• 대한의생명과학회지
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• 제16권3호
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• pp.193-196
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• 2010

Multiplex PCR-based short tandem repeat (STR) analysis is considered as a good tool for monitoring bone marrow engraftment after sex-mismatched allogeneic transplantation and provides a sensitive and accurate assessment of the contribution of both donor and/or recipient cells in post-transplantation specimens. Forensic STR analysis and quantitative real time PCR are used to determine the proportion of donor versus recipient each contained within the total DNA. The STR markers were co-amplified in a single reaction by using commercial $PowerPlex^{(R)}$ 16 system and $AmpFISTR^{(R)}$ $Identifiler^{(R)}$ / $Yfiler^{(R)}$ PCR amplification kits. Separation of the PCR products and fluorescence detection were performed by ABI $PRIS^{(R)}$ 3100 Genetic Analyzer with capillary electrophoresis. The $GeneMapper^{TM}$ ID software were used for size calling and analysis of STR profiles. Extracted DNA was quantified by the $Quantifiler^{TM}$ Human DNA / Y Human Male DNA Quantification Kit The intent of this study was to analyze the ratio of donor versus recipient cells in the post-transplant peripheral blood, spleen, lung and kidney specimens. Specimens were taken from the traffic accident male victim who had been engrafted from bone marrow female donor. Blood and spleen specimens displayed female donor DNA profile. Kidney specimen showed male recipient DNA profile. Interestingly, lung tissue showed mixed profiles. The findings of this study indicate that the forensic STR analysis using fluorescence labeling PCR combined with capillary electrophoresis is quick and reliable enough to assess the ratio of donor versus recipient cells and to monitor the mixed chimeric patterns.

• 감성과학
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• 제22권3호
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• pp.55-64
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• 2019

Stroke is a health problem experienced by many elderly people around the world. Stroke has a devastating effect on quality of life, causing death or disability. Hemiplegia is clearly an early sign of a stroke and can be detected through patterns of body balance and gait. The goal of this study was to determine various feature vectors of foot pressure and gait parameters of patients with stroke through the use of a wearable sensor and to compare the gait parameters with those of healthy elderly people. To monitor the participants at all times, we used a simple measuring device rather than a medical device. We measured gait data of 220 healthy people older than 65 years of age and of 63 elderly patients who had experienced stroke less than 6 months earlier. The center of pressure and the acceleration during standing and gait-related tasks were recorded by a wearable insole sensor worn by the participants. Both the average acceleration and the maximum acceleration were significantly higher in the healthy participants (p < .01) than in the patients with stroke. Thus gait parameters are helpful for determining whether they are patients with stroke or normal elderly people.

• 천문학회보
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• 제38권1호
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• pp.59.1-59.1
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• 2013

HBC722 (also known as LkHa 188-G4 and PTF 10qpf; A. Miller et al., 2011) is one of the FU Orionis-like young stellar objects which outbursted in August 2010 (Semkov et al., 2010). We have been monitoring the post-outburst phase of this object since November 2010 with Korean Astronomy and Space Science Institute Near Infrared Camera System (KASINICS), at Bohyunsan Optical Astronomy Observatory (BOAO). Four filters, J, H, Ks, and H2 band, were used for this observation. We did aperture photometry to find photometric variation. The light curve shows a long period brightness change. After decrease of the brightness, which was reported at the KAS 2011 fall meeting, HBC722 brightens up slowly now. However we cannot confirm any short period variations, previously reported by Green et al (2013), due to large scatters in the obtained light curve.

• 산업경영시스템학회지
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• 제34권3호
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• pp.24-34
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• 2011

An Unmanned Aerial Vehicle (UAV) is a powered pilotless aircraft, which is controlled remotely or autonomously. UAVs are an attractive alternative for many scientific and military organizations. UAVs can perform operations that are considered to be risky or uninhabitable for human. UA V s are currently employed in many military missions such as reconnaissance, surveillance, enemy radar jamming, decoying, suppression of enemy air defense (SEAD), fixed and moving target attack, and air-to-air combat. UAVs also are employed in a number of civilian applications such as monitoring ozone depletion, inclement weather, traffic congestion, and taking images of dangerous territory. For accomplishing the UAV's missions, guarantee of survivability should be preceded. The main objective of this study is to suggest a mathematical programming model and a $A^*PS$-PGA (A-star with Post Smoothing-Parallel Genetic Algorithm) for an UAV's path planning to maximize survivability. A mathematical programming model is composed by using MRPP (Most Reliable Path Problem) and TSP (Traveling Salesman Problem). A path planning algorithm for UAV is applied by transforming MRPP into SPP (Shortest Path Problem).

• 농촌계획
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• 제25권2호
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• pp.99-117
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• 2019

Environmental impact assessment (EIA), which predicts, evaluates, and manages the influences on natural landscape, plays a role of monitoring natural resources for systematic management of natural landscape. However, the function of verification and correction of the system is still insufficient and feed-back, one of the most important features of EIA follow-up, has not been introduced in Korea's EIA system yet. As a procedure, it is required to check if the opinions of the evaluators are properly reflected to the outcomes of the project through a reviewing process after assessing environmental impacts of a development project. In reality, despite the awareness about the importance of follow-up inspection of the conformity with, the system mainly focuses on the agreement during the planning stage of the development project and fails to continuously manage after its completion. There have been various preceding studies related to prediction, evaluation, and management of environmental impacts on natural landscape for better management. They primarily dealt with the problems in the EIA process and suggested improvement measures, including directions for institutional development, step-by-step goals, and operation methods, to address the problems which arise in the EIA follow-up process. However, suggested measures are not actively applied with the focus only put on institutional operation, there are virtually no standardized methods to predict and assess landscape changes due to the development project and to manage landscape after the project. Against this backdrop, this study aims to explore the existing methods to analyze the impacts natural landscape and to establish a system where landscape management is continued after the development project. To this end, we will suggest reducing methods according to the predicted changes in landscape for post-project management of natural landscape. Characteristics of reduction methods by project type were examined through reviewing the guide to natural landscape rating and the importance of development project impacts on natural landscape by type of reduction was evaluated through questionnaire for experts. Evaluated types of reduction are classified and presented by characteristics of each development project and content of reduction type.

