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http://dx.doi.org/10.5010/JPB.2004.31.3.191

Selection of Transgenic Potato Plants Expressing NDP Kinase 2 Gene with Enhanced Tolerance to Oxidative Stress  

Li, Tang (한국생명공학연구원 식물세포공학연구실)
Kwon, Suk-Yoon (한국생명공학연구원 환경생명공학연구실)
Yun, Dae-Jin (경상대학교 대학원 응용생명과학부)
Kwak, Sang-Soo (한국생명공학연구원 환경생명공학연구실)
Lee, Haeug-Soon (한국생명공학연구원 식물세포공학연구실)
Publication Information
Journal of Plant Biotechnology / v.31, no.3, 2004 , pp. 191-195 More about this Journal
Abstract
Arabidopsis NDPK2 (AtNDPK2) is a key singaling component that regulate cellular redox state and known to enhance multiple stress tolerance when over-expressed in Arabidopsis plant (Moon et al. 2003). In order to develop transgenic potato plants with enhanced tolerance to multiple stresses, we placed an AtNDPK2 cDNA under the control of a stress-inducible SWPA2 promoter or enhanced CaMV 35S promoter. Transgenic potato plants (cv. Superior and Atlantic) were generated using an Agrobacterium-mediated transformation system and selected on MS medium containing 100 mg/L kanamycin. Genomic Southern blot analysis confirmed the incorporation of AtNDPK2 cDNA into the potato genome. When potato leaf discs were treated with methyl viologen (MV) at 10 $\mu$M, transgenic plants showed higher tolerance to MV than non-transgenic or vector-transformed plants. The NDPK2 transgenic potato plants will be further used for analysis of stress-tolerance to multiple environmental stresses.
Keywords
Multiple stress; NDP kinase; oxidative stress; Solanum tuberosum; transformation;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Kwon SY, Choi SM, Ahn YO, Lee HS, Lee HB, Park YM, Kwak SS (2003) Enhanced stress-tolerance of transgenic tobacco plants expressing a human dehydroascorbate reductase gene. J Plant Physiol 160: 347-353   DOI   ScienceOn
2 Slooten L, Capiau K, Van Camp W, Van Montagu M, Sybesma C, Inze D (1995) Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overexpressing manganese superoxide dismutase in the chloroplasts. Plant Physiol 107: 737-750   DOI
3 Allen RD (1995) Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol 107: 1049-1054   DOI
4 Asemota HN (1995) A fast, simple, and efficient miniscale method ofr the preparation of DNA from tissues of yam (Dioscorea spp.). Plant Mol Biol Rep 1: 19-21   DOI
5 Choi G, Yi II, Lee J, Kwon YK, Soh MS, Shin B, Luka Z, Hahn TR, Song PS (1999) Phytochrome signalling is mediated through nucleoside diphosphate kinase 2. Nature 401: 610-613   DOI   ScienceOn
6 Inze D, Van Montagu M (1995) Oxidative stress in plants. Curr Opin Biotechnol 6: 166-172
7 Kim KY, Kwon SY, Lee HS, Hur YK, Bang JW, Kwak SS (2003) A novel oxidative stress-inducible peroxidase promoter from sweet potato: molecular cloning and characterization in transgenic tobacco plants and cultured cells. Plant Mol Biol 51: 831-838   DOI   ScienceOn
8 Kwon SY, Jeong YJ, Lee HS, Kim JS, Cho KY, Allen RD, Kwak SS (2002) Enhanced tolerances of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against methyl viologen-mediated oxidative stress. Plant Cell Environ 25: 873-882   DOI   ScienceOn
9 Martha L, Galvis E, Marttila S, Hakansson G, Forsberg J, Knorpp C (2001) Heat stress response in pea involves interaction of mitochondrial nucleoside diphosphate kinase with a novel 86-kilodalton protein. Plant Physiol 126: 69-77   DOI
10 McKersie BD, Bowlwy SR, Harjanto E, LePrince O (1996) Water-deficit tolerance and field performance of transgenic alfalfa overexpression superoxide dismutase. Plant Physiol 111: 1177-1181   DOI
11 Moon HJ, Lee BY, Choi G, Shin DJ, Prasad T, Lee OS, Kwak SS, Kim DH, Nam JS, Bahk JD, Hong JC, Lee SY, Cho MJ, Lim CO, Yun DJ (2003) NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. Proc Natl Acad Sci 100: 358-363   DOI   ScienceOn
12 Murashige T, Skoog F (1962) A rsvised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497   DOI
13 Pan L, Kawai M, Yano A, Uchimiya H (2000) Nucleoside diphosphate kinase required for coleoptile elongation in rice. Plant Physiol 122: 447-452   DOI   ScienceOn
14 Roxas VP, Smith RK, Jr Allen ER, Allen RD (1997) Overexpression of glutathione-S-transferase/glutathione peroxidase enhances the growth of transgenic tobacco seedlings during stress. Nat Biotechnol 15: 988-991   DOI   ScienceOn
15 Yun BW, Huh GH, Lee HS, Kwon SY, Jo JK, Kim JS, Cho KY, Kwak SS (2000) Differential resistance to methyl viologen in transgenic tobacco plants that express sweetpotato peroxidases. J Plant Physiol 156: 504-509   DOI   ScienceOn
16 Tang Li, Kwon SY, Sung CK, Kwak SS, Lee HS (2004) Selection of transgenic potato plants expressing both CuZnSOD and APX in chloroplasts with enhanced tolerance to oxidative stress. Korean J Plant Biotechnol 31: 109-113   DOI   ScienceOn
17 Yang KA, Moon HJ, Kim GT, Lim CJ, Hong JC, Lim CO, Yun DJ (2003) NDPK kinase 2 regulates expression of antioxidant genes in Arabidopsis. Proc Japan Acad 79B: 86-91   DOI   ScienceOn
18 Youm JW, Jeon JH, Jung JY, Lee BC, Kang WJ, Kim MS, Kim CJ, Joung H, Kim HS (2002) Production of VP6 gene into potato plants by Agrobacterium-mediated transformation and analysis of VP6 expression in transgenic potatoes. Korean J Plant Biotechnol 29: 93-98   DOI   ScienceOn
19 Tang Li, Kwon SY, Kwak SS, Sung CK, Lee HS (2003) Susceptibility of two cultivars to various environmental stresses. Korean J Plant Biotechnol 30: 405-410   DOI   ScienceOn