• 한국수정란이식학회지
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• 제24권4호
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• pp.293-295
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• 2009

Two postmortem male and female reticulated giraffes were examined. The adult male giraffe showed sigmoid flexure of penis similar to most ungulates. Epididymis was well-developed and divided with head, body and tail parts. On the tip of penis, there was a urethral process. At the necropsy of a 20-month-old and nulliparous giraffe, ovaries, oviducts, two uterine horns with a septum and a cervix were distinctively shown. Understanding reproductive organs of giraffes would be beneficial to succeed in artificial breeding on this species especially in the difficult situation of importing hoofed animals.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.170.1-170.1
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• 2003

Carisoprodol(CSP), commonly prescribed as a skeletal muscle relaxant, is increasingly abused among young people for a recreational purpose in Korea. CSP may induce hallucination if it were ingested with large amounts, futhermore a carisoprodol overdose is considered fatal. Recently, we encountered overdoses of carisoprodol in 6 suicide cases. (omitted)

• 농업과학연구
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• 제43권1호
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• pp.109-115
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• 2016

Silk has had a reputation as a luxurious and sensuous fabric but it is not popular due to the expensive price and poor durability. To develop the silk materials that apply the various industries, the artificially synthesized gene can be introduced into the silkworm and expressed in the silk gland. Transgenic silkworms for the mass production of green fluorescent silks are generated using a fibroin H-chain expression system. For commercial use, safety assessment of the transgenic silkworms is essential. The purpose of this study was to examine the potential acute oral toxicity of EGFP protein expressed in genetically modified (GM) fluorescence silkworm and to obtain the approximative lethal dose in the male and female at 6-weeks ICR mice. EGFP protein was fed at a dose of 2,000 mg/kg body weight in five male or five female mice. Mortalities, clinical findings and body weight changes were monitored for 1, 3, 7, 14 days after dosing. At the end of 14 day observation period, all mice were sacrificed, and the postmortem necropsy were performed. The test group was not observed death case. Also the effect was not admitted by test substance administration in common symptoms, the body weight and postmortem. The results of single-dose oral toxicity test showed that approximative lethal dose of EGFP protein expressed in fluorescence silkworm was considered to exceed the 2,000 mg/kg body weight in both sexes.

• 한국축산식품학회지
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• 제36권3호
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• pp.343-351
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• 2016

The objective of this study was to evaluate the effects of a short-term/extreme temperature abuse (STA) on color characteristics and oxidative stability of aged beef muscles during simulated retail display. Two beef muscles (longissimus lumborum, LL and semitendinosus, ST) were aged for 7 (A7), 14 (A14), 21 (A21), and 28 d (A28), and further displayed at 2℃ for 7 d. The STA was induced by placing steak samples at 20℃ for 1 h on the 4th d of display. Instrumental and visual color evaluations, ferric ion reducing capacity (FRC) and 2-thiobarbituric acid reactive substances (TBARS) assay were performed. Initially, redness, yellowness and hue angle of all beef muscles were similar, regardless of aging time before display (p>0.05). An increase in postmortem aging time increased lipid oxidation and caused a rapid discoloration after STA during display (p<0.05). ST muscle was more sharply discolored and oxidized after STA, when compared to LL muscle (p<0.05). The FRC value of beef muscles was decreased after 7 d of display (p<0.05). The results from the current study indicate adverse impacts of postmortem aging on color and oxidative stabilities of beef muscles, particularly under temperature abusing conditions during retail display. Thus, developing a specific post-harvest strategy to control quality attributes in retail levels for different muscle types and aging conditions would be required.

• 한국축산식품학회지
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• 제38권4호
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• pp.703-710
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• 2018

Bromodomain-containing protein 2 (BRD2) is a nuclear serine/threonine kinase involved in transcriptional regulation. We investigated the expression and association of the BRD2 gene as a candidate gene for meat quality traits in Berkshire pigs. BRD2 mRNA was expressed at relatively high levels in muscle tissue. Statistical analysis revealed that the c.1709G>C polymorphism of the BRD2 gene was significantly associated with carcass weight, meat color ($a^*$, redness), protein content, cooking loss, water-holding capacity, carcass temperatures 4, 12 and 24 h postmortem, and the 24 h postmortem pH in 384 Berkshire pigs. Therefore, this polymorphism in the porcine BRD2 gene may be used as a candidate genetic marker to improve meat quality traits in pigs.

• 대한임상독성학회지
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• 제1권1호
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• pp.34-39
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• 2003

Carisoprodol (CSP) is commonly prescribed as a skeletal muscle relaxant. Recently, we encountered 7 suicidal cases in which carisoprodol was detected. We developed a rugged, sensitive, and specific method for the determination of CSP and meprobamate (MPB) by GC and GC/MS. Postmortem blood concentrations of CSP and MPB ranged 22.9-124.4 ,$\mu$g/ml and its metabolite, 26.8-144.5 ,$\mu$g/ml respectively. Among 7 cases studied, Only CSP was ingested in 4 cases and combination of CSP and dextromethorphan was ingested in 2 cases according to the case history and one case was with ethanol. The order of the tissue concentration of CSP and MPB was liver> kidney > brain, and the concentration of MPB was higher than that of CSP in all tissues. The MPB /CSP concentration ratios of urine, bile juice, liver, kidney, brain and blood were 15.7, 4.0, 1.2, 1.4, 1.4 and 1.0 respectively. There was a big difference in concentration of CSP and MPB in 7 cases due to differences in the amount of dose administered and time to death after dosing.