• 대한의용생체공학회:의공학회지
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• 제37권5호
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• pp.178-185
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• 2016

Tear is a promising biological fluid for non-invasive health monitoring. It has been studied in the past to be a possible candidate for the diagnosis of certain systemic diseases, such as breast cancer, multiple sclerosis, prostate cancer, and diabetes. However, currently existing methods for collecting and extracting tear from the human eye causes inconsistencies in the biomolecule concentrations of the tear sample due to the irritating nature of the process. In response, we designed and fabricated a microfluidic system embedded soft contact-lens for the purpose of tear sampling. The lens was then tested with artificial tear for its tear sampling capability, and found to be able to find concentration equilibrium within 50 minutes. Additionally, simulation was carried out to further optimize the design so that tear sampling rate matched the natural tear turn-over rate of 1 microliter per minute.

• 한국컴퓨터정보학회논문지
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• 제24권6호
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• pp.109-115
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• 2019

Smart Factory is a part of and a key point of the 4th industrial revolution. It performs optimization from the whole viewpoint, using comprehensive data of the post-process data by utilizing various sensors, controllers, and mobile devices beyond the existing factory automation level. In this paper, we design and implement an IoT platform that can detect the safety factors of the workers, the environmental factors of the factory, and real time monitoring at the control center, among the fields to implement smart factory. To accomplish this, we construct a monitoring device that provides sensor information control, server transmission of sensor information, and visualization of collected information. By using this system, it is possible to maintain the temperature and humidity for the optimum working environment in the factory. and also, By using the beacon, it is possible to measure the working time of the worker and trace the position.

• Mass Spectrometry Letters
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• 제12권3호
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• pp.85-92
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• 2021

The therapeutic antibody drug market has experienced explosive growth as mAbs become the main therapeutic modality for a variety of diseases. Characterization of glycosylation that directly affects the efficacy and safety of therapeutic monoclonal antibodies (mAbs) is critical for therapeutics development, bioprocess system optimization, lot release, and comparability evaluation. The LC/MS approach has been widely used to structurally characterize mAbs, and recently attempts have been made to obtain comprehensive information on the primary structure and post-translational modifications (PTMs) of mAbs through intact protein analysis. In this study, we performed state-of-the-art LC/MS based intact protein analysis to readily identify and characterize glycoforms of various mAbs. Different glycoforms of mAbs produced in different expression cell lines including CHO, SP2/0 and HEK cells were monitored and compared. In addition, the comparability of protein molecular weight, glycoform pattern, and relative abundances of glycoforms between the commercialized trastuzumab biosimilar and the original product was determined in detail using the given platform. Intact mAb analysis allowed us to gain insight into the overall mAb structure, including the complexity and diversity of glycosylation. Furthermore, our analytical platform with high reproducibility is expected to be widely used for biopharmaceutical characterization required at all stages of drug development and manufacturing.

• 대한원격탐사학회지
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• 제36권6_1호
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• pp.1303-1322
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• 2020

Indonesia is more prone to natural disasters due to its geological condition under the three main plates, making Indonesia experience frequent seismic activity, causing earthquakes, volcanic eruption, and tsunami. Those disasters could lead to other disasters such as landslides, floods, land subsidence, and coastal inundation. Monitoring those disasters could be essential to predict and prevent damage to the environment. We reviewed the application of remote sensing and Geographic Information System (GIS) for detecting natural disasters in the case of Indonesia, based on 43 articles. The remote sensing and GIS method will be focused on InSAR techniques, image classification, and susceptibility mapping. InSAR method has been used to monitor natural disasters affecting the deformation of the earth's surface in Indonesia, such as earthquakes, volcanic activity, and land subsidence. Monitoring landslides in Indonesia using InSAR techniques has not been found in many studies; hence it is crucial to monitor the unstable slope that leads to a landslide. Image classification techniques have been used to monitor pre-and post-natural disasters in Indonesia, such as earthquakes, tsunami, forest fires, and volcano eruptions. It has a lack of studies about the classification of flood damage in Indonesia. However, flood mapping was found in susceptibility maps, as many studies about the landslide susceptibility map in Indonesia have been conducted. However, a land subsidence susceptibility map was the one subject to be studied more to decrease land subsidence damage, considering many reported cases found about land subsidence frequently occur in several cities in Indonesia.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
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• pp.3226-3236
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• 2011

In this paper, with the background features on which measuring the transverse left-right and up -down vibration of wheel, bogie and body by wireless measurement system, performance evaluation systems which can assess the running behavior of high speed trains based on UIC code 518-OR and evaluate the ride comfort of them based on ISO code 2631 and UIC code 513 were developed. The characteristics of dynamic vibration are generally analyzed by an acceleration of a car body of high speed train and the acceleration can be applied to evaluation of running safety. In this paper, also matching system of distance from tachometer and vibration from accelerometer was programmed in development software, and the SD card embedded system which prevent to loss of data in wireless measurement was mounted on this system. Finally the software perform to analysis with filtering and statistical post-process in the unit sections and zones and focus on developing the capability monitoring in the main control center. For the verification of this system, the running behavior and safety factor were analyzed based on field measured data of the Cheonjun-gunnum-sun turn out point in the new KTX railway.