format, Shilaoaoxingan, Yinshanzhengyao, Shikryochanyo, and Donguibogam were used to compare their formats with that of Sikgamboncho in order to discern the unique characteristics found in the book. For its contents, the paper studied food ingredients and food treatment separately, and then studied the historical significance of Feiboxiong's Sikgamboncho. Results : Sikgamboncho categorizes 12 types of grain, 22 types of vegetables, 7 types of cucumis calyx, 27 types of fruit, 9 types of flavor, 4 types of bird, 4 types of animal, 10 types of fish with scales, 4 types of carapace, and 2 types of worm. The book explains each ingredient's harvest period, its nature, potency, and taboos. The food treatment section explains 74 types of treatments that are divided between the wind, cold, summer heat, dampness, dryness category and the qi, blood phlegm, deficiency, and excess category. Each treatment contains its potency and recipe. Conclusions : Feiboxiong underlined the importance of ingredient through placing significance even to the order of ingredients. Out of the total 74 food treatments, 25 treatments were dedicated to deal with weakness. Feiboxiong attempted to prevent human body from becoming weak, which reflects his emphasis on the importance of food and wellness.

• BMB Reports
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• v.48 no.2
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• pp.91-96
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• 2015

Cells express several antioxidant enzymes to scavenge reactive oxygen species (ROS) responsible for oxidative damages and various human diseases. Therefore, antioxidant enzymes are considered biomedicine candidates. Among them, extracellular superoxide dismutase (SOD3) had showed prominent efficacy against asthma and inflammation. Despite its advantages as a biomedicine, the difficulty in obtaining large quantity of active recombinant human SOD3 (rhSOD3) has limited its clinical applications. We found that a significant fraction of over-expressed rhSOD3 was composed of the inactive apo-enzyme and its potency against inflammation depended on the rate of metal incorporation. Also, purified rhSOD3 was unstable and lost its activity very quickly. Here, we suggest an ideal preparative method to express, purify, and store highly active rhSOD3. The enzymatic activity of rhSOD3 was maximized by incorporating metal ions into rhSOD3 after purification. Also, albumin or polyethylene glycol prevented rapid inactivation or degradation of rhSOD3 during preparative procedures and long-term storage.

• Biomolecules & Therapeutics
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• v.10 no.2
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• pp.124-128
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• 2002

Hantaan (HTN) and Puumala (PUU) viruses are major etiological agents of hemorrhagic fever with renal syndrome (HFRS), an important public health problem in Korea after the Korean War. The objective of present study was to determine allergenic potency of an inactivated combination vaccine against HTN and PUU viruses inflection. As a series of allergenicity assessment, a homologous active systemic anaphylaxis (ASA) and homologous/heterologous passive cutaneous anaphylaxis (PCA) tests using the mice and guinea pigs were carried out. In the ASA test, no anaphylactic symptoms were observed in the guinea pigs sensitized with the vaccine alone as well as the vaccine emulsified with an adjuvant. By homologous PCA test, the vatscine did not induced the potential IgE antibody production in the sera obtained from the sensitized guinea pigs. In addition, IgE against the vaccine was not significantly enhanced from the mice inoculated with the vaccine, which was judged by the heterologous PCA test in rats. On the other hand, the inoculation of ovalbumin appeared to allergenic reactions both in the ASA and PCA tests. The results suggest that a combination vaccine against HW and PUU viruses have no allergenic potential in mice or guinea pigs.

• Journal of the Korean Society of Clothing and Textiles
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• v.30 no.7 s.155
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• pp.1012-1024
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• 2006