• 분석과학
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• 제12권6호
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• pp.577-582
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• 1999

혈액 중에서 톨루엔을 HS-SPME 법을 이용하여 GC/MSD로 분석하였으며, 부탄가스류의 분석은 HS 법을 이용하여 CC/FID로 분석하였다. 우리 나라의 경우 지난 3년 동안 청소년들이 본드류나 부탄가스류 등을 흡입한 상태에서 사망한 경우가 총 75건이 발생하였다. 본드류를 흡입하다가 사망한 사람은 27명이었으며, 그 중에서 9명은 톨루엔 중독상태에서 자제력의 상실 등으로 인하여 고층에서 떨어지는 추락사를 하였으며, 이때의 혈중 농도 분포는 $1.3{\sim}21.6{\mu}g/mL$으로 상당히 높은 농도였다. 또한 부탄가스를 흡입하다가 질식 사망 또는 흡입 사망한 사람은 54명이었으며, 이 중에는 본드류와 부탄가스를 동시에 흡입하다가 사망한 6명도 포함되었다.

• 한국식품영양학회지
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• 제10권3호
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• pp.401-406
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• 1997

소의 도체로부터 사태, 갈비 및 등심을 분리하고 8$^{\circ}C$에 저장하면서 actomyosin을 추출하여 부위별 저장기간에 따라 추출성 및 ATPase활성을 비교하였다. Actin과 myosin이 유리되어 형성된 actomyosin의 추출성은 저장초기 사태, 갈비 및 등심이 각각 36.74, 72.55 및 56.77mg/g이었으며, 저장기간에 의한 추출양상은 갈비와 등심이 비슷하였고, 사태는 이들과 다르게 진행되었다. 사태의 Mg- 및 Ca-ATPase활성은 저장 3일까지 상승하다가 6일째 감소하였고, 갈비는 저장기간 동안 비슷하였으며, 등심은 저장기간에 따라 조금씩 낮아지는 경향이었다. 그리고 Mg- 및 Ca-ATPase활성은 사태, 등심 및 갈비의 순으로 크게 나타났다. 사태와 갈비의 EDTA-ATPase활성은 저장기간과 이온강도에 따라 차이를 보였지만 등심은 이온강도가 커짐에 따라 계속 상승하였다.

• 한국축산식품학회지
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• 제25권4호
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• pp.423-429
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• 2005

돈육을 수세하여 돈육 수리미를 제조할 때, 사후 해당 속도가 빠른 돈육을 원료육으로 이용하면 낮은 pH에 기인하여 보수성이 낮은 결과 적은 수분 함량을 보유하는 돈육 수세물을 획득하게 되어 수율이 낮아졌다. 사후 해당 속도가 빠른 돈육은 정상 돈육에 비해 육단백질의 변성이 유발되어 수분 함량이 낮고 치밀한 젤 매트릭스를 형성하여 경도가 높지만 탄력성이 낮은 돈육 수리미를 생산하였다. 뿐만 아니라 사후 해당 속도가 빠른 돈육은 변성된 근장 단백질이 근원섬유 단백질과 결합하여 수세되지 않고 돈육 수세물 내에 잔존하게 되어 돈육 수리미의 색깔을 어둡게 만드는 원인으로 작용한 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제26권6호
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• pp.879-885
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• 2013

Fifty-four, mixed-sex, halothane-carrier crossbred (Yorkshire${\times}$Landrace) pigs with an average initial BW of $108.2{\pm}0.8$ kg were randomly allotted to one of three dietary treatments for 5 d before slaughter: i) a control corn-soybean meal finisher diet devoid of supplemental magnesium; ii) a diet supplemented with 1.5 g/kg of elemental Mg from magnesium acetate; and iii) a diet supplemented with 1.5 g/kg of elemental Mg from magnesium sulfate heptahydrate. Serum creatine kinase (CK), lactate and glucose were analyzed at slaughter. Muscles from longissimus (LM) were packaged and stored to simulate display storage for muscle lactate and glycogen determinations at 0, 1, 2, 3, and 4 d. Mg supplementation reduced (p<0.05) serum CK and lactate concentration, but had no effect (p>0.05) on serum glucose. Daily change of muscle lactate concentration linearly increased (p<0.01), while glucose concentration linearly decreased (p<0.05) as storage time increased in all treatments. However, dietary Mg acetate and Mg sulfate supplementation in pigs elevated (p<0.05) muscle glycogen and reduced (p<0.05) muscle lactate concentrations, especially during the first 2 d of display, compared with pigs fed the control diet. This study suggests that short-term feeding of magnesium acetate and magnesium sulfate to heterozygous carriers of the halothane gene has beneficial effects on stress response and pork quality by improving blood and muscle biochemical indexes.