• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2002.04a
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• pp.93-96
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• 2002

감자는 오랜 재배 역사를 가진 세계4대 식량작물로 영양가가 풍부하며 생산량이 어느 작물보다 많기 때문에 전략식량으로 많이 이용되고 있다. 최근 가공산업의 발달로 감자의 수요가 급격히 증가함에 따라 감자의 대량 생산에 관련된 많은 연구들이 수행되어 왔으며, 그 중 미세증식 기법은 단기간에 대량 생산할 수 있다는 점에서 각광을 받고 있다. (중략)

• Journal of Plant Biology
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• v.37 no.1
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• pp.17-25
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• 1994

Two potato (Solanum tuberosum L.) cultivars were transformed by Agrobacterium tumefaciens harboring cauliflower mosaic virus (CaMV) 35S promoter and $\beta$-glucuronidase (GUS) gene. Expression patterns of the CaMV 35S promoter according to tissue types and developmental stages, and genetic stability of GUS gene were investigated in the clonal progenies of transgenic potatoes. Kanamycin-resistant shoot emerged from tuber disc after 4 weeks of culture, and root was induced 6 weeks after culture on the selection medium. Shooting frequency of cvs. Superior and Dejima were 43% and 27%, respectively. Mature transformants and their clonal progenies showed no phenotypical abnormality. GUS activity was expressed primarily at parenchymatous cells of phloem tissue around the vascular cambium in the stem and root, and higher activity was found at the apical meristem of shoot, root and adventious shoot bud. GUS activity was higher at tubers of young explants than at stored tubers. These facts indicate that expression level of the CaMV 35S promoter differed according to tissue types and developmental stages of the organs. The GUS gene was stably inherited to each clonal progeny and normally expressed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.201-206
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• 1999

For the mass production of plug seedlings in cultivar ‘Dejima’ potato (Solanum tuberosum L.) the optimal apical cutting diameter for rooting and rapid multiplication of stem cuttings in hydroponics were determined. In addition, the best planting date was predicted to increase tuber yield of plug seedlings at fall cropping in Cheju-Do, Korea. Days to initial rooting decreased as the cutting diameter was reduced. Plant height, leaf number, root length and root weight per plant were favorable as the cutting diameter was small. The ideal cutting diameter was 1-2 mm in this experiment. In the hydroponic cultures, the Japanese standard (JS) nutrient solution was the most effective for multiplication of stem cuttings. It was able to propagate more than 20 times a month from a single mother plant. Viability of plants, which were derived from plug seedlings using stem cuttings, was excellent when transplanted to the field. The number of tubers and tuber yield in both of the plug seedlings and seed potato planting plots were high when planted on 25 August. The number and yield were reduced when planted on 15 August, 5 September and 15 September. The degree of decrease of tuber yield in the plug seedling planting plot however, was lower than that of seed potatoes when the planting date was late. In the case of small tubers (under 30 g), the number of tubers and tuber yield were evidently increased in the seed potato tuber planting plot; the yield of large tuber (over 80g) in the plug seedling planting plot was higher than that of the seed potato. The total tuber yield per plant in the plug seedling planting plot was less than that of the seed potato; therefore, in order to increase tuber yield it was necessary to increase field plant density.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.24-31
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• 2013

This study was conducted to determine the characteristics of quality and yield in potatoes grown at paddy field before rice transplantation during the spring season. Three potato cultivars ('Jowon', 'Haryeong', and 'Goun') were grown in Gangneung (asl 5 m) and Seocheon (asl 20 m). In both locations, weather condition belonged to the fourth zone (spring cropping) in potato production location's distribution of Korea. Daily mean soil temperature in both the locations was $0.2-0.6^{\circ}C$ lower than air temperature, while soil moisture was adequate level to potato growth in spite of spring drought. TR ratio was not affected by location, but by cultivar. Specific gravity, starch content, dry matter rate, and yield were significantly influenced by location and by cultivar. There was no difference in total tuber number by location, however there was a large gap in marketable tuber yield according to locations and cultivars. There were high negative relationships between yield and main qualities such as dry matter rate and starch content, while high positive correlation was observed between main qualities. It was possible to produce potato before rice transplanting at drained paddy fields located in representative two locations of potato spring cropping and their characteristics in growth and quality were similar to those generally well known in upland cultivation. Paddy field was thought to be more favorable than upland in terms of available soil moisture supply against spring drought. Further research, however, was needed to increase soil temperature and also preliminary review on proper cultivar according to location seemed to be needed for high yield.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.6
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• pp.463-468
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• 2013