The purpose of this study is to investigate the effect of color, shirt tone, tie tone, perceiver's gender on impression formation by applying tone on tone coloration of shirt and necktie: coloration of two colors. The experimental materials developed for this study were a set of stimulus and response scales. The stimuli were 32 upper body photographs which were color outputs by CAD system(4D-box program). We unified those colors of shirts and neckties same, then made shirt and necktie tone differently. 27 bi-polar adjectives, each of which was graded into seven in its degree, were used to evaluate the impression. The subjects of this research were 192 male and 192 female college students in Gyeongnam province including Jinju City. The data was analyzed by using SPSS program. Analysis methods were four-way ANOVA. The items of the adjectives were classified into 5 impression dimension. - potency, activity, attractiveness, visibility and tenderness. Color, shirt tone, tie tone, and perceiver gender influenced on 5 impression formation by interaction as well as independently. Blue tie with vivid, light or dull tone had an effect on the formation of potency impression, vivid or light tone of shirt affected activity regardless of color or tie tone. As shirt and tie had different tone, effect on the formation of attractiveness impression, light tone had a negative effect on visibility but positive effect on tenderness. red color were more effective than blue expressed on visibility and tenderness in shirt or tie.

• Journal of Food Hygiene and Safety
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• v.13 no.3
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• pp.189-195
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• 1998

Comparative insecticidal efficiency between compounded pyrethroids and its preparations cooperated with several synergists was studied. As main components, Group A contained Permethrine.Phthalthrine, Group B contained Permethrine.Furameth rine and Group C contained Phthalthrine.Dichlorvos. As synergists Piperonyl Butoxide (P.B.O.), N-Octylbicycloheptene dicarboximide (MGK-264), Octachlorodipropylether (8-421) was cooperated in each group. These preparations was treated to either Blattella G. or Musca D. for comparing about insecticidal efficiency[Knock-down rate ($KT_{50},\;KT_{90}$) and cumulative mortaility(percent/hrs)]. When the synergists was added to each Group, the insecticidal efficiency was improved. The most potent synergists was P.B.O. and the potency was decreased in the rank order of S-421>MGK-264. The more the concentration of synergists was increased, the more the insecticidal efficiency was improved. The rank order was $1:5{\geq}1:4>1.3$ in all three groups. The mortality (percent) according to time was similar to either $KT_{50}\;or\;KT_{90}$. Knock-down rate was appropriate parameter for the indicator about potency.

• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.117-126
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• 2010

A chitinase (CHT) and a protease (PRO) were purified from the culture supernatant of Serratia sp. TKU017, with shrimp shell as the sole carbon/nitrogen source. The molecular masses of CHT and PRO determined by SDS-PAGE were approximately 65 kDa and 53 kDa, respectively. CHT was inhibited by $Mn^{2+}$ and $Cu^{2+}$, and PRO was inhibited by most tested divalent metals and EDTA. The optimum pH, optimum temperature, pH stability, and thermal stability of CHT and PRO were pH 5, $50^{\circ}C$, pH 5-7, and <$50^{\circ}C$, and pH 9, $40^{\circ}C$, pH 5-11, and <$40^{\circ}C$, respectively. PRO retained 95% of its protease activity in the presence of 0.5 mM SDS. The result demonstrates that PRO is an SDS-resistant protease and probably has a rigid structure. The $4^{th}$-day supernatant showed the strongest antioxidant activity (70%, DPPH scavenging ability) and the highest total phenolic content ($196{\pm}6.2\;{\mu}g$ of gallic acid equiv./ml). Significant associations between the antioxidant potency and the total phenolic content, as well as between the antioxidant potency and free amino groups, were found for the supernatant. With this method, we have shown that shrimp shell wastes can be utilized and it is effective in the production of enzymes and antioxidants, facilitating its potential use in industrial applications and functional foods.

• Environmental Analysis Health and Toxicology
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• v.30
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• pp.14.1-14.7
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• 2015

Objectives Cigarette smoking is associated with carcinogenesis owing to the mutagenic and genotoxic effects of cigarette smoke. The aim of this study was to evaluate the mutagenic and genotoxic effects of Korean cigarettes using in vitro assays. Methods We selected 2 types of cigarettes (TL and TW) as benchmark Korean cigarettes for this study, because they represent the greatest level of nicotine and tar contents among Korean cigarettes. Mutagenic potency was expressed as the number of revertants per ${\mu}g$ of cigarette smoke condensate (CSC) total particulate matter whereas genotoxic potency was expressed as a concentration-dependent induction factor. The CSC was prepared by the International Organization for Standardization 3308 smoking method. CHO-K1 cells were used in vitro micronucleus (MNvit) and comet assays. Two strains of Salmonella typhimurium (Salmonella enterica subsp. enterica ; TA98 and TA1537) were employed in Ames tests. Results All CSCs showed mutagenicity in the TA98 and TA1537 strains. In addition, DNA damage and micronuclei formation were observed in the comet and MNvit assays owing to CSC exposure. The CSC from the 3R4F Kentucky reference (3R4F) cigarette produced the most severe mutagenic and genotoxic potencies, followed by the CSC from the TL cigarette, whereas the CSC from the TW cigarette produced the least severe mutagenic and genotoxic potencies. Conclusions The results of this study suggest that the mutagenic and genotoxic potencies of the TL and TW cigarettes were weaker than those of the 3R4F cigarette. Further study on standardized concepts of toxic equivalents for cigarettes needs to be conducted for more extensive use of in vitro tests.

• Journal of Microbiology and Biotechnology
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• v.18 no.2
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• pp.355-364
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• 2008

Mushrooms are regarded as one of the well-known foods and biopharmaceutical materials with a great deal of interest. ${\beta}$-Glucan is the major component of mushrooms that displays various biological activities such as antidiabetic, anticancer, and antihyperlipidemic effects. In this study, we explored the molecular mechanism of its immunostimulatory potency in immune responses of macrophages, using exopolysaccharides prepared from liquid culture of Lentinus edodes. We found that fraction II (F-II), with large molecular weight protein polysaccharides, is able to strongly upregulate the phenotypic functions of macrophages such as phagocytic uptake, ROS/NO production, cytokine expression, and morphological changes. F-II triggered the nuclear translocation of NF-${\kappa}B$ and activated its upstream signaling cascades such as PI3K/Akt and MAPK pathways, as assessed by their phosphorylation levels. The function-blocking antibodies to dectin-1 and TLR-2, but not CR3, markedly suppressed F-II-mediated NO production. Therefore, our data suggest that mushroom-derived ${\beta}$-glucan may exert its immunostimulating potency via activation of multiple signaling pathways.

• The Korean Journal of Pharmacology
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• v.1 no.1 s.1
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• pp.37-46
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• 1965

1. In the tortoise, Amyda japonica, a cold-blooded animal readily available in this country, the role of catecholamines in the regulation of free fatty acids(FFA) metabolism was investigated in both in vivo and in vitro studies. 2. Norepinephrine elevated both FFA and glucose levels in plasma. 3. When $50{\mu}g/kg$ of Epinephrine, Norepinephrine and Isopropylarterenol were administered intravenously, the relative effectiveness of mobilizing FFA was in the descending order of potency-Epinephrine, Norepinephrine and Isopropylarterenol. 4. In order to exclude the 'tonic influence of the endogenous catecholamines', reserpine was given to some animals. Two days after the reserpine-treatment, glucose showed a significant increase over the solely vehicle treated controls, FFA but an insignificant one. Excised auricles from those animals showed a diminished response to tyramine. Seven days after the treatment, however, when the depletion of catecholamines from the tissue stores seemed to be complete, judged from the absence of the response of isolated auricles to tyramine, both FFA and glucose levels were definitely lowered. 5. In in vitro experiments Epinephrine enhanced the FFA-release from the adipose tissue. The effect increased proportionately with the concentration until a maximal effect was attained at a concentration of 1x $10^5$ g/ml. 6. The order of potency in releasing FFA from adipose tissue in vitro was the same as in vitro, i.e., Epinephrine, Norepinephrine and Isopropylarterenol, but the differences were much less marked. 7. Ergotamine exerted no lipolytic action, but inhibited the lipolytic effect ef Epinephrine significantly. 8. Nethalide showed a slight lipolytic effect per se but inhibited the Epinephrine-induced lipolysis significantly. 9. Catecholamines play an important role in regulating FFA metabolism in the cold-blooded animal, just as in the warm-blooded animals, and the tortoise, Amyda japonica, may be used in the studies of fat metabolism as well as the rat.