The level of nitrous oxide ($N_2O$), a long-lived greenhouse gas, in atmosphere has increased mainly due to anthropogenic source, especially application of nitrogen fertilizers. Quantifying $N_2O$ emission from agricultural field is essential to develop national inventories of greenhouse gases (GHGs) emission. The objective of the study was to develop emission factor to estimate direct $N_2O$ emission from agricultural field in Gangwon-do, Korea by measuring $N_2O$ emissions from potato (Solanum tuberosum), red pepper (Capsicum annum L.), and Chinese cabbage (Brassica campestris L.) cultivation lands from 2009 to 2012. Accumulated $N_2O$ emission was $1.48{\pm}0.25kg$ $N_2O-N\;ha^{-1}$ for red pepper, $1.27{\pm}0.27kg$ $N_2O-N\;ha^{-1}$ for potato, $1.49{\pm}0.06kg$ $N_2O-N\;ha^{-1}$ for Chinese cabbage cultivated in spring, and $1.14{\pm}0.22kg$ $N_2O-N\;ha^{-1}$ for fall Chinese cabbage. Emission factor of $N_2O$ calculated from accumulated $N_2O$ emission, nitrogen fertilization rate, and background $N_2O$ emission was $0.0051{\pm}0.0016kg$ $N_2O-N\;ha^{-1}$ N for cropland in Gangwon province. More extensive study is deserved to be conducted to develop $N_2O$ emission factor for upland crops in Korea through examining the emission factors from various regions and crops because $N_2O$ emission is influenced by many factors including climate characteristics, soil properties, and agricultural practices.

• Journal of Plant Biotechnology
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• v.31 no.3
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• pp.191-195
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• 2004

Arabidopsis NDPK2 (AtNDPK2) is a key singaling component that regulate cellular redox state and known to enhance multiple stress tolerance when over-expressed in Arabidopsis plant (Moon et al. 2003). In order to develop transgenic potato plants with enhanced tolerance to multiple stresses, we placed an AtNDPK2 cDNA under the control of a stress-inducible SWPA2 promoter or enhanced CaMV 35S promoter. Transgenic potato plants (cv. Superior and Atlantic) were generated using an Agrobacterium-mediated transformation system and selected on MS medium containing 100 mg/L kanamycin. Genomic Southern blot analysis confirmed the incorporation of AtNDPK2 cDNA into the potato genome. When potato leaf discs were treated with methyl viologen (MV) at 10 $\mu$M, transgenic plants showed higher tolerance to MV than non-transgenic or vector-transformed plants. The NDPK2 transgenic potato plants will be further used for analysis of stress-tolerance to multiple environmental stresses.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.1
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• pp.68-73
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• 2002

High temperature during sprout emergence period of potato (Solanum tuberosum L.) is a major limitation to the yield of fall-grown potato in Korea. To explore the possibility of improving the yield of fall-grown potato through recycled paper mulching, the changes of soil environment and the growth and yield of potato cv. Daeji as affected by three mulching treatments of non-mulched control, recycled paper mulching (RPM), and black polyethylene film-mulching (BPFM) were examined over two fall seasons at Suwon, Korea. The mulching materials were a recycled mulch paper with 110 g/$m^2$ and a thickness of 0.1 mm, which was manufactured from old corrugated containers for this experiment and a commercial black polyethylene film with a thickness of 0.01mm. On an average throughout the growing period, the soil temperature with RPM at the 5-cm depth was lower by 0.4-1.1$^{\circ}C$ than that of the control. The maximum temperature during daytime in RPM was lowered by approximately 1 to 5$^{\circ}C$ according to the weather condition during emergence period, the difference being great on a fine day. The soil temperature with BPFM was much higher than the control. The effect of the mulching treatments diminished as the canopy became developed. The mulching treatments were more advantageous than the control in the conservation of soil water. Moreover, RPM and BPFM efficiently suppressed the occurrence of weeds until the potato harvest. RPM improved the emergence significantly due to lower soil temperature, whereas BPFM showed much poorer emergence than the control. Growth after emergence and yield were significantly higher in RPM than in the control and BPFM. It was concluded that the significantly improved yield in RPM compared to that in the control was mainly due to the improvement of the sprout emergence and tuber growth accompanied by lower soil temperature and better conservation of soil water.

• Journal of Plant Biotechnology
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• v.31 no.2
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• pp.109-113
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• 2004

In order to develop transgenic potato plants with enhanced tolerance to multiple stress, we constructed the transformation vector expressing both superoxide dismutase and ascorbate peroxidase genes in chloroplasts under the control of a stress-inducible SWPA2 promoter. Transgenic potato plants (cv. Superior and Atlantic) were generated using an Agrobacterium-mediated transformation system. Transgenic potato plants were regenerated on MS medium containing 100mg/L kanamycin. Genomic Southern blot analysis confirmed the incorporation of foreign genes into the potato genome. When potato leaf discs were subjected to methyl viologen (MV) at 10 $\mu$M, transgenic plants showed higher tolerance than non-transgenic or vector-transformed plants. To further study we selected the transgenic plant lines with enhanced tolerance against MV. These plants will be used for further analysis of stress-tolerance to multiple environmental stresses.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.103-109
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• 2013

Estimating the high tuber solids needs a simulation system on potato growth, and its development should be obtained by using agricultural elements which analyze the relationship between crop growth and agricultural factors. An accurate simulation to predict solids level against climatic change employs a calculation of in vivo energy consumption and bias for growth and induction shape in a slight environmental adaptation. So, to calculate in vivo energy consumption, this study took a concept of estimate of the amount of basal metabolism in each tuber. In the validation experiments, the results of measuring solid accumulation of potatoes harvested at dates suggested by simulation agreed with the actual measured values in each regional field during the growth period of years from 2006 till 2010. The mean values of tuber solids level and inter-annual level variation in validation experiments were predicted well by the simulation model. And also, the results of validation experiments represent that concentration of tuber solids were due mainly to the duration of sunshine, above 190 hours per a month, and the cumulative amount of radiation, above 2,200 $MJ{\cdot}m^{-2}$, of the effective growth period.

• Korean Journal of Plant Resources
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• v.30 no.5
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• pp.556-564
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• 2017

Appropriate soil temperature and early planting of potato is very important for the successful potato-soybean cropping system in central region of South Korea. This experiment was carried out to determine the effect of mulching materials on the growth and yield of potato (Solanum tuberosum L.). Five different mulch treatments were had been applied on an upland soil as follows ; no mulch (NM), transparent film (TF), transparent film + additional transparent film (TF + ATF), black film (BF), and black film + additional transparent film (ATF). In the period of sowing time to removing additional films, mean soil temperature of the treatments was in the order of TF+ATF > TF > BR+ATF > BF as $20.3^{\circ}C$ > $18.5^{\circ}C$ > $16.1^{\circ}C$ > $15.4^{\circ}C$, respectively and that of NM was $13.8^{\circ}C$. The accumulated soil temperature was TF > NM > BF during the removing additional films to earthing at inter-tillage. On the changes in the soil temperature during a whole day, the temperature in the BF was lower than NM during around 18:00 PM to 12:00 NM, while NM was higher than BF in the time period of 10:00AM to 21:00PM. The sequence of potato sprout emergence was 15 > 18 > 20 > 22 days of TF+ATF, TF, BF+ATF, and BF, respectively and that of NM was 24 days. Comparing to the NM, potato sprout emergence was observed on the TF+ATF treated plot as early as 9 days. At 10 days before harvest, the significant difference in the tuber dry weight had been observed and the sequence tuber weight was in the order of TF > TF+ATF > BF+ATF > BF > NM. The potato yields of TF, TF+ATF, and BF+ATF were increased of 40.7, 37.3, and 22% as compared to NM ($2,805kg\;10a^{-1}$), but almost same yield in the BF. The differences of tuber dry weight and potato yields was co-related with the temperature rise of soil by the application of mulching materials on soil. Based on these results, application of mulching film had been very effective to increase the tuber size and the yield of potato by the temperature rise during seedling stage of potato. Transparent mulching was better than black mulching especially for the emergence of sprout of potato in relation to minimizing cooling injury.